Captain George Blair RAMC: A doctor prisoner of the Japanese in Singapore and Taiwan in the Second World War.

The papers from George Blair's war service as a prisoner of the Japanese in the Second World War are unusually complete. It is a valuable record because it is representative of those young doctors who provided most of the medical care in the camps, and also because the Taiwan camps are not well documented in the literature.

Monitoring of Organochlorine Pesticides (OCP) in Hair Samples of Wild Herbivorous Mammals Living in Remote and Protected Areas of the Far East and Siberia of Russia.

Monitoring wildlife exposure to biological hazards is a critical component of the wildlife risk assessment. In this study 38 hair samples were collected from 8 different species from ten districts of Russian Far East and Siberia and analysed for the presence of organochlorine pesticides (OCP). 50% of the samples were contaminated with - p, p'-DDT, α-HCH and DDD. DDT was the main contaminant found in 13 sample at concentrations range of 14.3 to 369.5 pg/mg hair, mean 91.9 ± 89.7 pg/mg. α-HCH was detected in three samples with the concentrations range 29.9-180.2 pg/mg. The p, p'-DDD was found only in one hair sample of Siberian roe deer from Altai region at 52.6 pg/mg. The exposure level is depended on animals habitat location. The most contaminated region is Terney district which is in the proximity to the borders with China and North Korea where OCP are still in use.

An updated synthesis of the Geophilomorpha (Chilopoda) of Asian Russia.

A comprehensive overview of the state of knowledge on the ChilopodaGeophilomorpha of the Asian part of Russia is presented, based on the critical revision of all published morphological descriptions and all geographical records. Revised diagnoses for all the 38 nominal species so far reported from Asian Russia are given, with comments on their validity. Among them a total of 18 species are recorded only from this region and many of them from a single locality only. The species belong to Geophilidae s. l., (in the genera Arctogeophilus, Geophilus, Pachymerium, and Strigamia), Schendylidae (Escaryus), and Mecistocephalidae (Agnostrup, Arrup, and Tygarrup). At least two species have been introduced, namely Geophilusflavus and Tygarrupjavanicus. The history of studies on the Geophilomorpha in the Asian part of Russia are also summarized.

Study on Syntaxonomic Diversity of Algal Cenoses in Soils of the Russian Far East, Using an Integrative Taxonomic Approach.

Soil is a unique ecosystem with peculiar biodiversity that includes cyanobacteria and algae. Traditionally, cyanobacterial and algal cenoses were described mainly using the dominance approach, rarely based on the Braun-Blanquet method (floristic classification). More importantly, in both cases, the species of cyanobacteria and algae in communities were identified using classical methods (light microscopy) only. In this study, we present results of soil algal cenoses classification using the Braun-Blanquet approach based on species composition data obtained via an integrative approach. Characteristic tables include 19 out of 108 samples collected in the Jewish Autonomous Region, Primorsky Territory, and Sakhalin Region (Iturup Island) in 2018 and in 2020-2021. Twenty-five species of algae from four classes were identified in these sites. We described three new associations of algal communities-Coelastrelletum aeroterrestricae ass. nova, Vischerietum magnae ass. nova, Bracteacoccetum bullati ass. nova. PCA analysis corroborated the results of syntaxonomic analysis and revealed that Coelastrelletum aeroterrestricae inhabit soils with a high value of P; Vischerietum magnae inhabit soils with high value of soil organic carbon (SOC), N, and higher humidity; and Bracteacoccetum bullati inhabit soils with high K values.

Taxonomic diversity and abundance of enchytraeids (Annelida, Clitellata, Enchytraeida) in the Northern Palaearctic. 1. Asian part.

Background: Enchytraeids, or potworms, are tiny oligochaetes that are distributed worldwide in many terrestrial, freshwater and marine ecosystems. Despite their key role in the functioning of ecosystems, the diversity and abundance of Enchytraeidae are rarely studied due to the laborious process of species identification. The present study addresses this gap and sheds some light on the distribution and abundance of enchytraeids in the lands of the Northern Palearctic. The provided dataset constitutes the latest and comprehensive field sampling of enchytraeid assemblages across the Asiatic part of the Northern Palearctic, encompassing an original set of soil samples systematically collected throughout the region from 2019 to 2022. New information: The dataset includes occurrences from 131 georeferenced sites, encompassing 39 species and 7,074 records. This represents the first dataset providing species-specific information about the distribution and abundance of terrestrial enchytraeids across an extensive geographic area covering the Asian sector of the Northern Palaearctic. The compiled dataset is the key for exploring and understanding local and regional enchytraeid diversity. It may also serve as a valuable resource for monitoring and conserving the entire soil biodiversity.

The Mysterious Amurian Grig Paracyphoderris erebeus Storozhenko, 1980 (Orthoptera: Prophalangopsidae): New Data on Its Distribution, Ecology and Biology.

New data on distribution, ecology and biology of the rare extant species Paracyphoderris erebeus of the almost completely ancient family Prophalangopsidae (Orthoptera) are given. This montane species prefers humid areas with relatively low summer temperatures. Habits, mating behaviour and life history of P. erebeus are extremely similar to those of the North American representatives of the genus Cyphoderris. Nowadays, the Amurian grig is known from the Myaochan, Badzhalsky, Dusse-Alin, Bureinsky and Aezop ridges in the Khabarovsk Territory (north of the Amur River) and Bydyr Mountain in the Jewish Autonomous Region of Russia only. The analysis of the predicted distribution of P. erebeus based on the occurrence data reveals that the populations of the species may be more widely distributed over the southern part of the Russian Far East, at least up to 56° N. The ecologo-geographic model of the species distribution over its range is generated using the Maxent 3.4.4 software for the first time. Modelling of the P. erebeus distribution for 2021-2040 and 2041-2060 shows that the position of the species range appears to be relatively stable but a weak decline in the foretold suitability during climate warming may result in a reduction in population sizes and the subsequent fragmentation of the species population system. In this case, the Amurian grig will become a prospective to be included on the IUCN Red List.

A Description of Echinochasmus pseudobeleocephalus n. sp. (Echinochasmidae) Based on Morphological and Molecular Data.

Adult individuals of Echinochasmus pseudobeleocephalus n. sp. were obtained during an experimental study on trematodes' life cycle. An analysis of the morphometric characteristics of the developmental stages and involvement of first intermediate hosts, snails of the genus Boreoelona, in their life cycle, revealed the identity of the obtained trematodes to the European species Echinochasmus beleocephalus previously discovered in the south of the Russian Far East. However, an analysis of molecular data, in particular sequences of the 28S rRNA gene, showed that the Far Eastern trematodes examined do not belong to European E. beleocephalus despite their morphological similarities. An analysis of phylogenetic relationships within the family Echinochasmidae supported the status of E. pseudobeleocephalus n. sp. as an independent species. Our new data confirmed that the individuals attributed to Echinochasmus can be subdivided into two groups on the basis of the number of head-collar spines and the tail length in cercariae on an intergeneric level.

Complete genome sequence of Yersinia pseudotuberculosis strain SP-1303 from lineage 8, associated with Far East scarlet-like fever.

We report the complete genome sequence of Yersinia pseudotuberculosis strain SP-1303, identified as part of lineage 8 and associated with Far East scarlet-like fever. The genome includes the chromosome, the Yersinia-virulence plasmid (pYV) encoding a type III secretion system essential for virulence, the pVM82 plasmid, and two cryptic plasmids.

Past Endemic Izumi Fever or Yersinia pseudotuberculosis Infection Which Sporadically Reappears.

Izumi fever (IF), also known as Far East scarlet-like fever (FESLF), is caused by Yersinia pseudotuberculosis and it has clinical features resembling those of Kawasaki disease (KD). As both diseases are rare in adolescents and young adults, it is challenging to recognize them, thus often leading to a delayed diagnosis. We herein present two cases of IF or FESLF (IF/FESLF). The first case was misdiagnosed as KD, which led to a diagnostic delay. The second case was recognized earlier owing to our experience with the first case. Although cultures were negative in both cases, presumably due to the prior use of antimicrobial agents, our clinical suspicion and a paired serological assay for anti-Yersinia pseudotuberculosis antibodies finally led to a successful diagnosis.

Study of Viral Coinfection of the Ixodes persulcatus Ticks Feeding on Humans in a Natural Focus of the South of the Far East.

The phenomenon of pathogen co-infection detected in a half-fed Ixodes persulcatus tick taken from a human in the south of the Far East was studied. Research was carried out on PEK, Vero, and Vero-E6 cell lines, outbred mice, and chicken embryos using ELISA, PCR, IMFA, plaque formation, and electron microscopy. The tick contained an antigen and a genetic marker of the tick-borne encephalitis virus (TBEV). The patient had post-vaccination antibodies in a titer of 1:200, as a result of which, obviously, an antibody-dependent elimination of TBEV occurred. The tick-borne co-isolate also contained an unknown pathogen (Kiparis-144 virus), which, in our opinion, was a trigger for the activation of chronic infection in suckling white mice. In the laboratory co-isolate, ectromelia virus was present, as evidenced by paw edema during the intradermal infection of mice, characteristic rashes on the chorioallantoic envelope of chicken embryos, and typical plaques on Vero-E6. The Kiparis-144 virus was not pathogenic for white mice and chicken embryos, but it successfully multiplied in the PEK, Vero, and Vero-E6 lines. Viral co-infection was confirmed by electron microscopy. Passaging on mice contributed to an increase in the virulence of the co-isolate, whose titer increased by 10,000 times by the fifth passage, which poses an epidemiological danger.

First Discovery of the North American Leaf-Mining Moth Chrysaster ostensackenella (Lepidoptera: Gracillariidae) in Russia: The Genetic Diversity of a Novel Pest in Invaded vs. Native Range.

Here, we report the first detection of the North American leaf-mining moth Chrysaster ostensackenella (Fitch, 1859) (Lepidoptera: Gracillariidae) on North American black locust Robinia pseudoacacia (Fabaceae) in Primorsky Krai (the Russian Far East) in July 2022. Overall, six moths were reared from the leaf mines and identified based on adult morphology (forewing pattern and male genitalia) and three of them were DNA barcoding. Description of the leaf mines that allowed us to distinguish the damage of Ch. ostensackenella from other gracillariids associated with R. pseudoacacia is provided. The phylogeographic analysis comparing the DNA barcodes from Russia with those from other invaded countries in Europe (Italy) and East Asia (South Korea and Japan) and from the native range (North America) was performed. Intraspecific genetic diversity reached 3.29%. Altogether, 10 haplotypes were revealed among 21 studied specimens in the Holarctic. The detection of one haplotype common for Japan and the USA (North Carolina) suggests that the invasion to East Asia could have happened from the USA directly, rather than through Europe. A shared haplotype defined for Japan and the Russian Far East points at a possible moth species' spread to Primorsky Krai from earlier invaded Hokkaido. Further distribution of Ch. ostensackenella in East Asia and Europe is expected, bearing in mind the wide planting of R. pseudoacacia in these continents. Furthermore, an accidental introduction of the moth to the Southern Hemisphere, where black locust was introduced, is not ruled out.

A new type of tandem repeats in Myotis petax (Chiroptera, Vespertilionidae) mitochondrial control region.

BACKGROUND: Tandem repeats in mitochondrial DNA control region are known to different animal taxa, including bat species of the family Vespertilionidae. The long R1-repeats in the bat ETAS-domain are often presented in a variable copy number and may exhibit both inter-individual and intra-individual sequence diversity. The function of repeats in the control region is still unclear, but it has been shown that repetitive sequences in some animal groups (shrews, cats and sheep) may include parts of ETAS1 and ETAS2 conservative blocks of mitochondrial DNA. METHODS AND RESULTS: Analysis of the control region sequences for 31 Myotis petax specimens allowed the identification of the inter-individual variability and clarification of the composition of the R1-repeats. The copy number of the R1-repeats varies from 4 to 7 in individuals. The specimens examined do not exhibit a size heteroplasmy previously described for Myotis species. The unusual short 30 bp R1-repeats have been detected in M. petax for the first time. The ten specimens from Amur Region and Primorsky Territory have one or two copies of these additional repeats. CONCLUSIONS: It was determined that the R1-repeats in M. petax control region consist of parts of the ETAS1 and ETAS2 blocks. The origin of the additional repeats seems to be related to the 51 bp deletion in the central part of the R1-repeat unit and subsequent duplication. Comparison of repetitive sequences in the control region of closely-related Myotis species identified the occurrence of incomplete repeats also resulting from the short deletions, but distinct from additional repeats of M. petax.

Genome-wide exploration reveals distinctive northern and southern variants of Clonorchis sinensis in the Far East.

Clonorchis sinensis is a carcinogenic liver fluke that causes clonorchiasis-a neglected tropical disease (NTD) affecting ~35 million people worldwide. No vaccine is available, and chemotherapy relies on one anthelmintic, praziquantel. This parasite has a complex life history and is known to infect a range of species of intermediate (freshwater snails and fish) and definitive (piscivorous) hosts. Despite this biological complexity and the impact of this biocarcinogenic pathogen, there has been no previous study of molecular variation in this parasite on a genome-wide scale. Here, we conducted the first extensive nuclear genomic exploration of C. sinensis individuals (n = 152) representing five distinct populations from mainland China, and one from Far East Russia, and revealed marked genetic variation within this species between "northern" and "southern" geographical regions. The discovery of this variation indicates the existence of biologically distinct variants within C. sinensis, which may have distinct epidemiology, pathogenicity and/or chemotherapic responsiveness. The detection of high heterozygosity within C. sinensis specimens suggests that this parasite has developed mechanisms to readily adapt to changing environments and/or host species during its life history/evolution. From an applied perspective, the identification of invariable genes could assist in finding new intervention targets in this parasite, given the major clinical relevance of clonorchiasis. From a technical perspective, the genomic-informatic workflow established herein will be readily applicable to a wide range of other parasites that cause NTDs.

Middle Holocene Siberian genomes reveal highly connected gene pools throughout North Asia.

The peopling history of North Asia remains largely unexplored due to the limited number of ancient genomes analyzed from this region. Here, we report genome-wide data of ten individuals dated to as early as 7,500 years before present from three regions in North Asia, namely Altai-Sayan, Russian Far East, and the Kamchatka Peninsula. Our analysis reveals a previously undescribed Middle Holocene Siberian gene pool in Neolithic Altai-Sayan hunter-gatherers as a genetic mixture between paleo-Siberian and ancient North Eurasian (ANE) ancestries. This distinctive gene pool represents an optimal source for the inferred ANE-related population that contributed to Bronze Age groups from North and Inner Asia, such as Lake Baikal hunter-gatherers, Okunevo-associated pastoralists, and possibly Tarim Basin populations. We find the presence of ancient Northeast Asian (ANA) ancestry-initially described in Neolithic groups from the Russian Far East-in another Neolithic Altai-Sayan individual associated with different cultural features, revealing the spread of ANA ancestry ∼1,500 km further to the west than previously observed. In the Russian Far East, we identify 7,000-year-old individuals that carry Jomon-associated ancestry indicating genetic links with hunter-gatherers in the Japanese archipelago. We also report multiple phases of Native American-related gene flow into northeastern Asia over the past 5,000 years, reaching the Kamchatka Peninsula and central Siberia. Our findings highlight largely interconnected population dynamics throughout North Asia from the Early Holocene onward.

Multilocus genetic analysis indicates taxonomic status of "Candidatus Rickettsia mendelii" as a separate basal group.

To date, the phylogeny of Rickettsia spp. from basal groups is based on the small number of identified species. Thus, the finding of "Candidatus Rickettsia mendelii" in 2016 is of great interest. In this study, "Ca. R. mendelii" was first identified in the Asian region in a new carrier, Ixodes pavlovskyi. "Candidatus R. mendelii", along with "Candidatus Rickettsia tarasevichiae", were found in Ixodes ticks collected on Russky Island (the Far East), where I. pavlovskyi coexists with I. persulcatus. To establish the taxonomic position of "Ca. R. mendelii", a detailed genetic study was carried out. "Candidatus R. mendelii" was genotyped by five genetic fragments (16S rRNA, gltA, and ompB genes, groESL operon, and 23S-5S IGS region); among them, the ompB gene, groESL operon and 23S-5S IGS region were sequenced for the first time. In addition, "Ca. R. tarasevichiae" was genetically characterized by eight genetic loci (16S rRNA, gltA, ompA, ompB, sca4, htrA genes, groESL operon, and 23S-5S IGS region), of which the sca4 gene was first determined. Phylogenetic analysis indicated that regardless of analyzed genetic loci, "Ca. R. mendelii" formed a separate well-supported cluster on each phylogenetic tree. Phylogenetic analysis based on concatenated sequences of gltA, ompB, and groEL gene fragments (total length of 3191 bp) demonstrated that "Ca. R. mendelii", like Rickettsia bellii, is a basal group of Rickettsia.

Isatuximab-Pomalidomide-Dexamethasone Versus Pomalidomide-Dexamethasone in East Asian Patients With Relapsed/Refractory Multiple Myeloma: ICARIA-MM Subgroup Analysis.

BACKGROUND: In the pivotal phase III, randomized, multicenter ICARIA-MM study (NCT02990338), isatuximab plus pomalidomide and dexamethasone (Isa-Pd) improved progression-free survival and overall response rate versus pomalidomide and dexamethasone (Pd) in the overall population of patients with relapsed/refractory multiple myeloma. PATIENTS AND METHODS: In this predefined subgroup analysis, efficacy, and safety between East Asian patients and the overall population were assessed. RESULTS: In total, 36 East Asian patients were included (Japanese, n = 13; Korean, n = 9; Taiwanese, n = 14). At a median follow-up of 11.6 months, median progression-free survival was not reached (95% confidence interval [CI] 5.80-not calculable) in the Isa-Pd arm and was 7.9 months (95% CI 2.90-not calculable) in the Pd arm. The hazard ratio for the between-group difference was 0.52 (95% CI 0.19-1.39), which was similar to the overall population (hazard ratio, 0.60; 95% CI 0.44-0.82). No new safety signals were observed, except that a higher proportion of patients in the East Asian population experienced Grade ≥ 3 neutropenia compared with the overall population. CONCLUSION: These results confirm the efficacy of Isa-Pd in East Asian patients with relapsed/refractory multiple myeloma, and the related safety data are consistent with those observed in the overall population and are manageable.

Influence of vessel upsizing on pollution emissions along Far East-Europe trunk routes.

The emissions of vessels are higher when they sail at sea than when they are at a port, and such emissions affect air quality considerably. In this study, an activity-based method was used to calculate the quantities of various pollutants emitted by large container ships sailing at optimal speed on Far East-Europe trunk routes, which cover seven of the largest economies with high population density. We determined the emission quantities of various pollutant, such as carbon dioxide (CO2), sulfide [Formula: see text]), nitride ([Formula: see text]), and particulate matter-for the newest mega container ships deployed on the aforementioned routes by THE Alliance. This study considered vessel speed as a critical variable and found that to reduce vessel emissions, port authorities should mandate that vessels reduce their speed when entering and leaving a port. Considering maritime practice, setting the optimal speed of a vessel as 15-18 knots is the most effective method for reducing emissions. On the basis of the obtained results, appropriate recommendations are made to the International Maritime Organization, international shipping companies, and port authorities.

Anthropogenic fire patterns affect niche breadth and niche overlap in sympatric songbird species.

The severity of wildfires increases globally, and return intervals decrease. Fires can benefit biodiversity, as post-burn early successional stages provide diverse habitats and niches for many species. How fire disturbance affects niche use and niche overlap of species is poorly understood so far. We studied the effect of anthropogenic fire on breeding habitat use, niche breadth and niche overlap of five sympatric bunting species breeding in wetlands of the Amur River floodplain (Russian Far East). Fire frequency, measured as the time an area burnt in the period 2000 to 2017, was mapped from Landsat imagery and related to the presence or absence of the species. Niche breadth and niche overlap were calculated separately for occurrences in burned (within the study year) and unburned patches. Fire frequency characterized differences in niche use among the species, but the probability of presence was not affected by recent fire in four of five species. Niche breadth was significantly lower in recently burned patches, but we found no increase in niche overlap between species after fire. Instead, the studied species seemed to occupy similar patches before and after fire, possibly because of a high site fidelity. Our results clearly show that fire frequency is a major determinant for the niche separation in the five studied species, while recent fire does not affect niche overlap.

Clostridioides difficile epidemiology in the Middle and the Far East.

OBJECTIVES: Clostridioides difficile is an important pathogen of healthcare-associated gastrointestinal infections. Recently, an increased number of C. difficile infection (CDI) surveillance data has been reported from Asia. The aim of this review is to summarize the data on the prevalence, distribution and molecular epidemiology of CDI in the Middle and the Far East. METHODS: Literature was drawn from a search of PubMed up to September 30, 2021. RESULTS: The meta-analysis of data from 111 studies revealed the pooled CDI prevalence rate in the Middle and the Far East of 12.4% (95% CI 11.4-13.3); 48 studies used PCR for CDI laboratory diagnoses. The predominant types (RT)/sequence type (ST) differ between individual countries (24 studies, 14 countries). Frequently found RTs were 001, 002, 012, 017, 018 and 126; RT017 was predominant in the Far East. The epidemic RT027 was detected in 8 countries (22 studies), but its predominance was reported only in three studies (Israel and Iran). The contamination of vegetable and meat or meat products and/or intestinal carriage of C. difficile in food and companion animals have been reported; the C. difficile RTs/STs identified overlapped with those identified in humans. CONCLUSIONS: A large number of studies on CDI prevalence in humans from the Middle and the Far East have been published; countries with no available data were identified. The number of studies on C. difficile from non-human sources is limited. Comparative genomic studies of isolates from different sources are needed.

The beetles (Insecta, Coleoptera) of the southwest of Primorsky Krai, Russian Far East.

Background: The article is based on the results of the authors' field studies on the fauna of Coleoptera in the southwest of Primorsky Krai, conducted in 1990, 1999, 2015 and 2017-2022. The collection of material was carried out in more than 150 geographical points in the territories of the Khasansky, Nadezhdensky and Ussuriysky raion of the Primorsky Krai of Russia. In addition, small collections materials stored at the Federal Scientific Center of East Asia Terrestrial Biodiversity FEB RAS (Vladivostok, Russia) and Moscow Pedagogical State University (Moscow, Russia) were studied. This is the first generalised list of beetles for south-western Primorye and protected natural areas. A total of 13274 beetles belonging to 629 species from 311 genera and 44 families were studied. In addition to our own collections, the sample includes literature data on 10008 specimens belonging to 355 species from 142 genera and 16 families. New information: This is the first dataset that provides data on the taxonomic composition and geographic distribution with precise coordinates for 47 families of Coleoptera in the southwest of Primorsky Krai, Russia.