Hatchery tanks induce intense reduction in microbiota diversity associated with gills and guts of two endemic species of the São Francisco River.

The São Francisco River (SFR), one of the main Brazilian rivers, has suffered cumulative anthropogenic impacts, leading to ever-decreasing fish stocks and environmental, economic, and social consequences. Rhinelepis aspera and Prochilodus argenteus are medium-sized, bottom-feeding, and rheophilic fishes from the SFR that suffer from these actions. Both species are targeted for spawning and restocking operations due to their relevance in artisanal fisheries, commercial activities, and conservation concerns. Using high-throughput sequencing of the 16S rRNA gene, we characterized the microbiome present in the gills and guts of these species recruited from an impacted SFR region and hatchery tanks (HT). Our results showed that bacterial diversity from the gill and gut at the genera level in both fish species from HT is 87% smaller than in species from the SFR. Furthermore, only 15 and 29% of bacterial genera are shared between gills and guts in R. aspera and P. argenteus from SFR, respectively, showing an intimate relationship between functional differences in organs. In both species from SFR, pathogenic, xenobiont-degrading, and cyanotoxin-producer bacterial genera were found, indicating the critical pollution scenario in which the river finds itself. This study allowed us to conclude that the conditions imposed on fish in the HT act as important modulators of microbial diversity in the analyzed tissues. It also raises questions regarding the effects of these conditions on hatchery spawn fish and their suitability for restocking activities, aggravated by the narrow genetic diversity associated with such freshwater systems.

The life cycle of Philophthalmus aylacostoma n. sp. (Trematoda: Philophthalmidae), a new eye fluke species transmitted by Aylacostoma spp. (Gastropoda: Thiaridae) in Brazil.

Philophthalmus is a cosmopolitan genus of digeneans that includes ocular parasites of birds and mammals. Despite broad distribution and veterinary importance of these digeneans, there are still gaps in knowledge about their diversity and biology, especially in South America. Herein, we conducted morphological, life cycle, and molecular studies of megalurous cercariae found in aquatic gastropod molluscs Aylacostoma chloroticum and A. tuberculatum collected in the São Francisco River, Brazil. Adult parasites reared experimentally in the eyes of chicks are described here as Philophthalmus aylacostoma n. sp. The new species differs from its congeners known in the Americas by a combination of traits, including the sucker width ratio, the oral sucker to pharynx width ratio, egg size, and the type of vitellarium in adult forms. The new species is morphologically closest to Philophthalmus megalurus, from which it differs by the smaller body and larger eggs, as well as by the measurements of cercariae and the family of snails that act as the intermediate host. Molecular phylogenetic analysis based on 28S rDNA and comparison of cox1 sequences confirm that P. aylacostoma n. sp. is distinct from four previously sequenced named species of the genus. Moreover, cox1 sequences revealed conspecificity of our specimens with an isolate of Philophthalmus sp. previously reported, also in thiarid snails, in Paraná River, Brazil. The interspecific divergence in cox1 between the new species and other species with sequences available for comparison varied between 12 and 15%.

Stepwise aggregation method for the WQI-CCME to generate a water quality profile for a long river: case study-São Francisco River, Brazil.

This paper presents a new aggregation method to calculate the WQI-CCME, named stepwise aggregation method, to communicate the water quality of the São Francisco River, Brazil, a river of national expression, with more than 2700 km long. Differently from the original proposal for the WQI-CCME, the new aggregation method uses data from a single monitoring campaign, to avoid the loss of data, as many variables are not systematically measured in all monitoring campaigns. The stepwise aggregation method also requires to combine data from a minimum of four monitoring locations per section of the river, to attend the statistical requirement, a proposal already presented in the literature. After using the first four points, the next calculation requires to abandon the first point (upstream) and to include the next point (downstream), and the sequential calculation will generate the results to build the river water quality profile. In the present work, with 31 monitoring points, the stepwise aggregation method allowed to calculate the WQI-CCME for 25 sections. The stepwise method has the advantage of multiplying the number of index calculations, allowing to construct a more uniform water quality profile for a long river. The choice of using the WQI-CCME in this work, is because this index has many successful applications all over the world, favored by its flexibility of parameter selection, ease of calculation, adaptability to legal requirements of different locations and different water uses. The WQI-CCME profiles were compared with those for the WQI-CETESB, an index derived from the WQI-NSF and extensively used by the Brazilian Monitoring Programs. The comparison demonstrated similarity, only if the same number of parameters were used, but WQI-CETESB gives better water quality, similarly reported for the WQI-NSF, which systematically classify the water quality in the superior range. In this paper, the calculation of the WQI-CCME, with 38 and 20 parameters, confirmed the dependence of the WQI-CCME factors (F1, F2) on the number of variables considered. The calculation with more variables (38) gave better results for the water quality than the calculation with less variable (20). Thus, while the application of the WQI-CCME has a variety of advantages, it requires careful evaluation about the variables to use, and the objectives for the index calculation, as considered by many authors.

Seasonal geochemical trends and pollution assessment of bottom sediments in the São Francisco hydrographic basin, Brazil: the Três Marias Reservoir.

The Três Marias Reservoir is the ninth largest reservoir in Brazil, becoming crucial for national strategic development. However, many anthropic activities may affect the sediment quality, promoting the need for a proper environmental assessment. This research appraised the seasonal influences on the Três Marias Reservoir's sediment geochemistry, elucidating possible anthropogenic impacts. The concentrations of Mg, Al, Ca, Cr, Fe, Co, Cu, Cd, Ti, Mn, Ni, Zn, Ba, and Pb were measured in 78 samples of bottom sediments regarding the two seasons of the area, a dry winter and rainy summer. The median ± 2 median absolute deviation (MAD) settled the geochemical background and environmental thresholds for the two seasons. The sediment quality guidelines CONAMA 344/12 highlight the possible adverse ecological effects of pollutants. The hierarchical clustering analysis, the geoaccumulation index, and the pollution load index delineated the polluted zones. The pollution load index ranges from 0.25 to 2.28 in the dry season and 0.56 to 2.11 in the rainy season, defining three affected zones in the reservoir. Forestry and agriculture are the probable pollution sources, reaching warning levels that should be considered in further environmental strategies.

Genetic evaluation of migratory fish: Implications for conservation and stocking programs.

Fish stocking programs have been implemented to mitigate the blockage of original riverbeds by the construction of hydropower dams, which affects the natural migration of fish populations. However, this method raises concerns regarding the genetic rescue of the original populations of migratory fish species. We investigated the spatial distribution of genetic properties, such as genetic diversity, population structure, and gene flow (migration), of the Neotropical migratory fish Prochilodus costatus in the Três Marias dam in the São Francisco River basin, Brazil, and examined the possible effects of fish stocking programs on P. costatus populations in this region. In total, 1,017 specimens were sampled from 12 natural sites and a fish stocking program, and genotyped for high-throughput sequencing at 8 microsatellite loci. The populations presented low genetic variability, with evidence of inbreeding and the presence of only four genetic pools; three pools were observed throughout the study region, and the fourth was exclusive to one area in the Paraopeba River. Additionally, we identified high unidirectional gene flow between regions, and a preferred migratory route between the Pará River and the upper portion of the São Francisco River. The fish stocking program succeeded in transposing the genetic pools from downstream to upstream of the Três Marias dam, but, regrettably, promoted genetic homogenization in the upper São Francisco River basin. Moreover, the data show the fragility of this species at the genetic level. This monitoring strategy could be a model for the development of conservation and management measures for migratory fish populations that are consumed by humans.

Increasing the known biodiversity of cnidarian parasites of bryconid fishes from South America: two novel Myxobolus species with ultrastructure and ssrDNA-based phylogeny.

This study increases the known biodiversity of cnidarian parasites in neotropical bryconid fishes. Two novel Myxobolus species are described based on morphology, ultrastructure and small subunit ribosomal DNA (ssrDNA) sequencing: Myxobolus vetuschicanus n. sp. infecting fins of Salminus franciscanus and Myxobolus mineirus n. sp. infecting the mesentery of Brycon orthotaenia from the São Francisco River basin, Minas Gerais State, Brazil. Ultrastructural analysis of the two species revealed an asynchronous sporogenesis process, with germinative cells and young developmental stages of myxospores in the periphery of the plasmodia. In M. vetuschicanus n. sp., the plasmodia were surrounded by a layer of fibroblasts and in M. mineirus n. sp., the plasmodial membrane had direct contact with the host tissue. The phylogenetic analysis based on the ssrDNA of Henneguya/Myxobolus species showed that the two novel Myxobolus species grouped in subclades together with other parasite species of bryconid fishes.

Taxonomy, phylogeny and host-parasite interaction of two novel Myxobolus species infecting Brycon orthotaenia from the São Francisco River, Brazil.

Two new Myxobolus species were described infecting Brycon orthotaenia from the São Francisco River, in the state of Minas Gerais, Brazil. From a total of 39 B. orthotaenia collected, two specimens (5.1%) exhibited infection of the ovary and 12 specimens (30.8%) displayed infection of the liver. The plasmodia of both Myxobolus species were white and spherical measuring around 1 mm in length. The plasmodium found in the ovary showed mature myxospores, which were oval shaped from the frontal view and measured 9.2-11.0 (9.8 ± 0.4) µm in length, 5.9-6.9 (6.5 ± 0.3) µm in width and 4.6-5 (4.9 ± 0.1) µm in diameter. The two polar capsules were the same size and measured 3.9-6.2 (4.7 ± 0.5) µm in length and 1.8-2.4 (2.1 ± 0.2) µm in width. The polar tubules had 9 coils. The plasmodium found in the liver showed mature myxospores which were ellipsoidal in shape from the frontal view and measured 10.0-11.4 (10.7 ± 0.5) µm in length, 7.3-8.6 (8.1 ± 0.4) µm in width and 5.3-7.0 (6.8 ± 0.4) µm in diameter. The two polar capsules were the same size and measured 4.2-5.4 (4.9 ± 0.3) µm in length and 1.9-2.9 (2.7 ± 0.3) µm in width. The polar tubules had 8 coils. Ultrastructural analysis revealed an asynchronous sporogenesis process, with young developmental myxospore stages more often found in the periphery of the plasmodium and mature myxospores in the centre of the plasmodium. The plasmodial wall was formed by a single membrane which was not surrounded by a layer of host tissue. A thick layer of fibrous material was found in the peripheral ectoplasm close to the plasmodial wall of the plasmodium found in the ovary. Phylogenetic analysis based on the small-subunit ribosomal DNA - ssrDNA sequences and using the closest myxozoan sequences to each one of the species studied here based on previous GenBank data and Henneguya/Myxobolus/Thelohanellus species parasitizing fish from South American, revealed that the new species are grouped in a subclade together with other Myxobolus species parasitizing bryconid hosts.

Using GRACE to quantify the depletion of terrestrial water storage in Northeastern Brazil: The Urucuia Aquifer System.

Covering a plateau area of approximately 125,000 km2, the Urucuia Aquifer System (UAS) represents a national strategic water resource in the drought-stricken Northeastern part of Brazil. Variations in terrestrial water storage (TWS) extracted using a three-model-ensemble from the Gravity Recovery and Climate Experiment (GRACE) mission showed a negative balance equal to water stress. Monthly GRACE-derived water storage changes from 2002 to 2014 were compared with those derived from an independent hydrologic water balance of the region using in situ measurements and estimated evapotranspiration rates. Trend analyses revealed a TWS depletion rate of 6.5 ±â€¯2.6 mm yr-1, but no significant decline in precipitation as observed from available data records. Water storage depletion was found to be driven by anthropogenic impacts rather than by natural climatic variability. The obtained results demonstrate that GRACE is able to adequately capture water storage changes at the subregional scale, particularly during dry seasons.

Integrative systematics unveils the controversial identity of Engraulidae fishing stocks in a Neotropical estuary, northeast Brazil

In Brazil, the use and diversity of the common names of fish species, coupled with taxonomic uncertainties, hinder the reliability of fishing statistical data. In this scenario, there are the so-called pilombetas of the São Francisco River, an important fishing resource in region. Despite its importance, the real diversity of species identified in the area remains obscure. In order to properly identify and delimit the species popularly known as pilombetas, an integrative approach involving traditional taxonomy, geometric morphometrics and molecular systematics was applied. Results from geometric morphometrics and molecular analyses were consistent with the results of the traditional morphological analysis, also indicating the delimitation of six taxa belonging to Engraulidae in the lower São Francisco River. In addition, species delimitation methods revealed an intrapopulation genetic divergence of 1.7% for Lycengraulis grossidens. The results revealed that the currently known richness species of Engraulidae in the studied area has been underestimated. Thus, an updated taxonomic key is herein proposed for the Engraulidae species from the lower São Francisco River and estuary. The integrative analysis approach revealed to be effective to address taxonomic questions and help the management of stocks, ensuring the maintenance of local diversity of fishes in the Neotropical region.(AU)

No Brasil, o uso e a diversidade dos nomes comuns para espécies de peixes, associado a incertezas taxonômicas, dificultam a confiabilidade dos dados estatísticos da pesca. Nesse cenário, existem as chamadas pilombetas do rio São Francisco, um importante recurso pesqueiro da região. Apesar de sua importância, a real diversidade de espécies identificadas na área permanece obscura. Para identificar adequadamente as espécies conhecidas como pilombetas, uma abordagem integrativa envolvendo taxonomia tradicional, morfometria geométrica e sistemática molecular foi aplicada. Os resultados das análises moleculares e de morfometria geométrica foram consistentes com os resultados da análise morfológica tradicional, indicando também a delimitação de seis táxons pertencentes a Engraulidae no baixo São Francisco. Além disso, os métodos de delimitação de espécies revelaram divergência genética intrapopulacional de 1,7% em Lycengraulis grossidens. Nossos dados revelaram que a riqueza de espécies atualmente conhecida de Engraulidae na área estudada é subestimada. Assim, uma chave taxonômica atualizada é aqui proposta para as espécies de Engraulidae do baixo rio São Francisco e seu estuário. A abordagem de análise integrativa revelou ser efetiva para tratar de questões taxonômicas e ajudar no gerenciamento de estoques, garantindo a manutenção da diversidade local de peixes na região Neotropical.(AU)

Freshwater fishes of the Bahia State, Northeastern Brazil / Peixes de água doce do Estado da Bahia, Nordeste do Brasil

Abstract: This work was carried out from the assessment of the conservation status of the freshwater ichthyofauna from Bahia State. The inventory data and species distribution were obtained from the specialized scientific literature and representative ichthyological collections. A total of 281 native species was recorded in Bahia State, distributed in the Northeastern Mata Atlantica (NMA) and São Francisco (SFR) freshwater ecoregions. There was a larger number of species in the NMA (187 spp.), composed by several coastal basins, than in the SFR (134 spp.), composed by São Francisco river basin. Among the 30 families recorded, Characidae and Rivulidae were the most representative, with 53 and 48 species, respectively. The conservation status of 214 species was assessed and 33 of them (15%) were included in the IUCN threat categories. Of these, 11 species were classified as vulnerable (VU), 12 as endangered (EN), and 10 as critically endangered (CR). Most threatened species (n = 14) belongs to the family Rivulidae. The larger number of threatened species in the NMA: (n = 23) is mainly related to the high endemism of restricted-range species associated with the human occupation impacts along the coastal regions. In the SFR, most of threatened species are annual killifishes, which are locally disappearing due to increasing degradation of their temporary habitats.

Resumo: Este trabalho foi realizado a partir da avaliação do estado de conservação da ictiofauna de água doce do Estado da Bahia. Os dados de inventário e distribuição das espécies foram obtidos a partir da literatura científica especializada e de coleções ictiológicas representativas. Um total de 281 espécies nativas foi registrado no Estado da Bahia, distribuídas nas ecorregiões de água doce Mata Atlântica Nordeste (NMA) e São Francisco (SFR). A riqueza de espécies foi maior na NMA (187 spp.), composta por diversas bacias costeiras, do que na SFR (134 spp.), composta pela bacia do rio São Francisco. Das 30 famílias registradas, as mais representativas foram Characidae e Rivulidae, com 53 e 48 espécies, respectivamente. O estado de conservação de 214 espécies foi avaliado e 33 (15%) destas foram classificadas em alguma categoria de ameaça da IUCN. Destas, 11 foram classificadas como vulneráveis (VU), 12 em perigo (EN) e 10 criticamente em perigo (CR). A maioria das espécies ameaçadas (n = 14) pertence à família Rivulidae. O elevado número de espécies ameaçadas na NMA: (n = 23) está associado ao alto endemismo das espécies com distribuição restrita e aos impactos da ocupação humana ao longo da região costeira. Na SFR, a maioria das espécies ameaçadas é de peixes anuais, que estão localmente desaparecendo devido à degradação crescente de seus habitats temporários.

Saúde dos rios, saúde dos povos e do planeta: um olhar sobre o documentário Saúde! Velho Chico, de Stella Oswaldo Cruz Penido e Eduardo Vilela Thielen / Health of the rivers, health of the peoples and of the Earth: an analysis of the documentary Saúde! Velho Chico, by Stella Oswaldo Cruz Penido e Eduardo Vilela Thielen / Salud de los ríos, salud de los pueblos y de lo planeta: una análisis del documental Saúde! Velho Chico, de Stella Oswaldo Cruz Penido y Eduardo Vilela Thielen

Análise do documentário Saúde! Velho Chico, de Stella Oswaldo Cruz Penido e Eduardo Vilela Thielen, tomando-o como registro, a um só tempo, dos padrões históricos de interação com o Rio São Francisco e de alternativas capazes de construir uma relação mais sinérgica e sustentável com as águas fluviais. O filme contrapõe as metanarrativas do desenvolvimento ­ legitimadoras de intervenções como hidrelétricas, barragens, projetos de irrigação para a agricultura industrial e o controverso projeto de sua transposição ­ a formas de relação estabelecidas pelas diversas populações ribeirinhas, das quais a vida, o corpo e as culturas são construídos em estreita simbiose com o rio.

This review analyzes the documentary Saúde! Velho Chico, scripted and directed by Stella Oswaldo Cruz Penido and Eduardo Vilela Thielen, taking it as a record of the historical patterns of interaction with the Rio São Francisco (São Francisco River) and at the same time of alternatives based on the building of more synergistic and sustainable relationships with river waters. In this sense, the film contrasts the metanarratives of development ­ which legitimize interventions such as hydroelectric dams, irrigation projects for industrial agriculture as well as the controversial transfer of the São Francisco River project ­ with relationships established by the riverside populations, who have a close symbiosis between their life, body and cultures and the river.

Análisis del documental Saúde! Velho Chico, con guión y dirección de Stella Oswaldo Cruz Penido y Eduardo Vilela Thielen, entendido como un registro, al mismo tiempo, de los patrones históricos de interacción con el Rio São Francisco (río San Francisco) y de alternativas capaces de construir una relación más sinérgica y sostenible con las aguas de los ríos. La película contrasta las metanarrativas del desarrollo ­ que legitiman intervenciones como represas hidroeléctricas, proyectos de riego para la agricultura industrial y el controvertido proyecto de transposición del río ­ con formas de relación establecidas por las diversas poblaciones ribereñas, que tienen la vida, el cuerpo y las culturas en estrecha simbiosis con el río.

A dune with a view: the eyes of a neotropical fossorial lizard.

BACKGROUND: Lizards are excellent models to study the adaptations of the visual system to different scenarios, and surface-dwelling representatives have been relatively well studied. In contrast, very little is known about the functional anatomy of the eyes of fossorial lineages, and properties such as the light transmission by the ocular media have never been characterised in any fossorial species. Some lizards in the family Gymnophthalmidae endemic to the sand dunes of North Eastern Brazil have evolved sand-burrowing habits and nocturnal activity. Lizards in the sister group to Gymnophthalmidae, the family Teiidae, have decidedly diurnal and epigeal lifestyles, yet they are equally poorly known in terms of visual systems. We focussed on the eye anatomy, photoreceptor morphology and light transmittance properties of the ocular media and oil droplets in the gymnophthalmid Calyptommatus nicterus and the teiid Ameivula ocellifera. RESULTS: The general organisation of the eyes of the fossorial nocturnal C. nicterus and the epigeal diurnal A. ocellifera is remarkably similar. The lenses are highly transmissive to light well into the ultraviolet part of the spectrum. The photoreceptors have the typical cone morphology, with narrow short outer segments and oil droplets. The main difference between the two species is that C. nicterus has only colourless oil droplets, whereas A. ocellifera has colourless as well as green-yellow and pale-orange droplets. CONCLUSIONS: Our results challenge the assumption that fossorial lizards undergo loss of visual function, a claim that is usually guided by the reduced size and external morphology of their eyes. In the case of C. nicterus, the visual system is well suited for vision in bright light and shows specialisations that improve sensitivity in dim light, suggesting that they might perform some visually-guided behaviour above the surface at the beginning or the end of their daily activity period, when light levels are relatively high in their open dunes habitat. This work highlights how studies on the functional anatomy of sensory systems can provide insights into the habits of secretive species.

Upstream and downstream migration speed of Prochilodus costatus (Characiformes: Prochilodontidae) in upper São Francisco basin, Brazil

Details of migration dynamics of Neotropical fishes are poorly understood. This study aimed to examine upstream (spawning) and downstream (post-spawning) migration speeds, of curimatá-pioa (Prochilodus costatus) in the São Francisco River basin, southeast Brazil. Most upstream movements were recorded in October and November, in two well-defined migration windows, and downstream movements were frequent from December to March. Fish migrated upstream at an average migration speed of 34.4 km day-1 and no significant differences were detected in their speed between sexes and migration window they selected to migrate. No relationship was detected between upstream migration speed and biometric measures of tagged individuals. Upstream migrations speeds were significantly higher for fish that swam longer prior to reach telemetry stations in the same season, indicating that swimming performance may take some time to achieve its peak in upstream migration. Fish migrated downstream at an average migration speed of 97.7 km day-1, what is close to passive swimming in São Francisco River, and no significant differences in speed were detected between sexes or capture sites. The migration speeds measured here are the highest ever recorded for the genus Prochilodus and are among the highest reported for Neotropical migratory fish.(AU)

Detalhes acerca da dinâmica migratória de peixes neotropicais são pouco conhecidos. O objetivo deste estudo foi analisar as velocidades em migração ascendente (reprodutiva) e descendente (pós-reprodutiva) da curimatá-pioa (Prochilodus costatus) no rio São Francisco, Brasil. A migração ascendente concentrou-se nos meses de outubro e novembro, em duas janelas migratórias, e a descendente de dezembro a março. Os peixes alcançaram velocidade migratória média de 34,4 km dia-1 em migração ascendente não sendo observadas diferenças nas velocidades em relação ao sexo e janela migratória em que o peixe migrou. Não foi observada relação entre velocidade migratória e características biométricas dos peixes. Diferenças nas velocidades migratórias ascendentes foram observadas para peixes que nadaram maiores distâncias antes de passar pelas estações de telemetria, indicando que o pico de desempenho natatório é alcançado algum tempo depois de iniciado o movimento ascendente. Em migração descendente a média de velocidade migratória foi de 97,7 km dia-1, próxima à natação passiva, e não foram observadas diferenças entre as velocidades em relação a sexo e ponto de marcação dos peixes. As velocidades migratórias medidas neste estudo são as maiores já registradas para o gênero Prochilodus e estão entre as maiores registradas para peixes migradores neotropicais.(AU)

Proposição de melhorias para o Sistema de Cobrança pelo Uso da Água Bruta da Bacia Hidrográfica do Rio São Francisco / Proposal for improvements in the Raw Water Charge System of the São Francisco River Basin - Brazil

RESUMO A cobrança pelo uso da água bruta no Brasil foi introduzida pela Lei nº 9.433, de 08 de janeiro de 1997, como um instrumento econômico de gestão dos recursos hídricos brasileiros, com o intuito de induzir os agentes usuários ao uso racional da água, reconhecer o valor econômico da água e obter recursos financeiros. A Bacia Hidrográfica do Rio São Francisco implementou o seu sistema de cobrança pelo uso da água em julho de 2010, não passando por nenhuma modificação ou melhoria desde então. Nesta pesquisa, apresenta-se uma análise desse sistema de cobrança, identificando os aspectos passíveis de aperfeiçoamento e a simulando as modificações propostas para a Região Hidrográfica do Submédio São Francisco. Os resultados indicaram que é necessário rever os valores de cobrança adotados atualmente. Os Preços Públicos Unitários (PPUs) apresentam defasagem acumulada em torno de 50%, mediante as taxas inflacionárias brasileiras. Os aspectos quantitativo, qualitativo e de proteção a situações emergenciais, atualmente existentes na metodologia de cobrança, não se mostram suficientes para auxiliar na sustentabilidade hídrica da bacia. Entre as alterações sugeridas para o sistema de cobrança estão a consideração de situações de escassez hídrica, de prioridade no uso, de tratamento dos efluentes e de tecnologia de irrigação. Tais alterações revelam-se eficazes ao reduzir/aumentar consideravelmente os valores a serem pagos pelos usuários, podendo, assim, induzir ações preventivas e de redução dos desperdícios.

ABSTRACT A raw water charge in Brazil was introduced by Law nº 9.433 on 8 January, 1997, as an economic instrument to manage Brazil's water resources. Its purpose is to induce water users to rationally consume water, to recognize the economic value of water, and to obtain financial resources. The São Francisco River Basin implemented a raw water charge in July of 2010, and nothing has changed or improved since. This paper presents an analysis of this raw water charge system, identifies aspects that can be improved, and simulates the proposed improvements for water users of the Submédio São Francisco Hydrographic Region. The results showed that it is necessary to review the values currently used in the raw water charge system. The public unit prices (PUP) have an accumulated lag of around 50%, due to Brazilian inflation rates. The measurement of quantitative and qualitative aspects in addition to the protection against emergency situations that currently exist in the raw water charge system are not sufficient to assist in the basin's water sustainability. Changes suggested for the raw water charge system include the consideration of water scarcity, priority in water use, the treatment of effluents and irrigation technology, among others. Such changes prove to be effective in considerably reducing or increasing amounts to be paid by users, and can thus induce preventive actions and reduce waste.

Representações da transposição do rio São Francisco na imprensa paraibana / Representations of transposition São Francisco River in the state of Paraíba press / Representaciones de la transposición del río San Francisco en la prensa de Pará

O presente estudo objetivou conhecer as representações sociais da transposição do rio São Francisco no Jornal da Paraíba entre 1999 e 2008. A coleta foi realizada no site do jornal, perfazendo um total de 1.346 reportagens. Para tratamento dos dados foi utilizado o software Alceste. Os resultados mostram discursos contra e a favor do projeto nos governos FHC e Lula. Os discursos contra o projeto dirigem-se ao estado degradado do rio São Francisco objetivado numa pessoa doente. Entidades civis e religiosas são os principais representantes desses discursos. Proferido por políticos, os discursos a favor do projeto ancoram-se no fenômeno da seca. A vazão do rio para saciar a sede e desenvolvimento econômico são elementos representacionais, que objetivados no nordestino retirante, defendem a transposição. Percebe-se então representações distintas nos dois governos, devendo ser entendidas juntamente aos grupos que as disseminam

This study aimed to identify the social representations of the São Francisco transposition in the Journal of Paraíba between 1999 and 2008. Data collection was conducted on the newspaper's website, in a total of 1346 reports. Data collected was analyzed by Alceste software. Results show speeches in favor and against the project during the FHC and Lula administrations. The speeches against the project are directed to the degraded state of the São Francisco River objectified in a sick person. Civil and religious entities are the main representatives of these speeches. Delivered by politicians, speeches in favor anchor in the drought phenomenon. The flow of the river to quench their thirst and economic development are representational elements, which objectified in the Northeast migrant, defend the transposition. Distinct representations in the two governments are then perceived, and it should be understood considering the groups which disseminates each representation

El presente estudio objetivó conocer las representaciones sociales de la transposición del río São Francisco en el diario Jornal da Paraíba entre 1999 y 2008. La recolección fue realizada en el sitio del periódico, totalizando un total de 1346 reportajes. Para el tratamiento de los datos se utilizó el software Alceste. Los resultados muestran discursos contra y a favor del proyecto en los gobiernos FHC y Lula. Los discursos contra el proyecto se dirigen al estado degradado del río San Francisco objetivado en una persona enferma. Entidades civiles y religiosas son los principales representantes de esos discursos Proferido por políticos, los discursos a favor del proyecto se apoyan en el fenómeno de la sequía. El caudal del río para saciar la sed y el desarrollo económico son elementos representacionales, que objetivados en el nordestino desplazado, defienden la transposición. Se percibe entonces representaciones distintas en los dos gobiernos, debiendo ser entendidas junto a los grupos que las diseminan

Fisheries, life-history and conservation status of the catfish pirá Conorhynchos conirostris (Ostariophysi: Siluriformes) in Brazil

Pirá, Conorhynchos conirostris (Valenciennes, 1840), a large migratory catfish endemic to the São Francisco River (SFR), is listed as threatened in the red lists of both Brazil and the state of Minas Gerais. Although fishing for pirá has been prohibited, it is still an important fishery resource, particularly in the middle SFR. We used historical and current occurrence and abundance data regarding pirá to determine if it meets the IUCN criteria of a threatened species. Pirá occurs in the main course of the SFR as well as in its major tributaries. Unlike the most well-known migratory fishes of the SFR, pirá does not use floodplain lakes as nurseries. In the first half of the 20th century, pirá occurred from the upper to the lower SFR. Currently, it is most abundant in the middle SFR, and is rare in the upper SFR and even rarer in the sub-middle SFR. Pirá has not been captured in the lower SFR since around the mid-1980's. Despite the reduction in its geographic distribution, we did not find evidence to justify considering pirá as threatened. Thus, we recommend that it be removed from the red lists of Brazil and the state of Minas Gerais.(AU)

Pirá, Conorhynchos conirostris (Valenciennes, 1840), um grande siluriforme migrador endêmico do rio São Francisco (RSF), está incluído como ameaçado nas listas vermelhas do Brasil e do estado de Minas Gerais. Embora sua pesca esteja proibida, ele ainda é recurso pesqueiro importante, particularmente no médio RSF. Utilizamos dados históricos e atuais de ocorrência e abundância do pirá para determinar se ele atende aos critérios da UICN para ser classificado como ameaçado. Pirá ocorre na calha do RSF, bem como em seus principais afluentes. Ao contrário dos peixes migradores mais conhecidos do RSF, ele não usa lagoas das várzeas como berçários. Na primeira metade do século 20, pirá costumava ocorrer do alto ao baixo RSF. Atualmente, ele é mais abundante no médio RSF. A espécie é rara no alto RSF e parece ainda mais rara no sub-médio RSF. O pirá não é capturado no baixo RSF desde cerca da metade da década de 1980. Apesar da redução na distribuição geográfica, não encontramos evidências para classificar o pirá como espécie ameaçada e, portanto, recomendamos que ele seja removido das listas vermelhas do Brasil e do estado de Minas Gerais.(AU)

Evaluation of violations in water quality standards in the monitoring network of São Francisco River basin, the third largest in Brazil.

The São Francisco River is the largest river located entirely within Brazil, and water scarcity problems have been a major concern of Brazilian society and government. Water quality issues are also a concern and have worsened with the recent intensification of urbanization and industrialization. In this study, violations to water quality standards established by local legislation were calculated as a percentage for 26 selected parameters over a monitoring period of 14 years. The violation percentages were analyzed spatially using the Kruskal-Wallis test, followed by multiple comparison analysis. Temporal analysis was performed using the Mann-Kendall test and Spearman correlation. Some parameters could be identified as cause for concern due to high violation levels, such as the fecal coliform indicator (FCI) and phosphorus-both related to domestic and effluent disposal without treatment or with insufficient treatment-and color, turbidity, manganese, and total suspended solids-which can be affected by erosive processes of natural and anthropogenic causes. The study found that these violations are concentrated in the most urbanized and industrialized areas of the basin. Some metallic parameters, such as iron and arsenic violations, may be related to mining activities in the rich soil of the Iron Quadrangle area located within the Minas Gerais State. Trend analysis results indicated that most monitoring stations did not have significant modification (elevation or reduction) trends over time, which, together with the high violation percentages, might indicate the maintenance of a scenario of constant pressure upon water resources, in particular in those more urbanized areas.

Detection of schistosomiasis in an area directly affected by the São Francisco River large-scale water transposition project in the Northeast of Brazil

Abstract INTRODUCTION: The development of the São Francisco River Integration Project [Projeto de Integração do Rio São Francisco (PISF)] in the State of Ceará, Brazil, has resulted in environmental and socioeconomic changes with potential risks to public health. We aimed to determine the presence of Schistosoma mansoni infections in schoolchildren (aged 7-14 years) and workers from the construction site in an area under the direct influence of the PISF in the municipality of Brejo Santo-CE, to aid in the prevention and control of schistosomiasis. METHODS: We conducted a cross-sectional study using two S. mansoni-detection methods: detection of S. mansoni eggs by the Kato-Katz parasitological method in stool samples (assessed in triplicate for each sample) and S. mansoni circulating cathodic antigen by the point-of-care immunochromatographic rapid test (POC-CCA) in urine. RESULTS In general, the positivity rates for S. mansoni detection were 1.9% (2/106) among schoolchildren and 2.9% (4/138) among workers. No child had evidence of S. mansoni eggs in their stools; 1.9% tested positive by the POC-CCA method. Among workers, two (1.4%) tested positive by the Kato-Katz test and three (2.2%) by the POC-CCA test. If the POC-CCA test results that were scored as traces were considered negative, then the positivity rates dropped to 0.9% and 0.7% for schoolchildren and workers, respectively. CONCLUSIONS: The active transmission of schistosomiasis in a region covered by the PISF was recognized, reinforcing the necessity to consolidate surveillance and control actions, as well as structural sanitation measures to reverse the social determinants of the disease.

Qualidade das águas superficiais do Médio São Francisco após a implantação dos perímetros irrigados de Gorutuba/Lagoa Grande e Jaíba / Surface water quality of the Middle San Francisco river basin after the implementation of irrigated perimeters of Gorutuba/Lagoa Grande and Jaíba

RESUMO Os perímetros irrigados de Gorutuba/Lagoa Grande e Jaíba localizam-se na porção mineira do Médio São Francisco, totalizando cerca de 31.493 ha. Essa região possui potencial de contaminar os recursos hídricos em decorrência da urbanização, do desmatamento e da utilização de fertilizantes e de agrotóxicos nas atividades agrícolas. O objetivo desta pesquisa foi investigar o impacto da irrigação na qualidade das águas superficiais da região, utilizando fontes de dados secundários de monitoramento. Foram selecionadas nove estações de monitoramento da qualidade da água superficial na região, que representavam diferentes usos e ocupação do solo. A análise de cluster e o teste não paramétrico de Kruskal-Wallis, seguido do teste de comparações múltiplas, foram utilizados para avaliar comparativamente as estações de amostragem, bem como identificar os parâmetros que apresentaram comportamentos diferenciados entre essas estações. Apesar de não ter sido possível observar um padrão de contaminação em função da localização das estações, detectou-se que as estações localizadas ao longo do Rio São Francisco não apresentaram diferenças significativas entre si, ainda que o uso e ocupação do solo fossem diferentes. A tendência temporal dos constituintes foi verificada através da aplicação do teste de hipótese de estacionariedade de Spearman, o qual detectou que a maioria dos dados analisados foram não estacionários, apresentando tendência de elevação ou redução ao longo do período analisado. Após a aplicação desses testes estatísticos, concluiu-se que, de maneira geral, a implantação de perímetros irrigados causou baixo impacto na qualidade das águas na região, no período estudado, considerando os parâmetros analisados neste trabalho.

ABSTRACT The irrigation perimeters of Jaíba and Gorutuba/Lagoa Grande are located in the Minas Gerais portion of the Middle São Francisco river basin, Brazil, occupying an area of approximately 31,493 ha. This region has the potential to contaminate water resources as a result of urbanization, deforestation and the application of fertilizers and pesticides in agricultural activities. The aim of this research was to investigate the impact of irrigation on surface water quality in the region, using secondary monitoring. Nine monitoring stations were selected in the region, which represent the diferente uses and occupation of land. The cluster analysis and the nonparametric Kruskal-Wallis test, followed by multiple comparisons, were used to comparatively evaluate the sampling stations and to identify which parameters showed different behavior among these stations. Although it was not possible to observe a pattern of contamination due to the location of the stations, it was found that the stations located along the São Francisco river showed no significant differences, although the use and occupation of land were different. The temporal trend of the constituents was verified by applying the test of the hypothesis of stationarity Spearmant, which found that most of the data analyzed were not stationary, with a tendency of increase or decrease over the analysis period. After the application of these statistical tests, it was concluded that, in general, the implementation of irrigated perimeters caused low impact in water quality in the region, in the period studied, considering the parameters analyzed in this study.

Águas da desunião nacional: representações da transposição do rio São Francisco / Waters of national disunity: representations of transposition São Francisco River

Nessa última década, as secas tornaram-se mais frequentes nas regiões brasileiras, por isso a água tem se tornado um bem constantemente debatido nos meios de comunicação. Entendendo a integração de rios com bacias hidrográficas como política pública fundamental de acesso à água, o presente estudo objetivou conhecer as representações sociais da transposição do rio São Francisco no Jornal Estado de Minas entre 1999 e 2008. A coleta foi realizada no site do jornal, perfazendo um total de 316 reportagens. Para tratamento dos dados foi utilizado o software Alceste. Os resultados mostram discursos contra e a favor do projeto nos governos FHC e Lula. Os discursos contrários ao projeto clamam pela revitalização do rio. Através das falas dos pescadores, o rio é objetivado na figura de uma pessoa doente em virtude do seu estado degradado e ancorado em épocas de matas ciliares preservadas. A revitalização seria a salvação do rio, tornando-se principal argumento contra o projeto. Paralelamente, o uso da transposição para fins econômicos torna-o questionável e ancorado ao histórico de obras públicas inconclusas no Nordeste e de objetivos escusos. Nos discursos favoráveis, propalados especialmente por políticos, a transposição é imprescindível para o desenvolvimento econômico do Nordeste. Tais desenvolvimentos são elementos representacionais ligados à ideia de seca, pois as estiagens contribuíram para as históricas migrações forçadas e estagnação do desenvolvimento da região. Dessa forma, na problemática da crise hídrica, a ideia da transposição como propulsor do desenvolvimento e remediação da seca, mostra-o como redentor do Nordeste/nordestino, explorado pelo jornal e propagandas governamentais. (AU)

In this last decade droughts have become more frequent in the Brazilian regions, where the water becomes constantly discussed in the media. Understanding the transposition of the São Francisco River as a fundamental public policy on these problems, this study aimed to understand the social representations of the transposition of the São Francisco River in the Estado de Minas newspaper from 1999 to 2008. The collection was made in the newspaper's website, making a total of 316 reports. The data collected was analyzed by Alceste software. The results show speeches against and favor of the project in the FHC and Lula administrations. Speeches against the project claim for the revitalization of the river. Through the speeches of fishermen, the river is objectified a sick person because of its degraded state and anchored in preserved riparian forests. The revitalization would be the salvation of the river, becoming main argument against the project. Same time, the use of transposition for economic purposes becomes questionable and anchored to the history of unfinished public constructions in the Northeast and shady goals. Speeches in favor, made especially by politicians, transposition is essential for the economic of the Northeast. Such development is representational element linked to the idea of drought, therefore, the aridity contributed to the historical migration and stagnation of the region. Thus, in the water crisis, the idea of transposition as a driver of development and remediation of drought, shows him as redeemer of the Northeast/northeastern, exploited by newspaper and government advertisements. (AU)