Gastrointestinal parasites of owned cats in three districts of Central Ethiopia: Prevalence and risk factors.

This present, cross-sectional study investigated gastrointestinal parasites in owned cats was carried out across Bishoftu, Dukem, and Addis Ababa city and towns in Central Ethiopia from February 2022 to April 2023. The primary objectives were to estimate the prevalence and assess the risk factors associated with these parasites. A total of 213 faecal samples were collected and processed using flotation and McMaster techniques. An overall prevalence of 34.7% (74/213) of gastrointestinal parasites was recorded in the examined cats. The cats harboured nematode (14.6%), cestode (12.7%), and protozoan (5.6%) parasites in single (32.9%) and mixed (1.9%) species. Specifically, Toxocara cati, Dipylidium caninum, Taenia spp., Giardia spp., and Physaloptera spp. were the more frequently identified parasites, with prevalences of 9.4%, 8.9%, 3.8%, 2.8%, and 2.8%, respectively. Statistically significant differences (P < 0.05) were observed in the prevalence of gastrointestinal parasites among cats of different sexes, ages, and feeding conditions. Thus, higher prevalence rates were observed in females (49.1%, OR = 2.3), young cats (70%, OR = 4.7), and those consuming uncooked food (40.5%, OR = 3.6). Faecal consistency also showed a statistically significant association (P < 0.05) with the presence of gastrointestinal parasites. However, no significant differences were noted in the prevalence among cats of different housing conditions, seasons, or agro-ecological zones. Cats sharing shelter with families had the highest prevalence of gastrointestinal parasites (52.2%). In conclusion, this study uncovered a notably high occurrence of gastrointestinal parasites in cats in the surveyed regions, emphasizing the imperative of adopting a One Health strategy to address the zoonotic parasites found in cats, which pose significant public health risks.

Person-centered maternity care during childbirth and associated factors at public hospitals in central Ethiopia.

Objectives: Person-centered maternity care during childbirth is crucial for improving maternal and newborn health outcomes. Therefore, this study was aimed at assessing the determinants of person-centered maternity care in Central Ethiopia. Methods: An institutional-based cross-sectional study was conducted in public hospitals in Central Ethiopia from 30 January to 1 March 2023. A systematic random sampling technique was employed to enroll the study participants. Data were collected through face-to-face interviews using a structured questionnaire. After data collection, it was checked for completeness and consistency, then coded and entered into Epi Data version 4.4.2 and exported to SPSS version 26 for analysis. Both bivariate and multivariable logistic regressions were used to identify associated factors. Results: In this study, a total of 565 participants were involved, resulting in a response rate of 98.77%. The respondents mean score for person-centered maternity care was 60.2, with a 95% CI of (59.1, 62.3). No formal education (ß = -2.00, 95% CI: -4.36, -0.69), fewer than four antenatal contacts (ß = -4.3, 95% CI: -5.46, -2.37), being delivered at night (ß = 2.20, 95% CI: 1.56, 6.45), and complications during delivery (ß = -6.00, 95% CI: -9.2, -0.79) were factors significantly associated with lower person-centered maternity care. Conclusion: This study revealed that person-centered maternity care is low compared with other studies. Consequently, it is imperative to prioritize initiatives aimed at enhancing awareness among healthcare providers regarding the benchmarks and classifications of person-centered maternity care. Moreover, efforts should be directed toward fostering improved communication between care providers and clients, along with the implementation of robust monitoring and accountability mechanisms for healthcare workers to prevent instances of mistreatment during labor and childbirth.

Impact of disrespectful maternity care on childbirth complications: a multicentre cross-sectional study in Ethiopia.

BACKGROUND: Globally, disrespectful, and abusive childbirth practices negatively impact women's health, create barriers to accessing health facilities, and contribute to poor birth experiences and adverse outcomes for both mothers and newborns. However, the degree to which disrespectful maternity care is associated with complications during childbirth is poorly understood, particularly in Ethiopia. AIM: To determine the extent to which disrespectful maternity care is associated with maternal and neonatal-related complications in central Ethiopia. METHODS: A multicentre cross-sectional study was conducted in the West Shewa Zone of Oromia, Ethiopia. The sample size was determined using the single population proportion formula. Participants (n = 440) were selected with a simple random sampling technique using computer-generated random numbers. Data were collected through face-to-face interviews with a pretested questionnaire and were entered into Epidata and subsequently exported to STATA version 17 for the final analysis. Analyses included descriptive statistics and binary logistic regression, with a 95% confidence interval (CI) and an odds ratio (OR) of 0.05. Co-founders were controlled by adjusting for maternal sociodemographic characteristics. The primary exposure was disrespectful maternity care; the main outcomes were maternal and neonatal-related complications. RESULTS: Disrespectful maternity care was reported by 344 women (78.2%) [95% CI: 74-82]. Complications were recorded in one-third of mothers (33.4%) and neonates (30%). Disrespectful maternity care was significantly associated with maternal (AOR = 2.22, 95% CI: 1.29, 3.8) and neonatal-related complications (AOR = 2.78, 95% CI: 1.54, 5.04). CONCLUSION: The World Health Organization advocates respectful maternal care during facility-based childbirth to improve the quality of care and outcomes. However, the findings of this study indicated high mistreatment and abuse during childbirth in central Ethiopia and a significant association between such mistreatment and the occurrence of both maternal and neonatal complications during childbirth. Therefore, healthcare professionals ought to prioritise respectful maternity care to achieve improved birth outcomes and alleviate mistreatment and abuse within the healthcare sector.

Clinical characteristics, treatment patterns, and outcomes of hospitalized patients with acute heart failure in central Ethiopia: a retrospective observational study.

BACKGROUND: Acute heart failure is the rapid onset of new or worsening symptoms and signs of heart failure. Despite the increasing burden of heart failure in developing countries like Ethiopia, there is a paucity of comprehensive data regarding the clinical characteristics, treatment patterns, and outcomes of acute heart failure, especially in the selected study area. Therefore, this study aimed to assess the clinical characteristics, treatment patterns, and outcomes of hospitalized patients with acute heart failure at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia. METHODS: This is a retrospective cross-sectional study of 303 acute heart failure patients who were admitted to the medical wards and intensive care unit of Yekatit 12 Hospital Medical College, Addis Ababa, central Ethiopia, from July 1, 2022, to July 1, 2023. A pretested data abstraction format was used for data extraction from electronic medical records, and SPSS version 26 was used for data analysis. Descriptive analysis was used to summarize sociodemographic data, clinical characteristics, treatment patterns, and outcomes of acute heart failure. Bivariate and multivariate logistic regression models were fitted to identify factors associated with in-hospital mortality. The odds ratio (OR) with the corresponding 95% confidence interval (CI) was calculated to show the strength of the association. RESULTS: Of the 303 patients, 51.5% were females, and the mean age was 56.7 years. The most frequent symptom and sign were dyspnea (98.7%) and peripheral edema (79%), respectively. The commonest underlying cause and precipitating factor of acute heart failure were cor pulmonale (22.8%) and pneumonia (35.3%), respectively. The commonest anti-remodeling medications prescribed on discharge were beta-blockers (47.9%), followed by mineralocorticoid receptor antagonists (42.8%) and angiotensin-converting enzyme inhibitors/angiotensin receptor blockers (38.6%), and the least prescribed were sodium-glucose cotransporter 2 inhibitors (8.3%). The in-hospital mortality rate was 8.6%, and the median length of hospital stay was 9 days. Based on the multivariate logistic regression analysis, the most important predictors of in-hospital mortality were systolic blood pressure (SBP) < 115 mmHg (adjusted odds ratio [AOR] = 6.28; 95% CI: 1.99, 19.78), chloride level < 96 mg/dL (AOR = 4.88; 95% CI: 1.30, 18.33), blood urea nitrogen (BUN) > 20 mg/dl (AOR = 5.48; 95% CI: 1.47, 20.49), and presence of dyslipidemia (AOR = 3.73, 95% CI: 1.15, 12.07). CONCLUSIONS: This study has shown that systolic blood pressure (SBP) < 115 mmHg, blood urea nitrogen (BUN) > 20 mg/dL, chloride (Cl) level < 96 mg/dL, and the presence of dyslipidemia were statistically significant factors associated with in-hospital mortality among patients with acute heart failure. Hence, healthcare providers should stratify patients with acute heart failure upon admission based on their risk of in-hospital mortality and address those potential negative prognostic indicators accordingly.

Occurrence, molecular characterization, and antimicrobial susceptibility of sorbitol non-fermenting Escherichia coli in lake water, fish and humans in central Oromia, Ethiopia.

Contaminated lake water and fish can be sources of bacterial pathogens of public health concern, including pathogenic E. coli. Within Ethiopia, specifically, Central Oromia, raw fish consumption is a common practice. Although there are few reports on occurrence of E. coli O157 in fish destined for human consumption and children under five years, information on the transmission pathways of E. coli O157 and other sorbitol non-fermenting (SN-F) E. coli from water-to-fish-to-human, and their virulence factors and antimicrobial resistant determinants along the fish supply chain is lacking. The study aimed to investigate the occurrence, molecular characteristics, and antimicrobial susceptibility of E. coli O157 and other SN-F E. coli strains in fish, lake water and humans in central Oromia, Ethiopia. A total of 750 samples (450 fish samples, 150 water samples, 150 human stool samples) were collected from five lakes and three health facilities. The samples were processed following the standard protocol recommended by European Food Safety Authority and Kirby-Bauer disc diffusion method for detection of the bacteria, and antimicrobial susceptibility tests, respectively. Molecular characterization of presumptive isolates was performed using Whole-Genome Sequencing (WGS) for serotyping, determination of virulence factors, antimicrobial resistance traits, and genetic linkage of the isolates. Overall, 3.9% (29/750) of the samples had SN-F E. coli; of which 6.7% (n = 10), 1.8% (n = 8) and 7.3% (n = 11) were retrieved from water, fish, and diarrheic human patients, respectively. The WGS confirmed that all the isolates were SN-F non-O157: H7 E. coli strains. We reported two new E. coli strains with unknown O-antigen from fish and human samples. All the strains have multiple virulence factors and one or more genes encoding for them. Genetic relatedness was observed among strains from the same sources (water, fish, and humans). Most isolates were resistant to ampicillin (100%), tetracycline (100%), cefotaxime (100%), ceftazidime (100%), meropenem (100%), nalidixic acid (93.1%) and sulfamethoxazole/trimethoprim (79.3%). Majority of the strains were resistant to chloramphenicol (58.6%) and ciprofloxacin (48.3%), while small fraction showed resistance to azithromycin (3.45%). Isolates had an overall MDR profile of 87.5%. Majority, (62.1%; n = 18) of the strains had acquired MDR traits. Genes encoding for mutational resistance and Extended-spectrum beta-lactamases (ESBL) were also detected. In conclusion, our study revealed the occurrence of virulent and MDR SN-F E. coli strains in water, fish, and humans. Although no genetic relatedness was observed among strains from various sources, the genomic clustering among strains from the same sources strongly suggests the potential risk of transmission along the supply chain at the human-fish-environment interface if strict hygienic fish production is not in place. Further robust genetic study of the new strains with unknown O-antigens, and the epidemiology of SN-F E. coli is required to elucidate the molecular profile and public health implications of the pathogens.

Access to essential medicines for diabetes care: availability, price, and affordability in central Ethiopia.

BACKGROUND: Diabetes is a major global public health burden. Effective diabetes management is highly dependent on the availability of affordable and quality-assured essential medicines (EMs) which is a challenge especially in low-and-middle-income countries such as Ethiopia. This study aimed to assess the accessibility of EMs used for diabetes care in central Ethiopia's public and private medicine outlets with respect to availability and affordability parameters. METHODS: A cross-sectional study was conducted in 60 selected public and private medicine outlets in central Ethiopia from January to February 2022 using the World Health Organization/Health Action International (WHO/HAI) standard tool to assess access to EMs. We included EMs that lower glucose, blood pressure, and cholesterol as these are all critical for diabetes care. Availability was determined as the percentage of surveyed outlets per sector in which the selected lowest-priced generic (LPG) and originator brand (OB) products were found. The number of days' wages required by the lowest paid government worker (LPGW) to purchase a one month's supply of medicines was used to measure affordability while median price was determined to assess patient price and price markup difference between public procurement and retail prices. RESULTS: Across all facilities, availability of LPG and OB medicines were 34.6% and 2.5% respectively. Only two glucose-lowering (glibenclamide 5 mg and metformin 500 mg) and two blood pressure-lowering medications (nifedipine 20 mg and hydrochlorothiazide 25 mg) surpassed the WHO's target of 80% availability. The median price based on the least measurable unit of LPG diabetes EMs was 1.6 ETB (0.033 USD) in public and 4.65 ETB (0.095 USD) in private outlets. The cost of one month's supply of diabetes EMs was equivalent to 0.3 to 3.1 days wages in public and 1.0 to 11.0 days wages in private outlets, respectively, for a typical LPGW. Thus, 58.8% and 84.6% of LPG diabetes EMs included in the price analysis were unaffordable in private and public outlets, respectively. CONCLUSIONS: There are big gaps in availability and affordability of EMs used for diabetes in central Ethiopia. Policy makers should work to improve access to diabetes EMs. It is recommended to increase government attention to availing affordable EMs for diabetes care including at the primary healthcare levels which are more accessible to the majority of the population. Similar studies are also recommended to be conducted in different parts of Ethiopia.

Prevalence, Molecular Detection, and Antimicrobial Resistance of Salmonella Isolates from Poultry Farms across Central Ethiopia: A Cross-Sectional Study in Urban and Peri-Urban Areas.

A cross-sectional study was conducted to assess the prevalence, molecular detection, and antimicrobial resistance of Salmonella isolates within 162 poultry farms in selected urban and peri-urban areas of central Ethiopia. A total of 1515 samples, including cloacal swabs (n = 763), fresh fecal droppings (n = 188), litter (n = 188), feed (n = 188), and water (n = 188), were bacteriologically tested. The molecular detection of some culture-positive isolates was performed via polymerase chain reaction (PCR) by targeting spy and sdfl genes for Salmonella Typhimurium and Salmonella Enteritidis, respectively. Risk factors for the occurrence of the bacterial isolates were assessed. Antimicrobial susceptibility testing of PCR-confirmed Salmonella isolates was conducted using 12 antibiotics. In this study, it was observed that 50.6% of the farms were positive for Salmonella. The overall sample-level prevalence of Salmonella was 14.4%. Among the analyzed risk factors, the type of production, breed, and sample type demonstrated a statistically significant association (p < 0.05) with the bacteriological prevalence of Salmonella. The PCR test disclosed that 45.5% (15/33) and 23.3% (10/43) of the isolates were positive for genes of Salmonella Typhimurium and Salmonella Enteritidis, respectively. The antimicrobial susceptibility test disclosed multi-drug resistance to ten of the tested antibiotics that belong to different classes. Substantial isolation of Salmonella Typhimurium and Salmonella Enteritidis in poultry and on poultry farms, along with the existence of multi-drug resistant isolates, poses an alarming risk of zoonotic and food safety issues. Hence, routine flock testing, farm surveillance, biosecurity intervention, stringent antimicrobial use regulations, and policy support for the sector are highly needed.

Analysis of food security among eucalyptus tree farming smallholder farmers in Gurage Zone, Ethiopia: An application of a composite food security indicator.

This study examines the contribution of eucalyptus tree expansion on rural households' food security status, focusing on the specific context of Ethiopia. Eucalyptus trees pose a significant challenge to the rural food system, warranting investigation. A composite food security indicator was used, and data were collected through household surveys, Focus Group Discussions (FGDs), and Key Informant Interviews (KIIs). Descriptive analysis and multinomial logistic regression models were employed for data analysis. The findings reveal that among the sampled households, 31.2% were classified as food secured, 24.8% as intermediate food secured, and 44.0% as food insecured. Econometric estimations highlight the positive influence of variables such as total land holding and livestock on the likelihood of being in the food secured category. Moreover, a unit increase in income earned from the sale of eucalyptus trees leads to an 8.5% higher probability of being in the intermediate category, while decreasing the likelihood of falling into the categories of food insecurity by 8.1% and food security by 0.4%. Importantly, this study uncovers the diverse consequences of eucalyptus trees across different food security categories, suggesting that the planting of eucalyptus trees for improving rural livelihoods and food security must be tailored to specific household conditions. The research outcomes provide valuable insights for guiding future policies, practices, and research endeavors aimed at achieving a sustainable food system in rural Ethiopia.

Patient Satisfaction with Antiretroviral Therapy Services in Hadiya Zone, Central Ethiopia Using the Donebidean Model: A Time-Motion Study.

Background: A time-motion study is a scientific method for recording time spent on various tasks in a narrow range of specialized work settings, beginning with initial enrollment in ART provision. Therefore, the study aimed to assess the time motion of patient satisfaction with antiretroviral therapy services in Central Ethiopia. Methods: A facility-based cross-sectional study was conducted on a sample of 422 patients from June 14 to July 30, 2021. We used a simple random sampling technique to select the participants. Structural input-related qualitative data were collected using an in-depth interview and used for concurrent triangulation with quantitative. Quantitative data were collected using a standardized and pre-tested questionnaire and analyzed using SPSS version 24.0. Bivariate and multivariable logistic regressions were used to identify independent predictors of time motion and patient satisfaction. The degree of association between the outcome and independent variables was assessed by using an odds ratio with a 95% CI. Results: The time motion of patient satisfaction study found that 53.1% (224/422) of the study participants were satisfied. As independent predictors, time spent (time motion) waiting to be seen by a health professional (AOR = 0.228, 95% CI = 0.079-0.661), patient-provider interaction (AOR = 3.72, 95% CI = 2.111-5.771), perceived privacy (AOR = 2.912, 95% CI = 1.76-2.78), sex (AOR = 2.499, 95% CI = 1.556-4.009), and income class (AOR = 0.228, 95% CI = 0.073-0.707) were associated with outcome variable. Conclusion: The study found low patient satisfaction with ART services, indicating the need for further improvement to enhance patient-centered services with the given time motion. Therefore, further research is needed to assess the intensity and reach of the information through an analysis of pre- and post-intervention that provides a complete picture of conceptualizations of time motion studies.

Molecular and Serological Investigation of Infectious Bronchitis Virus in the East Shewa, Central Ethiopia.

Background: Infectious bronchitis (IB) is an economically important disease in poultry with worldwide distribution. The occurrence of IB has been reported both in commercial and backyard poultry in Ethiopia, although comprehensive information lacks available prevalence of the disease and the circulating serotypes. Methods: A cross-sectional study was conducted from November 2021 to June 2022 in seven commercial farms found in East Shewa, Central Ethiopia. Serological assay using indirect ELISA, virus isolation techniques in embryonated eggs, and molecular techniques such as one-step reverse transcriptase polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (PCR) targeting a 466 bp S1 gene were employed. Results: A total of 196 blood samples, 7 pools (35) of swab samples, and 5 pools of tracheal samples were investigated. The results of serological analysis revealed that 97.96% (192/196; 95% CI: 94.86-99.44) of the sera samples were found to be positive for antibodies against IBV. Out of the 7 pools of swab and 5 pools of tracheal tissue samples analyzed using RT-PCR 33.3% (4/12) of them gave positive results all from swab samples. The RT-PCR-positive samples were subjected to a nested PCR yielding 295bp and 154bp indicating the circulation of Mass and 793/B (4/91) strains of IBV, respectively. The 12 pools of samples inoculated into embryonated egg showed cytopathic changes such as congestion, bleeding, and deformation only after three passages. Conclusion: Two serotypes of IBV are circulating in Ethiopian chickens, and molecular identification of the Massachusetts serotype is the first report in Ethiopia. Further epidemiological investigation is needed in order to devise effective control measures.

Quantitative Assessment of Major Biosecurity Challenges of Poultry Production in Central Ethiopia.

The present study aims to assess the level of implementation of biosecurity practices of small-, medium- and large-scale poultry farms in central Ethiopia. A cross-sectional study design was implemented involving 226 poultry farms (153 small-, 53 medium- and 20 large-scale farms) in three selected areas of central Ethiopia, including Addis Ababa, Bishoftu and West of Shaggar. The results revealed a very low overall biosecurity score of 43.1% for central Ethiopia compared to the global average score of 64.3%. No significant difference (p > 0.05) in the overall biosecurity practices among the three study sites (36.1% for Addis Ababa, 49% for Bishoftu and 34.9% for West of Shaggar) was observed. Six of the eight external biosecurity components with an overall score of 40.7% as compared to the global average of 64.6% were highly disrupted and scored lower values. With regard to internal biosecurity disease management, cleaning and disinfection practices were found to be 52.6% as opposed to the global average of 64%. The poor biosecurity level among poultry farms of central Ethiopia clearly demonstrates the urgent need for the implementation of appropriate biosecurity practices through the active involvement of all stakeholders to combat the impact of various diseases and boost the productivity of the sector.

Access to Essential Medicines for Diabetes Care: Availability, Price, and Affordability in Central Ethiopia.

Background: Diabetes is a major global public health burden. Effective diabetes management is highly dependent on the availability of affordable and quality-assured essential medicines (EMs) which is a challenge especially in low-and-middle-income countries such as Ethiopia. Methods: A cross-sectional study was conducted in 60 selected public and private medicine outlets in central Ethiopia from January to February 2022 using the World Health Organization/Health Action International (WHO/HAI) standard tool to assess access to EMs. We included EMs that lower glucose, blood pressure, and cholesterol as these are all critical for diabetes care. Availability was determined as the percentage of surveyed outlets per sector in which the selected lowest-priced generic (LPG) and originator brand (OB) products were found. The number of days' wages required by the lowest paid government worker (LPGW) to purchase a one month's supply of medicines was used to measure affordability while median price was determined to assess patient price and price markup difference between public procurement and retail prices. Results: Across all facilities, availability of LPG and OB medicines were 34.6% and 2.5% respectively. Only two glucose-lowering (glibenclamide 5mg, metformin 500mg) and two blood pressure-lowering medications (nifedipine 20mg and hydrochlorothiazide 25mg) surpassed the WHO's target of 80% availability. The median price based on the least measurable unit of LPG diabetes EMs was 1.6 ETB (0.033 USD) in public and 4.65 ETB (0.095 USD) in private outlets, respectively. The cost of one month's supply of diabetes EMs was equivalent to 0.3 to 3.1 days wages in public and 1.0 to 11.0 days wages in private outlets, respectively, for a typical LPGW. Thus, 58.8% and 84.6% of LPG diabetes EMs included in the price analysis were unaffordable in private and public outlets, respectively. Conclusion: There are big gaps in availability and affordability of EMs used for diabetes in central Ethiopia. Relevant stakeholders should work to improve access to EMs.

Ethnobotanical study on medicinal plant knowledge among three ethnic groups in peri-urban areas of south-central Ethiopia.

BACKGROUND: Documenting traditional knowledge on plant use among ethnic groups has enabled researchers to obtain a better understanding of how indigenous flora is seen and used in daily life. Their therapeutic applications will also encourage future conservation and phytochemical research, potentially leading to the development of novel drugs. However, past ethnobotanical studies conducted in Ethiopia mainly focused on rural areas, and limited coverage to document the ethnobotanical knowledge at the rural‒urban interface. Therefore, this study was conducted to document and analyze traditional ethnobotanical knowledge on medicinal plants among three selected ethnic groups in peri-urban areas of south-central Ethiopia. In addition, we attempted to investigate the range of cultural similarity and disparity between the studied ethnic groups in relation to traditional medicinal plants and diseases treated. METHODS: Data were collected using semistructured questionnaires and in-depth interviews of 189 key informants, floristic species inventories, and field observations. Several cultural importance indices and Rahman's similarity indices were applied to analyze the relevance of medicinal plants and cultural similarity among the ethnic groups. RESULTS: A total of 189 therapeutic plants representing 159 genera and 69 families were identified and documented across the three studied ethnic groups. Of these, the Sidama, Gedeo, and Oromo ethnic groups reported 28, 34, and 38%, respectively. Most medicinal plants were represented by herbs (36%), followed by shrubs (31%), trees (27%), and herbaceous climbers (7%). Rahman's similarity index (RSI) revealed considerable ethnobotanical knowledge variation among ethnic groups. Oromo and Sidama showed the highest disparity (63.8%), followed by Gedeo and Oromo (63.2%). Of the total collected therapeutic plants, 78 most important medicinal plants were selected for the cultural importance analysis, which revealed that Croton macrostachyus Hochst. ex Delile scored the highest point in the Gedeo and Oromo ethnic groups and Zingiber officinale Roscoe in the Sidama ethnic group. Whereas Cinnamomum verum J.Presl, Psidium guajava L., and Melia azedarach L. are the least. CONCLUSION: The present study revealed the presence of cultural differences in medicinal plant knowledge practices and therapeutic plant use among the studied ethnic groups in rural-urban interface areas of south-central Ethiopia. The diverse healing potential of plants would support future pharmacological investigations, emphasizing the need for adequate documentation of indigenous knowledge and versatile flora to prevent their further loss.

Zoonotic tuberculosis in a high bovine tuberculosis burden area of Ethiopia.

Background: Tuberculosis (TB) is a major cause of ill health and one of the leading causes of death worldwide, caused by species of the Mycobacterium tuberculosis complex (MTBC), with Mycobacterium tuberculosis being the dominant pathogen in humans and Mycobacterium bovis in cattle. Zoonotic transmission of TB (zTB) to humans is frequent particularly where TB prevalence is high in cattle. In this study, we explored the prevalence of zTB in central Ethiopia, an area highly affected by bovine TB (bTB) in cattle. Method: A convenient sample of 385 patients with pulmonary tuberculosis (PTB, N = 287) and tuberculous lymphadenitis (TBLN, N = 98) were included in this cross-sectional study in central Ethiopia. Sputum and fine needle aspirate (FNA) samples were obtained from patients with PTB and TBLN, respectively, and cultures were performed using BACTEC™ MGIT™ 960. All culture positive samples were subjected to quantitative PCR (qPCR) assays, targeting IS1081, RD9 and RD4 genomic regions for detection of MTBC, M. tuberculosis and M. bovis, respectively. Results: Two hundred and fifty-five out of 385 sampled patients were culture positive and all were isolates identified as MTBC by being positive for the IS1081 assay. Among them, 249 (97.6%) samples had also a positive RD9 result (intact RD9 locus) and were consequently classified as M. tuberculosis. The remaining six (2.4%) isolates were RD4 deficient and thereby classified as M. bovis. Five out of these six M. bovis strains originated from PTB patients whereas one was isolated from a TBLN patient. Occupational risk and the widespread consumption of raw animal products were identified as potential sources of M. bovis infection in humans, and the isolation of M. bovis from PTB patients suggests the possibility of human-to-human transmission, particularly in patients with no known contact history with animals. Conclusion: The detected proportion of culture positive cases of 2.4% being M. bovis from this region was higher zTB rate than previously reported for the general population of Ethiopia. Patients with M. bovis infection are more likely to get less efficient TB treatment because M. bovis is inherently resistant to pyrazinamide. MTBC species identification should be performed where M. bovis is common in cattle, especially in patients who have a history of recurrence or treatment failure.

Epidemiological study and dairy farmers' knowledge, attitudes, and practices on foot and mouth disease in central Ethiopia.

Foot and mouth disease (FMD) is consistently ranked as the most economically significant viral disease and one of the top five livestock diseases in Ethiopia. Although FMD is endemic in Ethiopia, the epidemiology and the farmers' knowledge, attitudes, and practices regarding FMD were poorly quantified. Thus, a cross-sectional study was conducted from November 2021 to April 2022 to estimate the seroprevalence, identify the FMD serotypes, and assess the farmers' knowledge, attitudes, and practices on FMD in Addis Ababa city and Sebeta special zone, central Ethiopia. A total of 384 serum samples were collected from cattle and tested using a 3ABC enzyme-linked immunosorbent assay (ELISA). In this study, an overall 56% seroprevalence was recorded. Two types of FMD serotypes were detected in which serotype O was the dominant serotype (75.5%) followed by serotype A (45.5%). A significantly higher seroprevalence (P = 0.00) was recorded in Addis Ababa (85%) compared to Sebeta (28.7%). Seropositivity in older and semi-intensively managed cattle was 2.9 (95% CI: 1.36-6.50; P = 0.006) and 2.1 (95% CI: 1.34-3.26; P = 0.001) times higher compared to young and intensively managed cattle, respectively. A survey on knowledge, attitude, and practice of 103 farmers revealed that 90.2% knew of FMD and the majority of them can recognize its clinical pictures. However, 12.7% of farmers who knew FMD didn't practice any prevention methods. Additionally, 70% of the farmers responded that their cattle roamed outside of their farms for communal grazing, watering, breeding purposes, and vaccination which might put them more at risk of FMD. The current study demonstrated that the majority of farmers have gaps in biosecurity practices and vaccination of cattle against FMD. Therefore, educating farmers on FMD prevention measures is necessary for successful disease control programs.

Household food insecurity and hunger status in Debre Berhan town, Central Ethiopia: Community-based cross-sectional study.

Background: Food security occurs when all people have physical, social, and economic access to sufficient, safe, and nutritious food that fits their dietary needs and food preferences for an active and healthy life at all times. There is limited evidence on this topic and not well studied in Ethiopia. Objective: This study aimed to investigate food insecurity and hunger status among households (HHs) in Debre Berhan town, Ethiopia. Methods: A community-based cross-sectional study was undertaken from 1 January 2017 to 30 January 2017. A simple random sampling technique was used to enroll 395 HHs for the study. An interviewer-administered, structured, and pretested questionnaire was used to collect data through a face-to-face interview. The household food security and hunger status were assessed by using the Household Food Insecurity Access Scale and the Household Hunger Scale, respectively. Data were entered and cleaned using Epiata 3.1 and exported to SPSS software version 20 for statistical analysis. Logistic regression was fitted, and an odds ratio with a 95% confidence interval (CI) and a value of p of less than 0.05 were used to identify factors associated with food insecurity. Results: A total of 377 HHs participated in the study with a response rate of 95.4%. The proportion of households with food insecurity was 32.4%, among which mild, moderate, and severe food insecurity accounted for 10.3, 18.8, and 3.2%, respectively. The mean score of the Household Food Insecurity Access Scale was 1.88 ± 3.5. Hunger occurred among 3.2% of households. The mean score of the Household Hunger Scale was 2.17 ± 1.03. Husband or male cohabitant's occupation (AOR = 2.68; 95% CI: 1.31-5.48) and wife or female cohabitant's literacy (AOR = 3.10; 95% CI: 1.01- 9.55) were the only factors associated with HH food insecurity. Conclusion: HH food insecurity and hunger in Debre Berhan town were unacceptably high, which can hamper achieving national targets for food security, nutrition, and health. Intensified efforts are further needed to accelerate the decline in food insecurity and hunger prevalence. Therefore, interventions need to target self-employed merchants in small businesses and women who are uneducated.

Early initiation of antenatal care and its associated factors among antenatal care followers at public health facilities in Ambo town administration, Central Ethiopia.

Background: Mothers who attend antenatal care (ANC) late miss the opportunity to receive health information, early interventions, and timely referrals, which leads to problems complicating pregnancy and contributes to maternal mortality. Objective: This study was conducted to assess early initiation of ANC visit and its associated factors in public health facilities at Ambo town administration, Oromiya regional state, Central Ethiopia, 2018. Methods: A total sample of 344 pregnant women who attended their first natal care visit were chosen using a systematic random selection procedure for facility-based cross-sectional research. Data were entered into EpiData Version 3.1. "The EpiData Association" Odense, Denmark (In Danish: EpiData foreningen) and analyzed using IBM SPSS Version 21 - International Business Machines Corporation, (Armonk, New York). Descriptive statistics were applied to describe the magnitude of the variable, and binary and multivariable logistic regression analyses were employed to identify factors associated with the early initiation of ANC visits. Statistically significant association was declared at a 95% confidence interval and P value (<0.05). Results: The study revealed that only 30.5% of the study respondents started their first ANC timely. Initiation of early ANC booking was affected by factors like having good knowledge of ANC follow-up (Adjusted odd ratio (AOR) = 2.25, 95% CI: 1.06, 4.78), primigravida (AOR = 2.52, 95% CI: 1.20,5.32), planned pregnancy (AOR = 2.86, 95% CI: 1.05, 7.81), partners whose occupation were merchant (AOR = 3.47, 95% CI: 1.15, 10.46), know the available service on ANC service (AOR = 3.00, 95% CI: 1.31, 6.88), and having advice from health professionals (AOR = 2.27, 95% CI: 1.05, 4.89); all these factors had shown significant association with timing of first ANC. Conclusion: Early initiation of first ANC was relatively low in the study area. Planned pregnancy, number of pregnancies, knowledge of ANC follow-up, partner's occupation, knowing the available service on ANC service, and having advice from health professionals were factors associated with it. To improve the situation, it is important to provide continuous public health education on the importance of early initiation of ANC at public health facilities.

Urban food systems: Factors associated with food insecurity in the urban settings evidence from Dessie and Combolcha cities, north-central Ethiopia.

In Ethiopia, food security and vulnerability assessments have traditionally focused on rural areas. Recently, the issue of urban food security has been attracting attention in global policy debates. However, limited studies are found in the study areas that link food insecurity with major food market chains in the urban food system settings. This study is therefore, aimed at measuring the status of food insecurity in the urban food systems. A mixed study design has been used with randomly selected 506 resident households in Dessie and Combolcha cities, of north-central Ethiopia. We used a pre-tested, semi-structured survey questionnaire to collect quantitative data. The qualitative data has been collected with key informant interview, and focus group discussion among food system actors and facilitators in the selected commodities. We applied Household Food Insecurity Access Scale (HFIAS) to measure the food security status and narration and summarization techniques to analyze the qualitative data. The study confirmed that 33.1% of the respondents were food insecure. Moreover, nine and six individuals per 100 people were forced to 'sleep hungry' and 'not eat in the whole day and night' respectively. Households' food insecurity was significantly associated with the gender of the household head, participation in casual labor, and living house ownership. The degree of food insecurity was deemed to be higher due to weak food system governance, an inconsistent food supply chain, and socio-economic and gender inequalities. However, both formal and informal food systems have their own unique qualities and maximum food security benefit can be achieved if they are well integrated. This implies food security intervention in urban areas needs to refocus their strategies towards systematic and integrated approaches that can promote social security programs like: urban housing, job security, urban agriculture and inclusive food safety net programs. Moreover, multidimensional studies are still important to obtain a wider picture of sustainable urban food security in urban areas and to better integrate the formal and informal food systems.

Magnitude of stunting and its determinant factors among children age 6-59 months at Debre Tabor comprehensive specialized hospital, South Gondar zone, North central Ethiopia, 2020.

Introduction: malnutrition continues to be a significant public health and development concern not only in the developing country but also in the world. It is a serious problem because it is causing the deaths of 3.5 million children under 5 years old per- year. Methods: Institution based cross-sectional study design was employed using sample of 342 children selected through systematic simple random sampling technique from May 1st -July30 /2020. Bivariate and multivariate logistic regression analysis was used. The variables which had significant association were identified on the bases of P value<0.05 and AOR 95% CI. Result: The analysis this study revealed that, 42.6% of children were stunted. The main associated factors of stunting were found to be birth order of the child, maternal occupation, frequency meal per day, mother who did not wash their hand before breastfeeding, (AOR=1.636:95%CI:1.00-2.674), children who were not vitamin A, supplemented (AOR=1.901, 95%CI: 1.162-3.109), and child whose mother were not use family planning (AOR=2.916, 95%CI: 1.064-7.989 were associated with outcome variable. Conclusion and recommendation: From the findings of this study, it is concluded that stunting is still an important problem among children aged 6-59 months. Especial attention should be given on intervention of malnutrition.

Medicinal Plants in Treating Hepatitis B Among Communities of Central Region of Ethiopia.

Purpose: In Ethiopia, most people rely heavily on traditional therapeutic plants that have been used for years. The practice of traditional medicines use to treat hepatitis is currently gaining popularity due to the limited availability and affordability of modern drugs. The aim of this study was, therefore, to assess the traditional medicinal plants use to treat viral hepatitis among communities of Central region of Ethiopia. Methods: Data was collected from November 2018 to December 2021 in Central Ethiopia. An open-ended semi-structured interview was used among purposively selected herbalists, traditional medicine entrepreneurs, village heads, and patients visiting traditional healers for hepatitis treatments. A 5 mL blood sample was collected from patients who visited a traditional healers' clinic for hepatitis treatment and tested for HBsAg and HCV-antibody by using ELISA. Among HBsAg-positives, further nucleic acid test for HBV-DNA load was assessed to measure the effects of prescribed medicinal plants. Results: Herbalists cited 24 plants that were used for hepatitis treatment; of which Rumex nepalensis, Vangueria apiculata, and Solanum incanum were the most frequently cited plants. Remedies were commonly prepared by crushing or powdering, mixing them with water, and taken orally. Forty-two individuals were diagnosed and treated as hepatitis patients by herbalists, of which eight of them were HBsAg-positive but no positives for anti-HCV ELISA. At the third and sixth months of viral load assessment among HBsAg-positive, serum HBV-DNA suppression was observed in three individuals treated with different combinations of frequently cited plants. Conclusion: In this study, traditional healers used various plants to treat hepatitis. HBV-DNA suppressive activity was detected in three NAT-positive individuals who were treated by using a mixture of these frequently cited and highest preference-ranked plants. This suggests that these plants have antiviral properties and serve as a basis for more pharmacological research in the quest for new antiviral agents.