The anti-aging, anti-tuberculosis and antioxidant potential benefits of Saudi Arabia Olea-Europaea Leaves extracts / Potenciais benefícios antienvelhecimento, antituberculose e antioxidantes dos extratos de folhas de Olea-Europaea da Arábia Saudita

The olive leaf extract and olive leaf indicated a high potential for application in food additives and foodstuffs. It could be these bio-products useful and important in condition therapy related with oxidative stress and can use it to develop functional foods and to improve the food's shelf life. The olive leaf chemical composition of Oleaeuropaea L. grown from eljouf in Saudi Arabia, using solvents of increasing polarity cyclohexane, dichloromethane, chloroform, ethyl acetate, methanol and ethanol was determined using by GC/MS. Furthermore, the antioxidant activity (diphenylpicrylhydrazyl (DPPH), anti-aging, and anti-tuberculosis of olive leaf extracts were evaluated. The results indicated that extract of Oleaeuropaea L. has a considerable contains in polyphenols (hydroxytyrosol, oleuropein and their derivatives) regarding its antioxidant effects, the major components were detected by GC/MS in Olea dichloromethane extract are Hexadecanoic acid (15.82%), 7(4Dimethylaminophenyl)3,3,12trimethyl3,12dihydro6 Hpyrano[2,3c]acridin 6 one (11.21%), and in Olea chloroform extract are Hexatriacontane (12.68%), nTetratr iacontane (10.95%). The results concluded that the plant extract of chloroform showed no anti-aging activities and the lower anti-aging activities for cyclohexane extract, while, the Olea dichloromethane extract was the most active extract. The obtained data confirmed that the most active extract of anti-tubercolisis was for chloroform and ethyl acetate extract, while, anti-tubercolisis activity of ethanolic extract was the lower. The extract amount as well as the solvent polarity influence the inhibitory activity. A favorable connection was demonstrated inter alia the leaf extracts antioxidant activity and the content of total phenol.

O extrato de folha de oliveira e a folha de oliveira indicaram alto potencial para aplicação em aditivos alimentares e alimentos. Esses bioprodutos podem ser úteis e importantes na terapia de condições relacionadas ao estresse oxidativo e podem ser utilizados para desenvolver alimentos funcionais e melhorar a vida útil dos alimentos. A composição química da folha de oliveira de Olea europaea L. cultivada em Eljouf na Arábia Saudita, usando solventes de polaridade crescente ciclohexano, diclorometano, acetato de etil clorofórmio, metanol e etanol foi determinada usando GC/MS. Além disso, foi avaliada a atividade antioxidante (difenilpicrilhidrazil - DPPH) antienvelhecimento e antituberculose de extratos de folha de oliveira. Os resultados indicaram que o extrato de Olea europaea L. que consideravelmente possui polifenois (hidroxitirosol, oleuropeína e seus derivados) quanto aos seus efeitos antioxidantes, os componentes majoritários detectados por GC/MS no extrato diclorometânico de Olea são o ácido hexadecanoico (15,82%), 7-(4-Dimetilaminofenil)-3,3,12-trimetil-3,12-dihidro-6H-pirano[2,3c]acridin-6-ona (11,21%) e no extrato de clorofórmio de Olea são Hexatriacontane (12,68%), nTetratr iacontane (10,95%). Os resultados concluíram que o extrato vegetal de clorofórmio não apresentou atividades antienvelhecimento e as atividades antienvelhecimento mais baixas para o extrato de cicloexanona, enquanto o extrato de Olea diclorometano foi o extrato mais ativo. Os dados obtidos confirmaram que o extrato mais ativo de antituberculose foi para clorofórmio e extrato de acetato de etila, enquanto a atividade antituberculose de extrato etanoico foi menor. A quantidade de extrato, bem como a polaridade do solvente influenciam a atividade inibitória, atividade e o teor de fenol total.

Species composition and prevalence of sharpshooters and spittlebugs potential vectors of Xylella fastidiosa in olive orchards of southeastern Brazil

ABSTRACT Xylem sap-feeding auchenorrhynchans, e.g. sharpshooters and spittlebugs (Hemiptera: Cicadellinae and Cercopoidea, respectively), are considered potential vectors of the xylem-limited bacterium Xylella fastidiosa, which is associated with olive leaf desiccation syndrome, a severe disease affecting olive orchards (Olea europaea L.) in the Mantiqueira mountain range region, southeastern Brazil. We evaluated, through faunal analysis, the composition and predominance of Cicadellinae and Cercopoidea species collected fortnightly over two years with yellow sticky cards in eight olive orchards distributed along an altitudinal gradient in the states of São Paulo and Minas Gerais. A high diversity of Cicadellinae (64 species) and Cercopoidea (10 species) was found in the orchards, with 20 of them considered predominant. Clastoptera sp. 1, Macugonalia cavifrons, and Scopogonalia paula were the most representative among the predominant species. We also found out that the trap position on the olive tree canopy at a height of 0.8 m above ground is more efficient than at 1.6 m for capturing the majority of Cicadellinae and Cercopoidea species associated with olive orchards.

Spatial and temporal distribution and bioinsecticides control of olive leaf moth in an olive orchard in Southwest of Paraná, Brazil / Distribuição espacial e temporal e controle com bioinseticidas da lagarta-da-oliveira em um pomar da região Sudoeste do Paraná, Brasil

We verified the spatial and temporal distribution of the olive leaf moth (Palpita forficifera Munroe, 1959) in a new potential region for olive cultivation and evaluated the bioinsecticides effectiveness to control this pest. The experimental orchard composed by Koroneiki and Arbequina cultivars was planted in Pato Branco, Southwest region of Paraná, Brazil. In the field, larvae counts were performed weekly on 25 plants of each cultivar, for 12 months, to verify the spatial and temporal distribution. Furthermore, in laboratory conditions, second instar larvae of P. forficifera were fed with olive leaves treated with Azadirachta indica oil, Bacillus thuringiensis, Metarhizium anisopliae and Beauveria bassiana, and the effectiveness in the larvae mortality was used to compare four bioinsecticides and control. The P. forficifera larvae occurred mainly during the three summer months, had a host preference for the 'Arbequina' (68.9% of total larvae) and occurred at random distribution patterns throughout the orchard. The A. indica oil and B. thuringiensis both caused almost 100% larvae mortality under laboratory conditions.

Os objetivos deste trabalho foram verificar a distribuição espacial e temporal da lagarta-da-oliveira (Palpita forficifera Munroe, 1959) em uma nova região com potencial de cultivo de oliveira e avaliar a eficiência de controle desta praga com bioinseticidas. O pomar experimental com as cultivares Koroneiki e Arbequina foi implantado em Pato Branco, região Sudoeste do Paraná, Brasil. No pomar foram realizadas contagens semanais de lagartas em 25 plantas de cada cultivar, durante 12 meses, para verificar a distribuição espacial e temporal. Além disso, em condições de laboratório, larvas de segundo instar foram submetidas à alimentação com folhas de oliveira tratadas com óleo de neem (Azadirachta indica), Bacillus thuringiensis, Metarhizium anisopliae e Beauveria bassiana, e a efetividade de mortalidade das larvas foi utilizada para comparação entre os quatro bioinseticidas e a testemunha. As lagartas de P. forficifera ocorreram concentradas durante os três meses de verão, tiveram preferência hospedeira pela cultivar Arbequina (68,9% do total de larvas registradas), e ocorreram em padrão de distribuição aleatório ao longo do pomar. A aplicação de óleo de neem (A. indica) ou de B. thuringiensis causaram quase 100% de mortalidade das larvas em condições de laboratório.

Extraction optimization, biological activities, and application in O/W emulsion of deep eutectic solvents-based phenolic extracts from olive pomace.

This study evaluated the phenolic compound extraction from olive pomace with deep eutectic solvents (DES) prepared with choline chloride ([Ch]Cl) and four (poly-)carboxylic acids. Temperature, water addition in the solvent, and solid-liquid ratio were evaluate in total phenolic content and antioxidant activity of extracts obtained with DES and ethanol, as control. Moreover, the antimicrobial activities of solvents and extracts were evaluated. Oil-in-water emulsion with DES extract was prepared, characterized and its oxidative stability analyzed. The extract with the highest total phenolic content was obtained with [Ch]Cl:malonic acid. Under optimal conditions, DES extracted 9 % more total phenolic content than ethanol. DES extract showed superior antibacterial activity to the ethanolic extract, and its presence in oil-in-water emulsion increased the induction time in 10-fold when compared to the one prepared with water. These results reinforce that DES are a potential solvent for phenolic compound extraction from olive pomace with antibacterial and technological benefits.

Microbial biostimulants as alternatives for the rooting of olive tree cuttings

Currently, southern Minas Gerais (MG) state is an important producer of different olive tree (Olea europaea L.) cultivars because, in this region, the plants can differentiate the buds to produce flowers and fruit. To stimulate the rooting of cuttings, the synthetic hormone indole-3-butyric acid (IBA) at a concentration of 3 g L−1 is used commercially. However, few studies have investigated arbuscular mycorrhizal fungi (AMF), isolated or combined with rhizobacteria, as a biotechnological tool to produce hormones that function in the rooting of olive tree cuttings. The aim of this study was to evaluate the capacity of different AMF species (Rhizophagus clarus, Gigaspora rosea, or Acaulospora scrobiculata), combined or not with IBA or rhizobacteria, to promote the rooting of three olive tree cuttings (Arbequina, Grappolo 541, and Maria da Fé) with potential for cultivation in this region. For this, three experiments were conducted at the Experimental Farm of EPAMIG in Maria da Fé (MG), and the rooting potential of the olive tree cuttings inoculated with I) AMF, II) AMF combined with increasing doses of IBA, and III) AMF combined with three isolates of rhizobacteria was evaluated. The inoculation of olive tree cuttings of cultivars Arbequina, Grappolo 541, and Maria da Fé with Rhizophagus clarus, Gigaspora rosea, or Acaulospora scrobiculata combined or not with IBA or rhizobacteria did not significantly promote rooting. Alternative forms of rooting olive tree cuttings are still a challenge, and further studies for standardizing methodologies and experimental conditions are required.

Nocellara Del Belice (Olea europaea L. Cultivar): Leaf Extract Concentrated in Phenolic Compounds and Its Anti-Inflammatory and Radical Scavenging Activity.

Olea europaea L. is a plant belonging to the Oleaceae family, widely grown around the Mediterranean Basin and its leaves are a source of phenolic compounds with antioxidant and anti-inflammatory capacity. Among these, oleuropein and luteolin-7-O-glucoside represent two major polyphenolic compounds in olive-leaf extract. Herein, a polystyrene resin was used to recover the polyphenolic fraction from the acetone-water leaf extract from Nocellara del Belice cultivar, which showed the higher level of analysed bioactive compounds, compared to Carolea cultivar. The antioxidant activity of the extract concentrated in phenolic compounds (OLECp) was evaluated through a classical assay and electron paramagnetic resonance (EPR) for DPPH and hydroxyl radicals scavenging. Thus, the anti-inflammatory activity and the potential beneficial effects in reducing lipid accumulation in an in vitro model of NAFLD using McA-RH7777 cells exposed to oleic acid (OA) were evaluated. Nile Red and Oil Red O have been used to stain the lipid accumulation, while the inflammatory status was assessed by Cytokines Bioplex Assay. OLECp (TPC: 92.93 ± 9.35 mg GAE/g, TFC: 728.12 ± 16.04 mg RE/g; 1 g of extract contains 315.250 mg of oleuropein and 17.44 mg of luteolin-7-O-glucoside) exerted a good radical scavenging capability (IC50: 2.30 ± 0.18 mg/mL) with a neutralizing power against DPPH and hydroxyl radicals, as confirmed by the decreased signal area of the EPR spectra. Moreover, OLECp at concentration of 25, 50 and 100 µg/mL counteracted the intracellular inflammatory status, as result of decreased intracellular lipid content. Our results highlighted the multiple properties and applications of an O. europaea extract concentrated in polyphenols, and the possibility to formulate novel nutraceuticals with antioxidant properties, destined to ameliorate human health.

How Temperatures May Affect the Synthesis of Fatty Acids during Olive Fruit Ripening: Genes at Work in the Field.

A major concern for olive cultivation in many extra-Mediterranean regions is the adaptation of recently introduced cultivars to environmental conditions different from those prevailing in the original area, such as the Mediterranean basin. Some of these cultivars can easily adapt their physiological and biochemical parameters in new agro-environments, whereas others show unbalanced values of oleic acid content. The objective of this study was to evaluate the effects of the thermal regime during oil synthesis on the expression of fatty acid desaturase genes and on the unsaturated fatty acid contents at the field level. Two cultivars (Arbequina and Coratina) were included in the analysis over a wide latitudinal gradient in Argentina. The results suggest that the thermal regime exerts a regulatory effect at the transcriptional level on both OeSAD2 and OeFAD2-2 genes and that this regulation is cultivar-dependent. It was also observed that the accumulated thermal time affects gene expression and the contents of oleic and linoleic acids in cv. Arbequina more than in Coratina. The fatty acid composition of cv. Arbequina is more influenced by the temperature regime than Coratina, suggesting its greater plasticity. Overall, findings from this study may drive future strategies for olive spreading towards areas with different or extreme thermal regimes serve as guidance for the evaluation olive varietal patrimony.

Antimicrobial and cytotoxic activity to human colon adenocarcinoma cell lines (HT-29) potential of olive oil extraction residue.

In the Olive drupe (Olea europaea L.) oil extraction process, 80% of the volume generated is waste (bagasse). Advancing the expansion of the olive oil market, it is necessary to develop alternatives that, in addition to adding value to industrial waste, also reduce possible environmental damage. Our study aimed to understand the antimicrobial and Cytotoxic activity potential of the residues from the extraction of olive oil from the blend of the varieties Arbequina and Arbosana. The extract shows cytotoxic activity, inhibiting about 75% of cancer cells in the human colon at a concentration of 0.15 mg of Gallic Acid equivalent (GAE)/mL. The effectiveness of the extract against microorganisms often associated with foodborne diseases and food decomposition has also been discovered, without compromising the microorganisms responsible for fermentation. Thus, this study provides future perspectives for the use of active ingredients extracted from the residue from the extraction of olive oil.

Choline chloride-based deep eutectic solvents as potential solvent for extraction of phenolic compounds from olive leaves: Extraction optimization and solvent characterization.

This study evaluates the use of deep eutectic solvents (DES) prepared with choline chloride ([Ch]Cl) and carboxylic acids for phenolic compound extraction from olive leaves. These extracts were then compared to those obtained using ethanol. The effects of temperature and water addition during DES- and ethanol-based extractions were analyzed using response surface methodology. Due to the lack of solid-liquid equilibrium (SLE) data for [Ch]Cl + acetic acid, SLE, and DES density and viscosity with and without water addition were measured and analyzed. [Ch]Cl:acetic acid (54.1 °C, 50.0% water addition) extracted 15% more phenolic compounds than ethanol (54.1 °C, 0.5% water addition), according to UHPLC-MS based analyses. SLE analyses showed that [Ch]Cl + acetic acid presented a eutectic region at close to a 1:2 molar ratio. DES precursors and water addition influenced solvent physical properties and phenolic compound yield. DES was confirmed to be an innovative, strong solvent for phenolic compound extraction from olive leaves.

Olive oil characterization of cv. 'Arauco' harvested at different times in areas with early frost in Mendoza, Argentina.

BACKGROUND: 'Arauco' is the only autochthonous olive cultivar from Argentina. Little has been reported so far regarding the management of this crop. In this work, variations in fruit and chemical characteristics of olives harvested over a wide range of dates and seasons are reported for this cultivar at two sites in Mendoza province in central west Argentina. RESULTS: During the harvest periods studied, fruit oil content on a dry basis remained at its maximum and was stable, but fruit oil content on fresh basis increased as water content decreased with delay in harvest date. Harvest date affected the maturity index of fruits as well as the oxidative stability and phenolic content of oil. In contrast, the fatty acid profile was not consistently affected by harvest date. Environmental conditions, mainly the occurrence and intensity of frosts, strongly influenced oil quality as well as maturity with delay in harvest date. CONCLUSION: The most appropriate harvest time to obtain Arauco oil with a high oil yield and good chemical quality was before mid-May and with maturity index lower than 2. Fruits harvested after mid-May were exposed to minimum temperatures between -1.2 °C and - 4.0 °C, producing oil with low phenolic compounds and oxidative stability. © 2019 Society of Chemical Industry.

It Is Feasible to Produce Olive Oil in Temperate Humid Climate Regions.

Worldwide olive industry has expanded into new climatic regions outside the Mediterranean basin due to an increase in extra virgin olive oil demand posing new challenges. This is the case of Uruguay, South America, where the olive crop area reached 10,000 hectares in the last 15 years and is intended to the production of EVOO. Uruguay has a temperate humid climate with mean precipitations above 1,100 mm per year but unequally distributed, mild winters, and warm summers, with mean annual temperatures of 17.7°C. Different agroecological conditions require local knowledge to achieve good productivity whereby the objective of this work was to show the feasibility and potential of olive oil production under our climatic conditions. For this the agronomic performance of Arbequina, Barnea, Frantoio, Leccino, Manzanilla de Sevilla, and Picual cultivars was evaluated along 10 years of full production. Phenology behavior, vegetative growth rate, productive efficiency, alternate bearing, and oil yield were determined. Sprouting and flowering processes occur in a wide window within the annual cycle between the months of August to November with great interannual variation. More than 8 t/ha fruit yield and 40% oil yields in dry weight basis were obtained in promising cultivars. However, alternate bearing arose as the main production limiting factor, with ABI values greater than 0.60 for most cultivars. We conclude that olive oil production in humid climate regions is feasible and the most promising cultivars based on productive efficiency are Arbequina and Picual.

Olive Cultivation in the Southern Hemisphere: Flowering, Water Requirements and Oil Quality Responses to New Crop Environments.

Olive (Olea europaea L.) is a crop well adapted to the environmental conditions prevailing in the Mediterranean Basin. Nevertheless, the increasing international demand for olive oil and table olives in the last two decades has led to expansion of olive cultivation in some countries of the southern hemisphere, notably in Argentina, Chile, Perú and Australia. While the percentage of world production represented by these countries is still low, many of the new production regions do not have typical Mediterranean climates, and some are located at subtropical latitudes where there is relatively little information about crop function. Thus, the primary objective of this review was to assess recently published scientific literature on olive cultivation in these new crop environments. The review focuses on three main aspects: (a) chilling requirements for flowering, (b) water requirements and irrigation management, and (c) environmental effects on fruit oil concentration and quality. In many arid and semiarid regions of South America, temperatures are high and rainfall is low in the winter and early spring months compared to conditions in much of the Mediterranean Basin. High temperatures have often been found to have detrimental effects on olive flowering in many olive cultivars that have been introduced to South America, and a better understanding of chilling requirements is needed. Lack of rainfall in the winter and spring also has resulted in an urgent need to evaluate water requirements from the flower differentiation period in the winter to early fruit bearing. Additionally, in some olive growing areas of South America and Australia, high early season temperatures affect the timing of phenological events such that the onset of oil synthesis occurs sooner than in the Mediterranean Basin with most oil accumulation taking place in the summer when temperatures are very high. Increasing mean daily temperatures have been demonstrated to decrease fruit oil concentration (%) and negatively affect some aspects of oil quality based on both correlative field studies and manipulative experiments. From a practical standpoint, current findings could be used as approximate tools to determine whether the temperature conditions in a proposed new growing region are appropriate for achieving sustainable oil productivity and quality.

Performance of substrates in rooting capacity of olive tree cuttings / Desempenho de substratos na capacidade de enraizamento de estacas de oliveiras

Plant cutting is a widely used propagation method for olive trees. Its viability depends on the capacity for the adventitious root formation of each species. Nevertheless, it is necessary to study the different characteristics of this process. The purpose of this study was to evaluate the influence of substrates on the rooting of semi hardwood stem cuttings of ten olive tree cultivars. Middle sized branches were collected from mother plants located in the Germplasm Bank of EPAMIG in Maria da Fé, MG, Brazil, and cuttings were prepared of approximately 12 cm in length, with 4-6 internodes, maintaining four leaves in the apical region. A completely randomized experimental design was used, with tree replications and 20 cuttings per plot. The experiment was conducted in greenhouses, in rooting plant beds containing a perlite and sand substrate with controlled intermittent water mist. After installation of the experiment, treatment was carried out with copper oxychloride solution at 3% to prevent possible fungus attacks. Evaluations were performed 70 days after cutting of the varieties. There was variation in rooting of olive tree cultivar cuttings. Better performance of sand was observed in the production of cuttings of olive tree cultivars Alto DOuro, JB1 and Galega which were also the cultivars that stood out among the genotypes studied.

A estaquia é um método de propagação muito utilizado para a oliveira. Sua viabilidade depende da capacidade de formação de raízes adventícias de cada espécie. Contudo é necessário estudar as diferentes características desse processo. Objetivouse, no presente trabalho, avaliar a influência dos substratos no enraizamento de estacas semilenhosas de 10 cultivares de oliveira. Ramos medianos foram coletados de plantas matrizes localizadas no Banco de Germoplasma da EPAMIG, em Maria da Fé, MG, e preparadas as estacas com, aproximadamente, 12 cm de comprimento, com 4 a 6 internódios, mantendo na região apical quatro folhas. O delineamento utilizado foi o inteiramente casualizado, com 3 repetições e 20 estacas por parcela. O experimento foi conduzido em casas de vegetação, em canteiros de enraizamento contendo como substrato perlita e areia e nebulização intermitente controlada. Após a instalação do experimento, foi realizado tratamento com solução de oxicloreto de cobre a 3%, a fim de prevenir eventuais ataques de fungos. As avaliações foram realizadas 70 dias após a estaquia das variedades. Existe variação no enraizamento de estacas de cultivares de oliveira. Melhor desempenho da areia foi verificado na estaquia de cultivares de oliveira. Alto DOuro, JB1 e Galega que também foram as cultivares que mais se destacaram entre os genótipos estudados.

Performance of substrates in rooting capacity of olive tree cuttings / Desempenho de substratos na capacidade de enraizamento de estacas de oliveiras

Plant cutting is a widely used propagation method for olive trees. Its viability depends on the capacity for the adventitious root formation of each species. Nevertheless, it is necessary to study the different characteristics of this process. The purpose of this study was to evaluate the influence of substrates on the rooting of semi hardwood stem cuttings of ten olive tree cultivars. Middle sized branches were collected from mother plants located in the Germplasm Bank of EPAMIG in Maria da Fé, MG, Brazil, and cuttings were prepared of approximately 12 cm in length, with 4-6 internodes, maintaining four leaves in the apical region. A completely randomized experimental design was used, with tree replications and 20 cuttings per plot. The experiment was conducted in greenhouses, in rooting plant beds containing a perlite and sand substrate with controlled intermittent water mist. After installation of the experiment, treatment was carried out with copper oxychloride solution at 3% to prevent possible fungus attacks. Evaluations were performed 70 days after cutting of the varieties. There was variation in rooting of olive tree cultivar cuttings. Better performance of sand was observed in the production of cuttings of olive tree cultivars Alto DOuro, JB1 and Galega which were also the cultivars that stood out among the genotypes studied.(AU)

A estaquia é um método de propagação muito utilizado para a oliveira. Sua viabilidade depende da capacidade de formação de raízes adventícias de cada espécie. Contudo é necessário estudar as diferentes características desse processo. Objetivouse, no presente trabalho, avaliar a influência dos substratos no enraizamento de estacas semilenhosas de 10 cultivares de oliveira. Ramos medianos foram coletados de plantas matrizes localizadas no Banco de Germoplasma da EPAMIG, em Maria da Fé, MG, e preparadas as estacas com, aproximadamente, 12 cm de comprimento, com 4 a 6 internódios, mantendo na região apical quatro folhas. O delineamento utilizado foi o inteiramente casualizado, com 3 repetições e 20 estacas por parcela. O experimento foi conduzido em casas de vegetação, em canteiros de enraizamento contendo como substrato perlita e areia e nebulização intermitente controlada. Após a instalação do experimento, foi realizado tratamento com solução de oxicloreto de cobre a 3%, a fim de prevenir eventuais ataques de fungos. As avaliações foram realizadas 70 dias após a estaquia das variedades. Existe variação no enraizamento de estacas de cultivares de oliveira. Melhor desempenho da areia foi verificado na estaquia de cultivares de oliveira. Alto DOuro, JB1 e Galega que também foram as cultivares que mais se destacaram entre os genótipos estudados.(AU)

Influence of agroclimatic parameters on phenolic and volatile compounds of Chilean virgin olive oils and characterization based on geographical origin, cultivar and ripening stage.

BACKGROUND: This study involved two commercial orchards located in Limarí Valley and Molina from two important Chilean production zones of extra virgin olive oil (EVOO). The investigation evaluated the effects of climate, soil composition, agricultural practices (fertilization and irrigation) and variety (considering two harvests) on the compounds responsible for the flavor of EVOO (volatiles and phenols) and how these compounds can explain the differences in chemical profiles by geographical origin, cultivar and fruit ripeness stage. RESULTS: Varieties from the Limarí Valley presented the highest content of phenolic compounds. A significant relationship (P < 0.05) between volatile compounds and climate indicated that the compounds produced via the lipoxygenase cascade were affected by the maximum temperature and, to a lesser extent, by evapo-transpiration and irrigation. The selection of different individual phenolic and volatile compounds independently allowed the significant differentiation of EVOOs, principally by geographical origin, crop season, fruit ripeness stage and, in a few cases, by cultivar. CONCLUSION: Soil and climate of the Chilean regions have much more influence than cultivars on the concentration of sensory quality compounds. Difference in latitude between orchards increases the importance of the geographical origin on the virgin olive oil chemical composition while full irrigation decreases the impact of the cultivar.

Desenvolvimento vegetativo de duas cultivares de oliveira na fase de muda / Vegetative development of two olive cultivars in seedling phase

O desenvolvimento de plantas, influenciado pela temperatura do ar, pode ser estimado através do conceito de filocrono, definido como o intervalo de tempo entre o surgimento sucessivo de folhas em uma haste, sendo o tempo expresso em °C dia e o filocrono em °C dia folha-1. O objetivo deste estudo foi avaliar o desenvolvimento de duas cultivares de oliveira durante a fase de muda, em diferentes épocas de aclimatação, utilizando o conceito de filocrono. O experimento foi conduzido na Fazenda Experimental da Epamig, em Maria da Fé, sul de Minas Gerais, Brasil. O delineamento utilizado foi o inteiramente casualizado em esquema fatorial 2x5, com duas cultivares ('MGS ASC315' e 'Arbequina'), cinco épocas de aclimatação (maio, junho, julho, agosto e setembro de 2010) e 15 repetições. O filocrono foi estimado pelo inverso do coeficiente angular da regressão linear entre o número de folhas emitidas (NF) e a soma térmica acumulada (STa). O filocrono em 'MGS ASC315' foi de 14,7°C dia folha-1 (época 1), 31,7°C dia folha-1 (média das épocas 2, 3 e 4) e 60,2°C dia folha-1 (época 5). O filocrono em 'Arbequina' foi de 17,3°C dia folha-1 (média das cinco épocas de aclimatação, uma vez que não apresentou diferença significativa entre as épocas). Nas épocas de aclimatação 3, 4 e 5, houve diferença do filocrono entre as cultivares, sendo que o menor filocrono foi para a cultivar 'Arbequina'. Nas épocas 1 e 2, não houve diferenças significativas entre as cultivares.

Plants development, influenced by air temperature, can be estimated through the concept of phyllochron, defined as the time interval between the appearance of successive leaves on a stem, being the time expressed in °C day and the phyllochron in °C day leaf-1. The objective of this study was to evaluate the development of two olive cultivars during the seedling phase at different acclimation dates, using the concept of phyllochron. The experiment was carried in the experimental area of the Agricultural Research Corporation of Minas Gerais, Maria da Fé, south of Minas Gerais, Brazil. The experimental design was completely randomized with two cultivars (MGS 'ASC315' and 'Arbequina'), five acclimation date (May, June, July, August and September 2010) and 15 repetitions. The phyllochron was estimated by the inverse of the slope of the linear regression between leaves number (NF) and accumulated thermal time (STa). The phyllochron in 'MGS ASC315' was 14,7°C day leaf-1 (date 1), 31,7°C day leaf-1 (average of dates 2, 3 and 4) and 60,2°C day leaf-1(date 5). The phyllochron in 'Arbequina' was 17,3°C day leaf-1 (average of five acclimation dates, due not statistically difference among dates). In acclimation dates 3, 4 and 5, not statistically difference between cultivars, and the smallest phyllochron for 'Arbequina'. In acclimation dates 1 and 2, were not statistically different between cultivars.

Assessment of inter- and intra-cultivar variations in olive using SSR markers

Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this high level of genetic variation is probably due to the location of Turkey in the center of the origin of olive. The UPGMA dendrogram, developed to visualize the estimated genetic relationships among the 135 samples, demonstrated that the clustering of olive cultivars was not based on geographical regions of cultivation. Presence of genetic variation was detected within a nationwide grown Turkish olive cultivar, called 'Gemlik'. Olive growers successfully discriminated olive cultivars with distinct morphological and pomological characters. However, there was some confusion about the identification of cultivars with similar phenotypic traits. To prevent misidentification of olive cultivars and to minimize intra-cultivar variation, certified propagation materials which were characterized using DNA based molecular markers should be used during the establishment of new olive orchards.

Assessment of inter- and intra-cultivar variations in olive using SSR markers

Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this high level of genetic variation is probably due to the location of Turkey in the center of the origin of olive. The UPGMA dendrogram, developed to visualize the estimated genetic relationships among the 135 samples, demonstrated that the clustering of olive cultivars was not based on geographical regions of cultivation. Presence of genetic variation was detected within a nationwide grown Turkish olive cultivar, called 'Gemlik'. Olive growers successfully discriminated olive cultivars with distinct morphological and pomological characters. However, there was some confusion about the identification of cultivars with similar phenotypic traits. To prevent misidentification of olive cultivars and to minimize intra-cultivar variation, certified propagation materials which were characterized using DNA based molecular markers should be used during the establishment of new olive orchards.

Desenvolvimento vegetativo de duas cultivares de oliveira na fase de muda

Plants development, influenced by air temperature, can be estimated through the concept of phyllochron, defined as the time interval between the appearance of successive leaves on a stem, being the time expressed in °C day and the phyllochron in °C day leaf-1. The objective of this study was to evaluate the development of two olive cultivars during the seedling phase at different acclimation dates, using the concept of phyllochron. The experiment was carried in the experimental area of the Agricultural Research Corporation of Minas Gerais, Maria da Fé, south of Minas Gerais, Brazil. The experimental design was completely randomized with two cultivars (MGS 'ASC315' and 'Arbequina'), five acclimation date (May, June, July, August and September 2010) and 15 repetitions. The phyllochron was estimated by the inverse of the slope of the linear regression between leaves number (NF) and accumulated thermal time (STa). The phyllochron in 'MGS ASC315' was 14,7°C day leaf-1 (date 1), 31,7°C day leaf-1 (average of dates 2, 3 and 4) and 60,2°C day leaf-1(date 5). The phyllochron in 'Arbequina' was 17,3°C day leaf-1 (average of five acclimation dates, due not statistically difference among dates). In acclimation dates 3, 4 and 5, not statistically difference between cultivars, and the smallest phyllochron for 'Arbequina'. In acclimation dates 1 and 2, were not statistically different between cultivars.

O desenvolvimento de plantas, influenciado pela temperatura do ar, pode ser estimado através do conceito de filocrono, definido como o intervalo de tempo entre o surgimento sucessivo de folhas em uma haste, sendo o tempo expresso em °C dia e o filocrono em °C dia folha-1. O objetivo deste estudo foi avaliar o desenvolvimento de duas cultivares de oliveira durante a fase de muda, em diferentes épocas de aclimatação, utilizando o conceito de filocrono. O experimento foi conduzido na Fazenda Experimental da Epamig, em Maria da Fé, sul de Minas Gerais, Brasil. O delineamento utilizado foi o inteiramente casualizado em esquema fatorial 2x5, com duas cultivares ('MGS ASC315' e 'Arbequina'), cinco épocas de aclimatação (maio, junho, julho, agosto e setembro de 2010) e 15 repetições. O filocrono foi estimado pelo inverso do coeficiente angular da regressão linear entre o número de folhas emitidas (NF) e a soma térmica acumulada (STa). O filocrono em 'MGS ASC315' foi de 14,7°C dia folha-1 (época 1), 31,7°C dia folha-1 (média das épocas 2, 3 e 4) e 60,2°C dia folha-1 (época 5). O filocrono em 'Arbequina' foi de 17,3°C dia folha-1 (média das cinco épocas de aclimatação, uma vez que não apresentou diferença significativa entre as épocas). Nas épocas de aclimatação 3, 4 e 5, houve diferença do filocrono entre as cultivares, sendo que o menor filocrono foi para a cultivar 'Arbequina'. Nas épocas 1 e 2, não houve diferenças significativas entre as cultivares.

Desenvolvimento vegetativo de duas cultivares de oliveira na fase de muda

Plants development, influenced by air temperature, can be estimated through the concept of phyllochron, defined as the time interval between the appearance of successive leaves on a stem, being the time expressed in °C day and the phyllochron in °C day leaf-1. The objective of this study was to evaluate the development of two olive cultivars during the seedling phase at different acclimation dates, using the concept of phyllochron. The experiment was carried in the experimental area of the Agricultural Research Corporation of Minas Gerais, Maria da Fé, south of Minas Gerais, Brazil. The experimental design was completely randomized with two cultivars (MGS 'ASC315' and 'Arbequina'), five acclimation date (May, June, July, August and September 2010) and 15 repetitions. The phyllochron was estimated by the inverse of the slope of the linear regression between leaves number (NF) and accumulated thermal time (STa). The phyllochron in 'MGS ASC315' was 14,7°C day leaf-1 (date 1), 31,7°C day leaf-1 (average of dates 2, 3 and 4) and 60,2°C day leaf-1(date 5). The phyllochron in 'Arbequina' was 17,3°C day leaf-1 (average of five acclimation dates, due not statistically difference among dates). In acclimation dates 3, 4 and 5, not statistically difference between cultivars, and the smallest phyllochron for 'Arbequina'. In acclimation dates 1 and 2, were not statistically different between cultivars.

O desenvolvimento de plantas, influenciado pela temperatura do ar, pode ser estimado através do conceito de filocrono, definido como o intervalo de tempo entre o surgimento sucessivo de folhas em uma haste, sendo o tempo expresso em °C dia e o filocrono em °C dia folha-1. O objetivo deste estudo foi avaliar o desenvolvimento de duas cultivares de oliveira durante a fase de muda, em diferentes épocas de aclimatação, utilizando o conceito de filocrono. O experimento foi conduzido na Fazenda Experimental da Epamig, em Maria da Fé, sul de Minas Gerais, Brasil. O delineamento utilizado foi o inteiramente casualizado em esquema fatorial 2x5, com duas cultivares ('MGS ASC315' e 'Arbequina'), cinco épocas de aclimatação (maio, junho, julho, agosto e setembro de 2010) e 15 repetições. O filocrono foi estimado pelo inverso do coeficiente angular da regressão linear entre o número de folhas emitidas (NF) e a soma térmica acumulada (STa). O filocrono em 'MGS ASC315' foi de 14,7°C dia folha-1 (época 1), 31,7°C dia folha-1 (média das épocas 2, 3 e 4) e 60,2°C dia folha-1 (época 5). O filocrono em 'Arbequina' foi de 17,3°C dia folha-1 (média das cinco épocas de aclimatação, uma vez que não apresentou diferença significativa entre as épocas). Nas épocas de aclimatação 3, 4 e 5, houve diferença do filocrono entre as cultivares, sendo que o menor filocrono foi para a cultivar 'Arbequina'. Nas épocas 1 e 2, não houve diferenças significativas entre as cultivares.