ABSTRACT
The aim of the present study was to isolate and genotype Toxoplasma gondii from pigs slaughtered for human consumption in northeastern Brazil. Indirect immunofluorescence antibody test (IFAT) was used to screen positive pigs. Tissues samples of animals with antibody titers ≥64 were submitted to bioassay in mice. One isolate of T. gondii was obtained, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, using 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1, and APICO), was applied to evaluate the genetic variability. DNA from reference strains was used as a positive control. By means of genetic analysis, genotype ToxoDB #65 was identified, which is considered an atypical strain. This is the first record of genotype #65 in pigs. Thus, further studies in this region are necessary to determine the genetic variability of T. gondii in pigs and possible impact on public health.
Subject(s)
Swine Diseases/parasitology , Toxoplasma/isolation & purification , Toxoplasmosis, Animal/parasitology , Animals , Brazil , Genotype , Humans , Mice , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Sus scrofa , Swine , Toxoplasma/classification , Toxoplasma/geneticsABSTRACT
The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil.
Subject(s)
Actinobacillus Infections/veterinary , Actinobacillus seminis/isolation & purification , Epididymitis/veterinary , Goat Diseases/microbiology , Orchitis/veterinary , Actinobacillus Infections/complications , Actinobacillus Infections/microbiology , Actinobacillus seminis/classification , Actinobacillus seminis/genetics , Animals , Brazil , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Epididymitis/complications , Epididymitis/microbiology , Goats , Male , Orchitis/complications , Orchitis/microbiology , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was detected in 26.0% and Ureaplasma spp. in 12.0% of semen samples.