[Assessment of changes in the parameters of the quality of life of children aged 14-15 years with chronic catarrhal gingivitis after a complex of therapeutic and preventive measures]. / Otsenka izmenenii parametrov kachestva zhizni detei 14­15 let s khronicheskim kataral'nym gingivitom posle realizatsii kompleksa lechebno-profilakticheskikh meropriyatii.

THE PURPOSE OF THE STUDY: To assess changes in the parameters of the quality of life of children aged 14-15 years with chronic catarrhal gingivitis after the implementation of a complex of therapeutic and preventive measures. MATERIALS AND METHODS: The study involved 2 groups of children aged 14-15 years: group 1 - 60 children with chronic catarrhal gingivitis, group 2 - 60 children without signs of periodontal tissue damage. In 1 group of children, a complex of therapeutic and preventive measures was carried out for a month. A CPQ questionnaire was used to assess the quality of life of children. The survey of children of group 1 was conducted before and 1 month after the complex of therapeutic and preventive measures. Children of group 2 were interviewed once at the initial examination. RESULTS: The worst indicators of quality of life were revealed in children aged 14-15 years with chronic catarrhal gingivitis compared with a group of healthy children. The most pronounced effect of periodontal diseases was noted on the sections of the questionnaire devoted to physical discomfort in the oral cavity, functional disorders, and emotional well-being. After carrying out a complex of therapeutic and preventive measures for 1 month, there was an improvement in the quality of life in general and its individual components. At the same time, the scores of answers to most of the questionnaire questions decreased to the level of indicators in healthy children.

Angiogenesis in cardiopulmonary dirofilariosis: does the Wolbachia surface protein have a pro- or anti-angiogenic effect?

Cardiopulmonary dirofilariosis caused by Dirofilaria immitis produces inflammation, blood vessel obstruction and hypoxia, which are required conditions for the beginning of the process of neovascularization. Since D. immitis harbours intracellular symbiotic Wolbachia bacterium, the global understanding of the angiogenic process requires the analysis of the effect of the parasite molecules, but also that of Wolbachia. Canine primary lung microvascular endothelial cells were treated with the recombinant Wolbachia surface protein (rWSP) and the expression of angiogenic factors like Vascular Endothelial Growth Factor-A (VEGF-A), sFlt, membrane Endoglin (mEndoglin) and soluble Endoglin (sEndoglin), as well as the in vitro formation of pseudocapillaries, were measured. The analyses showed a significant increase in the expression of pro-angiogenic VEGF-A and anti-angiogenic sEndoglin, together with a significant decrease in both pro-angiogenic mEndoglin and pseudocapillary formation, compared to untreated controls. Due to the complexity of the angiogenic process and its relationship with other physiological processes like inflammation and fibrinolysis, these results might suggest that rWSP participate in various mechanisms related to each other and its effects might depend either on the balance between them or on the moment of their occurrence.

A possible relationship between Thromboxane B2 and Leukotriene B4 and the encapsulation of Dirofilaria repens worms in human subcutaneous dirofilariasis.

Human subcutaneous dirofilariosis has several clinical presentations. Many cases present as subcutaneous nodules, as a consequence of a local inflammatory reaction that encapsulates and destroys the worms. In addition, there are cases in which migrating worms located in the ocular area remain unencapsulated. In the present work, the levels of two pro-inflammatory eicosanoids, thromboxane B2 (TxB2) and leukotriene B4 (LTB4) are analysed by commercial Enzime-Linked immunosorbent assay (ELISA) in serum samples from 43 individuals, 28 diagnosed as having subcutaneous dirofilariasis presenting a subcutaneous nodule, five diagnosed as having dirofilariasis, in which the worms remained unencapsulated in the periphery of the eye, and ten healthy individuals living in a non-endemic area, used as controls. The worms were surgically removed, identifying Dirofilaria repens as the causative agent in all cases, by Polymerase Chain Reaction (PCR). Individuals with nodules showed significantly higher levels of TxB2 and LTB4 than healthy controls, whereas significant differences in LTB4 levels were observed between individuals with unencapsulated worms and healthy controls. It is speculated that the absence of LTB4 may contribute to the fact that worms remain unencapsulated as a part of immune evasion mechanisms.

[Age-related changes in protein and lipid oxidation in the liver of prematurely aging rats OXYS]. / Vozrastnye izmeneniia okislennosti belkov i lipidov v pecheni prezhdevremenno stareiushchikh krys OXYS.

Age-dependent variations in products of lipid peroxidation products (LPO) and oxidative damage of proteins have been studied in the liver mitochondria and microsomes of premature aging OXYS rats. Characteristics of oxidative processes in OXYS rats were compared with parameters in Wistar rats. Changes in lipid peroxidation products content and oxidative damage of proteins in rat liver mitochondria and microsomes of both strains rats were nonlinear and had opposite direction during the first year of life. The level of protein oxidative damage in OXYS rat liver mitochondria and cytosol was higher than in Wistar at 12 months. The content of primary and end lipid peroxidation products in OXYS rat liver microsomes was significantly decreased in 12 months, but content of conjugated dienes was increased in mitochondria.

Effect of polysaccharides and human plasma lipoproteins on the secretion of cystatin C by peritoneal macrophages from normal and tumor bearing mice.

Intact peritoneal macrophages in vitro secreted the cysteine proteinase inhibitor cystatin C. Polysaccharides stimulated cystatin C secretion: lipopolysaccharide < carboxymethylated beta-D-glucan < sulfoethylated beta-D-glucan. Human plasma low-density- (LDL) and high-density lipoproteins (HDL) are still more potent inducers of cystatin C secretion by macrophages. Peritoneal macrophages from mice with experimental HA-1 hepatoma compared to those from intact mice secreted more cystatin C with maximum polysaccharide-stimulated secretion after 30 min of incubation. LDL and HDL induced cystatin C secretion by tumor macrophages also.

[Activity and induction of CYP2B, CYP2C, and CYP3A in tissues of cyclophosphane-sensitive and resistant neoplasms and the liver of neoplasm-carrying mice]. / Aktivnost' i indutsiruemost' CYP2B, CYP2C i CYP3A v tkani chuvstvitel'nykh i ustoichivykh k deistviiu tsiklofosfamida opukholei i pecheni myshei-opukholenositelei.

The activities of three cytochrome P450 families involved in metabolic transformation of cyclophosphamide (CP) (CYP2B and CYP2C responsible for metabolic activation of CP and CYP3A responsible for inactivation of CP) have been investigated in lymphosarcoma and liver microsomes of tumor-bearing CBA mice. Two strains of mouse lymphosarcoma distinguished by their sensitivity to cytostatic action of CP were used in this study for implantation in mice femur muscle. There was certain relationship between CP resistance of lymphosarcoma and tumor P450s activity. CYP2B, CYP2C and CYP3A activities in the CP sensitive tumor were comparable to those in liver, and CYP2B, CYP2C were induced by phenobarbital and dexamethasone. CYP2B and CYP2C in the CP resistant tumor were inactive and only slightly induced by dexamethasone. CYP3A activity was lower than in LS tumor and unchanged during drug treatment. Implantation of LS and RLS tumor in mice caused different effects on P450 activities. LS insignificantly influenced liver CYP2B, CYP2C and CYP3A activities and their inducibility by phenobarbital and dexamethasone was similar to that obtained in liver of mice without tumor. At the same time, CYP2B and CYP2C activity in liver of RLS-bearing mice were essentially reduced, the activity CYP3A remained unchanged, and inducibility of CYP2B, CYP2C and CYP3A by phenobarbital and dexamethasone was similar to that in liver of mice without tumor. These results prove the role of cytochromes P450 activating CP in formation drug resistant phenotype of mice lymphosarcoma and suggest possibility of overcoming of this resistance using cytochrome P450 inducers.

[Bilirubin as an endogenous intermediary in the activation of CYP1A1 expression upon exposure to ultrasound]. / Bilirubin kak éndogennyi posrednik v aktivatsii ékspressii CYP1A1 pod deistviem ul'trazvuka.

The induction of cytochrome P4501A1 (CYP1A1) enzyme activity reflects the increased ligand-dependent transcriptional activity of the cognate CYP1A1 gene. The list of ligands includes various xenobotics such as polycyclic and halogenated aromatic hydrocarbons. Until recently, similar role for endogenous compounds was unknown. In the present study the ability of the endogenous heme metabolite, bilirubin, to regulate CYP1A1 activity was examined. The following parameters were investigated: expression of CYP1A1 in rat liver at a level mRNA, protein and functional activity under at the experimental rising of blood bilirubin level. The influence of local ultrasound contact treatment of rats hepatic area in vivo (the intensity 0.4 W/cm2 and duration time of 10 minutes) on blood unconjugated bilirubin concentration and parameters of CYP1A1 transcriptional activity was also investigated. The ultrasound contact action on rat hepatic are increased blood unconjugated bilirubin concentration. The rise of bilirubin levels of in rat blood after intravenous administration of bilirubin as well as after ultrasound treatment was accompanied by increased mRNA CYP1A1, protein and functional activity of CYP1A1. The comparison of these data with that of time-dependent changes of parameters of CYP1A1 transcriptional activity under ultrasound action and experimental rising of blood bilirubin level suggest that induces CYP1A1 and may be an intermediate in the activation of CYP1A1 expression under ultrasound action.

[Effectiveness of biological dressings made of porcine skin in the treatment of burns]. / Effektivnost' biologicheskoi poviazki iz svinoi kozhi pri lechenii ozhogov.

The observation and estimation of results of treatment of 145 patients with burns have shown high efficiency of a biological dressing of porcine skin treated with chemical anticeptics (formaldehyde).