Signatures of selective sweeps in continuous-space populations.

Selective sweeps describe the process by which an adaptive mutation arises and rapidly fixes in the population, thereby removing genetic variation in its genomic vicinity. The expected signatures of selective sweeps are relatively well understood in panmictic population models, yet natural populations often extend across larger geographic ranges where individuals are more likely to mate with those born nearby. To investigate how such spatial population structure can affect sweep dynamics and signatures, we simulated selective sweeps in populations inhabiting a two-dimensional continuous landscape. The maximum dispersal distance of offspring from their parents can be varied in our simulations from an essentially panmictic population to scenarios with increasingly limited dispersal. We find that in low-dispersal populations, adaptive mutations spread more slowly than in panmictic ones, while recombination becomes less effective at breaking up genetic linkage around the sweep locus. Together, these factors result in a trough of reduced genetic diversity around the sweep locus that looks very similar across dispersal rates. We also find that the site frequency spectrum around hard sweeps in low-dispersal populations becomes enriched for intermediate-frequency variants, making these sweeps appear softer than they are. Furthermore, haplotype heterozygosity at the sweep locus tends to be elevated in low-dispersal scenarios as compared to panmixia, contrary to what we observe in neutral scenarios without sweeps. The haplotype patterns generated by these hard sweeps in low-dispersal populations can resemble soft sweeps from standing genetic variation that arose from substantially older alleles. Our results highlight the need for better accounting for spatial population structure when making inferences about selective sweeps.

Rehabilitation exercise quality assessment through supervised contrastive learning with hard and soft negatives.

Exercise-based rehabilitation programs have proven to be effective in enhancing the quality of life and reducing mortality and rehospitalization rates. AI-driven virtual rehabilitation, which allows patients to independently complete exercises at home, utilizes AI algorithms to analyze exercise data, providing feedback to patients and updating clinicians on their progress. These programs commonly prescribe a variety of exercise types, leading to a distinct challenge in rehabilitation exercise assessment datasets: while abundant in overall training samples, these datasets often have a limited number of samples for each individual exercise type. This disparity hampers the ability of existing approaches to train generalizable models with such a small sample size per exercise type. Addressing this issue, this paper introduces a novel supervised contrastive learning framework with hard and soft negative samples that effectively utilizes the entire dataset to train a single model applicable to all exercise types. This model, with a Spatial-Temporal Graph Convolutional Network (ST-GCN) architecture, demonstrated enhanced generalizability across exercises and a decrease in overall complexity. Through extensive experiments on three publicly available rehabilitation exercise assessment datasets, UI-PRMD, IRDS, and KIMORE, our method has proven to surpass existing methods, setting a new benchmark in rehabilitation exercise quality assessment.

Scientific and technological analysis of exchange-spring magnets: Applications and trends.

In this work, a bibliometric study was carried out to perform a scientific and technological analysis of exchange-spring magnets, an alternative permanent magnet synthesized by reducing or eliminating the use of critical raw materials, such as rare earths. The bibliometric analysis utilized the Scopus database, Orbit-Intellixir, VOSviewer, Orbit-Intelligence and Loglet Lab 4 software for maturity analysis, keyword network representations, charts and graphs for scientific articles and/or patents. A special analysis was performed on nanocomposite and thin-films systems based on Nd-Fe-B, SmCo5 and Mn-Al-C alloys, either mixed or layered with a soft magnetic phase, where relevant information on their magnetic parameters was compilated in tables, highlighting the nanostructured systems that have been exhibited the best permanent magnet properties. The bibliometric analysis revealed that the primary production of scientific articles is concentrated in industrialized countries, and they are predominantly published in journals dedicated to magnetism. A patents analysis showed that Nissan motors is by far the main applicant, with most of its patents is focused on technological domains related to electrical machinery, apparatus, energy and metallurgy. On the other hand, the S-curve of maturity for scientific articles indicated that the study of exchange-spring magnets is entering a mature state. In contrast, patent production, following a bi-logistic model, is in a saturation stage for the second S-curve. Maturity analyses, employing S-curve, bi-logistic and multi-logistic models, were performed on nanocomposites and thin films based on Nd-Fe-B, SmCo5 and Mn-Al-C alloys, respectively. We found that the investigation in Nd-Fe-B-based alloys is close to enter to a scientific saturation stage, while an average growth stage is observed for the SmCo5 and Mn-Al-C-based alloys. This suggests that research on alternative magnets, capable of fulfilling technological applications where a Nd-Fe-B magnets are commonly used, is a topic of significant interest.

A Sustainable Multi-Objective Model for Capacitated-Electric-Vehicle-Routing-Problem Considering Hard and Soft Time Windows as Well as Partial Recharging.

Due to the high pollution of the transportation sector, nowadays the role of electric vehicles has been noticed more and more by governments, organizations, and environmentally friendly people. On the other hand, the problem of electric vehicle routing (EVRP) has been widely studied in recent years. This paper deals with an extended version of EVRP, in which electric vehicles (EVs) deliver goods to customers. The limited battery capacity of EVs causes their operational domains to be less than those of gasoline vehicles. For this purpose, several charging stations are considered in this study for EVs. In addition, depending on the operational domain, a full charge may not be needed, which reduces the operation time. Therefore, partial recharging is also taken into account in the present research. This problem is formulated as a multi-objective integer linear programming model, whose objective functions include economic, environmental, and social aspects. Then, the preemptive fuzzy goal programming method (PFGP) is exploited as an exact method to solve small-sized problems. Also, two hybrid meta-heuristic algorithms inspired by nature, including MOSA, MOGWO, MOPSO, and NSGAII_TLBO, are utilized to solve large-sized problems. The results obtained from solving the numerous test problems demonstrate that the hybrid meta-heuristic algorithm can provide efficient solutions in terms of quality and non-dominated solutions in all test problems. In addition, the performance of the algorithms was compared in terms of four indexes: time, MID, MOCV, and HV. Moreover, statistical analysis is performed to investigate whether there is a significant difference between the performance of the algorithms. The results indicate that the MOSA algorithm performs better in terms of the time index. On the other hand, the NSGA-II-TLBO algorithm outperforms in terms of the MID, MOCV, and HV indexes.

Automated reconstruction: Predictive models based on facial morphology matrices.

Forensic Facial Approximation (FFA) has evolved, with techniques advancing to refine the intercorrelation between the soft-tissue facial profile and the underlying skull. FFA has become essential for identifying unknown persons in South Africa, where the high number of migrant and illegal labourers and many unidentified remains make the identification process challenging. However, existing FFA methods are based on American or European standards, rendering them inapplicable in a South African context. We addressed this issue by conducting a study to create prediction models based on the relationships between facial morphology and known factors, such as population affinity, sex, and age, in white South African and French samples. We retrospectively collected 184 adult cone beam computed tomography (CBCT) scans representing 76 white South Africans (29 males and 47 females) and 108 French nationals (54 males and 54 females) to develop predictive statistical models using a projection onto latent structures regression algorithm (PLSR). On training and untrained datasets, the accuracy of the estimated soft-tissue shape of the ears, eyes, nose, and mouth was measured using metric deviations. The predictive models were optimized by integrating additional variables such as sex and age. Based on trained data, the prediction errors for the ears, eyes, nose, and mouth ranged between 1.6 mm and 4.1 mm for white South Africans; for the French group, they ranged between 1.9 mm and 4.2 mm. Prediction errors on non-trained data ranged between 1.6 mm and 4.3 mm for white South Africans, whereas prediction errors ranging between 1.8 mm and 4.3 mm were observed for the French. Ultimately, our study provided promising predictive models. Although the statistical models can be improved, the inherent variability among individuals restricts the accuracy of FFA. The predictive validity of the models was improved by including sex and age variables and considering population affinity. By integrating these factors, more customized and accurate predictive models can be developed, ultimately strengthening the effectiveness of forensic analysis in the South African region.

Angina Bullosa Hemorrhagica of the Oral Cavity: A Case Report.

Angina bullosa hemorrhagica (ABH) is a rare, benign condition that is characterized by a blood-filled blister in the oropharynx. This report describes the case of a 27-year-old male who developed a blister in the oral cavity that ruptured abruptly and resolved spontaneously. The diagnosis of ABH was made according to the diagnostic criteria of Ordioni et al. The core motive of this report is to demonstrate the clinical features and course of ABH to avoid misdiagnosis.

Protein corona, influence on drug delivery system and its improvement strategy: A review.

Nano drug delivery systems offer several benefits, including enhancing drug solubility, regulating drug release, prolonging drug circulation time, and minimized toxicity and side effects. However, upon entering the bloodstream, nanoparticles (NPs) encounter a complex biological environment and get absorbed by various biological components, primarily proteins, leading to the formation of a 'Protein Corona'. The formation of the protein corona is affected by the characteristics of NPs, the physiological environment, and experimental design, which in turn affects of the immunotoxicity, specific recognition, cell uptake, and drug release of NPs. To improve the abundance of a specific protein on NPs, researchers have explored pre-coating, modifying, or wrapping NPs with the cell membrane to reduce protein adsorption. This paper, we have reviewed studies of the protein corona in recent years, summarized the formation and detection methods of the protein corona, the effect of the protein corona composition on the fate of NPs, and the design of new drug delivery systems based on the optimization of protein corona to provide a reference for further study of the protein corona and a theoretical basis for the clinical transformation of NPs.

Maxillary labial peri-implant hard and soft tissue alteration observed on cross-sectional dimension: a 2-year prospective observational study.

OBJECTIVES: To evaluate how peri-implant hard and soft tissue height (BH, MH) alter after final prostheses placement related to labial hard and soft tissue thickness (BW, MW). MATERIALS AND METHODS: Forty-five platform-switched implants were classified into four groups according to BW and MW: type 1 (thick BW and thick MW), type 2 (thick BW and thin MW), type 3 (thin BW and thick MW), type 4 (thin BW and thin MW). Tissue resorption was evaluated on cone-beam CT images taken at final prostheses placement, at 1-year follow-up, and at 2-year follow-up. Kruskal-Wallis test and post hoc Mann-Whitney test were applied; significance was set to 0.05. RESULTS: BH resorption was 0.13 ± 0.12 mm in type 1, 0.26 ± 0.17 mm in type 2, 0.09 ± 0.09 mm in type 3, 0.94 ± 0.19 mm in type 4. Differences between type 1 and 4, type 2 and 4, and type 3 and 4 were statistically significant (p < 0.001, p = 0.005, p < 0.001, respectively). MH resorption was 0.10 ± 0.09 mm in type 1, 0.36 ± 0.16 mm in type 2, 0.12 ± 0.12 mm in Type 3, 0.79 ± 0.23 mm in type 4. Differences between type 1 and 2, type 1 and 4, type 2 and 3, type 2 and 4 and type 3 and 4 were statistically significant (p < 0.001). CONCLUSIONS: Significantly less BH/MH resorption occurs around implants with thick BW/MW than those with thin BW/MW in 2 years. Implants with thick peri-implant soft tissue resulted in significantly less tissue resorption in second year after final prostheses placement.

Reactivity of a Cationic Bismuth Amide towards Unsymmetric Heterocumulenes.

The reactivity of a literature-known, ring-strained bismuth amide cation towards a range of unsymmetric heterocumulene substrates has been investigated. Reactions with ketenes R2 C=C=O (R=Me, Ph), isocyanates R'N=C=O, and isothiocyanates R'N=C=S (R'=Ph, 4-CF3 -C6 H4 ) proceed via facile insertion of the heterocumulene in the Bi-N bond of the cationic bismuth amide. Unexpectedly pronounced differences in the regioselectivity of these insertion reactions have been observed, yielding a rich variety of heterocycle motifs (BiC2 NC2 , BiC2 NCO, BiC2 NCS, BiC2 NCN), some of which are unprecedented. Parameters that control the regioselectivity of the insertion reactions have been identified and are discussed based on experimental and theoretical investigations. Analytical techniques applied in this work include heteronuclear and two-dimensional NMR spectroscopy, IR spectroscopy, elemental analysis, single-crystal X-ray diffraction analyses, and DFT calculations.

Pearson's principle-inspired hollow metal sulfide for amplified photoelectrochemical immunoassay for disease-related protein.

Designing a universal route for rational synthesis of a family of hollow multinary chalcogenide semiconductors for photoelectrochemical biosensors is still facing to the enormous challenges ahead. Herein a template-assisted Cu2O surface vulcanization and etching through a Pearson's hard and soft acid-base (HSAB) principle was utilized to synthesize hollow Cu2-xS photoactive materials for photocurrent detection of prostate-specific antigen (PSA). We initially synthesized cubic Cu2O and further surface sulfidation and HCl etching to obtain cubic Cu2-xS. Inspiringly, stirring of CuS, phosphine (TBP: tributylphosphine) and other metal salts could replace Cu+ ions to obtain new metal sulfides without changing the framework, size and thickness of the original material. This interesting phenomenon could be explained by HSAB theory, which soft base was favorable for combining soft acid (Cu+) to drive Cu+ out of the framework. Based on the results, HSAB-based reaction system was applied to develop novel photoelectrochemical PSA immunoassay. Polymetallic-doped sulfides (ZnxCd1-xS) had better photocurrent response than pure binary sulfides. A copper oxide (CuO)-labeled detection antibody is captured in a microplate along with a sandwich immunoassay in the presence of target PSA. Subsequently, the CuO nanoparticles were dissociated by hydrochloric acid, releasing a large amount of copper ions to participate in the cation exchange reaction with ZnxCd1-xS. Such excellent photoelectric conversion materials could sensitively detect target PSA with a wide linear range from 1.0 pg/mL to 10 ng/mL at a limit of detection down to 0.32 pg/mL. Additionally, favorable stability, great anti-interference ability, easy-fabrication, low-cost, and satisfactory accuracy for the analysis of actual samples were acquired. Importantly, the concept of cation exchange reaction can be widely used to synthesize advanced nanomaterials for fabrication of high-efficiency biosensing systems.

Subjective emotion trajectories in couple therapy and associations with improvement in relationship satisfaction.

Existing couple therapies are generally effective for reducing romantic relationship distress and divorce, but therapy outcomes remain poor for many. Outcomes can be improved through greater understanding of session-by-session therapeutic processes, particularly in real-world treatment settings. Modern couple therapy models commonly emphasize the importance of emotional experiences as key change processes, yet few empirical studies have tested the merits of this focus. The present study addresses this limitation by examining trajectories of subjective emotions and their association with change in a key relationship outcome, relationship satisfaction, among military veterans and their partners at a VA Medical Center. Partners rated their relationship satisfaction prior to couple therapy sessions and subjective emotions immediately after sessions. Consistent with hypotheses, both hard (e.g., anger) and soft (e.g., sadness) negative emotions decreased significantly over the course of therapy. Those couples with greater decreases in hard negative, but not soft negative, emotions showed significantly more improvement in relationship satisfaction. Positive emotions did not significantly change across couples in general, but those couples whose positive emotions did increase also showed more improvement in relationship satisfaction. These results suggest change in subjective emotions may be one process underlying improvement in couple therapy and lend empirical support to the emphasis on emotion-based change processes underlying acceptance-based and emotion-focused couple therapies.

Effects of local versus global competition on reproductive skew and sex differences in social dominance behaviour.

Social hierarchies are often found in group-living animals. The hierarchy position can influence reproductive success (RS), with a skew towards high-ranking individuals. The amount of aggression in social dominance varies greatly, both between species and between males and females within species. Using game theory we study this variation by taking into account the degree to which reproductive competition in a social group is mainly local to the group, emphasizing within-group relative RS, or global to a larger population, emphasizing an individual's absolute RS. Our model is similar to recent approaches in that reinforcement learning is used as a behavioural mechanism allowing social-hierarchy formation. We test two hypotheses. The first is that local competition should favour the evolution of mating or foraging interference, and thus of reproductive skew. Second, decreases in reproductive output caused by an individual's accumulated fighting damage, such as reduced parenting ability, will favour less intense aggression but should have little influence on reproductive skew. From individual-based simulations of the evolution of social dominance and interference, we find support for both hypotheses. We discuss to what extent our results can explain observed sex differences in reproductive skew and social dominance behaviour.

Applying the task-technology fit model to construct the prototype of a medical staff scheduling system.

BACKGROUND: Medical staff scheduling problems are complex and involve numerous constraints. OBJECTIVE: This research uses the task-technology fit (TTF) model to measure the technology characteristics of information technology (IT) systems as a reference for constructing a prototype for a medical staff scheduling system to identify function requirements and design human interfaces. METHOD: After the evaluation of the proposed scheduling system, this research excludes compatibility from the 13 technology characteristics and adds two technology characteristics for consideration: customization and scalability. RESULTS: Based on the revised technology characteristics of the TTF model, this research develops flexible scheduling functions to satisfy daily manpower requirements and allow predetermined schedules and day-off reservations for a hospital's radiological technologists. Characterized by flexibility, customization, and scalability, the system can accommodate several algorithms to generate a better schedule that satisfies hard and soft constraints. Furthermore, the scheduler can choose the required hard and soft constraints from all constraints. The prototype of the scheduling system will be easily extended to add or modify constraints in the case of requirement or regulation changes. CONCLUSION: The results of this study provide a prototype for system developers to design a customized staff scheduling system for each medical unit.

Interface Modification with CuCrO2 Nanocrystals for Highly Efficient and Stable Planar Perovskite Solar Cells.

The interfaces between the absorber and charge transport layers are shown to be critical for the performance of perovskite solar cells (PSCs). PSCs based on the Spiro-OMeTAD hole transport layers generally suffer from the problems of stability and reproducibility. Inorganic hole transport materials CuCrO2 have good chemical stability and high hole mobility. Herein, we reported the preparation of the delafossite-type CuCrO2 nanocrystals with a template-etching-calcination method and the incorporation of the as-obtained CuCrO2 nanocrystals at the perovskite/Spiro-OMeTAD interfaces of planar PSCs to improve the device efficiency and stability. Compared with the traditional hydrothermal method, the template-etching-calcination method used less calcination time to prepare CuCrO2 nanocrystals. After the CuCrO2 interface modification, the efficiency of PSCs improved from 18.08% to 20.66%. Additionally, the CuCrO2-modified PSCs showed good stability by retaining nearly 90% of the initial PCE after being stored in a drybox for 30 days. The template-etching-calcination strategy will pave a new approach for the synthesis of high-performance inorganic hole-transporting materials.

In vivo neurophysiological assessment of in silico predictions of neurotoxicity: Citronellal, 3,4-dichloro-1-butene, and benzyl bromoacetate.

Neurotoxicants may be widespread in the environment and can produce serious health impacts in the human population. Screening programs that use in vitro methods have generated data for thousands of chemicals. However, these methods often do not evaluate repeated or prolonged exposures, which are required for many neurotoxic outcomes. Additionally, the data produced by such screening methods may not include mechanisms which play critical biological roles necessary for in vivo neurotoxicity. The Hard and Soft Acids and Bases (HSAB) in silico model focuses on chemical structure and electrophilic properties which are important to the formation of protein adducts. A group of structurally diverse chemicals have been evaluated with an in silico screening approach incorporating HSAB parameters. However, the predictions from the expanded chemical space have not been evaluated using in vivo methods. Three chemicals predicted to be cumulative toxicants were selected for in vivo neurotoxicological testing. Adult male Long-Evans rats were treated orally with citronellal (CIT), 3,4-dichloro-1-butene (DCB), or benzyl bromoacetate (BBA) for 8 weeks. Behavioral observations were recorded weekly to assess motor function. Peripheral neurophysiological measurements were derived from nerve excitability (NE) tests which involved compound muscle action potentials (CMAPs) in the tail and foot, and mixed nerve action potentials (MNAPs) in the tail. Compound nerve action potentials (CNAPs) and nerve conduction velocity (NCV) in the tail were also quantified. Peripheral inputs into the central nervous system were examined using somatosensory evoked potentials recorded from the cortex (SEPCTX) and cerebellum (SEPCEREB). CIT or BBA did not result in significant alterations to peripheral nerve or somatosensory function. DCB reduced grip-strength and altered peripheral nerve function. The MNAPs required less current to reach 50% amplitude and had a lower calculated rheobase, suggesting increased excitability. Increased CNAP amplitudes and greater NCV were also observed. Novel changes were found in the SEPCTX with an abnormal peak forming in the early portion of the waveforms of treated rats, and decreased latencies and increased amplitudes were observed in SEPCEREB recordings. These data contribute to testing an expanded chemical space from an in silico HSAB model for predicting cumulative neurotoxicity and may assist with prioritizing chemicals to protect human health.

Preparation, Structure and Properties of Urethane-Containing Elastomers Based on Epoxy Terminal Oligomers.

The effect of polyester oligoethylene adipate molecular weight, diisocyanate structure, and chain extender on the properties of epoxyurethane-based oligomer elastomers was studied in this research. Oligoethylene adipates were obtained via polycondensation of adipic acid and ethylene glycol. Epoxyurethane oligomers were synthesized according to a two-step route with an oligodiisocyanate as an intermediate product. The elastomers with hard urethane hydroxyl blocks were synthesized from oligodiisocyanates. The deformation and strength properties of the elastomers were studied.

Long-term hard and soft tissue response following isolated genioplasty: a systematic review.

PURPOSE: Genioplasty facilitates alteration of the chin position and contour which contributes to aesthetics and function. The response of the hard and soft tissues following genioplasty has not been assessed after a year or more of the surgery being performed. Hence, the aim of this systematic review was to assess the response of the hard and soft tissues occurring at least 1 year after the procedure. MATERIAL AND METHODS: A literature search was conducted in the following electronic databases: PubMed, Ovid, LILACS, and Cochrane Library. Potential articles were identified wherein only studies with genioplasty performed as an isolated procedure and with data at least 12 months after the procedure were included. RESULTS: Five studies were included in this systematic review. Two of the articles included were considered to be of good quality while three were considered to be of moderate quality using a modified Downs and Black tool. The ROBINS-I tool showed a moderate risk of bias for most domains. The study characteristics revealed varying degrees of relapse for the hard and soft tissues. CONCLUSIONS: In the anteroposterior plane, the soft tissue relapsed more than the hard tissues 3 years post genioplasty. However, relapse in the vertical plane showed a wide variation for both the hard and soft tissues. In the anteroposterior plane, the hard tissue to soft tissue response 2 years or more following genioplasty ranged from 1:0.77 to 1:0.91 while in the vertical plane the hard tissue to soft tissue response ranged from 1:0.67 to 1:1.16.

How to reveal disguised paternalism: version 2.0.

BACKGROUND: We aim to further develop an index for detecting disguised paternalism, which might influence physicians' evaluations of whether or not a patient is decision-competent at the end of life. Disguised paternalism can be actualized when physicians transform hard paternalism into soft paternalism by questioning the patient's decision-making competence. METHODS: A previously presented index, based on a cross-sectional study, was further developed to make it possible to distinguish between high and low degrees of disguised paternalism using the average index of the whole sample. We recalculated the results from a 2007 study for comparison to a new study conducted in 2020. Both studies are about physicians' attitudes towards, and arguments for or against, physician-assisted suicide. RESULTS: The 2020 study showed that geriatricians, palliativists, and middle-aged physicians (46-60 years old) had indices indicating disguised paternalism, in contrast with the results from the 2007 study, which showed that all specialties (apart from GPs and surgeons) had indices indicating high degrees of disguised paternalism. CONCLUSIONS: The proposed index for identifying disguised paternalism reflects the attitude of a group towards physician assisted suicide. The indices make it possible to compare the various medical specialties and age groups from the 2007 study with the 2020 study. Because disguised paternalism might have clinical consequences for the rights of competent patients to participate in decision-making, it is important to reveal disguised hard paternalism, which could masquerade as soft paternalism and thereby manifest in practice. Methods for improving measures of disguised paternalism are worthy of further development.

Chromosome X-wide Analysis of Positive Selection in Human Populations: Common and Private Signals of Selection and its Impact on Inactivated Genes and Enhancers.

The ability of detecting adaptive (positive) selection in the genome has opened the possibility of understanding the genetic basis of population-specific adaptations genome-wide. Here, we present the analysis of recent selective sweeps, specifically in the X chromosome, in human populations from the third phase of the 1,000 Genomes Project using three different haplotype-based statistics. We describe instances of recent positive selection that fit the criteria of hard or soft sweeps, and detect a higher number of events among sub-Saharan Africans than non-Africans (Europe and East Asia). A global enrichment of neural-related processes is observed and numerous genes related to fertility appear among the top candidates, reflecting the importance of reproduction in human evolution. Commonalities with previously reported genes under positive selection are found, while particularly strong new signals are reported in specific populations or shared across different continental groups. We report an enrichment of signals in genes that escape X chromosome inactivation, which may contribute to the differentiation between sexes. We also provide evidence of a widespread presence of soft-sweep-like signatures across the chromosome and a global enrichment of highly scoring regions that overlap potential regulatory elements. Among these, enhancers-like signatures seem to present putative signals of positive selection which might be in concordance with selection in their target genes. Also, particularly strong signals appear in regulatory regions that show differential activities, which might point to population-specific regulatory adaptations.

Synthesis and Catalytic Activity of Atrane-type Hard and Soft Lewis Superacids with a Silyl, Germyl, or Stannyl Cationic Center.

The synthesis and isolation of atrane-type molecules 1E+ (E=Si, Ge, or Sn) having a cationic group 14 elemental center are reported. The cations 1E+ act as hard and soft Lewis superacids, which readily interact with various hard and soft Lewis basic substrates. The rigid atrane framework stabilizes the localized positive charge on the elemental center and assists the formation of the well-defined highly coordinated states of 1E+ . The cations were applied to the hydrodefluorination, Friedel-Crafts reaction, alkyne cyclization, and carbonyl reduction as Lewis acid catalysts. Most notably, [1Si][ClO4 ] exhibits unique chemoselectivity that depends on a solvent in the competitive reaction of silyl enol ether with a mixture of benzaldehyde dimethyl acetal and benzaldehyde. Our findings indicate the potential of hard and soft Lewis superacids in organic synthesis.