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Evaluation of a new rapid test for carbapenemase detection in carbapenem resistant Enterobacteriaceae.

Martino, Marines Dalla Valle; Koga, Paula Célia Mariko; Pasternak, Jacyr; Doi, André Mario; Ciola, Claudete Silvia; da Silva, Cely Barreto; Massaia, Irineu Francisco Delfino Silva; da Silva, Itacy Gonçalves Siqueira; de Araújo, Maria Rita Elmor.

J Microbiol Methods ; 115: 20-1, 2015 Aug.

Article in English | MEDLINE | ID: mdl-25953145

ABSTRACT

We evaluated a new phenotypic test for carbapenemase detection. A total of 100 Enterobacteriaceae isolates were selected. The test was compared with conventional PCR for bla(KPC) and bla(NDM) detection. We found 100% sensitivity and specificity, suggesting that this test may be a feasible alternative for rapid carbapenemase detection.

Subject(s)

Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , Carbapenems/pharmacology , Enterobacteriaceae/enzymology , Enterobacteriaceae/genetics , Polymerase Chain Reaction/methods , beta-Lactamases/genetics , Enterobacteriaceae/drug effects , Enterobacteriaceae/isolation & purification , Enterobacteriaceae Infections/microbiology , Humans

ABSTRACT

Subject(s)

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