Senescence-related genes analysis in breast cancer reveals the immune microenvironment and implications for immunotherapy.

Despite the advent of precision therapy for breast cancer (BRCA) treatment, some individuals are still unable to benefit from it and have poor survival prospects as a result of the disease's high heterogeneity. Cell senescence plays a crucial role in the tumorigenesis, progression, and immune regulation of cancer and has a major impact on the tumor microenvironment. To find new treatment strategies, we aimed to investigate the potential significance of cell senescence in BRCA prognosis and immunotherapy. We created a 9-gene senescence-related signature. We evaluated the predictive power and the role of signatures in the immune microenvironment and infiltration. In vitro tests were used to validate the expression and function of the distinctive critical gene ACTC1. Our risk signature allows BRCA patients to receive a Predictive Risk Signature (PRS), which may be used to further categorize a patient's response to immunotherapy. Compared to conventional clinicopathological characteristics, PRS showed strong predictive efficacy and precise survival prediction. Moreover, PRS subgroups were examined for altered pathways, mutational patterns, and possibly useful medicines. Our research offers suggestions for incorporating senescence-based molecular classification into risk assessment and ICI therapy decision-making.

Development and validation of prediction models for the prognosis of colon cancer with lung metastases: a population-based cohort study.

Background: Current studies on the establishment of prognostic models for colon cancer with lung metastasis (CCLM) were lacking. This study aimed to construct and validate prediction models of overall survival (OS) and cancer-specific survival (CSS) probability in CCLM patients. Method: Data on 1,284 patients with CCLM were collected from the Surveillance, Epidemiology, and End Results (SEER) database. Patients were randomly assigned with 7:3 (stratified by survival time) to a development set and a validation set on the basis of computer-calculated random numbers. After screening the predictors by the least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression, the suitable predictors were entered into Cox proportional hazard models to build prediction models. Calibration curves, concordance index (C-index), time-dependent receiver operating characteristic (ROC) curves, and decision curve analysis (DCA) were used to perform the validation of models. Based on model-predicted risk scores, patients were divided into low-risk and high-risk groups. The Kaplan-Meier (K-M) plots and log-rank test were applied to perform survival analysis between the two groups. Results: Building upon the LASSO and multivariate Cox regression, six variables were significantly associated with OS and CSS (i.e., tumor grade, AJCC T stage, AJCC N stage, chemotherapy, CEA, liver metastasis). In development, validation, and expanded testing sets, AUCs and C-indexes of the OS and CSS prediction models were all greater than or near 0.7, which indicated excellent predictability of models. On the whole, the calibration curves coincided with the diagonal in two models. DCA indicated that the models had higher clinical benefit than any single risk factor. Survival analysis results showed that the prognosis was worse in the high-risk group than in the low-risk group, which suggested that the models had significant discrimination for patients with different prognoses. Conclusion: After verification, our prediction models of CCLM are reliable and can predict the OS and CSS of CCLM patients in the next 1, 3, and 5 years, providing valuable guidance for clinical prognosis estimation and individualized administration of patients with CCLM.

Combination of Chinese herbal medicine and conventional western medicine for coronavirus disease 2019: a systematic review and meta-analysis.

Objective: This study aimed to assess the efficacy and safety of Chinese herbal medicine (CHM) plus conventional western medicine (CWM) in comparison with CWM against COVID-19. Methods: We searched eight electronic databases and three trial registers spanning from January 1, 2020 to May 18, 2023. We included randomized controlled trials (RCTs) comparing the effectiveness and safety of CHM plus CWM and CWM against COVID-19 in our study. The Cochrane Risk of Bias tool 2.0 (RoB2) was applied to evaluate the methodological quality of the included RCTs. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) system was employed to assess the certainty of evidence. Statistical analysis was implemented in R version 4.1.2. Results: Our study included 50 RCTs involving 11,624 patients. In comparison with sole CWM, CHM plus CWM against COVID-19 significantly enhanced clinical effective rate (RR = 1.18, 95% CI [1.13, 1.22]), improved chest image (RR = 1.19, 95% CI [1.11, 1.28]), inhibited clinical deterioration (RR = 0.45, 95% CI [0.33, 0.60]), lowered mortality (RR = 0.53, 95% CI [0.40, 0.70]), and reduced the total score of TCM syndrome (SMD = -1.24, 95% CI [-1.82, -0.66]). SARS-CoV-2 nucleic acid conversion time (MD = -2.66, 95% CI [-3.88, -1.44]), duration of hospitalization (MD = -2.36, 95% CI [-3.89, -0.82]), and clinical symptom (fever, cough, fatigue, and shortness of breath) recovery times were shorter in CHM plus CWM groups than in CWM groups. Further, CHM plus CWM treatment was more conducive for some laboratory indicators returning to normal levels. No statistical difference was found in the incidence of total adverse reactions between the two groups (RR = 0.97, 95% CI [0.88, 1.07]). We assessed the risk of bias for 246 outcomes, and categorized 55 into "low risk", 151 into "some concerns", and 40 into "high risk". Overall, the certainty of the evidence ranged from moderate to very low. Conclusions: Potentially, CHM listed in this study, as an adjunctive therapy, combining with CWM is an effective and safe therapy mode for COVID-19. However, more high-quality RCTs are needed to draw more accurate conclusions. Clinical trial registration: https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=293963.

Photonic-assisted multiple microwave frequency measurement with improved robustness.

A multiple microwave frequency measurement approach based on frequency-to-time mapping (FTTM) is reported. The FTTM is constructed by optical sideband sweeping and electric-domain intermediate frequency envelope monitoring. Two optimized operations are implemented. First, the use of balanced photodetection cancels out the beat components generated by the signals under test (SUT) themselves, so as to exclude frequency misjudgment. Second, a reference signal is introduced to map the SUT frequency to a relative time difference instead of an absolute time value, avoiding the measuring bias caused by time synchronization. As a result, the proposed scheme with improved robustness could be attractive for future practical applications. An experiment is performed. Microwave frequency measurement from 16 to 26 GHz is demonstrated, with an average error of 7.53 MHz.

Photonic-Assisted Scheme for Simultaneous Self-Interference Cancellation, Fiber Dispersion Immunity, and High-Efficiency Harmonic Down-Conversion.

A photonic approach to the cancellation of self-interference in the optical domain with fiber dispersion immunity and harmonic frequency down-conversion function is proposed based on an integrated, dual-parallel, dual-drive Mach-Zehnder modulator (DP-DMZM). A dual-drive Mach-Zehnder modulator (DMZM) is used as an optical interference canceller, which cancels the self-interference from the impaired signal before fiber transmission to avoid the effect of fiber transmission on the cancellation performance. Another DMZM is used to provide carrier-suppressed, local-oscillation (LO)-modulated, high-order double optical sidebands for harmonic frequency down-conversion to release the strict demand for high-frequency LO sources. By regulating the DC bias of the main modulator, the signal of interest (SOI) can be down-converted to the intermediated frequency (IF) band after photoelectric conversion with improved frequency-conversion efficiency, immunity to the fiber-dispersion-induced power-fading (DIPF) effect, and effective signal recovery. Theoretical analyses and simulation results show that the desired SOI in the X and K bands with a bandwidth of 500 MHz and different modulation formats can be down-converted to the IF frequency. The self-interference noise with the 2 GHz bandwidth is canceled, and successful signal recovery is achieved after a 10 km fiber transmission. The recovery performance of down-converted signals and the self-interference cancellation depth under different interference-to-signal ratios (ISRs) is also investigated. In addition, the compensation performance of DIPF is verified, and a 6 dB improvement in frequency conversion gain is obtained compared with previous work. The proposed scheme is compact, cost-effective, and thus superior in wideband self-interference cancellation, long-range signal transmission, and effective recovery of weak desired signals.

A highly sensitive ratiometric fluorescent probe based on fluorescein coumarin for detecting hydrazine in actual water and biological samples.

Hydrazine (N2 H4 ) is a highly toxic and harmful chemical reagent. Fluorescent probes are simple and efficient tools for sensitive monitoring of N2 H4 enrichment in the environment, humans, animals, and plants. In this work, a ratiometric fluorescent probe (FP-1) containing coumarin was used for hydrazine detection. The proposed FP-1 probe had a linear detection range of 0-250 µM and a limit of detection (LOD) of 0.059 µM (1.89 ppb). A large red Stokes shift was observed in fluorescence and UV-vis absorption spectra due to the hydrolysis of ester bonds between FP-1 and hydrazine. The hydrazine detection mechanism of FP-1 was also investigated using density functional theory (DFT) calculations. Finally, FP-1 could sensitively and selectively monitor hydrazine in actual water samples and BEAS-2B cells. Therefore, it has great application potential in environmental monitoring and disease diagnosis.

Estimation of positioning sparsity for Sagnac correction in fiber-optic time transfer.

The Sagnac effect is an important factor that leads to nonreciprocity in long-haul fiber-optic time and a frequency transfer system. For high-precision time transfer, correction must be performed to eliminate the time difference based on the trajectory of the path. However, the routing information may be not detailed enough to guarantee sufficient precision for Sagnac correction. Thus, nodes along the path must be surveyed with a certain sparsity. We provide a practical method for estimating the average distance of these nodes. Six simulated paths are generated to validate the method for different uncertainties.

The relationship between photometric and spectroscopic oscillation amplitudes from 3D stellar atmosphere simulations.

We establish a quantitative relationship between photometric and spectroscopic detections of solar-like oscillations using ab initio, 3D, hydrodynamical numerical simulations of stellar atmospheres. We present a theoretical derivation as a proof of concept for our method. We perform realistic spectral line formation calculations to quantify the ratio between luminosity and radial velocity amplitude for two case studies: the Sun and the red giant ϵ Tau. Luminosity amplitudes are computed based on the bolometric flux predicted by 3D simulations with granulation background modelled the same way as asteroseismic observations. Radial velocity amplitudes are determined from the wavelength shift of synthesized spectral lines with methods closely resembling those used in Birmingham Solar Oscillations Network (BiSON) and Stellar Oscillations Network Group (SONG) observations. Consequently, the theoretical luminosity to radial velocity amplitude ratios are directly comparable with corresponding observations. For the Sun, we predict theoretical ratios of 21.0 and 23.7 ppm [m s-1]-1 from BiSON and SONG, respectively, in good agreement with observations 19.1 and 21.6 ppm [m s-1]-1. For ϵ Tau, we predict K2 and SONG ratios of 48.4 ppm [m s-1]-1, again in good agreement with observations 42.2 ppm [m s-1]-1, and much improved over the result from conventional empirical scaling relations that give 23.2 ppm [m s-1]-1. This study thus opens the path towards a quantitative understanding of solar-like oscillations, via detailed modelling of 3D stellar atmospheres.

3D NLTE spectral line formation of lithium in late-type stars.

Accurately known stellar lithium abundances may be used to shed light on a variety of astrophysical phenomena such as big bang nucleosynthesis, radial migration, ages of stars and stellar clusters, and planet engulfment events. We present a grid of synthetic lithium spectra that are computed in non-local thermodynamic equilibrium (NLTE) across the stagger grid of three-dimensional (3D) hydrodynamic stellar atmosphere models. This grid covers three Li lines at 610.4, 670.8, and 812.6 nm for stellar parameters representative of FGK-type dwarfs and giants, spanning T eff = 4000-7000 K, log g = 1.5-5.0, [Formula: see text]-0.5, and A(Li) = -0.5-4.0. We find that our abundance corrections are up to 0.15 dex more negative than in previous work, due to a previously overlooked NLTE effect of blocking of UV lithium lines by background opacities, which has important implications for a wide range of science cases. We derive a new 3D NLTE solar abundance of A(Li) = 0.96 ± 0.05, which is 0.09 dex lower than the commonly used value. We make our grids of synthetic spectra and abundance corrections publicly available through the breidablik package. This package includes methods for accurately interpolating our grid to arbitrary stellar parameters through methods based on Kriging (Gaussian process regression) for line profiles, and multilayer perceptrons (a class of fully connected feedforward neural networks) for NLTE corrections and 3D NLTE abundances from equivalent widths, achieving interpolation errors of the order of 0.01 dex.

[Change of ion precipitation and surface roughness of three dental alloys coexisting].

PURPOSE: To study the change of ion precipitation and surface roughness of three dental alloys'coexisting in standard electrolyte solution, in order to provide a reference for the selection of clinical alloy materials. METHODS: Standard samples of Ni-Cr alloy, Co-Cr alloy and Au-Ag-Pd alloy were prepared and divided into 5 groups: Ni-Cr alloy(group N), Co-Cr alloy(group C), Au-Ag-Pd alloy(Group A), Ni-Cr alloy contact with Au-Ag-Pd alloy(group NA), Ni-Cr alloy contact with Co-Cr alloy(group NC). All groups of alloys were soaked in standard electrolyte solution (T=37 ℃, pH=2.31) for 7 days. Inductively coupled plasma mass spectrometry (ICP-MS) was used to measure the amount of ions released from each group. The surface morphology of each group was observed and the surface roughness(Ra) was measured using atomic force microscope（AFM）. SPSS 18.0 software package was used for statistical analysis. RESULTS: The amount of nickel ions released from group N, NA, NC was (1.32±0.03) µg/cm2, (2.13±0.07) µg/cm2, (1.53±0.08) µg/cm2, respectively. Nickel ions of group NA and NC was significantly more than that of group N(P<0.05), nickel ions of group NA was significantly more than that of group NC(P<0.05). The amount of chromium ions released from group N, NA, NC was (0.06±0.01) µg/cm2, (0.08±0.01) µg/cm2, (0.05±0.01) µg/cm2, respectively, the amount of chromium ions of group NA was significantly more than that of group NC(P<0.05). The surface roughness of Ni-Cr alloy in group N, NA, NC was (4.60±0.16) nm, (5.37±0.08) nm, (5.04±0.15) nm, respectively. The surface roughness of Ni-Cr alloy in group NA and group NC was significantly larger than that in group N (P<0.05). When contact with Au-Ag-Pd alloy or Co-Cr alloy, the amount of nickel ions released and the surface roughness of Ni-Cr alloy both significantly increased. Compared with Co-Cr alloy, Au-Ag-Pd alloy caused Ni-Cr alloy to release more nickel, chromium ions and formed a rougher surface.

Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments.

Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein, we report a strategy to fabricate cell-laden constructs with tunable 3D microenvironments achieved by bioprinting of gelatin methacryloyl (GelMA)/alginate core/sheath microfibers, where the alginate sheath serves as a template to support and confine the GelMA pre-hydrogel in the core during the extrusion process, allowing for subsequent UV crosslinking. This novel strategy minimizes the bioprinting requirements for the core bioink, and facilitates the fabrication of cell-laden GelMA constructs at low concentrations. We first showed the capability of generating various alginate hollow microfibrous constructs using a coaxial nozzle setup, and verified the diffusibility and perfusability of the bioprinted hollow structures that are important for the tissue engineering applications. More importantly, the hollow alginate microfibers were then used as templates for generating cell-laden GelMA constructs with soft microenvironments, by using GelMA pre-hydrogel as the bioink for the core phase during bioprinting. As such, GelMA constructs at extremely low concentrations (<2.0%) could be extruded to effectively support cellular activities including proliferation and spreading for various cell types. We believe that our strategy is likely to provide broad opportunities in bioprinting of 3D constructs with cell-favorable microenvironments for applications in tissue engineering and pharmaceutical screening.

Extrusion Bioprinting of Shear-Thinning Gelatin Methacryloyl Bioinks.

Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly in the case of extrusion-based bioprinting. Herein, this paper reports a novel strategy to directly bioprint cell-laden gelatin methacryloyl (GelMA) constructs using bioinks of GelMA physical gels (GPGs) achieved through a simple cooling process. Attributed to their shear-thinning and self-healing properties, the GPG bioinks can retain the shape and form integral structures after deposition, allowing for subsequent UV crosslinking for permanent stabilization. This paper shows the structural fidelity by bioprinting various 3D structures that are typically challenging to fabricate using conventional bioinks under extrusion modes. Moreover, the use of the GPG bioinks enables direct bioprinting of highly porous and soft constructs at relatively low concentrations (down to 3%) of GelMA. It is also demonstrated that the bioprinted constructs not only permit cell survival but also enhance cell proliferation as well as spreading at lower concentrations of the GPG bioinks. It is believed that such a strategy of bioprinting will provide many opportunities in convenient fabrication of 3D cell-laden constructs for applications in tissue engineering, regenerative medicine, and pharmaceutical screening.

Catalytic decomposition of toxic chemicals over metal-promoted carbon nanotubes.

Effective decomposition of toxic gaseous compounds is important for pollution control at many chemical manufacturing plants. This study explores catalytic decomposition of phosphine (PH(3)) using novel metal-promoted carbon nanotubes (CNTs). The cerium-promoted Co/CNTs catalysts (CoCe/CNTs) are synthesized by means of coimpregnation method and reduced by three different methods (H(2), KBH(4), NaH(2)PO(2)·H(2)O/KBH(4)). The morphology, structure, and composition of the catalysts are characterized using a number of analytical instrumentations including high-resolution transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, BET surface area measurement, and inductively coupled plasma. The activity of the catalysts in PH(3) decomposition reaction is measured and correlated with their surface and structural properties. The characterization results show that the CoCe/CNTs catalyst reduced by H(2) possesses small particles and is shown thermally stable in PH(3) decomposition reaction. The activities of these catalysts are compared and are shown in the following sequence: CoCe/CNTs > Co/CNTs > CoCeBP/CNTs> CoCeB/CNTs. The difference in reduction method results in the formation of different active phases during the PH(3) decomposition reaction. After a catalytic activity test, only the CoP phase is formed on CoCe/CNTs and Co/CNTs catalysts, whereas multiphases CoP, Co(2)P, and Co phases are formed on CoCeBP/CNTs and CoCeB/CNTs. Results show that the CoP phase is formed predominantly on the CoCe/CNTs and Co/CNTs catalysts and is found to likely be the most active phase for this reaction. Furthermore, the CoCe/CNTs catalyst exhibits not only highest activity but also long-term stability in PH(3) decomposition reaction. When operated in a fixed-bed reactor at 360 °C, single-pass PH(3) conversion of about 99.8% can be achieved.