ABSTRACT
Within the Dutch Acute HCV in HIV Study, a surveillance system was initiated to estimate the incidence of hepatitis C virus (HCV) infections in 2014. Following the Dutch HIV treatment guidelines, HIV-positive men having sex with men (MSM) in 19 participating centers were screened. Ninety-nine acute HCV infections were reported, which resulted in a mean incidence of 11 per 1000 patient-years of follow-up. Unfortunately, the HCV epidemic among Dutch HIV-positive MSM is not coming to a halt.
Subject(s)
Epidemics , HIV Infections/virology , Hepatitis C/epidemiology , Adult , Coinfection/epidemiology , Coinfection/virology , Hepatitis C/virology , Homosexuality, Male , Humans , Incidence , Male , Middle Aged , Netherlands/epidemiology , Population Surveillance , Risk FactorsABSTRACT
Cutaneous hyperpigmentation is a well-known side effect of tetracyclines, but doxycycline-induced cutaneous hyperpigmentation has only been described in one patient with a therapeutic dosage of doxycycline, and in one patient using suprapharmacological doses. We describe four patients with cutaneous hyperpigmentation in previously unaffected skin, and speculate that this was due to treatment with doxycycline in therapeutic doses. After cessation of therapy, the hyperpigmentation diminished in all four patients, illustrating the need for recognition and timely cessation of therapy.
Subject(s)
Doxycycline/adverse effects , Hyperpigmentation/diagnosis , Skin/pathology , Aged , Anti-Bacterial Agents/adverse effects , Humans , Male , Skin/drug effectsABSTRACT
Infection with Coxiella burnetii may lead to life-threatening chronic Q fever endocarditis or vascular infections, which are often difficult to diagnose. The present study aims to investigate whether measurement of in-vitro interferon-gamma (IFN-γ) production, a key cytokine in the immune response against C. burnetii, differentiates chronic from a past cleared infection, and whether measurement of other cytokines would improve the discriminative power. First, C. burnetii-specific IFN-γ production was measured in whole blood of 28 definite chronic Q fever patients and compared with 135 individuals with past Q fever (seropositive controls) and 908 seronegative controls. IFN-γ production was significantly higher in chronic Q fever patients than in controls, but with overlapping values between patients and seropositives. Secondly, the production of a series of other cytokines was measured in a subset of patients and controls, which showed that interleukin (IL)-2 production was significantly lower in patients than in seropositive controls. Subsequently, measuring IL-2 in all patients and all controls with substantial IFN-γ production showed that an IFN-γ/IL-2 ratio >11 had a sensitivity and specificity of 79% and 96%, respectively, to diagnose chronic Q fever. This indicates that a high IFN-γ/IL-2 ratio is highly suggestive for chronic Q fever. In an additional group of 25 individuals with persistent high anti-Coxiella phase I IgG titres without definite chronic infection, all but six showed an IFN-γ/IL-2 ratio <11. In conclusion, these findings hold promise for the often difficult diagnostic work-up of Q fever and the IFN-γ/IL-2 ratio may be used as an additional diagnostic marker.
Subject(s)
Coxiella burnetii/immunology , Interferon-gamma/metabolism , Interleukin-2/metabolism , Leukocytes, Mononuclear/immunology , Q Fever/diagnosis , Q Fever/immunology , Adult , Aged , Aged, 80 and over , Chronic Disease , Female , Humans , Male , Middle Aged , Sensitivity and SpecificityABSTRACT
We report a 58-year-old man with spondylodiscitis by Mycobacterium bovis-BCG 3 years after intravesical BCG treatment, and shortly after a vertebroplasty. Further examination showed a psoas abscess and oedema around an endovascular aortic graft, which had been placed 1 year earlier. Puncture of the psoas abscess also grew M bovis-BCG. The patient recovered with a combination of antituberculous treatment and surgery. With hindsight a mycotic aneurysm had been present at the time of aortic graft placement and spondylodiscitis at the time of vertebroplasty. This case shows that low grade and longstanding infections may occur following intravesical BCG installation.
Subject(s)
Aorta/microbiology , BCG Vaccine/adverse effects , Lumbar Vertebrae/microbiology , Mycobacterium bovis , Tuberculosis, Cardiovascular/microbiology , Tuberculosis, Spinal/microbiology , Administration, Intravesical , Antitubercular Agents/therapeutic use , Aortic Aneurysm/surgery , BCG Vaccine/therapeutic use , Carcinoma/drug therapy , Humans , Male , Middle Aged , Psoas Abscess/drug therapy , Psoas Abscess/microbiology , Tuberculosis, Cardiovascular/drug therapy , Tuberculosis, Spinal/drug therapy , Urinary Bladder Neoplasms/drug therapy , Vascular Grafting/adverse effects , Vertebroplasty/adverse effectsABSTRACT
A review was performed to determine clinical aspects and diagnostic tools for chronic Q fever. We present a Dutch guideline based on literature and clinical experience with chronic Q fever patients in The Netherlands so far. In this guideline diagnosis is categorized as proven, possible or probable chronic infection based on serology, PCR, clinical symptoms, risk factors and diagnostic imaging.
Subject(s)
Q Fever/diagnosis , Clinical Chemistry Tests , Diagnostic Imaging , Humans , Q Fever/metabolism , Q Fever/microbiologySubject(s)
Skin Ulcer/diagnosis , Syphilis, Cutaneous/diagnosis , Treponema pallidum/isolation & purification , Anal Canal , Diagnosis, Differential , Follow-Up Studies , HIV Seropositivity , Homosexuality, Male , Humans , Male , Middle Aged , Netherlands , Penicillin G Benzathine/therapeutic use , Risk Assessment , Syphilis Serodiagnosis , Syphilis, Cutaneous/drug therapy , Treatment Outcome , Treponema pallidum/drug effectsABSTRACT
OBJECTIVE: To study the adherence to local hospital guidelines for antimicrobial prophylaxis in surgery, and explore reasons for non-adherence. METHODS: A prospective, multicentre audit of elective procedures, without prior suspicion of infection, was carried out in 13 Dutch hospitals. By reviewing medical, anaesthetic and nursing records, and medication charts, the prescription of antibiotics was compared with the local hospital guideline on antibiotic choice, duration of prophylaxis, dose, dosing interval and timing of the first dose. RESULTS: Between January 2000 and January 2001, 1763 procedures were studied. Antibiotic choice, duration, dose, dosing interval and timing of the first dose were concordant with the hospital guideline in 92%, 82%, 89%, 43% and 50%, respectively. Overall adherence to all aspects of the guideline, however, was achieved in only 28%. The most important barriers to local guideline adherence were lack of awareness due to ineffective distribution of the most recent version of the guidelines, lack of agreement by surgeons with the local hospital guidelines, and environmental factors, such as organizational constraints in the surgical suite and in the ward. CONCLUSION: This study shows that, although adherence to separate aspects of local hospital guidelines for surgical prophylaxis in the Netherlands is favourable, overall adherence to all parameters is hard to achieve. Adherence to guidelines on dosing interval and timing needs improvement, in particular. To increase the quality of antimicrobial prophylaxis in surgery, effort should be put into developing guidelines acceptable to surgeons, in adequately distributing the guidelines and to facilitating logistics. Audits of surgical prophylaxis may help hospitals identify barriers to guideline adherence.
