Comparative study on mechanical performance of silty soil and expansive soil below canal structures in cold regions.

Silty soil was widely used as filling soil materials for the replacement of expansive soil in cold regions. This paper presents a straightforward approach for the effects of wetting-drying-freezing-thawing cycles on mechanical behaviors of silty soil and expansive soil by laboratory tests. The results showed that the silty soil and expansive soil after 7th wetting-drying-freezing-thawing cycles presented the decreases of elastic modulus, failure strength, cohesion and angel of internal friction by 8.9 %∼12.0 %, 7.7 %∼9.0 %, 7.9 %, 4.5 % and 17.6 %∼37.0 %, 20.5 %∼29.4 %, 43.2 %, 13.0 %, respectively, indicating that wetting-drying-freezing-thawing cycles had little impact on mechanical property of silty soil and a great influence on that of expansive soil. Among them, the mechanical property attenuation ratio in the first three wetting-drying-freezing-thawing cycles accounted for over 90 % of the total. In the meantime, the micro-structure damage, surface crack characteristics and grain size distribution variations of expansive soil were all more significantly than these of silty soil exposed to wetting-drying-freezing-thawing cycles, which brought insight into the causes of the differences in mechanical properties for silty soil and expansive soil. It is found that the silty soil properties were more stable than expansive soil properties, and the silty soil is very effective for replacing the expansive soil below canal structures in cold regions.

Maize residue retention shapes soil microbial communities and co-occurrence networks upon freeze-thawing cycles.

Maize residue retention is an effective agricultural practice for improving soil fertility in black soil region, where suffered from long freezing-thawing periods and intense freeze-thawing (FT) cycles. However, very few studies have examined the influence of maize residue retention on soil microbial communities under FT cycles. We investigated the response of soil microbial communities and co-occurrence networks to maize residue retention at different FT intensities over 12 cycles using a microcosm experiment conditioned in a temperature incubator. Our results indicated that maize residue retention induced dramatic shifts in soil archaeal, bacterial and fungal communities towards copiotroph-dominated communities. Maize residue retention consistently reduced soil fungal richness across all cycles, but this effect was weaker for archaea and bacteria. Normalized stochastic ratio analysis revealed that maize residue retention significantly enhanced the deterministic process of archaeal, bacterial and fungal communities. Although FT intensity significantly impacted soil respiration, it did not induce profound changes in soil microbial diversity and community composition. Co-occurrence network analysis revealed that maize residue retention simplified prokaryotic network, while did not impact fungal network complexity. The network robustness index suggested that maize residue retention enhanced the fungal network stability, but reduced prokaryotic network stability. Moreover, the fungal network in severe FT treatment harbored the most abundant keystone taxa, mainly being cold-adapted fungi. By identifying modules in networks, we observed that prokaryotic Module #1 and fungal Module #3 were enhanced by maize residue retention and contributed greatly to soil quality. Together, our results showed that maize residue retention exerted stronger influence on soil microbial communities and co-occurrence network patterns than FT intensity and highlighted the potential of microbial interactions in improving soil functionality.

Development of a Sampling and Storage Protocol of Extracellular Vesicles (EVs)-Establishment of the First EV Biobank for Polytraumatized Patients.

In the last few years, several studies have emphasized the existence of injury-specific EV "barcodes" that could have significant importance for the precise diagnosis of different organ injuries in polytrauma patients. To expand the research potential of the NTF (network trauma research) biobank of polytraumatized patients, the NTF research group decided to further establish a biobank for EVs. However, until now, the protocols for the isolation, characterization, and storage of EVs for biobank purposes have not been conceptualized. Plasma and serum samples from healthy volunteers (n = 10) were used. Three EV isolation methods of high relevance for the work with patients' samples (ultracentrifugation, size exclusion chromatography, and immune magnetic bead-based isolation) were compared. EVs were quantified using nanoparticle tracking analysis, EV proteins, and miRNAs. The effects of different isolation solutions; the long storage of samples (up to 3 years); and the sensibility of EVs to serial freezing-thawing cycles and different storage conditions (RT, 4/-20/-80 °C, dry ice) were evaluated. The SEC isolation method was considered the most suitable for EV biobanking. We did not find any difference in the quantity of EVs between serum and plasma-EVs. The importance of particle-free PBS as an isolation solution was confirmed. Plasma that has been frozen for a long time can also be used as a source of EVs. Serial freezing-thawing cycles were found to affect the mean size of EVs but not their amount. The storage of EV samples for 5 days on dry ice significantly reduced the EV protein concentration.

Effect of Pleurotus eryngii on the Characteristics of Pork Patties during Freezing and Thawing Cycles.

Temperature fluctuations severely damage the quality, oxidation stability, and structure of pork patties. This study investigated the potential reasons for Pleurotus eryngii (Pe) to protect frozen pork patties from quality degradation caused by temperature fluctuations and promoted the application of a natural ingredient. In this experiment, the pH, the water holding capacity (WHC), the properties of color and texture, the appearance, the degree of protein and lipid oxidation, and the microstructure of patties with different additions of Pe (0%, 0.25%, 0.50%, 1.00%, and 2.00%) were intensified during freezing and thawing (F-T) cycles. The results showed that patties with 0.50% Pe exhibited a distinguishable improvement in the changes of pH, WHC, color, and texture during F-T cycles (p < 0.05). With the times of F-T cycles increasing, 0.50% Pe was able to inhibit lipid oxidation of patties by decreasing the peroxide value (POV) and the thiobarbituric acid reactive substances (TBARS) value to 0.87 and 0.66-fold, respectively, compared to those in the control group. It was also able to suppress the protein oxidation of the patties with a protein sulfhydryl content increasing to 1.13-fold and a carbonyl content decreasing to 0.49-fold compared to the patties in the control group (p < 0.05) after 5 F-T cycles. In addition, the figures of appearance and microstructure of samples indicated that 0.50% Pe effectively restrained the deterioration of structure features from patties after 5 F-T cycles. Thus, the addition of Pe effectively maintained the characteristics of pork patties under F-T cycles.

Remediation of Cu, Cr(VI) and Pb polluted soil with industrial/agricultural by-products in seasonally frozen areas.

Soils contaminated with potentially toxic elements (PTEs) may face serious environmental problems and pose health risks. In this study, the potential feasibility of industrial and agricultural by-products as low-cost green stabilization materials for copper (Cu), chromium (Cr(VI)) and lead (Pb) polluted soil was investigated. The new green compound material SS âˆ¼ BM âˆ¼ PRP was prepared by ball milling with steel slag (SS), bone meal (BM), and phosphate rock powder (PRP) which had an excellent stabilization effect on contaminated soil. Under 20% SS âˆ¼ BM âˆ¼ PRP addition into the soil, the toxicity characteristic leaching concentrations of Cu, Cr(VI) and Pb were reduced by 87.5%, 80.9% and 99.8%, respectively, and the phytoavailability and bioaccessibility of PTEs were reduced by more than 55% and 23%. The freezing-thawing cycle significantly increased the activity of heavy metals, and the particle size became smaller due to the fragmentation of the soil aggregates while SS âˆ¼ BM âˆ¼ PRP could form calcium silicate hydrate by hydrolysis to cement the soil particles, which inhibited the release of PTEs. Different characterizations indicated that the stabilization mechanisms mainly involved ion exchange, precipitation, adsorption and redox reaction. Overall, the results obtained suggest that the SS âˆ¼ BM âˆ¼ PRP is a green, efficient and durable material for remediation of various heavy metal polluted soils in cold regions and a potential method for co-processing and reusing industrial and agricultural wastes.

Influence of Synthetic Limestone Sand on the Frost Resistance of Magnesium Potassium Phosphate Cement Mortar.

Synthetic limestone sand has advantages, such as stable quality and adjustable particle size distribution, and has gradually substituted high-quality natural sand as a fine aggregate in concrete production. The project team has prepared Magnesium Potassium Phosphate Cement (MKPC) mortar by replacing part of the river sand with machine-made limestone sand in equal amounts, which proves that its physical and mechanical properties are obviously better than mortar prepared by whole river sand. However, the research on the impact of machine-made limestone sand on the durability of MKPC mortar has not been carried out. As the repairing material of concrete structures, the frost resistance of MKPC mortar must be evaluated. In this study, the effect of synthetic limestone sand on the frost resistance of Magnesium Potassium Phosphate Cement (MKPC) mortar was investigated by characterizing the strength, mass loss rate, and water absorption of specimens subjected to freeze-thaw cycling. MKPC mortars prepared using solely river sand (M0) or limestone sand (M1) were completely degraded after 225 freezing-thawing cycles in water, whereas the flexural and compressive strengths of MKPC mortar (M2) prepared using both river and synthetic limestone sands was 29.3 and 22.0% of the initial strengths, respectively. The water freeze-thaw resistance of M2 specimens were significantly higher than that of M0 and M1 specimens, and the sulfate freeze-thaw resistance of M1 and M2 were significantly higher than that of M0. The mass loss of MKPC mortar is not more than 0.4% when it is frozen and thawed 225 times in water and 5% Na2SO4 solution, which is far lower than the damage standard of 5%. Based on the favorable composition of the two aggregates, the initial open porosity of M2 was relatively low, owing to the lower water-cement ratio of the mortar at the same flow rate.

Biochar addition regulates soil phosphorus fractions and improves release of available phosphorus under freezing-thawing cycles.

Currently, the shortage of phosphorus resources is becoming more and more serious. In general, phosphorus fertilizer is poorly utilized in soil and tends to gradually accumulate. Freezing-thawing cycles (FT) are seasonal phenomenon occurring in high latitudes and altitudes regions, which have obvious influence on the form of phosphorus in soil. This study investigates the effect of biochar on soil physicochemical properties, phosphorus form and availability under FT and thermostatic incubation (TH) condition. Compared with treatment without biochar, 4 % biochar addition increased the soil pH value, electrical conductivity, organic matter and Olsen-P of soil by a maximum of 0.76, 285.55 µS/cm, 28.60 g/kg and 139.27 mg/kg, respectively. Moreover, according to Hedley-P classification results, under FT condition, the content of labile phosphorus pool is always higher than those under TH. FT may promote the conversion of phosphorus from other fractions to labile phosphorus pool. Redundancy analysis results show that biochar addition and FT can not only directly change the soil phosphorus pool, but also alter the soil physicochemical properties and microbial community, which further affect the adsorption and mineralization of phosphorus in soil. The results of this study will be devoted to understanding the changes in soil phosphorus fractions under the effects of biochar addition and FT, providing references for agricultural production in areas where FT occur.

Formation of advanced glycation end-products in silver carp (Hypophthalmichthys molitrix) surimi products during heat treatment as affected by freezing-thawing cycles.

Lipid oxidation and protein degradation, along with the formation of advanced glycation end-products (AGEs), including Nε-carboxymethyl-lysine (CML), Nε-carboxyethyl-lysine (CEL) and fluorescent AGEs, in raw and subsequently heated surimi products were investigated during freezing-thawing cycles. Lipid oxidation, protein degradation, Schiff base, and AGEs formation constantly increased during freezing-thawing cycles and heat treatment (P < 0.05). Heat-induced increase of AGEs in surimi products was accelerated by freezing-thawing treatment. Formation of CML in one-stage heated (45 °C, 30 min) samples increased from 0.10 ± 0.04 to 0.53 ± 0.11 mg/kg and CEL increased from 0.03 ± 0.32 to 0.92 ± 0.74 mg/kg. In two-stage heated samples (45 °C, 30 min and 90 °C, 20 min), CML increased from 0.86 ± 0.06 to 1.12 ± 0.11 mg/kg and CEL from 1.00 ± 0.34 to 2.11 ± 1.86 mg/kg, during up to 6 freezing-thawing cycles. Formation of fluorescent AGEs derived from heating was also significantly accelerated by freezing-thawing treatment (P < 0.01). Correlation analysis suggested that the chemical synthesis of AGEs in surimi products was promoted by lipid oxidation and protein degradation during freezing-thawing cycles. AGEs formation through Schiff base oxidation likely occurred only under thermal treatment since no relationship was found between Schiff base and AGEs levels in raw surimi products.

Release and transformation of phosphorus in sediment following seasonal freezing-thawing cycles.

River ice in the upper Yarlung Zangbo River is characterized by seasonal freezing-thawing cycles (SFTC). It is important to explore the effects of SFTC on phosphorus release and transformation from upstream surface sediments to protect the ecosystem of the Yarlung Zangbo River. The process and mechanism of phosphorus release and transformation in sediments following SFTC were investigated in a laboratory simulation experiment. The results showed that after freezing, sediment particles were broken, the specific surface area was increased by 14%, and the particle size was decreased by 43%, which resulted in weakened adsorption of phosphorus by sediments. Moreover, the destruction of organic matter (OM) on the sediment surface will release more ion adsorption sites and promote the release of phosphorus. The bioavailabilities of exchangeable phosphorus (Ex-P), aluminum phosphorus (Al-P) and iron phosphorus (Fe-P) increased by 60.09%, 86.86% and 31.86%, respectively, after freezing. Organic phosphorus (O-P) is used indirectly by organisms, and O-P content showed a significant correlation with OM content. Water affected the oxygen content in sediments during the freezing period, and continuous hypoxia promoted the release and transformation of Fe-P and Al-P.

Effect of pre-swelling and freezing/thawing cycles on the structure of molecular, morphological, and functional properties of potato starch.

This study aimed to investigate the effect of pre-swelling at 55°C for 1 hr followed by freezing-thawing cycles (PFTCs), and freezing-thawing cycles (FTCs) in the starch granules to improve the freeze-thaw stability and evaluate its impact on the molecular, morphological, and functional properties of potato starch (PS). FTCs at 1 cycle and 7 cycles were applied for both treated PS. Microscopical structure, thermal, molecular, and functional properties (i.e., swelling power, solubility, shear viscosity, and gel strength) were comprehensively analyzed. In terms of granule structures, treated PS by FTC showed a slightly affected on the surface of starch granules, while treating PS by PFTC showed an affected in the form of small cracks and holes in the outer surface of starch granules. The gelatinization enthalpy (∆Hgel ) values decreased in the treated PS compared with the native. Thus, decreasing was systemically increased with the number of applied cycles from 1- to 7-cycle. The viscosity of treated PS decreased systematically with molecular degradation or the physical modification, with remarkable reduction, particularly at a higher shear rate (150°C). Treated PS by FTC showed a clear difference (p ≤ .05) in gel values compared with the native at disintegration temperature 115°C. Finally, the degradation of the molecular properties showed significant differences between the native and treated PS either by the FTC or PFTC in molecular weight of starch and amylose without debranching and after debranching by pullulanase enzyme. PRACTICAL APPLICATIONS: Freezing is one of the standard preservation methods used for ready-to-eat products. When this type of food's exposed to more freeze-thaw cycles, the phase separation will be increased due to the increase in retrogradation of amylopectin. To avoid such changes during frozen storage, native potato starch (PS) was modified using both pre-swelling followed by freezing-thawing cycles (PFTCs) and freezing-thawing cycles (FTCs) at 1- and 7-cycle to enhance starch properties, such as swelling power, solubility, shear viscosity, and gel strength. The findings of this study might add to the theoretical understanding of modified PS and act as a guideline for modified starch manufacturing.

Effect of multiple freezing/thawing cycles on the physicochemical properties and structural characteristics of starch from wheat flours with different gluten strength.

The physicochemical properties and structural characteristics of starches from three wheat flours with different gluten strength (S-YM20, S-ZM27, and S-ZM366) during freezing/thawing (F/T) cycles were studied. After F/T treatment, the damaged starch content of these three starches all increased, and the lowest increment of damaged starch content after 8 F/T cycles was S-ZM366; the most serious distribution of particle surface concave hole and fracture was S-YM20, followed by S-ZM27 and S-ZM366; additionally, the results of solubility, swelling power, thermal stability and pasting properties indicated S-ZM366 exhibited the strongest resistance to F/T cycles. The differences of freezing resistance among the three starches were possibly ascribed to the differences in compositions, crystallinity and microstructure among these three starches. This study provides theoretical contribution to the development of frozen dough industry from the perspective of wheat variety.

Stability of nivolumab in its original vials after opening and handing in normal saline bag for intravenous infusion.

OBJECTIVES: Nivolumab is an anti-programmed cell death-1 monoclonal antibody, approved for numerous indications in oncohaematological cancers. It is available as solution for infusion at 10 mg/ml. In accordance with the Summary of Product Characteristics (SmPCs), the product is stable for 24 h at 2-8 °C after dilution. However, to anticipate the needs and constraints related to the handling of the product, the aim was to obtain additional information that will contribute to the risk assessment in case of deviation. Potential changes in the stability of Opdivo® leftovers (10 mg/ml) and diluted nivolumab in normal saline solution (2 mg/ml) over a period exceeding 24 h, at different temperatures and after freezing/thawing cycles were studied. METHODS: Turbidimetry, Ultraviolet (UV)-spectroscopy, dynamic light scattering and chromatography were used to evaluate physicochemical stability. Potential pharmacological variations were monitored in vitro by a functional binding inhibition method. RESULTS: No change was detected after 1 month of storage at 2-8 °C neither after 7 days at 40 °C. Although slight changes were detected only after 3 months under 2-8 °C, major changes were found for the same period of time at 40 °C (variants in the distribution profile, slight increase in oligomers and fragments and UV spectral modifications). Physical instability was observed upon 3 freeze/thaw cycles, with the appearance of a new protein population associated with an increase in polydispersity index. CONCLUSION: In conclusion, our results provide additional rationale to the SmPCs, regarding the use of leftovers, reassignment of bags, pre-preparation or breaking the cold chain for Nivolumab.

Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles.

We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.

Brown bear sperm double freezing: Effect of elapsed time and use of PureSperm(®) gradient between freeze-thaw cycles.

The use of sexed spermatozoa has great potential to captive population management in endangered wildlife. The problem is that the sex-sorting facility is a long distance from the semen collection place and to overcome this difficulty two freeze-thaw cycles may be necessary. In this study, effects of refreezing on brown bear electroejaculated spermatozoa were analyzed. We carried out two experiments: (1) to assess the effects of the two freezing-thawing cycles on sperm quality and to analyze three different elapsed times between freezing-thawing cycles (30, 90 and 180 min), and (2) to analyze the use of PureSperm between freezing-thawing cycles to select a more motile and viable sperm subpopulation which better survived first freezing. The motility, viability and undamaged acrosomes were significantly reduced after the second thawing respect to first thawing into each elapsed time group, but the elapsed times did not significantly affect the viability and acrosome status although motility was damaged. Our results with the PureSperm gradient showed higher values of viability in freezability of select sample (pellet) respect to the rest of the groups and it also showed a significant decrease in the number of acrosome damaged. In summary, the double freezing of bear semen selected by gradient centrifugation is qualitatively efficient, and thus could be useful to carry out a sex-sorting of frozen-thawed bear spermatozoa before to send the cryopreserved sample to a biobank. Given the low recovery of spermatozoa after applying a selection gradient, further studies will be needed to increase the recovery rate without damaging of the cell quality.

Freezing of rat tibiae at -20°c does not affect the mechanical properties of intramedullary bone/implant-interface: brief report.

BACKGROUND: The effects of freezing-thawing cycles on intramedullary bone-implant interfaces have been studied in a rat model in mechanical pull-out tests. IMPLANTS: Twenty TiAl6V4 rods (Ø 0.8 mm, length 10 mm) implanted in rat tibiae METHODS: 10 rats underwent bilateral tibial implantation of titanium rods. At eight weeks, the animals were sacrificed and tibiae harvested for biomechanical testing. Eight tibiae were frozen and stored at -20°C for 14 days, the remaining eight were evaluated immediately post-harvest. Pull-out tests were used to determine maximum force and interfacial shear strength. RESULTS: There were no significant differences between fresh and those of the frozen-thawed group in maximum force or in interfacial shear strength. CONCLUSION: Frozen Storage of rat tibiae containing implants at -20° C has no effects on the biomechanical properties of Bone/ Implant interface.