ABSTRACT
Fungi of the Candida genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as Turnera subulata become attractive to research. The present work aimed to analyze the ethanol extract of Turnera subulata leaves against standard strains of Candida albicans, Candida krusei and Candida tropicalis using broth microdilution techniques. The identification of the compounds in T. subulata leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of C. albicans and C. tropicalis. It is possible that the T. subulata extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors.
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Ethnobotanical studies have contributed significantly in research of plants with therapeutic potential. The aim of the present study was to learn about the use of native medicinal plants cited by the traditional population in "cerradão" (savanna woodland) areas in Northeast Brazil, providing data on therapeutic indications of the species used and their versatility. MATERIALS AND METHODS: Semi-structured interviews were conducted on the basis of a standardized questionnaire designed for key informants selected using the "snowball" technique. The selection of plants species with therapeutic potential was performed on the basis of the relative importance (RI) and informant consensus fator (ICF). RESULTS: A total of 78 species were indicated for 87 therapeutic purposes. Of these, 11 species presented great versatility of use (RI>1), as e.g.including Copaifera langsdorffii Desf., Hybanthus calceolaria (Mart.) Plumel., Heliotropium cf. indicum L., Croton zehntneri, Croton heliotropiifolius, Myracrodruon urundeuva, Stryphnodendro rotundifolium. Medicinal uses could be generalized Pax & K. Hoffm and Hymenaea courbaril L. The therapeutic indications were grouped into 14 use 15 categories with 594 species-category combinations. The largest number of medicinal species was indicated for illnesses or undefined pain, followed by diseases associated with respiratory, digestive and genitourinaryof body systems. The factor informant consensus highlighted the agreement in the use of plants and showed that the, of which Diseases of the Nervous System and, Diseases of the Circulatory System had the greatest agreement 1.0 and 0.87 repectively, Disorders of the Visual Sensory System - Eyes and Respiratory System Disorder showed the highest agreement of use. CONCLUSIONS: Most of the species cited by the key informants are well known scientifically, but it is interesting that some have been studied little or not all with regard to confirming their purported medicinal properties and can contribute substantially to pharmacological and phytochemical investigations in the search for new drugs.
