Sediment resuspension as a driving force for organic carbon transference and rebalance in marginal seas.

The transfer of particulate organic carbon (POC) to dissolved organic carbon (DOC; OC transferP-D) is crucial for the marine carbon cycle. Sediment resuspension driven by hydrodynamic forcing can affect the burial of sedimentary POC and benthic biological processes in marginal sea. However, the role of sediment grain size fraction on OC transferP-D and the subsequent impact on OC cycling remain unknown. Here, we conduct sediment resuspension simulations by resuspending grain-size fractionated sediments (< 20, 20-63, and > 63 µm) into filtered seawater, combined with analyses of OC content, optical characteristics, 13C and 14C isotope compositions, and molecular dynamics simulations to investigate OC transferP-D and its regulations on OC bioavailability under sediment resuspension. Our results show that the relative intensities of terrestrial humic-like OC (refractory DOC) increase in resuspension experiments of < 20, 20-63, and > 63 µm sediments by 0.14, 0.01, and 0.03, respectively, likely suggesting that sediment resuspension drives refractory DOC transfer into seawater. The variations in the relative intensities of microbial protein-like DOC are linked to the change of terrestrial humic-like OC, accompanied by higher DOC content and reactivity in seawater, particularly in finer sediments resuspension experiments. This implies that transferred DOC likely fuels microbial growth, contributing to the subsequent enhancement of DOC bioavailability in seawater. Our results also show that the POC contents increase by 0.35 %, 0.66 %, and 0.93 % in < 20, 20-63, and > 63 µm resuspension experiments at the end of incubation, respectively. This suggests that the re-absorption of OC on particles may be a significant process, but previously unrecognized during sediment resuspension. Overall, our findings suggest that sediment resuspension promotes the OC transferP-D, and the magnitudes of OC transferP-D further influence the DOC and POC properties by inducing microbial production and respiration. These processes significantly affect the dynamics and recycling of biological carbon pump in shallow marginal seas.

Enhancement of carbon sink in the main marginal sea ice zone by cold season Arctic cyclones.

The Arctic Ocean, as a significant carbon sink, is attracting increased attention within the scientific community. This study focused on the main marginal sea ice zone, which has been the most sensitive to environmental changes in recent decades. Using data from reanalysis, models, and on-site observations, the changes in air-sea CO2 flux (FCO2) were analyzed during the influence of Arctic cyclones (ACs) in 2021-2022. Results indicated that the passage of ACs tended to increase the average carbon sink in the main marginal ice zone, with a more pronounced effect during the cold season. During ACs, the average FCO2 could reach -6.95 mmolC m-2 d-1. This was mainly associated with the stronger and more concentrated distribution of ACs where there was lower pCO2 (air-sea gradient of CO2 partial pressure) in the cold season. Additionally, the change in FCO2 during ACs was primarily affected by the sea surface wind and sea-ice concentration in the cold season, while it was influenced by a variety of environmental factors in the warm season, including the sea surface wind, sea-ice concentration, and ecological factors.

Distribution and sources of polycyclic aromatic hydrocarbons in surface sediments of the East China marginal seas: Significance of the terrestrial input and shelf mud deposition.

To investigate the distribution, sources, influencing factors, and ecological risk of polycyclic aromatic hydrocarbons (PAHs) in East China Marginal Seas (ECMSs) sediments, we measured the concentrations of 16 PAHs in 104 surface sediment samples collected from the ECMSs in 2014 and 2016. Total PAH concentration (∑PAHs) ranged from 4.49 to 163.66 ng/g dry weight (dry w), with 65.98 ± 33.00 (mean ± SD) ng/g dry w. The highest PAH concentrations and total organic carbon were observed in areas with fine-grained sediments in the Bohai Sea (BS), Yellow Sea (YS), and coastal East China Sea (ECS), indicating the prominent influence of regional hydrodynamics and sediment properties. The dominant PAH congener in BS and YS was BbF, whereas coastal ECS was Phe. The heterogeneity of PAH sources implies that terrestrial PAH input and shelf mud deposition have crucial roles in the source-sink processes of PAHs in a strongly human-influenced marginal sea.

Acidity in rainwater and airborne suspended particles in the southwestern coast of the East Sea (Sea of Japan): Their potential impact on seawater total alkalinity.

Our understanding of the impact of atmospheric acid deposition on marine carbonate system remains limited, largely due to a lack of data regarding acidity present in atmospheric particles and precipitation. Previous research has relied on the electroneutrality-based ion balance method for indirect estimation of atmospheric acidity. In this study, atmospheric samples collected at a coastal site of South Korea were mixed with seawater to measure the change in seawater total alkalinity (ΔTAAPL) associated with atmospheric proton loading. For the precipitation samples, the measured ΔTAAPL and electroneutrality-based estimates showed a significant correlation. However, we did not observe similar results for the atmospheric particle samples. Furthermore, the decrease in oceanic TA due to ΔTAAPL was substantially smaller than that in dissolved inorganic carbon from concurrent nitrogen fertilization. Consequently, the adverse impact of acid deposition on ocean acidification or air-sea exchange of CO2 appears to be insignificant on a short-term scale.

Biogeochemical and physical controls on ammonium accumulation on the Chukchi shelf, western Arctic Ocean.

Spatial variability of ammonium concentrations along repeat transects were examined on the Chukchi shelf during 2012-2018. Two distinct near-bottom high ammonium pools (>1 µmol/kg) near 67.5°N and 72.5°N of the transects were identified in all years. The accumulation of ammonium in the regions is driven primarily by a combination of biogeochemical processes (e.g., dynamic bacterial remineralization of organic matter) and physical controls (e.g., strong density-contrast barrier limits upward mixing of ammonium). The ammonium pool on the shelf may became larger in the expectation of the stronger bacterial remineralization following elevate primary production, and may have potential impact on the structure and productivity of ecosystem on the Chukchi shelf.

Regional background determination and pollution assessment of heavy metals in the semi-closed Bohai Sea sediments.

Background metal concentrations are important in assessing pollution level of marine sediments; however, they can be significantly altered by local depositional environments, resulting in significant errors in regional pollution assessment. This study was based on the investigation of the background levels of heavy metals in the Bohai Sea sediments using sediment core, 2-sigma outlier, and regression methods. We also estimate the ecological risks of heavy metals for surface sediments collected from the Bohai Sea using the three methods mentioned above. Ecological risks of heavy metals calculated using the regression method show wide disparities and significant differences from those calculated using the sediment core and 2-sigma methods, indicating that the regression method is not suitable for the Bohai Sea, likely as a result of its complex sources. Conversely, the estimated ecological risks using the sediment core method are moderate, and most heavy metals, except for Hg and Cd, have negligible contamination.

Lignin-oxidizing and xylan-hydrolyzing Vibrio involved in the mineralization of plant detritus in the continental slope.

A large amount of terrigenous organic matter (TOM) is constantly transported to the deep sea. However, relatively little is known about the microbial mineralization of TOM therein. Our recent in situ enrichment experiments revealed that Vibrio is especially enriched as one of the predominant taxa in the cultures amended with natural plant materials in the deep sea. Yet their role in the mineralization of plant-derived TOM in the deep sea remains largely unknown. Here we isolated Vibrio strains representing dominant members of the enrichments and verified their potential to degrade lignin and xylan. The isolated strains were closely related to Vibrio harveyi, V. alginolyticus, V. diabolicus, and V. parahaemolyticus. Extracellular enzyme assays, and genome and transcriptome analyses revealed diverse peroxidases, including lignin peroxidase (LiP), catalase-peroxidase (KatG), and decolorizing peroxidase (DyP), which played an important role in the depolymerization and oxidation of lignin. Superoxide dismutase was found to likely promote lignin oxidation by supplying H2O2 to LiP, DyP, and KatG. Interestingly, these deep-sea Vibrio strains could oxidize lignin and hydrolyze xylan not only through aerobic pathway, but also through anaerobic pathway. Genome analysis revealed multiple anaerobic respiratory mechanisms, including the reductions of nitrate, arsenate, tetrathionate, and dimethyl sulfoxide. The strains showed the potential to anaerobically reduce sulfite and metal oxides of iron and manganese, in contrast the non-deep-sea Vibrio strains were not retrieved of genes involved in reduction of metal oxides. This is the first report about the lignin oxidation mechanisms in Vibrio and their role in TOM mineralization in anoxic and oxic environments of the marginal sea.

Exploring origin of oxygen-consuming organic matter in a newly developed quasi-hypoxic coastal ocean, the Bohai Sea (China): A stable carbon isotope perspective.

The Bohai Sea, adjacent to the Northwest Pacific, is a semi-enclosed shallow-water marginal sea that was considered on a critical path of eutrophication and environmental degradation. To better understand the Bohai Sea metabolism-induced summertime dissolved oxygen (DO) decline, five field surveys were conducted between July 2019 and July 2021 to investigate the seasonal/interannual and spatial variations in DO, dissolved inorganic carbon (DIC), and stable isotopic composition of DIC (δ13CDIC). Although the water-mixing scheme was subject to spatial variation, a uniform apparent ratio of δ13CDIC versus apparent oxygen utilization was estimated at -0.0122‰ per µmol O2 kg-1 in the Bohai Sea in summer. Based on a three-endmember water-mixing model and the mass balance of DIC and its stable isotopic composition, the assumed uniform δ13C values of oxygen-consuming organic matter in the Bohai Sea DO-deficient areas was estimated to be -19.47 ± 1.85‰ in 2020 and between -20.6‰ and - 18.1‰ in 2021. This isotopic composition is very similar to the δ13C value of organic matter from marine diatoms, but different to that of terrestrial organic matter sources surrounding the Bohai Sea. Our results indicate that nearly all the organic matter consumed by community respiration in the Bohai Sea is produced in situ by marine plankton. To mitigate the seasonal DO shortage in the Bohai Sea, reduction of allochthonous nutrients is crucial.

Phylogenetic Structure of Synechococcus Assemblages and Its Environmental Determinants in the Bay and Strait Areas of a Continental Sea.

Marine Synechococcus, a significant contributor to primary production, shows high phylogenetic diversity. However, studies on its phylogenetic composition in the Bohai Sea, the largest continental sea in China, are lacking. We sequenced rpoC1 (encodes the RNA polymerase ß' subunit protein) in samples from the Laizhou Bay (LZB) and Bohai Strait (BS) in June and November using high-throughput sequencing to reveal the phylogenetic composition of Synechococcus assemblages in the bay and strait areas of the Bohai Sea. In total, 12 lineages representing Synechococcus subclusters S5.1, S5.2, and S5.3 were identified. Spatially, clade I was obligately dominant in BS. In contrast, the Synechococcus assemblage in LZB was more diverse, with clades VI and III being highly abundant. In addition, we detected strong variation in Synechococcus structure between June and November in the Bohai Sea. Clades II, III, XX, and miyav were only detected in November. Vertically, variation in Synechococcus assemblage was not apparent among the water layers probably due to the shallow water depth with intense water mixing. Results of redundancy analysis (RDA) and random forest (RF) analysis together highlighted the key role of silicate in the Synechococcus assemblage. Our results suggested that the Bohai Sea provides various niches for different Synechococcus clades, resulting in a special phylogenetic composition of the Synechococcus assemblage, compared with that in the adjacent shelf sea and other continental seas in the world.

Nutrient exchanges at the sediment-water interface and the responses to environmental changes in the Yellow Sea and East China Sea.

Release from the sediment is an important nutrient source to the water column of global oceans, especially for marginal seas with active biogeochemical processes. Benthic nutrient biogeochemistry and its responses to environmental changes were investigated in the eastern marginal seas of China using a two-layer diffusion-advection-reaction diagenetic model. Overall, the sediment represented the primary nutrient source with fluxes of ~-342 ± 197, -1.25 ± 0.50, and -114 ± 56 × 108 mol/month for dissolved inorganic nitrogen (DIN), phosphate, and silicate, respectively. This could contribute up to ~42% of nutrients requested by primary production (PP), with a DIN/SiO32-/PO43- molar ratio of 273:91:1, which was higher than that in the overlying water (49:47:1). Future benthic nutrient fluxes were predicted under two environmental change scenarios (increasing and decreasing PP and biogenic silica). Our study may help rebuild nutrient budgets in the future and formulate environmental management policies in marginal seas.

Spatial and temporal trends of anthropogenic carbon storage in typical marginal seas along the Asia continent in the northern hemisphere.

Global climate change is an indisputable fact, and anthropogenic disturbances are the likely driving mechanisms; moreover, marginal seas tend to respond faster than the global ocean. In this study, the transit time distribution method was used to estimate the anthropogenic carbon (Cant) in the typical marginal seas along the west side of North Pacific Ocean and the Arctic Ocean. From the South China Sea (SCS) to the Arctic Ocean (AO), the range of Cant storage gradually increased with latitude. The maximum and minimum rates of ~0.6 mol C·m-2·yr-1, and ~0.2 mol C·m-2·yr-1 were seen in the AO and SCS, respectively. In the short term, warming and decline of ice cover may promote the transfer of excess CO2 from the atmosphere to the water interior; but on a longer time scale, a positive feedback (i.e., reduced CO2 absorption) may occur due to warming. Accordingly, the AO will likely no longer be a CO2 sink in the future when the sea ice disappears completely.

Fate of land-based antibiotic resistance genes in marginal-sea sediment: Territorial differentiation and corresponding drivers.

No large-scale investigations on fate of land-based antibiotic resistance genes (ARGs) in marginal sea have been reported. The Yellow Sea which is an important marginal sea was selected to investigate the fate, territorial differentiation, and drivers of target ARGs in marginal-sea sediments. ARGs might spread from land to sea with the average absolute abundance of total ARGs in marine/coastal sediments reaching 1.23 × 104/9.79 × 104 copies/g. The Yellow Sea Cold Water Mass was firstly observed to possibly have potential inhibition effect on occurrence of ARGs in marine sediments. Marine sediments showed significant difference from coastal sediments by considering ARGs, microbial community, and sediment features. Network analysis showed that interaction between ARGs and microbial community in coastal sediments was more frequent than that in marine sediment. The anthropogenic factors posed high positive effect on ARGs in marine/coastal sediments with contribution coefficient of 0.524/1.094 while bacterial community mainly posed positive effect on ARGs in marine sediments with contribution coefficient of 0.475, illustrating that spread and proliferation of land-based ARGs in marine sediments might be mainly affected by anthropogenic and microbial factors. These findings provided new information on fate and drivers of ARGs in marginal sea.

Controlling factors of annual cycle of dimethylsulfide in the Yellow and East China seas.

We developed a dimethylsulfide (DMS) module coupled to an ecological dynamics model studying the annual DMS cycle of the Yellow and East China seas (YECS). The model results showed that surface DMS concentrations ([DMS]) peaked in August along the coast, and there exhibited several DMS peaks offshore annually. In addition, surface [DMS] were higher in the Yellow Sea than that in the East China Sea. The annual mean surface [DMS] of the YECS reached to 4.55 nmol/L, and oceanic DMS emissions from this sea area was 6.78 µmol/(m2 day). Several sensitivity experiments demonstrated that phytoplankton community and sea water temperature exerted crucial effects on seasonal variations of surface [DMS]; and phytoplankton community or temperature changed the timing of surface DMS peak while photolysis affected the magnitude of [DMS]. Moreover, the effect size of phytoplankton community or water temperature varied spatially.

Occurrence and spatial distribution of organophosphorus flame retardants and plasticizers in the Bohai, Yellow and East China seas.

Organophosphate esters (OPEs) are man-made organic pollutants that are used in flame retardants (FRs), plasticizers, antifoaming and hydraulic agents, and extractants. The demand for FRs in China has increased, thereby rapidly increasing the use of OPEs and hence resulted in its high levels in the environment. In this study, we measured the concentrations of seven OPE congeners in the seawaters of the Bohai Sea (BS), the Yellow Sea (YS), and the East China Sea (ECS). The horizontal and vertical spatial distributions were then analyzed to assess the OPE pollution. The total concentrations of the seven OPE congeners (ΣOPEs) in the three seas ranged from 7.31 to 100 ng L-1. The main OPE compounds were tris-(1-chloro-2-propyl) phosphate (TCPP, 3.97-35.6 ng L-1), tris-(2-chloroethyl) phosphate (TCEP, 0.59-19.8 ng L-1), and triphenylphosphine oxide (TPPO, from below the detection limit to 43.5 ng L-1). The inventory of the ΣOPEs in the BS, YS, and ECS was estimated as 54.2, 513, and 3950 tons, respectively. Horizontally, the ΣOPE concentration was ranked as BS > YS > ECS. In the vertical direction, the OPE concentrations in the surface water were higher than those in the bottom water. Ocean currents and riverine inputs might be the main factors influencing the distributions of the OPEs in these seas. Moreover, a seasonal variation (summer 2015 versus winter 2016) in the OPEs was observed in the YS, which was probably due to anthropogenic influences and hydrological, meteorological, and biological factors.

Pollution status of PAHs in surface sediments from different marginal seas along China Mainland: A quantitative evaluation on a national scale.

China is one of the largest coastal countries in the world, which have all kinds of marginal systems. Studies have reported the sedimentary Polycyclic aromatic hydrocarbons (PAHs) pollution status, including their concentrations, sources and risks, in localized marginal systems, which showed significant differences. Thus, a comprehensive understanding of their pollution in marginal systems along China Mainland is urgently needed on a national scale. In the present study, the concentrations of 16 priority PAHs in surface sediments from 62 different marginal systems along China Mainland were reviewed. Their sources were identified and apportioned, and the health risks and ecological risks were also evaluated. As a result, the total sedimentary PAHs varied in a wide range of 4-3700 ng/g, with the lowest values observed in Kenting National Park in East China Sea and the highest values observed in Daliao River estuary in Bohai Sea. Their concentrations suggested that they were not contaminated-weakly contaminated in most study areas, but were contaminated-heavily contaminated in some pollution hot-spots. Source identification and apportion suggested that the sedimentary PAHs were mainly originated from coal combustion, vehicular emission, natural gas combustion and petrogenic source, but the coal combustion and vehicular emission contributed most to their emission (>90%). Risk assessment suggested that the carcinogenic risks were lower than the upper limit of the acceptable range (10-4), which were acceptable at a large spatial scale. However, for sediments from Qinhuangdao coastal wetland, Daliao River estuary and Yangpu Bay, their carcinogenic risks were higher than 10-4, which will pose high carcinogenic risks for adults. The non-carcinogenic risks were acceptable in all marginal systems with values lower than the safety guideline (<1). In the ecological risk assessment, their concentrations in some pollution hot-spots were higher than the safety guidelines (effects range low, ERL), suggesting a higher potential ecological risk.

Southeastern Yellow Sea as a sink for atmospheric carbon dioxide.

The seawater fugacity of carbon dioxide (CO2; fCO2SW) was investigated over four seasons in the southeastern Yellow Sea (YS). The seasonal variation in sea surface temperature (SST)-normalized fCO2SW in the study area was largely explained by sea surface concentrations of dissolved inorganic carbon modulated by the water column stability in association with biological carbon fixation and remineralization. Overall, our study area acted as a sink for atmospheric CO2, absorbing a regional average of ~2.8 mmol C m-2 day-1. This result contrasts sharply with the large CO2 effluxes reported in other parts of the YS, implying considerable spatiotemporal variations in fCO2SW in this region. Since the YS is significantly influenced by human activity, our data will serve as a baseline to record the human impact on ocean carbon cycles in the future.

Spatial and vertical distribution of 129I and 127I in the East China Sea: Inventory, source and transportation.

Iodirne-129 is useful for tracking water mass movement in the ocean. In this study, the concentration of iodine isotopes in seawater of the East China Sea (ECS) in October 2013 were analyzed to investigate the spatial and vertical distribution of 129I and 127I to understand water mass exchange. Results showed that the 129I/127I atomic ratios varied with the water mass, with higher values of (10-20) × 10-11 in the coastal regions and lower values of <8 × 10-11 offshore. Inventories of 129I were estimated to be (0.23-1.7) × 1012 atoms m-2 (n = 18) in upper 100 m waters, which is comparable to those of other regions without being contaminated by the nuclear accidents or nuclear reprocessing facilities. The total amount of 129I in the ECS water column was estimated to be 88 g in which over 90% is attributed to the oceanic input (e.g., West Pacific) via the Kuroshio Current (KC). The contributions of 129I from Changjiang (Yangtze River) terrestrial watershed (<7.5%) and atmospheric fallout (<2.7%) were small. Those from the Fukushima accident were negligible during this investigation. The 129I/127I ratios versus salinity distribution showed the range and stratification of the Changjiang, Yellow Sea, and KC waters in the ECS. Our study shows that the Changjiang fresh water could be transported to the North Jiangsu coast in October; the Taiwan Warm Current water could intrude to Northern part of the Changjiang Estuary (32°N). Besides, our results suggest that the 129I/127I profile is useful to indicate the seawater mixing process in ocean marginal systems.

Aragonite saturation state variation and control in the river-dominated marginal BoHai and Yellow seas of China during summer.

Based on a survey conducted from June to July 2013, aragonite saturation state variation and control in the river-dominated marginal BoHai and Yellow seas were investigated. Surface water Ωarag ranged from 2.0-3.8, whereas subsurface water Ωarag was generally lower than 2.0. Temperature changes had a strong influence on Ωarag through induced CO2 solubility changes in seawater. Riverine freshwater input decreased Ωarag in the Changjiang and Yalu river estuaries, but induced higher Ωarag in the Yellow River estuary. Biological processes had opposite effects on Ωarag, whereby elevated biological production led to the highest Ωarag in the South Yellow Sea surface water, whereas net community respiration/remineralization induced low Ωarag in subsurface water. Stratification affected the level and scale of low Ωarag in subsurface water. By the year 2100, surface water with Ωarag > 2.0 will disappear except for the Yellow River estuary, and most of the subsurface water will develop substantial aragonite undersaturation.

Lost but can't be neglected: Huge quantities of small microplastics hide in the South China Sea.

Large quantities of microplastics with small particle sizes were found in the South China Sea (SCS). The abundances of microplastics in seawater were 0.045±0.093and 2569±1770particles/m3 according to the bongo net and pumping sampling methods, respectively. Smaller-size fractions (size<0.3mm) contributed 92% of the number of microplastics to the total load. Continental slope is the largest reservoir of microplastics with an inventory of 295tons. 21 polymer types were found in the samples using the micro Fourier Transform Infrared Spectroscopy (FTIR), among which alkyds (22.5%) and polycaprolactone (PCL) (20.9%) accounted for almost half of the total polymer content. Lighter plastics would not only concentrate upon the coastal area, being more likely to drift further into open seas with ocean currents. The distribution characteristics showed that it was mainly controlled by terrestrial input of the Pearl River. This study, as the first report from SCS on microplastics in water for its distribution and influence factors, provided impetus for further research on the transportation fate and the behavior of this emerging pollutant from coastal zone to the open oceans.

Organic carbon source and salinity shape sediment bacterial composition in two China marginal seas and their major tributaries.

Marginal sea sediments receive organic substrates of different origins, but whether and to what extent sediment microbial communities are reflective of the different sources of organic substrates remain unclear. To address these questions, sediment samples were collected in two connected China marginal seas, i.e., Bohai Sea and Yellow Sea, and their two major tributaries (Yellow River and Liao River). Sediment bacterial community composition (BCC) was examined using 16S rRNA gene pyrosequencing. In addition, physicochemical variables that describe environmental conditions and sediment features were measured. Our results revealed that BCCs changed with salinity and organic carbon (OC) content. Members of Gaiellaceae and Comamonadaceae showed a rapid decrease as salinity and phytoplankton-derived OC increased, while Piscirickettsiaceae and Desulfobulbaceae exhibited an opposite distribution pattern. Differences of riverine vs. marginal sea sediment BCCs could be mostly explained by salinity. However, within the marginal seas, sediment BCC variations were mainly explained by OC-related variables, including terrestrial-derived fatty acids (Terr_FA), phytoplankton-derived polyunsaturated fatty acids (Phyto_PUFA), stable carbon isotopes (δ13C), and carbon to nitrogen ratio (C/N). In addition to environmental variables, network analysis suggested that interactions among individual bacterial taxa might be important in shaping sediment BCCs in the studied areas.