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Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 10): 1127-30, 2013 Oct.
Article
in English
| MEDLINE
| ID: mdl-24100564
ABSTRACT
Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10â Å resolution at 100â K using synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a=96.3, b=101.7, c=108.3â Å, α=82.8, ß=68.2, γ=83.0°. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (VM) of 2.38â Å3â Da(-1) and a solvent content of 48.3%.