Changes in hardness and surface topography of tissue conditioners submitted to chemical disinfection.

AIM: The aim of this study was to evaluate the longitudinal effect of chemical disinfection on Shore A hardness, surface roughness (Ra) and morphology of two tissue conditioners (Dura Conditioner [DC] and Softone [SO]). METHODS: Twenty-four specimens (2 mm-thick) were made of each material and randomly divided into three groups (N.=8): control (no disinfection), 10 000 ppm chloride solution (sodium hypochlorite) and Corega Tabs solution (peroxide solution). Soaking was performed daily for 15 min, and Shore A hardness and Ra were measured at baseline and 3, 7, 10, and 14 days. Data were analyzed by repeated measures ANOVA and Bonferroni's test (alfa= 0.05). RESULTS: Chemical disinfection for 14 days with sodium hypochlorite and Corega Tabs affected differently the tested materials. Hardness varied from 8 to 20 for DC and from 8 to 23 for SO with significant interaction (P<0.05) between material and disinfection treatment up to day 7. Ra values (in microm) varied from 1.51 to 4.35 for DC and from 2.08 to 4.15 for SO; there was a significant difference between disinfection treatments (P=0.043) but not between materials (P=0.119). Sodium hypochlorite groups displayed smaller Ra values than the control groups, but did not differ from Corega Tabs groups. Scanning electron microscopy showed different pattern of degradation for each material. CONCLUSIONS: The results suggest that the effect of chemical disinfection on degradation of tissue conditioners is material-specific, but hardness is less affected than surface topography. The overall results support the use of the tested materials for up to three days, independently from the disinfection treatment.

Effects of organic solvents on the enzyme activity of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase in calorimetric assays.

In drug discovery programs, dimethyl sulfoxide (DMSO) is a standard solvent widely used in biochemical assays. Despite the extensive use and study of enzymes in the presence of organic solvents, for some enzymes the effect of organic solvent is unknown. Macromolecular targets may be affected by the presence of different solvents in such a way that conformational changes perturb their active site structure accompanied by dramatic variations in activity when performing biochemical screenings. To address this issue, in this work we studied the effects of two organic solvents, DMSO and methanol (MeOH), in the isothermal titration calorimetry (ITC) kinetic assays for the catalyzed reaction of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Trypanosoma cruzi. The solvent effects on T. cruzi GAPDH had not yet been studied. This enzyme was shown here to be affected by the organic solvents content up to 5.0% for MeOH and up to 7.5% for DMSO. The results show that when GAPDH is assayed in the presence of DMSO (5%, v/v) using the ITC experiment, the enzyme exhibits approximately twofold higher activity than that of GAPDH with no cosolvent added. When MeOH (5%, v/v) is the cosolvent, the GAPDH activity is sixfold higher. The favorable effects of the organic solvents on the Michaelis-Menten enzyme-substrate complex formation ensure the consistency of the biological assays, structural integrity of the protein, and reproducibility over the measurement time. The reaction was also kinetically monitored by standard spectrophotometric assays to establish a behavioral performance of T. cruzi GAPDH when used for screening of potential inhibitors.