Investigation of electronic, ferroelectric and local electrical conduction behavior of RF sputtered BiFeO3thin films.

Most of the applied research on BiFeO3(BFO) focuses on magnetoelectric and spintronic applications. This calls for a detailed grasp of multiferroic and conduction properties. BFO thin films with (100) epitaxial growth has been deposited on a LaNiO3(LNO) buffered Pt/Ti/SiO2/Si(100) substrate using RF magnetron sputtering. The film formed at 15 mTorr, 570 °C, and with Ar/O24:1 had a reasonably high degree of (100)-preferential orientation, the least surface roughness, and a densely packed structure. We obtained ferroelectric loops with strong polarization (150µC cm-2). The leakage current density is as low as 10-2A cm-2at 100 kV cm-1, implying that space-charge-limited bulk conduction (SCLC) was the primary conduction channel for carriers within BFO films. Local electrical conduction behavior demonstrates that at lower voltages, the grain boundary dominates electrical conduction and is linked to the displacement of oxygen vacancies in the grain boundary under external electric fields. We hope that a deeper understanding of the conduction mechanism will help integrate BFO into viable technologies.

In silicomuscle volume conduction study validatesin vivomeasurement of tongue volume conduction properties using a user tongue array depressor.

Objective.Electrophysiological assessment of the tongue volume conduction properties (VCPs) using our novel multi-electrode user tongue array (UTA) depressor has the promise to serve as a biomarker in patients with bulbar dysfunction. However, whetherin vivodata collected using the UTA depressor accurately reflect the tongue VCPs remains unknown.Approach.To address this question, we performedin silicosimulations of the depressor with an accurate anatomical tongue finite element model (FEM) using healthy human tongue VCP values, namely the conductivity and the relative permittivity, in the sagittal plane (i.e. longitudinal direction) and axial and coronal planes (i.e. transverse directions). We then established the relationship between tongue VCP values simulated from our model to measured human data.Main results.Experimental versus simulated tongue VCP values including their spatial variation were in good agreement with differences well within the variability of the experimental results. Tongue FEM simulations corroborate the feasibility of our UTA depressor in assessing tongue VCPs.Significance.The UTA depressor is a new non-invasive and safe tool to measure tongue VCPs. These electrical properties reflect the tongue's ionic composition and cellular membrane integrity and could serve as a novel electrophysiological biomarker in neurological disorders affecting the tongue.

Electrical impedance myography: A critical review and outlook.

Electrical impedance myography (EIM) technology is finding application in neuromuscular disease research as a tool to assess muscle health. Correlations between EIM outcomes, functional, imaging and histological data have been established in a variety of neuromuscular disorders; however, an analytical discussion of EIM is lacking. This review presents an explanation for clinicians and others who are applying EIM and interpreting impedance outcomes. The background of EIM is presented, including the relation between EIM, volume conduction properties, tissue structure, electrode configuration and conductor volume. Also discussed are technical considerations to guide the reader to critically evaluate EIM and understand its limitations and strengths.

A Bioimpedance-Based Device to Assess the Volume Conduction Properties of the Tongue in Neurological Disorders Affecting Bulbar function.

Goal: Current instruments for bulbar assessment exhibit technical limitations that hinder the execution of clinical studies. The volume conduction properties (VCP) of the tongue reflect ionic content and myofiber integrity and they can serve as a new biomarker for evaluating neurological disorders with bulbar dysfunction. Methods: We designed a standalone bioimpedance measurement system that enables accurate, multi-frequency measurement of tongue anisotropic VCP including conductivity and relative permittivity. The system includes a tongue depressor with 16 non-invasive surface sensors for electrical contact with the tongue at directions 0[Formula: see text], 45[Formula: see text], 90[Formula: see text] and 150[Formula: see text]. The depressor is interfaced with the tongue electronic system with Bluetooth connectivity, and a smartphone application. De-identified patient data is sent by email. Results: We first determined the accuracy of the hardware performing phantom measurements mimicking a broad range of tongue values and determined the error to be [Formula: see text]1%. We then validated our new technology measuring a cohort of 7 healthy human subjects under Institutional Review Board approval. Conclusions: None of the subjects who participated suffered discomfort or gag reflexes. The novel technique presented for intra-oral assessment of tongue VCP provides standard, objective and quantitative data potentially sensitive to alterations in tongue internal structure and composition.

In vivo muscle conduction study of the tongue using a multi-electrode tongue depressor.

OBJECTIVE: To test a novel technology for assessment of the volume conduction properties (VCPs) of the tongue. These properties are electrophysiological data that might reflect alterations in patients with tongue involvement. METHODS: Seven healthy individuals were self-measured. The depressor was placed on the surface of the anterior tongue. Directional differences of VCPs were determined with standard descriptive statistics. RESULTS: Conductivity in longitudinal direction was larger than in transverse direction at 16 (p < 0.05), 32 (p < 0.05), 64 (p < 0.01), and 128 kHz (p < 0.01). No differences were found in relative permittivity. The intraclass correlation was 0.778 and 0.771, respectively. CONCLUSIONS: Our technology provides, for the first time, VCPs of the healthy human tongue. SIGNIFICANCE: Tongue VCPs are standard electrophysiological, quantitative and objective data reflecting ionic content and membrane integrity which could find value for diagnostic purposes and treatment monitoring.

Probing flecainide block of INa using human pluripotent stem cell-derived ventricular cardiomyocytes adapted to automated patch-clamping and 2D monolayers.

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are emerging tools for applications such as drug discovery and screening for pro-arrhythmogenicity and cardiotoxicity as leading causes for drug attrition. Understanding the electrophysiology (EP) of hPSC-CMs is essential but conventional manual patch-clamping is highly laborious and low-throughput. Here we adapted hPSC-CMs derived from two human embryonic stem cell (hESC) lines, HES2 and H7, for a 16-channel automated planar-recording approach for single-cell EP characterization. Automated current- and voltage-clamping, with an overall success rate of 55.0 ±â€¯11.3%, indicated that 90% of hPSC-CMs displayed ventricular-like action potential (AP) and the ventricular cardiomyocytes (VCMs) derived from the two hESC lines expressed similar levels of INa, ICaL, Ikr and If and similarly lacked Ito and IK1. These well-characterized hPSC-VCMs could also be readily adapted for automated assays of pro-arrhythmic drug screening. As an example, we showed that flecainide (FLE) induced INa blockade, leftward steady-state inactivation shift, slowed recovery from inactivation in our hPSC-VCMs. Since single-cell EP assay is insufficient to predict drug-induced reentrant arrhythmias, hPSC-VCMs were further reassembled into 2D human ventricular cardiac monolayers (hvCMLs) for multi-cellular electrophysiological assessments. Indeed, FLE significantly slowed the conduction velocity while causing AP prolongation. Our RNA-seq data suggested that cell-cell interaction enhanced the maturity of hPSC-VCMs. Taken collectively, a combinatorial approach using single-cell EP and hvCMLs is needed to comprehensively assess drug-induced arrhythmogenicity.

The influence of P/As substitution in the melilite-like Na2Co(P2-xAsx)O7 (x = 0.40 and 0.93) solid solutions.

To investigate the influence of P/As substitution on structures and electrical properties, e.g. the effect on material densities, two new solid P/As-doped solutions, Na2CoP1.60As0.40O7 (disodium cobalt diphosphorus arsenic heptaoxide) and Na2CoP1.07As0.93O7 (disodium cobalt phosphorus arsenic heptaoxide), with melilite-like structures have been synthesized by solid-state reactions. Their unit-cell parameters are in agreement with Vegard's law. The obtained structural models were investigated by the bond valence sum (BVS) and charge distribution (CHARDI) validation tools and, for the latter, the structures are described as being built on anion-centred polyhedra. The frameworks can be described as layered and formed by {[Co(P,As)2O7]2-}∞ slabs, with alkali cations sandwiched between the layers and with the interlayer spaces increased due to P/As substitution. The BVS model was extended to a preliminary simulation of the sodium conduction properties in the studied structural type and suggests that the most probable sodium conduction pathways are bidimensional, at the (002) planes.