ABSTRACT
It has been suggested that the immune system is involved in the development of lipoid nephrosis. The aim of the study was to estimate whether an elevated serum concentration of soluble interleukin-2 receptor (sIL-2R) is caused by increased expression of IL-2 receptors on the surface of lymphocytes T (CD3+CD25+). 20 children with lipoid nephrosis and 15 healthy children served as control were evaluated. T cell subpopulations were assayed with flow cytometry, using monoclonal antibodies. The concentration of sIL-2R was determined by enzyme-linked immunosorbent assay (ELISA). The patients with lipoid nephrosis had elevated serum levels of sIL-2R compared with healthy controls. Although the subpopulation of T cell CD3+CD25+ was decreased in nephrotic children. These results could testify against direct contribution of T cell immune response in the pathogenesis of lipoid nephrosis.
Subject(s)
CD3 Complex/blood , Nephrosis, Lipoid/blood , Receptors, Interleukin-2/blood , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Female , Humans , MaleABSTRACT
The investigations were aimed at an evaluation of the health status of mining-and-power engineering school pupils from the viewpoint of classification to work at a strip mine and power station. Special attention was paid to the detection of possible causes of atherosclerosis and coronary heart disease 811 boys aged 14--20 were examined. Their physical development was good. No pathologies in the respiratory tract were found. Hands force was normal. Of six analysed risk factors most frequent were: hypertension and smoking habit. The percentage of pupils affected was increasing with age: arterial hypertension (from 5.5% to 15.7%) and smoking habit (from 6.1% to 43.3%).
