ABSTRACT
Mus musculus (Rodentia: Muridae) has generally been infected with a rodent hookworm Nippostrongylus brasiliensis. In this report, we present morphological and molecular identification of N. brasiliensis by light and scanning electron microscopy and PCR amplification of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the protein sequences encoded by cox1 gene, respectively. Despite the use of N. brasiliensis in many biochemistry studies from India, their taxonomic identification was not fully understood, especially at the species level, and no molecular data is available in GenBank from India. Sequence analysis of cox1 gene in this study revealed that the present specimen showed close identity with the same species available in GenBank, confirming that the species is N. brasiliensis. This study represents the first record of molecular identification of N. brasiliensis from India and the protein structure to better understand the comparative phylogenetic characteristics.
Subject(s)
Nippostrongylus/classification , Nippostrongylus/isolation & purification , Rodent Diseases/parasitology , Strongylida Infections/veterinary , Animal Structures/anatomy & histology , Animals , Cluster Analysis , DNA, Helminth/chemistry , DNA, Helminth/genetics , Electron Transport Complex IV/genetics , Female , India , Male , Mice , Microscopy , Nippostrongylus/anatomy & histology , Nippostrongylus/genetics , Phylogeny , Sequence Analysis, DNA , Strongylida Infections/parasitologyABSTRACT
Rhinosporiodiosis is a cosmopolitan disease of man and animals, endemic in India and Sri Lanka with main focus of infection in Southern Tamil Nadu. Uttar Pradesh (UP) is not known to be an endemic zone for this disease .We present here the first case of nasal Rhinosporiodiosis from this non-endemic zone.
ABSTRACT
Rhinosporiodiosis is a cosmopolitan disease of man and animals, endemic in India and Sri Lanka with main focus of infection in Southern Tamil Nadu. Uttar Pradesh (UP) is not known to be an endemic zone for this disease .We present here the first case of nasal Rhinosporiodiosis from this non-endemic zone.
ABSTRACT
Rhinosporiodiosis is a cosmopolitan disease of man and animals, endemic in India and Sri Lanka with main focus of infection in Southern Tamil Nadu. Uttar Pradesh (UP) is not known to be an endemic zone for this disease .We present here the first case of nasal Rhinosporiodiosis from this non-endemic zone.