Phytoremediation Potential of Crotalaria pumila (Fabaceae) in Soils Polluted with Heavy Metals: Evidence from Field and Controlled Experiments.

Phytoremediation is a useful, low-cost, and environmentally friendly alternative for the rehabilitation of heavy-metal-contaminated (HM) soils. This technology takes advantage of the ability of certain plant species to accumulate HMs in their tissues. Crotalaria pumila is a herbaceous plant with a wide geographical distribution that grows naturally in environments polluted with HMs. In this work, the bioaccumulation capacity of roots and leaves in relation to five HMs (Cr, Cu, Fe, Pb, and Zn) was evaluated, as well as the morphological changes presented in C. pumila growing in control substrate (without HMs) and mine-tailing substrate (with HMs) under greenhouse conditions for 150 days. Four metals with the following concentration pattern were detected in both tissues and substrates: Fe > Pb > Cu > Zn. Fe, Pb, and Zn concentrations were significantly higher in the roots and leaves of individuals growing on mine-tailing substrate compared to the control substrate. In contrast, Cu concentration increased over time in the exposed individuals. The bioconcentration factor showed a similar pattern in root and leaf: Cu > Fe > Pb > Zn. Around 87.5% of the morphological characters evaluated in this species decreased significantly in individuals exposed to HMs. The bioconcentration factor shows that C. pumila is efficient at absorbing Cu, Fe, and Pb from the mine-tailing substrate, in the root and leaf tissue, and the translocation factor shows its efficiency in translocating Cu from the roots to the leaves. Therefore, C. pumila may be considered as a HM accumulator plant with potential for phytoremediation of polluted soils with Cu, Pb, and Fe, along with the ability to establish itself naturally in contaminated environments, without affecting its germination rates. Also, it exhibits wide geographical distribution, it has a short life cycle, exhibits rapid growth, and can retain the mine-tailing substrate, extracting HMs in a short time.

Potential of Pinus radiata, Eucalyptus globulus and Acacia dealbata for the long-term phytostabilization of copper mine tailings.

Many contaminated tailings throughout the world cause environmental and human-health related problems due to air and water drift. Tailing phytostabilization is a promising solution, but only certain plant species may tolerate and grow in these contaminated areas. We analyzed the chemical properties of a vegetated and unvegetated area in a tailing site in Central Chile. In addition, in the vegetated area we analyzed the metals content of roots, stems, and foliage in 41-years old plantations of Pinus radiata, Acacia dealbata, and Eucalyptus globulus (the only three species that survived from a total of 34 species planted), and determined height (H), and diameter at breast height (DBH). The results indicated that, except for pH, Se, Pb, and organic matter, all components (nutrients and metals) were two- to three- fold lower in the vegetated tailing compared to that of the unvegetated tailing. The analysis of plant tissues indicated that Cu was higher in the roots of P. radiata (2,073 mg kg-1) and lower in the stems of the same species (4.1 mg kg-1). However, the ability to take up and transport Cu to the shoots was higher in A. dealbata and lower in P. radiata (bioaccumulation factor of 0.19 and 0.06, respectively).

Here we present results for the first long-term phytostabilization project of copper mine tailings in Chile. From the 34 native and exotic species established in 1980 in a mine tailing disposal site with 1,000 mg Cu kg⁻¹, only the exotic Pinus radiata, Acacia dealbata and Eucalyptus globulus were able to survive and adapt to the tailing conditions the last 41 years. This corroborates their potential for the future phytostabilization of copper mine wastes.

Beneficial Interactive Effects Provided by an Arbuscular Mycorrhizal Fungi and Yeast on the Growth of Oenothera picensis Established on Cu Mine Tailings.

Phytoremediation, an environmentally friendly and sustainable approach for addressing Cu-contaminated environments, remains underutilized in mine tailings. Arbuscular mycorrhizal fungi (AMF) play a vital role in reducing Cu levels in plants through various mechanisms, including glomalin stabilization, immobilization within fungal structures, and enhancing plant tolerance to oxidative stress. Yeasts also contribute to plant growth and metal tolerance by producing phytohormones, solubilizing phosphates, generating exopolysaccharides, and facilitating AMF colonization. This study aimed to assess the impact of AMF and yeast inoculation on the growth and antioxidant response of Oenothera picensis plants growing in Cu mine tailings amended with compost. Plants were either non-inoculated (NY) or inoculated with Meyerozyma guilliermondii (MG), Rhodotorula mucilaginosa (RM), or a combination of both (MIX). Plants were also inoculated with Claroideoglomus claroideum (CC), while others remained non-AMF inoculated (NM). The results indicated significantly higher shoot biomass in the MG-NM treatment, showing a 3.4-fold increase compared to the NY-NM treatment. The MG-CC treatment exhibited the most substantial increase in root biomass, reaching 5-fold that in the NY-NM treatment. Co-inoculation of AMF and yeast influenced antioxidant activity, particularly catalase and ascorbate peroxidase. Furthermore, AMF and yeast inoculation individually led to a 2-fold decrease in total phenols in the roots. Yeast inoculation notably reduced non-enzymatic antioxidant activity in the ABTS and CUPRAC assays. Both AMF and yeast inoculation promoted the production of photosynthetic pigments, further emphasizing their importance in phytoremediation programs for mine tailings.

Root development in Leucaena leucocephala (Lam.) de Wit enhances copper accumulation.

Potentially toxic elements (PTE) in soil like copper (Cu) have been common in agricultural and mining areas worldwide. The sustainable remediation of these areas has been shown to have high socio-environmental relevance and phytoremediation is one of the green technologies to be considered. The challenge is to identify species that are tolerant to PTE, and to assess their phytoremediation potential. The objective of this study was to evaluate the physiological response of Leucaena leucocephala (Lam.) de Wit and to determine the species tolerance and phytoremediation potential to concentrations of Cu in the soil (100, 200, 300, 400 and 500 mg/dm3). The photosynthetic rate was not affected, while the content of chlorophylls decreased as Cu concentrations increased. There was an increased in stomatal conductance and water use efficiency from the treatment of 300. The root biomass and the length were bigger than the shoots, in the treatments above 300. Cu accumulation was greater in the roots than in the shoot of the plants, thus, the Cu translocation index to the shoot was lower. The ability to absorb and accumulate, mainly, Cu in the roots, allowed the development and growth of plants, since the parameters of photosynthesis and biomass accumulation were not affected by the Cu excess. This accumulation in the roots is characterized as a strategy for the phytostabilization of Cu. Therefore, L. leucocephala is tolerant to the Cu concentrations evaluated and has a potential phytoremediation of Cu in the soil.

Organic fertilization and mycorrhization increase copper phytoremediation by Canavalia ensiformis in a sandy soil.

Organic fertilization and mycorrhization can increase the phytoremediation of copper-contaminated soils. The time of vermicomposting alters the properties of vermicompost, which can affect copper's availability and uptake. Therefore, this study sought to evaluate the effect of different organic fertilizers and mycorrhization on copper-contaminated soil phytoremediation. The soil was contaminated with 100 mg Cu kg-1 dry soil and received mineral fertilizer (MIN), bovine manure (CM), and vermicompost produced in 45 days (V45) or 120 days (V120), all in doses equivalent to 40 mg kg-1 dry soil of phosphorus. Half of the jack bean (Canavalia ensiformis) plants were inoculated with the arbuscular mycorrhizal fungus Rhizophagus clarus. At plant flowering, the dry mass and concentrations of Cu, Zn, Mn, Ca, Mg, P, and K in the soil, solution, and plant tissue were determined, in addition to mycorrhizal colonization, nodulation, photosynthetic pigments, and oxidative stress enzyme activity. Organic fertilization increased plant growth and copper accumulation in aerial tissues. These effects were more evident with the V120, making it suitable for use in copper phytoextraction. Mycorrhization increased root and nodule dry mass, making it recommended for phytostabilization. C. ensiformis nodulation in Cu-contaminated soils depends on vermicompost fertilization and mycorrhization. Hence, the copper phytoremediation by C. ensiformis is increased by using organic fertilization and mycorrhization.

Phytostabilization of trace elements and 13C isotope composition of Atriplex atacamensis Phil. cultivated in mine tailings treated with organic amendments.

Mining generates large quantities of mineral processing wastes that are typically stored in mine tailings (MT) ponds. Long-term exposure of the surrounding areas to the material from the tailings ponds has been reported to have adverse effects on both human health and the environment. The purpose of this study was to evaluate the ability of Atriplex atacamensis Phil. to phytostabilize metals (Cu, Fe, Mn, and Zn) and sulfur (S) when grown directly on mine tailings with and without compost (C) and humic substance (HS). The stress status of A. atacamensis Phil. was also evaluated through the 13C isotopic composition of bulk leaves. A 120-day greenhouse experiment was conducted and three treatments were evaluated: (i) MT without any amendments (control), (ii) MT + C (dose: 89 ton ha-1), and (iii) MT + HS (0.72 ton ha-1). Mine tailings material exhibited low salinity, alkaline pH, high extractable S-SO4 concentrations, and low fertility; total Fe, Mn, and Zn concentrations were within the reference range for mine tailings, but total Cu concentrations were high at 1860 ± 236 mg kg-1. The HS had higher pH, EC, CEC, and available concentrations of N, P, and K than compost, while S-SO4 concentrations were similar in both amendments. 13C NMR analysis showed that the HS contained more alkyl, aromatic, and phenolic groups, while the compost was dominated by O-alkyl and carboxyl groups. At the end of the experiment, the MT + C treatment achieved a significant decrease in Cu, Fe, and Mn concentrations in the roots and aboveground parts of A. atacamensis Phil. and an increase in Zn values in both tissues. Both amendments increased the sulfur content in the aboveground parts, while metal concentrations under the HS treatment proved similar to control. Furthermore, the δ13CV-PDB values obtained in this study indicate that the organic amendments did not cause additional physiological stress to the plants compared to the MT treatment. Overall, A. atacamensis Phil. was shown to have the ability to phytostabilize metals and sulfur, making it a potential candidate species for in situ evaluation of the phytostabilization process on mine tailings.

Assisted Phytostabilization of Mine-Tailings with Prosopis laevigata (Fabaceae) and Biochar.

Phytoremediation is a cost-effective technique to remediate heavy metal (HM) polluted sites. However, the toxic effects of HM can limit plant establishment and development, reducing phytoremediation effectiveness. Therefore, the addition of organic amendments to mine wastes, such as biochar, improves the establishment of plants and reduces the bioavailability of toxic HM and its subsequent absorption by plants. Prosopis laevigata can establish naturally in mine tailings and accumulate different HM; however, these individuals show morphological and genetic damage. In this study, the effect of biochar on HM bioaccumulation in roots and aerial tissues, HM translocation, morphological characters and plant growth were evaluated, after three and six months of exposure. Plants grown on mine tailings with biochar presented significantly higher values for most of the evaluated characters, in respect to plants that grew on mine tailing substrate. Biochar addition reduced the bioaccumulation and translocation of Cu, Pb, and Cd, while it favored the translocation of essential metals such as Fe and Mn. The addition of biochar from agro-industrial residues to mine tailings improves the establishment of plants with potential to phytoextract and phytostabilize metals from polluted soils. Using biochar and heavy metal accumulating plants constitutes an assisted phytostabilization strategy with great potential for HM polluted sites such as Cd and Pb.

Phytomanagement of a Lead-Polluted Shooting Range Using an Aromatic Plant Species and Its Effects on the Rhizosphere Bacterial Diversity and Essential Oil Production.

This field study aimed to assess the baseline conditions of a long-term shooting range in Argentina polluted with 428 mg kg-1 lead (Pb) to evaluate the establishment and development of Helianthus petiolaris plants and address the efficacy of the phytomanagement strategy through: (i) element accumulation in plant tissues; (ii) rhizosphere bacterial diversity changes by Illumina Miseq™, and (iii) floral water and essential oil yield, composition, and element concentration by GC-MS and ICP. After one life cycle growing in the polluted sites, in the roots of Helianthus petiolaris plants, Pb concentration was between 195 and 304 mg kg-1 Pb. Only a limited fraction of the Pb was translocated to the aerial parts. The predominance of the genus Serratia in the rhizosphere of Helianthus petiolaris plants cultivated in the polluted sites and the decrease in the essential oil yield were some effects significantly associated with soil Pb concentration. No detectable Pb concentration was found in the floral water and essential oil obtained. Extractable Pb concentration in the soil reduced between 28% and 45% after the harvest.

Phytoremediation of Soils Contaminated with Heavy Metals from Gold Mining Activities Using Clidemia sericea D. Don.

Soils contaminated by potentially toxic elements (PTEs) as a result of anthropogenic activities such as mining are a problem due to the adverse effects on human and environmental health, making it necessary to seek sustainable strategies to remediate contaminated areas. The objective of this study was to evaluate the species Clidemia sericea D. Don for the phytoremediation of soils contaminated with PTEs (Hg, Pb, and Cd) from gold mining activities. The study was conducted for three months, with soils from a gold mining area in northern Colombia, and seeds of C. sericea, under a completely randomized experimental design with one factor (concentration of PTEs in soil) and four levels (control (T0), low (T1), medium (T2), and high (T3)), each treatment in triplicate, for a total of twelve experimental units. Phytotoxic effects on plants, bioconcentration (BCF), and translocation (TF) factors were determined. The results obtained for the tissues differed in order of metal accumulation, with the root showing the highest concentration of metals. The highest values of bioconcentration (BCF > 1) were presented for Hg at T3 and Cd in the four treatments; and of translocation (TF > 1) for Hg and Pb at T0 and T1; however, for Pb, the TF indicates that it is transferable, but it is not considered for phytoextraction. Thus, C. sericea demonstrated its potential as a phytostabilizer of Hg and Cd in mining soils, strengthening as a wild species with results of resistance to the stress of the PTEs evaluated, presenting similar behavior and little phytotoxic affectation on the growth and development of each of the plants in the different treatments.

Hydrothermally-altered feldspar reduces metal toxicity and promotes plant growth in highly metal-contaminated soils.

Novel green technologies for soil remediation have been focusing on altering soil properties and improving soil health. Hydrothermally-altered feldspar (HYP, HydroPotash), recently developed, is being related as both an efficient amendment to immobilize heavy metals in soils and a plant nutrients source, consisting in a promising technology for revegetation of contaminated sites. In order to evaluate the effectiveness of using HYP for phytostabilization programs, two different soils (Technosol and Oxisol) collected from a smelting site were amended with increasing doses of HYPs (HYP-1 and HYP-2): 15, 30, 60, and 120 Mg ha-1. For comparison, a control (soil without amendment) and a soil amended with zeolite (clinoptilolite) were also included as treatments. After 90 days of incubation, HYPs decreased up to 83.8 % of Cd availability and reduced exchangeable Al up to 100 %. HydroPotash increased pH, cation exchange capacity, and contents of potassium, calcium, and phosphorus, as well as microbial biomass carbon, and fluorescein diacetate hydrolysis of soils. Andropogon gayanus, Eucalyptus grandis, and Heterocondylus vitalbae started growing from the dose of 15 Mg ha-1 HYPs in the Oxisol and 60 Mg ha-1 HYPs in the Technosol. Principal component analysis indicates that plant shoot dry weight was negatively correlated with extractable Cd and Zn and positively with pH, CEC, and Ca content. Besides promoting plant growth, HYPs reduced heavy metals (Cd and Zn) absorption by plants, indicating that HYP has potential use as an amendment in phytostabilization programs.

Phytoremediation of metals by colonizing plants developed in point bars in the channeled bed of the Dilúvio Stream, Southern Brazil.

Urban rivers are intensely impacted by pollution with metals resulting from anthropogenic activities, and these elements present in water and sediments can be ecofriendly phytoremediated. This study aimed to evaluate the levels of metals in the sediments and colonizing plants growing in point bars in the channeled bed of the Dilúvio Stream, Southern Brazil. Sediment and plants were sampled at five-point bars with consolidated vegetation. These point bars are formed mainly by sand, with increasing concentrations of clay plus silt, carbon, nitrogen, and metals (Zn, Cu, Cr, Ni, Pb, and Cd) downstream. The concentration of Zn (338 µg/g) and Cu (219 µg/g) in sediments were 1.6 and 1.11 above the probable effect level at the most downstream site. The translocation factor was low in all sites and for all potentially toxic metals evaluated (ranging from 0.01 to 0.63). However, bioaccumulation factor exhibited high values, especially for Cd (average of 2.51), Ni (1.62), Zn (1.49), and Cr (1.25), suggesting that the colonizing plants have more potential for phytostabilization and phytoaccumulation than phytoextraction. These plants can be considered as natural filtering reducing the environmental contamination and the flow of these contaminants in the drainage network. Statement of novelty: Colonizing plants growing in point bars of urban rivers are common around the world; however, their phytoremediation potential is poorly studied. Colonizing plants may be useful for phytoremediation of water, effluents, and sediments of the Dilúvio Stream (Southern Brazil), polluted by potentially toxic metals that originated from the urbanization.

Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review.

The pollution of soil, water, and air by potentially toxic trace elements poses risks to environmental and human health. For this reason, many chemical, physical, and biological processes of remediation have been developed to reduce the (available) trace element concentrations in the environment. Among those technologies, phytoremediation is an environmentally friendly in situ and cost-effective approach to remediate sites with low-to-moderate pollution with trace elements. However, not all species have the potential to be used for phytoremediation of trace element-polluted sites due to their morpho-physiological characteristics and low tolerance to toxicity induced by the trace elements. Grasses are prospective candidates due to their high biomass yields, fast growth, adaptations to infertile soils, and successive shoot regrowth after harvest. A large number of studies evaluating the processes related to the uptake, transport, accumulation, and toxicity of trace elements in grasses assessed for phytoremediation have been conducted. The aim of this review is (i) to synthesize the available information on the mechanisms involved in uptake, transport, accumulation, toxicity, and tolerance to trace elements in grasses; (ii) to identify suitable grasses for trace element phytoextraction, phytostabilization, and phytofiltration; (iii) to describe the main strategies used to improve trace element phytoremediation efficiency by grasses; and (iv) to point out the advantages, disadvantages, and perspectives for the use of grasses for phytoremediation of trace element-polluted soils.

Tolerance of Mentha crispa L. (garden mint) cultivated in cadmium-contaminated oxisol.

The tolerance of Mentha crispa L. (garden mint) cultivated in cadmium-contaminated oxisol for 120 days was analyzed using plant growth variables such as height, the number of leaves and shoots in different Cd exposure periods, as well as assessing the metal concentration absorbed and accumulated in the plant parts (root, stem, and leaves). The maximum adsorption capacity was estimated at 9220 mg kg-1 and used as a reference to establish the different Cd concentrations to be applied in the soil. M. crispa showed tolerance and revealed a reduction of height, the number of leaves and shoots, root development, and secondary toxicity signs such as chlorosis and leaf wilting. Comparing to the stems and leaves, Cd was retained mainly in the roots. PERMANOVA showed that plant growth variables and Cd concentrations in the plant's part were affected by the Cd exposure time. The canonical discriminant analysis demonstrated height as the most affected variable until 45 growing days, and different responses were observed after 75 days. However, the number of shoots was the variable most affected by higher Cd concentrations. The bioaccumulation and translocation factors for all treatments were lower than one, indicating that M. crispa can be considered as an excluder plant and applied for a phytostabilization strategy.

Metagenomics of mine tailing rhizospheric communities and its selection for plant establishment towards bioremediation.

Mining operations often generate tailing dams that contain toxic residues and are a source of contamination when left unconfined. The establishment of a plant community over the tailings has been proposed as a containment strategy known as phytostabilization. Previously, we described naturally occurring mine tailing colonizing plants such as Acacia farnesiana, Brickellia coulteri, Baccharis sarothroides, and Gnaphalium leucocephalum without finding local adaptation. We explored the rhizosphere microbes as contributors in plant establishment and described both the culturable and in situ diversity of rhizospheric bacteria using the 16S rRNA gene and metagenomic shotgun sequencing. We built a synthetic community (SC) of culturable rhizosphere bacteria from the mine tailings. The SC was then the foundation for a serial passes experiment grown in plant-derived nutrient sources, selecting for heavy metals tolerance, community cooperation, and competition. The outcome of the serial passes was named the 'final synthetic community' (FSC). Overall, diversity decreased from in situ uncultivable microbes from roots (399 bacteria genera) to the cultivated communities (291 genera), the SC (94 genera), and the lowest diversity was in the FSC (43 genera). Metagenomic diversity clustered into 94,245 protein families, where we found plant growth promotion-related genes such as the csgBAC and entCEBAH, coded in a metagenome-assembled genome named Kosakonia sp. Nacozari. Finally, we used the FSC to inoculate mine tailing colonizing plants in a greenhouse experiment. The plants with the FSC inocula observed higher relative plant growth rates in sterile substrates. The FSC presents promising features that might make it useful for phytostabilization tailored strategies.

Vicia villosa Roth: a cover crop to phytoremediate arsenic polluted environments.

Vicia villosa Roth is a legume species with a growing application in Argentina as a cover crop (CC), a practice that favors the sustainable development of agricultural systems. However, several areas where the use of this CC provides numerous advantages are affected by high concentrations of arsenic (As). Thus, in the present work we studied hairy vetch ability to cope with arsenate [As(V)], arsenite [As(III)], and the mixture of both along with oxidative stress indexes [chlorophyll content, malondialdehyde (MDA) equivalents] as well as anatomical and histological changes in the root structure. The results obtained suggested a different behavior of hairy vetch depending on its growth stage and on metal(oid) concentration. The roots treated with the contaminant showed less turgidity, thickening of the epidermal and subepidermal parenchymal outer layers, and the presence of dark deposits. The morpho-anatomic parameters (cortex length, vascular cylinder diameter, total diameter, and vascular cylinder area) were altered in plants treated with As(V) and As(V)/As(III) whereas the roots of plants treated with As(III) did not show significant differences respect to the control. Moreover V. villosa could tolerate and remove As from soil, thus the use of this legume species seems an attractive approach to remediate As while protecting contaminated soils.

Bradyrhizobium sp. sv. retamae nodulates Retama monosperma grown in a lead and zinc mine tailings in Eastern Morocco.

The aim of this work was to characterize and identify some bacteria isolated from the root nodules of Retama monosperma grown in Sidi Boubker lead and zinc mine tailings. Very few root nodules were obtained on the root nodules of R. monosperma grown in these soils. The three bacteria isolated from the root nodules were tolerant in vitro to different concentrations of heavy metals, including lead and zinc. The rep-PCR experiments showed that the three isolates have different molecular fingerprints and were considered as three different strains. The analysis of their 16S rRNA gene sequences proved their affiliation to the genus Bradyrhizobium. The analysis and phylogeny of the housekeeping genes atpD, glnII, gyrB, recA, and rpoB confirmed that the closest species was B. valentinum with similarity percentages of 95.61 to 95.82%. The three isolates recovered from the root nodules were slow-growing rhizobia capable to renodulate their original host plant in the presence of Pb-acetate. They were able to nodulate R. sphaerocarpa and Lupinus luteus also but not Glycine max or Phaseolus vulgaris. The phylogeny of the nodA and nodC nodulation genes as well as the nifH gene of the three strains showed that they belong to the symbiovar retamae of the genus Bradyrhizobium. The three strains isolated could be considered for use as inoculum for Retama plants before use in phytoremediation experiments.

Metal accumulation, growth and nutrition of Vernonia polyanthes exposed to lead nitrate and arbuscular mycorrhizal fungi

The association between plants and arbuscular mycorrhizal fungi (AMF) can be used to bioremediate areas contaminated by metals. The objectives of this work were to evaluate the lead (Pb2+) phytoaccumulation capacity, morpho-physiology and nutrition responses of Vernonia polyanthes exposed to a solution amended with concentrations of lead nitrate and arbuscular mycorrhizal fungi. The treatments consisted of increasing doses of Pb2+ as lead nitrate [Pb(NO3)2], two strains of AMF and an absolute control without lead and AMF. Lead negatively affected some morphophysiological variables, reduced 27.3, 25.63, 30.60, and 56.60% shoot length, root collar diameter, number of leaves and leaf area, respectively, besides reducing decreasing chlorophyll a. Lead accumulated in the shoot and roots, the latter at the highest concentrations. However, the translocation factor was above 1, indicating low efficiency. The bioaccumulation factor referring to the roots were above 1. The fungi colonization rate was low, 3.31% for Gigaspora margarita and 2.33% for Acaulospora morrowiae. However, the absorption of lead increased, reflecting in lower values of chlorophyll a, dry mass of root and diameter. Results indicated that the arboreal species V. polyanthes tolerate high concentrations of lead and can accumulate significant amounts in the roots. AMF increase the accumulation of lead in the shoot and can be used in projects aimed at the phytoextraction of metals.

Interactive effect of compost application and inoculation with the fungus Claroideoglomus claroideum in Oenothera picensis plants growing in mine tailings.

Different techniques have been developed for the remediation of Cu contaminated soils, being the phytoremediation a sustainable and environmentally friendly strategy, but its use in mine tailings is scarce. Arbuscular mycorrhizal fungi (AMF) can decrease the Cu concentration in plants by favouring the stabilization of this metal through different mechanisms such as the production of glomalin, immobilization in the fungal wall of hyphae and spores, and the storage of Cu in vacuoles. Additionally, the use of organic amendments promotes the beneficial effects produced by AMF and improves plant growth. Based on the above, the aim of this study was to determine the effect of AMF inoculation and compost application at different doses on the growth of Oenothera picensis in a Cu mine tailing. One group of plants were inoculated with Claroideoglomus claroideum (CC) and other was non-inoculated (NM). Both CC and NM were grown for two month under greenhouse conditions in pots with the Cu mine tailing, which also had increasing compost doses (0%, 2.5%, 5%, and 10%). Results showed greater biomass production of O. picensis by CC up to 2-fold compared with NM. This effect was improved by the compost addition, especially at doses of 5% and 10%. Therefore, the increase of mycorrhizal and nutritional parameters in O. picensis, and the decreasing of Cu availability in the mine tailing, promoted the production of photosynthetic pigments together with the plant growth, which is of importance to accomplish phytoremediation programs in Cu mine tailings.

Barriers for plant establishment in the abandoned tailings of Nacozari, Sonora, Mexico: the influence of compost addition on seedling performance and tailing properties.

Past mining activities have left a legacy of abandoned mine tailing deposits whose metal contaminants poses serious risks to ecosystems and human health. While the development of a vegetated cover in mine tailings can help in mitigating these risks, the local factors limiting plant establishment in these sites are not well understood, restricting phytostabilization efforts. Here, we explore some of the barriers that limit seedling establishment of two species (Vachellia farnesiana and Prosopis velutina) in a mine tailing deposit located in Nacozari, Sonora, Mexico, and assess whether compost addition can help in overcoming these barriers in pot and field experiments. Our field observations found 20 times more carbon and at least 4 times more nitrogen concentration in areas under vegetated patches than in non-vegetated areas, while a previous study found no difference in metal concentrations and other physicochemical parameters. This suggests that organic matter and nutrients are a major limitation for plant establishment. In agreement with this, species failed to establish without compost addition in the field experiment. Compost addition also had a positive effect on biomass accumulation, pH and microbial activity, but increased the substrate soluble concentration of As, Cu, and Zn. Nonetheless, only Cu, K, and Mo in P. velutina accumulated in tissues at levels considered toxic for animal consumption. Our study documents that compost addition facilitated plant establishment for the phytostabilization of mine tailings and help to prevent the dispersion of most metal contaminants via animal consumption. We encourage the use of complementary strategies to minimize the risk of dispersion of metal contaminants.

Phytostabilization of Pb and Cd polluted soils using Helianthus petiolaris as pioneer aromatic plant species.

The area of soils polluted with heavy metals is increasing due to industrialization and globalization. Aromatic plant species can be a suitable alternative way for agricultural valorization and phytomanagement of such soils by the commercialization of essential oils avoiding risks for the food chain. The potential of growing Helianthus petiolaris in heavy metal polluted soils was assessed in pot experiments using spiked soils and soils from a shooting range. In terms of phytostabilization, H. petiolaris could grow in soils containing 1000 mg/kg Pb2+, 50 mg/kg Cd2+, accumulating more than three times the soil Cd content in the aerial parts and translocating significant amounts of Pb to the aerial parts when growing in soils polluted with up to 500 mg/kg Pb. When phytostabilization is considered, phytotoxicity of heavy metals strongly depends on the rhizospheric microbial communities, either by mitigating trace element phytotoxicity or promoting plant growth via phytohormone production. So, the effects of heavy metals on the diversity of the rhizospheric bacterial community were assessed using DNA-fingerprinting.