A study on phytogenotoxicity induced by biogenic amines: cadaverine and putrescine.

Among the compounds present in necro-leachate, a liquid released during the process of decomposition of the human body, are the biogenic amines cadaverine and putrescine. Although some studies on necro-leachate have indicated a potential ecotoxicological and public health risk associated with it, the research on this type of contamination is still rather limited. This study presents information about the phytotoxic and cytogenotoxic potential of cadaverine and putrescine, evaluated separately and within a mixture. Phytotoxicity was evaluated through a germination test, the initial growth of seedlings with Lactuca sativa, and cytogenotoxicity through chromosomal aberration and micronucleus tests with Allium cepa. The L. sativa results showed a phytotoxic effect for the evaluated amines, by reducing root (> 90%) and hypocotyl (> 80%) elongation. The co-exposure of cadaverine and putrescine potentiated cytogenotoxic activity by aneugenic action in the meristematic cells of A. cepa. From this result, it is possible to infer the eco-toxicogenic potential of cadaverine and putrescine. This study not only highlights the importance of the phytotoxic and cytogenotoxic effects of these amines but also emphasizes the urgent need for further investigation into contamination originating from cemetery environments. By evaluating the risks associated with necro-leachate, this research is aimed at informing global efforts to protect ecological and public health.

Phytotoxicity and cytogenotoxicity of composted tannery sludge.

Tannery sludge (TS) contains high levels of organic matter and chemical elements, mainly chromium (Cr). This can increase its toxicity, rendering it unsuitable for application to soil. However, composting has been proposed as an alternative method for detoxifying TS before its addition to soil. Thus, the aim of this study was to evaluate the phytotoxic and cytogenotoxic potential of untreated (TS) and composted (CTS) tannery sludge in solid and solubilized samples. Seed germination and root growth bioassays were performed with Lactuca sativa, while chromosomal aberrations were assessed using the Allium cepa bioassay. In solid samples, the L. sativa bioassay showed that TS adversely affected germination and root growth, while CTS had a negative affect only on root growth. In solubilized samples, only TS showed significant adverse effects on seed germination and root growth. In both solid and solubilized samples, TS and CTS showed cytotoxic, genotoxic, and mutagenic effects on A. cepa. Thus, results demonstrated that the composting of TS does not result in its complete detoxification. For this reason, TS and CTS cannot be recommended for agricultural use, since they may increase the risk of environmental contamination and crop damage.