ABSTRACT
BACKGROUND: The co-circulation of Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) viruses increased the risk of outbreaks and coinfections among them. Here, we report cases of coinfection in clinical samples from state of Tocantins, Brazil. METHODS: In 2017, the Central Public Health Laboratory (LACEN) received samples of patients who consulted health units with symptoms compatible with arboviral infections. A total of 102 samples were sent to the Retrovirology Laboratory at the Federal University of São Paulo, where they were tested by RT-qPCR to confirm DENV, ZIKV and CHIKV infections and to detect coinfected patients. RESULTS: We identified with CHIKV monoinfection (52), DENV serotypes 1 (28) and serotypes 2 (22). We did not detect ZIKV. Five patients were characterized with coinfection involving CHIKV and DENV serotype 2. CONCLUSIONS: The presence of co-circulating arboviruses increases the chance of coinfection and demonstrates the importance of differential diagnosis and vector control.
Subject(s)
Chikungunya Fever/epidemiology , Coinfection/epidemiology , Dengue/epidemiology , Zika Virus Infection/epidemiology , Adolescent , Adult , Brazil/epidemiology , Chikungunya Fever/blood , Chikungunya Fever/diagnosis , Chikungunya Fever/genetics , Chikungunya virus/isolation & purification , Child , Coinfection/diagnosis , Cross-Sectional Studies , Dengue/blood , Dengue/diagnosis , Dengue/genetics , Dengue Virus/isolation & purification , Female , Humans , Male , Middle Aged , RNA, Viral , Reverse Transcriptase Polymerase Chain Reaction , Serogroup , Young Adult , Zika Virus/isolation & purification , Zika Virus Infection/bloodABSTRACT
We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.