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Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.
Nielsen, Jeppe L; Schramm, Andreas; Bernhard, Anne E; van den Engh, Gerrit J; Stahl, David A.
Appl Environ Microbiol ; 70(12): 7550-4, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15574959

ABSTRACT

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.


Subject(s)
Bacteroidetes/genetics , Cloning, Molecular/methods , Flow Cytometry/methods , Gene Library , In Situ Hybridization, Fluorescence/methods , RNA, Ribosomal, 16S/genetics , Fresh Water/microbiology , Geologic Sediments/microbiology , Molecular Sequence Data , Phylogeny , Sequence Analysis, DNA , Time Factors
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