ABSTRACT
Objective: To explore the reentry rate of reactive blood donors in the bloodborne pathogen infection screening in Hangzhou City, and analyze the donation behavior of those who successfully returned. Methods: A retrospective analysis of the return data of blood donors with reactive bloodborne pathogen screening markers was conducted at Zhejiang Provincial Blood Center from June 2017 to May 2022. The reentry process for blood donors with reactive bloodborne pathogen screening markers in Hangzhou City is as follows: after the initial screening period of 6 months, donors can voluntarily apply for return to the blood center. Samples are collected and subjected to routine enzyme-linked immunosorbent assay (ELISA) screening for HBsAg, anti-HCV, HIV Ab/Ag, and anti-TP, as well as a single nucleic acid (HIV/HCV/HBV) test. For samples that show non-reactivity in both ELISA and nucleic acid tests, serum biomarker testing for the reasons of exclusion is performed using chemiluminescence immunoassay (CLIA), and those with non-reactivity are allowed to return. Results: A total of 4 583 reactive blood donors who met the criteria for re-entry applied for reentry, out of which 475 applications were received from donors in the Hangzhou area. Among these, 279 donors were successfully readmitted, resulting in a success rate of 58.74% (279/475). By the end of December 2021, out of the 174 donors who successfully returned, 114 donors chose to donate again. They collectively donated 39 530 ml of whole blood and 1 147.2 therapeutic doses of platelets. Among these, 21 donors once again showed reactivity for pathogen infection biomarkers, accounting for 18.42% (21/114). Conclusion: The reentry strategy has somewhat mitigated the attrition of blood donors. Nevertheless, there are instances where donors who were successfully readmitted show reactivity once more in the screening for pathogen infection biomarkers.
Subject(s)
HIV Infections , Nucleic Acids , Humans , Blood Donors , Blood-Borne Pathogens , Retrospective Studies , Mass Screening/methods , Biomarkers , Hepatitis B virusABSTRACT
HLA-A*02:625 differs from HLA-A*02:06:01:01 by a single nucleotide substitution at position 806 C>T.
ABSTRACT
A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction-Sanger sequence-based typing (PCR-SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (TâC) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.
Subject(s)
Alleles , Genetic Variation , HLA-B Antigens/genetics , Amino Acid Sequence , Cloning, Molecular , Codon , Exons , HLA-B Antigens/chemistry , Humans , Male , Middle Aged , Sequence Analysis, DNAABSTRACT
HLA-DQB1*06:209 has one nucleotide change from HLA-DQB1*06:01:01 at position 260 G>C in exon 2.
Subject(s)
Asian People , Bone Marrow/physiology , Genotype , HLA-DQ beta-Chains/genetics , Alleles , Bone Marrow Transplantation , China , Histocompatibility Testing , Humans , Polymorphism, Genetic , Sequence Alignment , Sequence Analysis, DNA , Tissue Donors , World Health OrganizationABSTRACT
HLA-B*40:333 differs from HLA-B*40:01:01 by a single nucleotide substitution at position 380 T>C.
Subject(s)
Alleles , HLA-B Antigens/genetics , Histocompatibility Testing/methods , Polymerase Chain Reaction/methods , Base Sequence , Exons/genetics , Genetic Loci , Humans , Sequence AlignmentABSTRACT
HLA-B*40:338N differs from HLA-B*40:01:01 by a single nucleotide substitution at position 843C>A.
Subject(s)
Alleles , Asian People/genetics , HLA-B Antigens/genetics , Leukemia/genetics , Base Sequence , Exons/genetics , Humans , Sequence AlignmentABSTRACT
HLA-B*13:98 differs from HLA-B*13:02:01 by a single nucleotide substitution at position 193 A>G.
Subject(s)
Alleles , Asian People/genetics , HLA-B Antigens/genetics , Base Sequence , Exons/genetics , HumansABSTRACT
HLA-DQB1*03:181 has one nucleotide change from HLA-DQB1*03:05:01 at position 470C>G.
Subject(s)
Alleles , Asian People/genetics , HLA-DQ beta-Chains/genetics , Leukemia/genetics , Base Sequence , Exons/genetics , HumansABSTRACT
HLA-B*27:04:06 differs from HLA-B*27:04:01 by a single-nucleotide substitution at position 396 C > A.
Subject(s)
Alleles , Asian People/genetics , Bone Marrow/metabolism , HLA-B Antigens/genetics , Tissue Donors , Base Sequence , Exons/genetics , HumansABSTRACT
A novel HLA-B*39:01:01-related variant, HLA-B*39:130, has been identified in a normal individual of Han ethnicity in Hunan province, southern China. Following Sanger polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning, phasing and sequencing. Aligned with HLA-B*39:01:01, HLA-B*39:130 has a nonsynonymous thymine substitution at nucleotide position 94 in exon 4, resulting in amino acid change from threonine to isoleucine at codon 214 (ACAâATA) of the mature HLA-BmRNA molecule.
Subject(s)
Alleles , HLA-B Antigens/genetics , Adult , Base Sequence , Cloning, Molecular , Humans , MaleABSTRACT
HLA-DRB1*15:127 has 1 nucleotide change from HLA-DRB1*15:01:01:01 at position 308 C>G.
Subject(s)
Alleles , Bone Marrow , Genotyping Techniques , HLA-DRB1 Chains/genetics , Polymerase Chain Reaction , Tissue Donors , China , HumansABSTRACT
HLA-B*40:01:41 differs from HLA-B*40:01:01 by a single nucleotide substitution at position 195 G>A.
Subject(s)
Alleles , Genotyping Techniques , HLA-B40 Antigen/genetics , Polymerase Chain Reaction , Asian People , Blood Donors , Fetal Blood , HumansABSTRACT
A new MICA allelic variant, MICA*007:07, was identified in an individual of Mongol ethnicity in the Inner Mongolia Autonomous Region, northern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. MICA*007:07 differs from MICA*007:01 by a synonymous mutation from G to A at the 2nd nucleotide position in exon 2. MICA*007:07 was linked to HLA-B*27:05.
Subject(s)
Base Sequence/genetics , HLA-B Antigens/genetics , Histocompatibility Antigens Class I/genetics , Cloning, Molecular , Exons , Female , HLA-B Antigens/immunology , Histocompatibility Antigens Class I/immunology , Humans , Linkage DisequilibriumABSTRACT
HLA-B*27:147 differs from HLA-B*27:04:01 by a single nucleotide substitution at position 523 C>G.
Subject(s)
Alleles , Genotyping Techniques/methods , HLA-B27 Antigen/genetics , Polymerase Chain Reaction/methods , HumansABSTRACT
HLA-B*55:02:09 and HLA-B*55:80 differ from HLA-B*55:02:01 by 1 single nucleotide substitution, respectively.
Subject(s)
Alleles , Exons , HLA-B Antigens/genetics , Polymorphism, Single Nucleotide , Tissue Donors , Amino Acid Substitution , Asian People , Base Sequence , Bone Marrow Transplantation , Codon/chemistry , Gene Expression , Genotype , HLA-B Antigens/immunology , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Protein Isoforms/genetics , Protein Isoforms/immunology , Sequence Alignment , Sequence Analysis, DNAABSTRACT
HLA-B*52:01:27 differs from HLA-B*52:01:01 by a single nucleotide substitution at position 681 C>T.
Subject(s)
Alleles , Exons , HLA-B52 Antigen/genetics , Polymorphism, Single Nucleotide , Asian People , Base Sequence , Codon/chemistry , Gene Expression , Genotype , HLA-B52 Antigen/immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNA , Tissue DonorsABSTRACT
HLA-C*08:128 shows a substitution C to T at position 704 when compared to HLA-C*08:01:01.
Subject(s)
Alleles , Exons , HLA-C Antigens/genetics , Polymorphism, Single Nucleotide , Transplant Recipients , Amino Acid Substitution , Asian People , Base Sequence , Codon/chemistry , Gene Expression , Genotype , HLA-C Antigens/immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Leukemia/genetics , Leukemia/immunology , Leukemia/pathology , Leukemia/therapy , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
HLA-C*01:109N differs from HLA-C*01:02:01 by a single nucleotide substitution at position 683 G > A.
Subject(s)
Alleles , Codon, Nonsense , Exons , HLA-C Antigens/genetics , Leukemia/genetics , Transplant Recipients , Asian People , Base Sequence , Bone Marrow Transplantation , Cloning, Molecular , Gene Expression , Genotype , HLA-C Antigens/immunology , Histocompatibility Testing , Humans , Leukemia/immunology , Leukemia/pathology , Leukemia/therapy , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
HLA-B*13:83 differs from HLA-B*13:01:01 by 2 nucleotide substitutions at positions 103 and 106.
Subject(s)
Alleles , Blood Donors , Exons , HLA-B13 Antigen/genetics , Polymorphism, Single Nucleotide , Amino Acid Substitution , Asian People , Base Sequence , Codon/chemistry , Cord Blood Stem Cell Transplantation , Gene Expression , Genotype , HLA-B13 Antigen/immunology , Histocompatibility Testing , Humans , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNAABSTRACT
HLA-DRB1*08:69 has one nucleotide change from HLA-DRB1*08:03:02 at position 262 G>A.