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Arch Virol ; 159(4): 801-5, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24142269

ABSTRACT

A new virus was isolated from a lettuce plant grown in an open field in the Netherlands in 2011. This plant was showing conspicuous symptoms that consisted of necrosis and moderate leaf curling. The virus was mechanically transferred to indicator plants, and a total RNA extract of one of these indicator plants was used for next-generation sequencing. Analysis of the sequences that were obtained and further biological studies showed that the virus was related to, but clearly distinct from, viruses belonging to the genus Torradovirus. The name "lettuce necrotic leaf curl virus" (LNLCV) is proposed for this new torradovirus.


Subject(s)
Lactuca/virology , Plant Diseases/virology , Plant Viruses/classification , Plant Viruses/isolation & purification , RNA Viruses/classification , RNA Viruses/isolation & purification , RNA, Viral/genetics , Cluster Analysis , Genome, Viral , Molecular Sequence Data , Netherlands , Phylogeny , Plant Viruses/genetics , RNA Viruses/genetics , Sequence Analysis, DNA , Sequence Homology
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