ABSTRACT
This paper describes a phytochemical and pharmacological study with Calophyllum brasiliense leaves, a medicinal plant employed in folk medicine for the treatment of several ailments. Based on spectroscopic evidence, five phenolic compounds were identified as hyperin (hyperoside), amentoflavone, quercetin, gallic acid, and protocatechuic acid. The fractions and some phenolic compounds exhibited significant analgesic activity against the writhing test and in relation to the second phase (inflammatory pain) of the formalin test in mice, suggesting that this plant can be useful for the treatment of dolorous processes.
Subject(s)
Analgesics/therapeutic use , Biflavonoids , Calophyllum/chemistry , Phytotherapy , Plant Extracts/therapeutic use , Plant Leaves/chemistry , Quercetin/analogs & derivatives , Abdominal Pain/chemically induced , Abdominal Pain/drug therapy , Acetates/chemistry , Acetic Acid/toxicity , Analgesics/isolation & purification , Animals , Brazil , Chemical Fractionation , Chromatography, Thin Layer , Drug Evaluation, Preclinical , Flavonoids/isolation & purification , Flavonoids/therapeutic use , Foot , Formaldehyde/toxicity , Gallic Acid/isolation & purification , Gallic Acid/therapeutic use , Hexanes/chemistry , Hydroxybenzoates/isolation & purification , Hydroxybenzoates/therapeutic use , Injections, Intraperitoneal , Male , Methylene Chloride/chemistry , Mice , Nuclear Magnetic Resonance, Biomolecular , Pain/chemically induced , Pain/drug therapy , Phenols/isolation & purification , Phenols/therapeutic use , Plant Extracts/chemistry , Quercetin/isolation & purification , Quercetin/therapeutic use , Solvents/chemistryABSTRACT
This paper describes the isolation, identification and analgesic activity of a new biflavonoid from Rheedia gardneriana leaves, which correspond to I3-naringenin-II8-4'-OMe-eriodictyol (GB-2a-II-4'-OMe) (1), with a methoxyl group in position 4 of ring-II. Its structure was determined by spectroscopic data and confirmed by an alkaline hydrolysis. Its analgesic effect was evaluated in a writhing test and a formalin test in mice. It was found that this compound exhibits potent and dose-related analgesic action in both experimental models, with ID50's values of 4.5 micromol/kg against the writhing test and 8.2 and 6.8 micromol/kg against the first and second phase of the formalin test, respectively. It was several times more potent than some well-known analgesic drugs used as reference.
Subject(s)
Analgesics/chemistry , Biflavonoids , Flavonoids/chemistry , Pain/drug therapy , Plants, Medicinal/chemistry , Acetaminophen/pharmacology , Acetic Acid , Analgesics/isolation & purification , Analgesics/pharmacology , Animals , Aspirin/pharmacology , Dipyrone/pharmacology , Flavanones , Flavonoids/isolation & purification , Flavonoids/pharmacology , Formaldehyde , Indomethacin/pharmacology , Male , Mice , Models, Molecular , Molecular Conformation , Molecular Structure , Pain/chemically induced , Pain/physiopathology , Plant LeavesABSTRACT
We have isolated two phytoconstituents present in the B. forficata leaves, a medicinal plant employed in folk medicine specially for the treatment of diabetes. These compounds were isolated by column chromatography and identified as beta-sitosterol and kaempferol-3,7-dirhamnoside (kaempferitrin) by spectroscopical data and comparison with authentic samples. A comparative study with different parts of the plant indicated that the latter is present only in the leaves, suggesting that it might be useful for a suitable quality control of phytotherapeutics which contain this organ of B. forficata in its composition.