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Discovery of a novel Mycobacterium asiaticum PRA-hsp65 pattern.
da Silva, William Marco Vicente; Duarte, Mayara Henrique; de Carvalho, Luciana Distásio; de Souza Caldas, Paulo Cesar; Campos, Carlos Eduardo Dias; Redner, Paulo; Ramos, Jesus Pais.
Infect Genet Evol ; 76: 104040, 2019 12.
Article in English | MEDLINE | ID: mdl-31533063

ABSTRACT

Twenty-one pulmonary sputum samples from nine Brazilian patients were analyzed by the PRA-hsp65 method for identification of Mycobacterium species and the results were compared by sequencing. We reported a mutation at the position 381, that generates a suppression cutting site in the BstEII enzyme, thus leading to a new PRA-hsp65 pattern for M. asiaticum identification.


Subject(s)
Mycobacterium Infections/microbiology , Mycobacterium/classification , Point Mutation , Brazil , DNA, Bacterial/genetics , Humans , Mycobacterium/genetics , Mycobacterium/isolation & purification , Phylogeny , Polymerase Chain Reaction/methods , Sputum/microbiology
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