ABSTRACT
Role of circ-FNTA in the progression of bladder cancer (BCa) and its underlying mechanism were investigated. circ-FNTA level in BCa tissues and cell lines was detected. The prognostic potential of circ-FNTA was assessed by Kaplan-Meier methods and the proliferative and invasive abilities of BCa influenced by circ-FNTA were explored. Through dual-luciferase reporter gene assay, miRNA-451a, the target of circ-FNTA and the target gene of miRNA-451a, S1PR3 were determined. circ-FNTA was upregulated in BCa, especially in invasive BCa. High level of circ-FNTA indicated worse prognosis in BCa patients. Silence of circ-FNTA attenuated the proliferative and invasive abilities of T24 and UM-UC-3 cells. miRNA-451a was verified to be the target of circ-FNTA, which was downregulated in BCa cells. circ-FNTA negatively regulated the expression level of miRNA-451a. Moreover, S1PR3 was the downstream gene of miRNA-451a. Overexpression of miRNA-451a downregulated S1PR3 level in BCa cells. circ-FNTA accelerates the proliferative and invasive abilities of BCa through targeting miRNA-451a/S1PR3 axis, and indicates a poor prognosis of BCa patients.
ABSTRACT
PURPOSE: To compare and analyze the efficacy and safety of transurethral partial cystectomy with 2.0 µm laser and transurethral resection of bladder tumor (TURBT) in treating patients with superficial bladder cancer. METHODS: The clinical data of 130 patients with superficial bladder cancer were divided into two groups based on different treatments, with 65 patients in each group, and treated with transurethral partial cystectomy with 2.0 µm laser and TURBT separately. Then, operation conditions such as intraoperative blood loss, operation time, in-dwelling time of urinary catheter and length of hospital stay were recorded and compared between the two groups. Finally, the tumor recurrence in the patients was followed up and recorded. RESULTS: The operation time (p<0.001) and length of hospital stay (p=0.013) were remarkably shorter, and the intraoperative blood loss (p<0.001) was notably smaller in laser group than those in TURBT group. Laser group had an evidently lower total incidence rate of complications than TURBT group (p=0.005). The patients were reexamined by cystoscopy at 4 weeks after operation, and the biopsy results indicated that there were markedly more cases of positive findings in TURBT groupthan those in laser group (no positive findings) (p=0.033). However, laser group exhibited distinctly decreased postoperative levels of IL-6 and TNF-α but an obviously increased IL-10 level compared with TURBT group (p<0.001). Besides, after 6-40 months of follow-up for all the patients, the total recurrence rate was prominently lower in laser group than that in TURBT group (p=0.006). CONCLUSIONS: In contrast with TURBT, transurethral partial cystectomy with 2.0 µm laser for superficial bladder cancer can significantly reduce the operation time and intraoperative blood loss, improve the operative effect, induce fewer postoperative complications and cause milder body injury and inflammatory response at the same time, which is worthy of clinical promotion.
