Short-term restoration practices change the bacterial community in degraded soil from the Brazilian semiarid.

Land degradation by deforestation adversely impacts soil properties, and long-term restoration practices have been reported to potentially reverse these effects, particularly on soil microorganisms. However, there is limited knowledge regarding the short-term effects of restoration on the soil bacterial community in semiarid areas. This study evaluates the bacterial community in soils experiencing degradation (due to slash-and-burn deforestation) and restoration (utilizing stone cordons and revegetation), in comparison to a native soil in the Brazilian semiarid region. Three areas were selected: (a) under degradation; (b) undergoing short-term restoration; and (c) a native area, and the bacterial community was assessed using 16S rRNA sequencing on soil samples collected during both dry and rainy seasons. The dry and rainy seasons exhibited distinct bacterial patterns, and native sites differed from degraded and restoration sites. Chloroflexi and Proteobacteria phyla exhibited higher prevalence in degraded and restoration sites, respectively, while Acidobacteria and Actinobacteria were more abundant in sites undergoing restoration compared to degraded sites. Microbial connections varied across sites and seasons, with an increase in nodes observed in the native site during the dry season, more edges and positive connections in the restoration site, and a higher occurrence of negative connections in the degradation site during the rainy season. Niche occupancy analysis revealed that degradation favored specialists over generalists, whereas restoration exhibited a higher prevalence of generalists compared to native sites. Specifically, degraded sites showed a higher abundance of specialists in contrast to restoration sites. This study reveals that land degradation impacts the soil bacterial community, leading to differences between native and degraded sites. Restoring the soil over a short period alters the status of the bacterial community in degraded soil, fostering an increase in generalist microbes that contribute to enhanced soil stability.

Selection of forage grasses for cultivation under water-limited conditions using Manhattan distance and TOPSIS.

Extreme weather events, such as severe droughts, pose a threat to the sustainability of beef cattle by limiting the growth and development of forage plants and reducing the available pasture for animals. Thus, the search for forage species that are more tolerant and adapted to soil water deficit conditions is an important strategy to improve food supply. In this study, we propose utilizing the mathematical concept of the Manhattan distance to assess the variations in the morphological variables of tropical forage grasses under water-limited conditions. This study aimed to select genotypes of tropical forage grasses under different water stress levels (moderate or severe) at this distance and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). Nine varieties from five species were examined. Forage grasses were grown in 12-L pots under three soil irrigation regimes [100% pot capacity-PC (well-irrigated control), 60% PC (moderate drought stress), and 25% PC (severe drought stress)] with four replicates. Drought stress treatments were applied for 25 days during the forage grass tillering and stalk elongation phases. After exposure to drought stress, the growth and morphological traits of forage plants were evaluated. The results show that the use of the Manhattan distance combined with TOPSIS helps in the genotypic selection of more stable tropical forage grass varieties when comparing plants exposed to moderate and severe drought conditions in relation to the nonstressful environment (control). The 'ADR 300', 'Pojuca', 'Marandu', and 'Xaraés' varieties show greater stability when grown in a greenhouse and subjected to water stress environments. The selected forage varieties can be used as parents in plant breeding programs, allowing us to obtain new drought-resistant genotypes.

Magnesium supplementation alleviates drought damage during vegetative stage of soybean plants.

Our working hypothesis was that magnesium (Mg) supplementation modulates plant performance under low water availability and improves drought tolerance in soybean genotypes. Plants of Bônus 8579, M8808 and TMG1180 genotypes were grown under field conditions and subjected to three water stress treatments (control, moderate and severe stress) and three Mg levels [0.9 (low), 1.3 (adequate) and 1.7 cmolc dm-³ (supplementation)]. After 28 days of drought imposition, the growth parameters, osmotic potential, relative water content, leaf succulence, Mg content and photosynthetic pigments were assessed. In general, drought drastically decreased the growth in all genotypes, and the reductions were intensified from moderate to severe stress. Under adequate Mg supply, TMG1180 was the most drought-tolerant genotype among the soybean plants, but Mg supplementation did not improve its tolerance. Conversely, although the M8808 genotype displayed inexpressive responses to drought under adequate Mg, the Mg-supplemented plants were found to have surprisingly better growth performance under stress compared to Bônus 8579 and TMG1180, irrespective of drought regime. The improved growth of high Mg-treated M8808-stressed plants correlated with low osmotic potential and increased relative water content, as well as shoot Mg accumulation, resulting in increased photosynthetic pigments and culminating in the highest drought tolerance. The results clearly indicate that Mg supplementation is a potential tool for alleviating water stress in M8808 soybean plants. Our findings suggest that the enhanced Mg-induced plant acclimation resulted from increased water content in plant tissues and strategic regulation of Mg content and photosynthetic pigments.

Soil and foliar Si fertilization alters elemental stoichiometry and increases yield of sugarcane cultivars.

Silicon (Si) fertilization is widely recognized to improve the development of crops, especially in tropical soils and cultivation under dryland management. Herein, our working hypothesis was that Si stoichiometry favors the efficient use of carbon (C), nitrogen (N), and phosphorus (P) in sugarcane plants. Therefore, a field experiment was carried out using a 3 × 3 factorial scheme consisting of three cultivars (RB92579, RB021754 and RB036066) and three forms of Si application (control without Si; sodium silicate spray at 40 mmol L-1 in soil during planting; sodium silicate spray at 40 mmol L-1 on leaves at 75 days after emergence). All Si fertilizations altered the elemental C and P stoichiometry and sugarcane yield, but silicon-induced responses varied depending on sugarcane cultivar and application method. The most prominent impacts were found in the leaf Si-sprayed RB92579 cultivar, with a significant increase of 7.0% (11 Mg ha-1) in stalk yield, 9.0% (12 Mg ha-1) in total recoverable sugar, and 20% (4 Mg ha-1) in sugar yield compared to the Si-without control. In conclusion, our findings clearly show that silicon soil and foliar fertilization alter C:N:P stoichiometry by enhancing the efficiency of carbon and phosphorus utilization, leading to improved sugarcane production and industrial quality.

Domestication of Lima Bean (Phaseolus lunatus) Changes the Microbial Communities in the Rhizosphere.

Plants modulate the soil microbiota and select a specific microbial community in the rhizosphere. However, plant domestication reduces genetic diversity, changes plant physiology, and could have an impact on the associated microbiome assembly. Here, we used 16S rRNA gene sequencing to assess the microbial community in the bulk soil and rhizosphere of wild, semi-domesticated, and domesticated genotypes of lima bean (Phaseolus lunatus), to investigate the effect of plant domestication on microbial community assembly. In general, rhizosphere communities were more diverse than bulk soil, but no differences were found among genotypes. Our results showed that the microbial community's structure was different from wild and semi-domesticated as compared to domesticated genotypes. The community similarity decreased 57.67% from wild to domesticated genotypes. In general, the most abundant phyla were Actinobacteria (21.9%), Proteobacteria (20.7%), Acidobacteria (14%), and Firmicutes (9.7%). Comparing the different genotypes, the analysis showed that Firmicutes (Bacillus) was abundant in the rhizosphere of the wild genotypes, while Acidobacteria dominated semi-domesticated plants, and Proteobacteria (including rhizobia) was enriched in domesticated P. lunatus rhizosphere. The domestication process also affected the microbial community network, in which the complexity of connections decreased from wild to domesticated genotypes in the rhizosphere. Together, our work showed that the domestication of P. lunatus shaped rhizosphere microbial communities from taxonomic to a functional level, changing the abundance of specific microbial groups and decreasing the complexity of interactions among them.

Tolerance and reduction of chromium by bacterial strains.

Bacteria have potential to tolerate and reduce metals. This study evaluated the potential of selected bacterial strains in tolerating and reducing chromium (Cr). Six bacterial strains (Rhizobium miluonense LCC01, LCC04, LCC05, and LCC69; Rhizobium pusense LCC43; and Agrobacterium deltaense LCC50) showed tolerance to Cr(VI) (16 and 32 µg mL-1), reduction potential of Cr(VI) (from 50 to 80%), and efficiency in producing exopolysaccharides. Rhizobium pusense LCC43 exhibited the highest tolerance (128 µg mL-1), reduction potential of Cr(VI) (from 80 to 100%), and efficiency in producing exopolysaccharides. These results suggested that this strain may have the potential to be used in the bioremediation of soils contaminated with Cr(VI).

Understanding the contribution of soybean crop residues inoculated with Bradyrhizobium spp. and not harvested on nitrogen supply in off-season corn cultivars.

Excessive rainfall in the soybean preharvest period can make mechanized crop harvesting technically and economically unfeasible, causing 100% losses in soybean grain yield. An alternative to reduce the economic losses of farmers would be using unharvested soybean crop residues as a source of nitrogen (N) for the subsequent corn crop. However, a question that still needs to be understood is whether the amount of N released from unharvested soybean residues (straw and grains) is sufficient to meet all the nutritional demand for this nutrient in the off-season corn. Therefore, this study investigated the impact of unharvested soybean crop residue persistence on the yield response of off-season corn crop (Zea mays L.) to the application of N fertilizer rates when grown in tropical Cerrado soils of medium and high fertility. Four simple corn hybrids (SYN7G17 TL, 30F53VYHR, B2433PWU, and AG 8700 PRO3) were grown in soils of medium fertility and medium acidity level (UFMS 1) and high fertility and low acidity level (UFMS 2) and fertilized with five of N fertilizer rates (0, 40, 80, 120, and 160 kg ha-1 of N) applied at 30 days after emergence (DAE). Canonical correlation analysis (CCA) was used to investigate the interrelationships between the groups of independent (agricultural production areas, corn cultivars, and N application rates) and dependent (corn agronomic traits) variables. Crop residues remaining on the soil surface from soybeans not harvested and inoculated with Bradyrhizobium spp. can supply most of the nitrogen requirement of off-season corn grown in succession, especially in tropical soils of medium fertility. However, in high-fertility tropical soils, the maximum grain yield potential of off-season corn cultivars can be obtained with the application of mineral N fertilizer in supplement the amount of nitrogen released from unharvested soybean residues. Therefore, the N requirement depends on the corn cultivar and the agricultural production area. However, our results show that when off-season corn is grown on unharvested soybean residues, nitrogen fertilization in topdressing can be dispensed. The agricultural area with high fertility soil (UFMS 2) enhances the grain yield of the off-season corn crop. The corn cultivar AG 8700 PRO3 has a higher thousand-grain mass and high grain yield potential under Brazilian Cerrado conditions.

Genetically related genotypes of cowpea present similar bacterial community in the rhizosphere.

Plant breeding reduces the genetic diversity of plants and could influence the composition, structure, and diversity of the rhizosphere microbiome, selecting more homogeneous and specialized microbes. In this study, we used 16S rRNA sequencing to assess the bacterial community in the rhizosphere of different lines and modern cowpea cultivars, to investigate the effect of cowpea breeding on bacterial community assembly. Thus, two African lines (IT85F-2687 and IT82D-60) and two Brazilian cultivars (BRS-Guariba and BRS-Tumucumaque) of cowpea were assessed to verify if the generation advance and genetic breeding influence the bacterial community in the rhizosphere. No significant differences were found in the structure, richness, and diversity of bacterial community structure between the rhizosphere of the different cowpea genotypes, and only slight differences were found at the OTU level. The complexity of the co-occurrence network decreased from African lines to Brazilian cultivars. Regarding functional prediction, the core functions were significantly altered according to the genotypes. In general, African lines presented a more abundance of groups related to chemoheterotrophy, while the rhizosphere of the modern cultivars decreased functions related to cellulolysis. This study showed that the genetic breeding process affects the dynamics of the rhizosphere community, decreasing the complexity of interaction in one cultivar. As these cowpea genotypes are genetically related, it could suggest a new hypothesis of how genetic breeding of similar genotypes could influence the rhizosphere microbiome.