ABSTRACT
Vascular malformations, which arise from anomalies in angiogenesis, encompass capillary, lymphatic, venous, arteriovenous and mixed malformations, each affecting specific vessel types. Historically, therapeutic options such as sclerotherapy and surgery have shown limited efficacy in complicated malformations. Most vascular malformations stem from hereditary or somatic mutations akin to oncogenic alterations, activating the PI3K-AKT-mTOR, RAS-MAPK-ERK, and G-protein coupled receptor pathways. Recognizing the parallels with oncogenic mutations, we emphasize the potential of targeted molecular inhibitors in the treatment of vascular malformations by repurposing anticancer drugs. This review delves into the recent development and future use of such agents for the management of slow- and fast-flow vascular malformations, including in more specific situations, such as prenatal treatment and the management of associated coagulopathies.
ABSTRACT
Vacuum-Assisted Sorbent Extraction (VASE) is a novel extraction technique that uses vacuum to facilitate the transfer of volatile compounds from the matrix to the sorbent. This technique was explored for extraction of volatiles from cape gooseberry fruit, for both qualitative and quantitative analyses. Selected extraction parameters were tested: sample size, extraction temperature and time, influence of tissue disintegration on release of volatiles, and also addition of Ag+1 ions in the form of AgNO3 to stop enzymatic formation of volatile compounds. For selected conditions (10 g sample, extraction for 30 min. at 40 °C of volatiles from blended fruit) quantitative aspects were explored. Twenty-two compounds of cape gooseberry were tested. The method was characterized with a very good linearity in a range of 10-5000 µg/kg and good reproducibility. The experiments proved the usefulness of VASE in both volatile profiling and quantitative analyses of cape gooseberry and in prospective other fruit.
Subject(s)
Fruit , Physalis , Volatile Organic Compounds , Volatile Organic Compounds/analysis , Volatile Organic Compounds/isolation & purification , Volatile Organic Compounds/chemistry , Physalis/chemistry , Fruit/chemistry , Vacuum , Reproducibility of Results , Plant Extracts/chemistry , Gas Chromatography-Mass Spectrometry/methods , Solid Phase Extraction/methodsABSTRACT
Cut flowers deteriorate rapidly after harvest, lasting mere days. To extend their vase life, various postharvest techniques are employed. Due to limited knowledge about the postharvest physiology of Alstroemeria cut flowers and the specific role of secondary compounds and antioxidant systems in their protection, this study investigated the optimal dosage of sodium nitroprusside (SNP) as a nitric oxide (NO) donor to enhance quality and antioxidant defenses. Preharvest foliar application of SNP at 0, 50, 100, and 200 µM followed by short-term pulsing treatments upon harvest at the same concentrations were applied in a factorial design. Results revealed that a preharvest 100 µM SNP treatment combined with a 50 µM postharvest pulse significantly increased the total amount of phenols (over 20%), antioxidant capacity (more than doubled), and the activity of two antioxidant enzymes (ascorbate peroxidase by over 35% and guaiacol peroxidase by about 20%). Notably, this combination also diminished ion leakage (by about 20%), ultimately extending the vase life by more than 40% compared to untreated plants. Therefore, SNP application at these specific dosages proves effective in bolstering Alstroemeria cut flower quality and vase life through enhanced total phenols and a strengthened antioxidant system.
Subject(s)
Antioxidants , Flowers , Nitroprusside , Nitroprusside/pharmacology , Flowers/drug effects , Flowers/physiology , Antioxidants/metabolism , Phenols/metabolism , Nitric Oxide Donors/pharmacology , Peroxidase/metabolism , Ascorbate Peroxidases/metabolismABSTRACT
Heterotrophic protists are vital in Earth's ecosystems, influencing carbon and nutrient cycles and occupying key positions in food webs as microbial predators. Fossils and molecular data suggest the emergence of predatory microeukaryotes and the transition to a eukaryote-rich marine environment by 800 million years ago (Ma). Neoproterozoic vase-shaped microfossils (VSMs) linked to Arcellinida testate amoebae represent the oldest evidence of heterotrophic microeukaryotes. This study explores the phylogenetic relationship and divergence times of modern Arcellinida and related taxa using a relaxed molecular clock approach. We estimate the origin of nodes leading to extant members of the Arcellinida Order to have happened during the latest Mesoproterozoic and Neoproterozoic (1054 to 661 Ma), while the divergence of extant infraorders postdates the Silurian. Our results demonstrate that at least one major heterotrophic eukaryote lineage originated during the Neoproterozoic. A putative radiation of eukaryotic groups (e.g., Arcellinida) during the early-Neoproterozoic sustained by favorable ecological and environmental conditions may have contributed to eukaryotic life endurance during the Cryogenian severe ice ages. Moreover, we infer that Arcellinida most likely already inhabited terrestrial habitats during the Neoproterozoic, coexisting with terrestrial Fungi and green algae, before land plant radiation. The most recent extant Arcellinida groups diverged during the Silurian Period, alongside other taxa within Fungi and flowering plants. These findings shed light on heterotrophic microeukaryotes' evolutionary history and ecological significance in Earth's ecosystems, using testate amoebae as a proxy.
Subject(s)
Ecosystem , Fossils , Heterotrophic Processes , Phylogeny , Biodiversity , Biological Evolution , Amoebozoa/genetics , Amoebozoa/classification , Amoeba/genetics , Amoeba/classification , Amoeba/physiology , Eukaryota/genetics , Eukaryota/classificationABSTRACT
For the first time, a method for the simultaneous analysis of fifteen polycyclic aromatic hydrocarbons (PAHs), including light and heavy PAHs, in açaí-based food products (AFPs) was developed using vacuum-assisted sorbent extraction (VASE) combined with gas chromatography-mass spectrometry (GC-MS). The method requires no organic solvents and is amenable to full automation. To achieve optimal analytical extraction conditions, VASE parameters including stirring rate, extraction time, desorption temperature, desorption time, preheat time, and preheat temperature were optimized using sequential multivariate optimization. The method was validated and yielded limits of quantification below 1 µg kg-1 for all analytes, with recoveries ranging from 65 % to 112 % and good precision (≤11 % relative standard deviation). Additionally, the greenness and practical aspects of the method were investigated using the Green Analytical Procedure Index (GAPI), eco-scale, and the Blue Applicability Grade Index (BAGI), respectively. The VASE-GC-MS approach is suitable for routine analysis and exhibits characteristics of a green analytical method. No PAHs were detected above the limits of detection in thirty samples of AFPs.
Subject(s)
Gas Chromatography-Mass Spectrometry , Limit of Detection , Polycyclic Aromatic Hydrocarbons , Gas Chromatography-Mass Spectrometry/methods , Polycyclic Aromatic Hydrocarbons/analysis , Polycyclic Aromatic Hydrocarbons/isolation & purification , Vacuum , Solid Phase Extraction/methods , Food Contamination/analysis , Green Chemistry Technology/methods , Food Analysis/methods , Reproducibility of ResultsABSTRACT
Repurposing anticancer drugs to vascular malformations has significantly improved patient outcomes. Complex Lymphatic Anomalies (CLA) are part of the spectrum of lymphatic malformations (LMs) that share similar oncogenic mutations to cancer. We report the case of a young patient with highly symptomatic CLA who was initially treated with sirolimus, due to the frequent involvement of the PI3K-AKT-mTOR pathway in CLA pathogenesis. Despite an initial reduction in symptoms, sirolimus progressively lost its effectiveness. After an unsuccessful attempt with trametinib alone, sirolimus was added to trametinib and resulted in a significant, rapid and sustained improvement in symptoms. This suggests that, contrary to current dogmas, combination therapy using sub-therapeutic doses targeting both the PI3K and RAS pathways retains efficacy without generating the toxicity known for combination therapies, and is beneficial in the management of CLAs and potentially other vascular anomalies.
Subject(s)
Lymphatic Abnormalities , Pyridones , Pyrimidinones , Sirolimus , Humans , Lymphatic Abnormalities/drug therapy , Lymphatic Abnormalities/pathology , Pyridones/therapeutic use , Pyrimidinones/therapeutic use , Sirolimus/therapeutic useABSTRACT
Specialty cut flowers are in demand, especially in the domestic market as they can be grown with low production costs without the need for specially equipped greenhouses and offer diversity in terms of form, texture, and colour. These products, which are widely cultivated in the USA, are not well known in Türkiye. One of the main problems of the Turkish cut flower sector is its dependence on foreign inputs and the lack of product diversity. Therefore, specialty cut flower production can be an alternative crop for Türkiye, which has climatic advantages. The cut flower potential of plants such as Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, Centaurea cyanus, and Cleome spinosa that are commonly grown in gardens has been evaluated. After harvesting these flowers grown in an open field in June-October 2020, the stem length (cm), stem thickness (mm), flower length (cm), flower diameter (cm), flower weight (g), and vase life (days) were measured. As a result of the evaluations, considering the phenological findings such as flowering and harvesting period, Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, and Centaurea cyanus were found to be suitable for Eskisehir climatic conditions due to their long flowering periods. On the other hand, if the stem length value, which is one of the most important parameters for cut flowers, is taken as a reference, the minimum stem length value of 30 cm and above is met by Zinnia elegans, Tagates erecta, Helianthus annuus, and Cleome spinosa while the vase life value of 6 days and above is met by Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, and Cleome spinosa. However, Cleome spinosa was not found to be suitable for the region due to its low yield value and short flowering period, while Zinnia elegans, Helianthus annuus, Tagates erecta, and Gomphrena globosa were found to be plants that could be evaluated for the region. In addition, it is believed that the cultivation of specialty cut flowers, with the selection of suitable species, will be an alternative production in regions without climatic advantages.
Subject(s)
Centaurea , Helianthus , Gardens , Turkey , FlowersABSTRACT
BACKGROUND: The primary challenge in the cut flower industry, specifically in the postharvest phase, is the short vase life of flowers. This issue, along with early leaf yellowing and perianth abscission, significantly diminishes the economic value of flowers due to their accelerated senescence. To tackle this, we conducted a factorial experiment on Alstroemeria cv. Rebecca, utilizing a completely randomized design with three replications. In this experiment the effects of varying concentrations of Salicylic acid (SA) (0, 1.5, and 3 mM) and sucrose (SU) (0% and 3%) were investigated on the postharvest quality of leaves and florets, with systematic evaluations every three days throughout their vase life. RESULTS: This experiment revealed that the specific treatment combination of 1.5 mM SA + 3% SU (T5) markedly improved various parameters, such as vase life, total chlorophyll content, membrane stability index, relative fresh weight, and water uptake of cut flowers. In our analysis, we observed that this preservative solution not only extended the vase life and enhanced water uptake but also effectively preserved total chlorophyll, mitigated the loss of fresh weight, and reduced membrane deterioration in petals. Additionally, our results showed an increase in the activities of catalase (CAT) and peroxidase (POD) enzymes, as well as total protein content, alongside a decrease in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels. Moreover, this study noted a decrease in microbial populations in solutions containing different concentrations of salicylic acid. CONCLUSIONS: Our research demonstrated that alstroemeria flowers maintained in a solution with 1.5 mM SA + 3% SU exhibited a significantly prolonged vase life of up to 21 days, in contrast to the 15 days observed in control flowers kept in water. These results are highly beneficial for manufacturers in the cut flower industry, as they provide a viable method to substantially extend the vase life of cut flowers. Such an enhancement in flower longevity can lead to increased market value and customer satisfaction. Furthermore, the reduction in flower senescence and decay rates can contribute to decreased waste and greater efficiency in cut flower distribution and sales, offering a substantial advantage to manufacturers in this competitive market. The extended vase life and reduced senescence observed in alstroemeria flowers treated with 1.5 mM SA and 3% SU are attributed to SA's role in enhancing endogenous defense responses and sucrose's function as an energy source, collectively improving water uptake, and delaying the natural decay process.
Subject(s)
Alstroemeria , Alstroemeria/metabolism , Sucrose/pharmacology , Salicylic Acid/pharmacology , Hydrogen Peroxide/pharmacology , Flowers/metabolism , Water/metabolism , ChlorophyllABSTRACT
Apixaban and dabigatran are the two major direct oral anticoagulant drugs to treat thromboembolic disordered patients. Increasing the clinical application for the thromboembolic disorder and monitoring the concentrations of apixaban, dabigatran, and their metabolites are essential in most clinical circumstances. In this work, we developed a rapid analytical methodology comprising of vortex-assisted salt-enhanced liquid-liquid microextraction technique coupled with UHPLC-MS/MS for the extraction and simultaneous determination of two major direct oral anticoagulant drugs (apixaban, dabigatran), and their two major metabolites from plasma, serum, and urine samples of patients. The developed method was optimized with various procedural steps and validated to study the analytical merits. The developed method yielded a good detection limit of 0.01 â¼ 0.37 ng/mL, 0.01 â¼ 0.32 ng/ml, and 0.01 â¼ 0.27 ng/mL for four target analytes in the plasma, serum, and urine matrices. Moreover, extraction recoveries ranged from 85.11 - 113.57% (for plasma), 89.63 - 110.47% (for serum), and 87.44 -106.79% (for urine samples) with 8.78% RSD. In addition, the method exhibited good R2 values of 0.999 for all four target analytes, and the specificity and carryover study revealed no carryover effect from the UHPLC-MS/MS system for determining the apixaban, dabigatran, and their metabolites. Due to the above advantages, the developed analytical technique was applied to examine 11 real-time clinical patients' samples, and the observed results were satisfactory for all three different sample matrices. Therefore, this analytical method can be applied for biomonitoring apixaban, dabigatran, and their two major metabolites with high sensitivity in a short time for various clinical applications.
Subject(s)
Dabigatran , Rivaroxaban , Humans , Dabigatran/analysis , Tandem Mass Spectrometry/methods , Biological Monitoring , Anticoagulants/therapeutic use , Anticoagulants/analysis , Chromatography, High Pressure Liquid/methodsABSTRACT
Disinfection byproducts (DBPs) are ubiquitous environmental contaminants, which are present in virtually all drinking water and linked to detrimental health effects. Iodinated-DBPs are more cytotoxic and genotoxic than chloro- and bromo-DBPs and are formed during disinfection of iodide-containing source water. Liquid-liquid extraction (LLE) paired with gas chromatography (GC)-mass spectrometry (MS) has been the method of choice in the study of low molecular weight iodinated-DBPs; however, this method is laborious and time-consuming and struggles with complex matrices. We developed an environmentally friendly method utilizing headspace solid phase extraction with the application of vacuum to measure six iodinated-trihalomethanes (I-THMs) in drinking water and urine. Vacuum-assisted sorbent extraction (VASE) has the ability to exhaustively and rapidly extract volatile and semivolatile compounds from liquid matrices without the use of solvent. Using VASE with GC-MS/MS provides improved analyte recovery and reduced matrix interference compared to LLE. Additionally, VASE enables extraction of 30 samples simultaneously with minimal sample handling and improved method reproducibility. Using VASE with GC-MS/MS, we achieved quantification limits of 3-4 ng/L. This technique was demonstrated on drinking water from four cities, where five I-THMs were quantified at levels 10-33 times below comparable LLE methods with 10 times lower volumes of sample (10 mL vs 100 mL).
Subject(s)
Disinfectants , Drinking Water , Water Pollutants, Chemical , Water Purification , Disinfection/methods , Drinking Water/analysis , Drinking Water/chemistry , Gas Chromatography-Mass Spectrometry , Tandem Mass Spectrometry , Reproducibility of Results , Trihalomethanes/analysis , Water Pollutants, Chemical/analysis , Water Purification/methods , Disinfectants/analysis , HalogenationABSTRACT
Botrytis cinerea (B. cinerea) causes gray mold disease (GMD), which results in physiological disorders in plants that decrease the longevity and economic value of horticultural crops. To prevent the spread of GMD during distribution, a rapid, early detection technique is necessary. Thermal imaging has been used for GMD detection in various plants, including potted roses; however, its application to cut roses, which have a high global demand, has not been established. In this study, we investigated the utility of thermal imaging for the early detection of B. cinerea infection in cut roses by monitoring changes in petal temperature after fungal inoculation. We examined the effects of GMD on the postharvest quality and petal temperature of cut roses treated with different concentrations of fungal conidial suspensions and chemicals. B. cinerea infection decreased the flower opening, disrupted the water balance, and decreased the vase life of cut roses. Additionally, the average temperature of rose petals was higher for infected flowers than for non-inoculated flowers. One day before the appearance of necrotic symptoms (day 1 of the vase period), the petal temperature in infected flowers was significantly higher, by 1.1 °C, than that of non-inoculated flowers. The GMD-induced increase in petal temperature was associated with the mRNA levels of genes related to ethylene, reactive oxygen species, and water transport. Furthermore, the increase in temperature caused by GMD was strongly correlated with symptom severity and fungal biomass. A multiple regression analysis revealed that the disease incidence in the petals was positively related to the petal temperature one day before the appearance of necrotic symptoms. These results show that thermography is an effective technique for evaluating changes in petal temperature and a possible method for early GMD detection in the cut flower industry.
ABSTRACT
The cut flower industry has a global reach as flowers are often produced in countries around the equator and transported by plane or ship (reefer) mostly to the global north. Vase-life issues are often regarded as linked to only postharvest conditions while cultivation factors are just as important. Here, we review the main causes for quality reduction in cut flowers with the emphasis on the importance of preharvest conditions. Cut flower quality is characterised by a wide range of features, such as flower number, size, shape, colour (patterns), fragrance, uniformity of blooming, leaf and stem colour, plant shape and developmental stage, and absence of pests and diseases. Postharvest performance involves improving and preserving most of these characteristics for as long as possible. The main causes for cut flower quality loss are reduced water balance or carbohydrate availability, senescence and pest and diseases. Although there is a clear role for genotype, cultivation conditions are just as important to improve vase life. The role of growth conditions has been shown to be essential; irrigation, air humidity, and light quantity and quality can be used to increase quality. For example, xylem architecture is affected by the irrigation scheme, and the relative humidity in the greenhouse affects stomatal function. Both features determine the water balance of the flowering stem. Light quality and period drives photosynthesis, which is directly responsible for accumulation of carbohydrates. The carbohydrate status is important for respiration, and many senescence related processes. High carbohydrates can lead to sugar loss into the vase water, leading to bacterial growth and potential xylem blockage. Finally, inferior hygiene during cultivation and temperature and humidity control during postharvest can lead to pathogen contamination. At the end of the review, we will discuss the future outlook focussing on new phenotyping tools necessary to quantify the complex interactions between cultivation factors and postharvest performance of cut flowers.
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Tree peony ( Paeonia suffruticosa Andr.) is a popular cut flower among ornamental plants. However, its short vase life severely hinders the production and application of cut tree peony flowers. To extend the postharvest longevity and improve the horticultural value, silver nanoparticles (Ag-NPs) was applied for reducing bacterial proliferation and xylem blockage in cut tree peony flowers in vitro and in vivo. Ag-NPs was synthesized with the leaf extract of Eucommia ulmoides and characterized. The Ag-NPs aqueous solution showed inhibitory activity against bacterial populations isolated from stem ends of cut tree peony 'Luoyang Hong' in vitro. The minimum inhibitory concentration (MIC) was 10 mg L-1. Compared with the control, pretreatments with Ag-NPs aqueous solution at 5 and 10 mg L-1 for 24 h increased flower diameter, relative fresh weight (RFW), and water balance of tree peony 'Luoyang Hong' flowers. Additionally, malondialdehyde (MDA) and H2O2 content in pretreated petals were lower than the control during the vase life. The activities of superoxide dismutase (SOD) and catalase (CAT) in pretreated petals were lower than that of the control at the early vase stage and higher at the late vase life. Furthermore, pretreatments with Ag-NPs aqueous solution at 10 mg L-1 for 24 h could reduce bacterial proliferation in the xylem vessels on the stem ends by confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM). Overall, pretreatments with green synthesized Ag-NPs aqueous solution effectively reduced bacteria-induced xylem blockage of cut tree peony, resulting in improved water uptake, extended vase life, and enhanced postharvest quality. Therefore, this technique can be used as a promising postharvest technology in the cut flower industry.
ABSTRACT
Vase-shaped microfossils (VSMs) are found globally in middle Neoproterozoic (800-730 Ma) marine strata and represent the earliest evidence for testate (shell-forming) amoebozoans. VSM tests are hypothesized to have been originally organic in life but are most commonly preserved as secondary mineralized casts and molds. A few reports, however, suggest possible organic preservation. Here, we test the hypothesis that VSMs from shales of the lower Walcott Member of the Chuar Group, Grand Canyon, Arizona, contain original organic material, as reported by B. Bloeser in her pioneering studies of Chuar VSMs. We identified VSMs from two thin section samples of Walcott Member black shales in transmitted light microscopy and used scanning electron microscopy to image VSMs. Carbonaceous material is found within the internal cavity of all VSM tests from both samples and is interpreted as bitumen mobilized from Walcott shales likely during the Cretaceous. Energy dispersive X-ray spectroscopy (EDS) and wavelength dispersive X-ray spectroscopy (WDS) reveal that VSM test walls contain mostly carbon, iron, and sulfur, while silica is present only in the surrounding matrix. Raman spectroscopy was used to compare the thermal maturity of carbonaceous material within the samples and indicated the presence of pyrite and jarosite within fossil material. X-ray absorption spectroscopy revealed the presence of reduced organic sulfur species within the carbonaceous test walls, the carbonaceous material found within test cavities, and in the sedimentary matrix, suggesting that organic matter sulfurization occurred within the Walcott shales. Our suite of spectroscopic analyses reveals that Walcott VSM test walls are organic and sometimes secondarily pyritized (with the pyrite variably oxidized to jarosite). Both preservation modes can occur at a millimeter spatial scale within sample material, and at times even within a single specimen. We propose that sulfurization within the Walcott Shales promoted organic preservation, and furthermore, the ratio of iron to labile VSM organic material controlled the extent of pyrite replacement. Based on our evidence, we conclude that the VSMs are preserved with original organic test material, and speculate that organic VSMs may often go unrecognized, given their light-colored, translucent appearance in transmitted light.
Subject(s)
Iron , Spectrum Analysis, Raman , Arizona , Spectrum Analysis, Raman/methodsABSTRACT
Introduction: Hyperspectral imaging (HSI) and deep learning techniques have been widely applied to predict postharvest quality and shelf life in multiple horticultural crops such as vegetables, mushrooms, and fruits; however, few studies show the application of these techniques to evaluate the quality issues of cut flowers. Therefore, in this study, we developed a non-contact and rapid detection technique for the emergence of gray mold disease (GMD) and the potential longevity of cut roses using deep learning techniques based on HSI data. Methods: Cut flowers of two rose cultivars ('All For Love' and 'White Beauty') underwent either dry transport (thus impaired cut flower hydration), ethylene exposure, or Botrytis cinerea inoculation, in order to identify the characteristic light wavelengths that are closely correlated with plant physiological states based on HSI. The flower bud of cut roses was selected for HSI measurement and the development of a vase life prediction model utilizing YOLOv5. Results and discussion: The HSI results revealed that spectral reflectance between 470 to 680 nm was strongly correlated with gray mold disease (GMD), whereas those between 700 to 900 nm were strongly correlated with flower wilting or vase life. To develop a YOLOv5 prediction model that can be used to anticipate flower longevity, the vase life of cut roses was classed into two categories as over 5 d (+5D) and under 5 d (-5D), based on scoring a grading standard on the flower quality. A total of 3000 images from HSI were forwarded to the YOLOv5 model for training and prediction of GMD and vase life of cut flowers. Validation of the prediction model using independent data confirmed its high predictive accuracy in evaluating the vase life of both 'All For Love' (r2 = 0.86) and 'White Beauty' (r2 = 0.83) cut flowers. The YOLOv5 model also accurately detected and classified GMD in the cut rose flowers based on the image data. Our results demonstrate that the combination of HSI and deep learning is a reliable method for detecting early GMD infection and evaluating the longevity of cut roses.
ABSTRACT
As a commercial high-grade cut flower, the marketability of herbaceous peony (Paeonia lactiflora Pall.) is limited by its short vase life in water. Si (silicon) is an alternative to improve the postharvest life of cut flowers. However, the effects of the combined application of Si and preservatives on the postharvest performance of cut peony flowers are unknown. In this study, the effects of a Si application and a preservative alone and collegial on the longevity of the vase life, water loss, antioxidant defense system, and stock carbohydrates level of cut flowers of three peony cultivars were investigated. It was observed that Si effectively prolonged the vase life, while the preservative alone, to a lesser extent, but markedly induced an early flowering and a greater flower diameter (flower open degree). The simultaneous use of Si and the preservatives not only showed larger flowers, but also improved the postharvest performance as characterized by an extended vase life and delayed the water loss. In addition, the Si supplementation dramatically intensified the antioxidant defense system (ameliorated antioxidant enzymes and alleviated ROS accumulation) in petals but did not increase the stock carbohydrates (starch and soluble sugars) levels, as compared to the treatment with the preservative alone. We show that a Si supplementation to a preservative is highly recommended for a large-scale use to promote the postharvest performance and competitiveness of marketed cut flowers.
Subject(s)
Paeonia , Silicon/pharmacology , Antioxidants/pharmacology , Flowers , Sugars/pharmacology , Water/pharmacologyABSTRACT
The bird of paradise (Strelitzia reginae L.) is one of the important tropical cut flowers. Generally, flowers like bird of paradise (BOP) grown for the commercial ornamental market must be of high pre and postharvest quality. Thus, to improve the postharvest longevity and increase marketability, the relative efficacy of two different biologically synthesized nanoparticles (NPs) was evaluated. The novel proprietary stimulants were graphene oxide (GO) and silver nanoparticles (SNPs). The NP treatments were applied as a vase (lower concentrations) solutions. Among all the applied treatments, the synergistic effect of GO + SNPs at 1 µL L-1 vase solution significantly (p =0.05) prolongs the post-harvest life of cut flowers of BOP. Increased vase life over the deionized water (DI) control was associated with better maintenance of relative water uptake, relative fresh weight, suppressed microbial density at stem-end and delay of stem blockage, reduced electrolyte leakage, malondialdehyde (MDA), SOD, and POD activity. In contrast to control, administration of NPs gave better results for all analyzed parameters. Application of biologically synthesized NPs in combination (GO + SNPs at 1 µL L-1) extended the vase life of cut flowers by 6 days compared with control flowers, and overall, showed better results than the control. The findings of the studies revealed that the standardized NPs could have more potential in prolonging the postharvest life of cut flowers in BOP. Thus, this technique can be used as a novel postharvest technology for commercial application in cut flowers.
ABSTRACT
The vase life of cut rose is relatively short, therefore; preserving its postharvest quality via eco-friendly approaches is of particular economic importance. From the previous literature, despite melatonin (MT) plays diverse important roles in the postharvest quality maintenance, its impact on preserving the postharvest quality of cut flowers is really scarce. This research therefore was undertaken to find out the possibility of exogenous MT as an eco-friendly preservative to extend the vase life of cut roses. The flowering stems of Rosa hybrida cv. 'First Red' were pulsed in MT solutions at 0, 0.1, 0.2 and 0.3 mM for 30 min and then transferred to distilled water for evaluation. The vase life was significantly prolonged and relative water content was considerably maintained due to MT application compared to the control, more so with 0.2 mM concentration which nearly doubled the vase life (1.9-fold) higher than the control. SEM investigation showed that MT treatment reduced the stomatal aperture in lower epidermis which was widely opened in control flowers. MT treatment significantly increased the phenol content, glutathione (GSH) content and CAT, APX and GR enzyme activities compared to untreated flowers. Additionally, the radical scavenging capacity in MT-treated flowers was considerably higher than that of control and therefore MT treatment reduced H2O2 production and lipid peroxidation, which altogether reflected in membrane stability maintenance.
ABSTRACT
BACKGROUND: Environmental factors seem to influence clinical manifestations of sickle cell disease (SCD), but few studies have shown consistent findings. We conducted a retrospective multicentric observational study to investigate the influence of environmental parameters on hospitalization for vaso-occlusive crises (VOC) or acute chest syndrome (ACS) in children with SCD. METHODS: Hospital admissions were correlated with daily meteorological and air-quality data obtained from Environmental Regional Agencies in the period 2011-2015. The effect of different parameters was assessed on the day preceding the crisis up to ten days before. Statistical analysis was performed using a quasi-likelihood Poisson regression in a generalized linear model. RESULTS: The risk of hospitalization was increased for low maximum temperature, low minimum relative humidity, and low atmospheric pressure and weakly for mean wind speed. The diurnal temperature range and temperature difference between two consecutive days were determined to be important causes of hospitalization. For air quality parameters, we found a correlation only for high levels of ozone and for low values at the tail corresponding to the lowest concentration of this pollutant. CONCLUSIONS: Temperature, atmospheric pressure, humidity and ozone levels influence acute complications of SCD. Patients' education and the knowledge of the modes of actions of these factors could reduce hospitalizations.
ABSTRACT
Endophytic bacteria improve the growth, physiology, and metabolite profile of plants. They are known as potential biocontrol agents of soil-borne diseases. This study evaluated the effects of endophytic bacterial strains on growth, vase life, biochemical attributes, and antioxidant and nematicidal activities of French marigold (Tagetes patula). French marigold seeds were sole and consortium inoculated with three promising endophytic bacterial strains, Burkholderia phytofirmans (PsJN), Enterobacter sp. (MN17), and Bacillus sp. (MN54). The vase life of French marigold was promoted by 66.6% in the individual application of PsJN and 100% in plants treated with consortium compared to the uninoculated control. The shoot and root fresh weights were also increased by 65.9 and 68.7%, with the combined application of all three strains. The total phenolics, flavonoid, and protein contents were higher in consortium treatment with an increase of up to 38.0, 55.9, and 65.9%, respectively, compared to the uninoculated control. Furthermore, combined application of endophytic bacterial strains promoted DPPH radical scavenging, mortality of plant-parasitic nematodes, and ferric reducing antioxidant power activities with increase of up to 278.0, 103.8, and 178.0%, respectively, compared to uninoculated control. An increase in antioxidant activities of ascorbate peroxidase (APX), catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD) were observed up to 77.3, 86.0, 91.6, and 102.9%, respectively by combined application of endophytic bacterial strains. So, given the economic importance of floriculture crops, endophytic bacterial isolates studied here have shown a great potential for improving the productivity of cultivated ornamental French marigold.