Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers.

Genetic diversity and patterns of population structure of the 94 oil palm lines were investigated using species-specific simple sequence repeat (SSR) markers. We designed primers for 63 SSR loci based on their flanking sequences and conducted amplification in 94 oil palm DNA samples. The amplification result showed that a relatively high level of genetic diversity was observed between oil palm individuals according a set of 21 polymorphic microsatellite loci. The observed heterozygosity (Ho) was 0.3683 and 0.4035, with an average of 0.3859. The Ho value was a reliable determinant of the discriminatory power of the SSR primer combinations. The principal component analysis and unweighted pair-group method with arithmetic averaging cluster analysis showed the 94 oil palm lines were grouped into one cluster. These results demonstrated that the oil palm in Hainan Province of China and the germplasm introduced from Malaysia may be from the same source. The SSR protocol was effective and reliable for assessing the genetic diversity of oil palm. Knowledge of the genetic diversity and population structure will be crucial for establishing appropriate management stocks for this species.

Removal of Cr from tannery sludge by indigenous sulfur-oxidizing bacteria.

The aim of the present study was to examine Cr removal from tannery sludge by bioleaching method using indigenous sulfur-oxidizing bacteria with special emphasis on the influence of bioleaching process on sludge settleability. Chemical leaching with sulfuric acid was designed as the control. The results showed that the inoculation of sulfur-oxidizing bacteria and the addition of elemental sulfur were effective in removing Cr from tannery sludge. After 144 hours of bioleaching, 98% of Cr could be leached. Although it took only 8 hours to reduce the sludge pH from 7.8 to about 2.0 by chemical leaching as compared to 144 hours for bioleaching treatment, chemical leaching removed only 91% of the total Cr. Regardless of bioleaching and chemical leaching treatments, sludge settleability improved considerably with a decrease in sludge pH. Bioleaching treatment performed better than chemical leaching in terms of the percentage of settled sludge and the effluent suspended solids (ESS) content in sludge supernatant. Nevertheless, further work should be carried out to investigate the precise mechanisms leading to such enhanced sludge settleability during sludge bioleaching process.