Toxicity of polystyrene nanoplastics in Ctenopharyngodon idella juveniles: A genotoxic, mutagenic and cytotoxic perspective.

The increased contamination of surface water with plastic waste is proportional to the increased consumption of products that use them as raw material. However, the impact of these residues on aquatic biota remains limited, mainly when it comes to nanoplastics (NPs). Thus, the aim of the current study is to test the hypothesis that the exposure of Ctenopharyngodon idella juveniles to polystyrene nanoplastics (PS NPs) at low concentrations (0.04 ng/L, 34 ng/L and 34 µg/L), for 20 days, leads to DNA damage and has mutagenic and cytotoxic effects on their erythrocytes. Comet assay enabled observing that DNA damage (inferred from the greater tail length, DNA percentage in the tail and Olive tail moment) induced by PS NPs has increased as the pollutant concentrations have increased, as well as that the formation of micronuclei and other nuclear abnormalities was equitable in animals exposed to this pollutant. On the other hand, there were significant changes in erythrocyte shape and size, oxidative stress generation (NO levels, lipid peroxidation, hydrogen peroxide), antioxidant system inhibition (mediated by total hepatic glutathione) and PS NPs accumulation in the liver and brain of animals exposed to higher concentrations of it. Therefore, the current study has confirmed the initial hypothesis and enhanced the knowledge about the genotoxic, mutagenic and cytotoxic potential of PS NPs in freshwater fish at early developmental stage, relating these effects to biochemical changes and significant accumulation of these nanomaterials. Besides, it is a warning about the (eco) toxicological risk represented by these nanopollutants in aquatic environments. CAPSULE: Polystyrene nanoplastics are capable of inducing DNA damage, mutagenic and cytotoxicity changes in fish.

Toxicity and trophic transfer of polyethylene microplastics from Poecilia reticulata to Danio rerio.

The potential transfer of microplastics (MPs) between vertebrates belonging to the same taxonomic group, and the impact of such a transfer on higher trophic levels remains little explored. An experimental food chain with two fish species was installed to test the hypothesis that polyethylene MPs (PE MPs) can accumulate in animals and cause behavioral, mutagenic and cytotoxic changes at upper trophic levels. Poecilia reticulata fry were exposed to MPs for 48 h and, subsequently, offered (as food) to Danio rerio adults for 10 days to simulate an upper level food chain. PE MPs quantification in fry and in different Danio rerio tissues evidenced their accumulation at the two assessed trophic levels. This finding suggested their absorption, adherence and translocation from one organism to another. The accumulation seen in D. rerio directly exposed to MPs was associated with behavioral disorders at upper trophic level. These animals presented behavior suggestive of anti-predatory response deficit when they were confronted with a potential aquatic predator (Geophagus brasiliensis). This finding was inferred through lower school cohesion, shallower school depth and shorter distance from the potential predator. In addition, animals exposed to MPs recorded higher nuclear abnormality rates and changes in the size and shape of erythrocytes and in their nuclei; this outcome has suggested mutagenic and cytotoxic effects, respectively. Based on the current results, MPs are transferred through a food chain that only involves two vertebrates. MPs enter the vertebrates' organs, change their behavior and induce mutagenic and cytotoxic processes in animals, which can cause significant ecological consequences in freshwater ecosystems.

How much are microplastics harmful to the health of amphibians? A study with pristine polyethylene microplastics and Physalaemus cuvieri.

Microplastics (MPs) are critical emerging pollutants found in the environment worldwide; however, its toxicity in aquatic in amphibians, is poorly known. Thus, the aim of the present study is to assess the toxicological potential of polyethylene microplastics (PE MPs) in Physalaemus cuvieri tadpoles. According to the results, tadpoles' exposure to MP PE at concentration 60 mg/L for 7 days led to mutagenic effects, which were evidenced by the increased number of abnormalities observed in nuclear erythrocytes. The small size of erythrocytes and their nuclei area, perimeter, width, length, and radius, as well as the lower nucleus/cytoplasm ratio observed in tadpoles exposed to PE MPs confirmed its cytotoxicity. External morphological changes observed in the animal models included reduced ratio between total length and mouth-cloaca distance, caudal length, ocular area, mouth area, among others. PE MPs increased the number of melanophores in the skin and pigmentation rate in the assessed areas. Finally, PE MPs were found in gills, gastrointestinal tract, liver, muscle tissues of the tail and in the blood, a fact that confirmed MP accumulation by tadpoles. Therefore, the present study pioneering evidenced how MPs can affect the health of amphibians.

How leachates from wasted cigarette butts influence aquatic life? A case study on freshwater mussel Anodontites trapesiali.

There are several reports on the damage smoking causes to human health available in the literature, but little is known about the environmental and biological consequences from inappropriate cigarette butt (CB) disposal in urban and natural environments. The immunotoxic, morphotoxic and mutagenic potential of leachates from cigarette butts (LCB) diluted at environmentally relevant rates (LCB1x: 1.375%; LCB10x: 13.75%) was evaluated in adult representatives of the bivalve species Anodontites trapesialis, which was adopted as model organism. Type II hyalinocytes and granulocytes (phagocytic cells) frequency increased in the hemolymph of subjects exposed to the pollutant for 14 days. Based on this outcome, LCB chemical constituents did not induce immunotoxic effects. The treatments also did not seem to have any impact on the subjects' hemocitary morphometry parameters: diameter, area, perimeter, circularity and nucleus - cytoplasm ratio. However, subjects in groups LCB1x and LCB10x recorded a larger number of hyalinocytes with some nuclear abnormality such as micronucleus, blebbed nucleus, asymmetric constriction nucleus, and nuclear multilobulation and binucleation. The association between these abnormalities and the treatments was confirmed by the Cr, Ni, Pb, Zn, Mn and Na bioaccumulation in tissue samples of the bivalve models exposed to LCB. To the best of our knowledge, this is the first report on LCB mutagenicity in representatives of a freshwater bivalve group. Given the chemical complexity of the addressed pollutants, it is imperative to develop further investigations about the topic.

Analysis of ZnO nanoparticle-induced changes in Oreochromis niloticus behavior as toxicity endpoint.

The toxicity of zinc oxide nanoparticles (ZnO NPs) has been investigated in different animal models. However, concentrations tested in most studies are often much higher than the ones potentially identified in the environment. Therefore, such toxicity limits the application of these studies to evaluate ecotoxicological risks posed by these nanopollutants. Thus, the aim of the current study is to evaluate the impacts of ZnO NPs (at environmentally relevant concentrations - 760 µg/L and 76,000 µg/L, for 72 h) on the behavioral responses of Oreochromis niloticus (Nile tilapia) exposed to it. Results did not evidence harmful effects of NPs on animals' locomotor abilities (evaluated through open-field and light-dark transition tests), or anxiety-predictive behavior. On the other hand, Zn bioaccumulation in the body tissues of the analyzed tilapias was correlated to changes in eating behavior (motivated by ration pellets), as well as to deficits in antipredatory defensive behavior (under individual and collective conditions). Tilapia exposed to ZnO NPs recorded lower avoidance, flight and territorialist behavior rates when they were individually confronted with potential predators (Salminus brasiliensis). However, collectively exposed animals were unable to recognize their predators, as well as to differentiate them from artificial baits ("false predators"). The present study is the first to report biological impacts resulting from the short exposure of fish-group representatives to ZnO NPs. Thus, we believe that it may be relevant to improve the knowledge about ecotoxicological risks posed by these pollutants.