Combining slow-release fertilizer and plastic film mulching reduced the carbon footprint and enhanced maize yield on the Loess Plateau.

Agricultural practices significantly contribute to greenhouse gas (GHG) emissions, necessitating cleaner production technologies to reduce environmental pressure and achieve sustainable maize production. Plastic film mulching is commonly used in the Loess Plateau region. Incorporating slow-release fertilizers as a replacement for urea within this practice can reduce nitrogen losses and enhance crop productivity. Combining these techniques represents a novel agricultural approach in semi-arid areas. However, the impact of this integration on soil carbon storage (SOCS), carbon footprint (CF), and economic benefits has received limited research attention. Therefore, we conducted an eight-year study (2015-2022) in the semi-arid northwestern region to quantify the effects of four treatments [urea supplied without plastic film mulching (CK-U), slow-release fertilizer supplied without plastic film mulching (CK-S), urea supplied with plastic film mulching (PM-U), and slow-release fertilizer supplied with plastic film mulching (PM-S)] on soil fertility, economic and environmental benefits. The results revealed that nitrogen fertilizer was the primary contributor to total GHG emissions (≥71.97%). Compared to other treatments, PM-S increased average grain yield by 12.01%-37.89%, water use efficiency by 9.19%-23.33%, nitrogen accumulation by 27.07%-66.19%, and net return by 6.21%-29.57%. Furthermore, PM-S decreased CF by 12.87%-44.31% and CF per net return by 14.25%-41.16%. After eight years, PM-S increased SOCS (0-40 cm) by 2.46%, while PM-U decreased it by 7.09%. These findings highlight the positive effects of PM-S on surface soil fertility, economic gains, and environmental benefits in spring maize production on the Loess Plateau, underscoring its potential for widespread adoption and application.

Clinical usefulness and acceptability of small-bowel capsule endoscopy with panoramic imaging compared with axial imaging in Japanese patients.

Objectives: We aimed to evaluate the usefulness and acceptability of CapsoCam Plus (CapsoCam) in Japanese patients. Methods: This retrospective single-center study enrolled 930 patients with suspected small-bowel bleeding (SSBB) who underwent capsule endoscopy. Thirty-three patients using CapsoCam and PillCam SB3 (SB3) were matched using propensity score matching. The diagnostic yield and the acceptability of CapsoCam were evaluated. Results: There was no SSBB case where capsule endoscopy was performed within 48 h of bleeding. CapsoCam had a significantly higher observation rate of the entire small bowel (97% vs. 73%, p = 0.006) and Vater's papilla (82% vs. 15%, p < 0.001) than SB3. The reading time of CapsoCam was significantly longer than that of SB3 (30 vs. 25 min, p < 0.001), and CapsoCam's time from the capsule endoscopy swallowing to read completion was longer than that of SB3 (37 vs. 12 h, p < 0.001). The two groups showed no difference in the capsule endoscopy findings according to the P classification. Notably, 85% of the patients using CapsoCam reported examination distress as "not at all" or "almost not," and 94% reported swallowing difficulty as "very easy" or "easy." Conclusions: CapsoCam took time to read; however, it is a well-tolerated examination with a high observation rate of Vater's papilla and entire small-bowel mucosa. Detectability of bleeding sources was comparable in both modalities for cases of occult SSBB and overt SSBB more than 48 h after bleeding. CapsoCam is a useful modality for patients with SSBB.

Suppression of SlHDT1 expression increases fruit yield and decreases drought and salt tolerance in tomato.

Histone deacetylation, one of most important types of post-translational modification, plays multiple indispensable roles in plant growth and development and abiotic stress responses. However, little information about the roles of histone deacetylase in regulating inflorescence architecture, fruit yield, and stress responses is available in tomato. Functional characterization revealed that SlHDT1 participated in the control of inflorescence architecture and fruit yield by regulating auxin signalling, and influenced tolerance to drought and salt stresses by governing abscisic acid (ABA) signalling. More inflorescence branches and higher fruit yield, which were influenced by auxin signalling, were observed in SlHDT1-RNAi transgenic plants. Moreover, tolerance to drought and salt stresses was decreased in SlHDT1-RNAi transgenic lines compared with the wild type (WT). Changes in parameters related to the stress response, including decreases in survival rate, chlorophyll content, relative water content (RWC), proline content, catalase (CAT) activity and ABA content and an increase in malonaldehyde (MDA) content, were observed in SlHDT1-RNAi transgenic lines. In addition, the RNA-seq analysis revealed varying degrees of downregulation for genes such as the stress-related genes SlABCC10 and SlGAME6 and the pathogenesis-related protein P450 gene SlCYP71A1, and upregulation of the pathogenesis-related protein P450 genes SlCYP94B1, SlCYP734A7 and SlCYP94A2 in SlHDT1-RNAi transgenic plants, indicating that SlHDT1 plays an important role in the response to biotic and abiotic stresses by mediating stress-related gene expression. In summary, the data suggest that SlHDT1 plays essential roles in the regulation of inflorescence architecture and fruit yield and in the response to drought and salt stresses.

Kinetic modelling of UVC and UVC/H2O2 oxidation of an aqueous mixture of antibiotics in a completely mixed batch photoreactor.

The removal kinetics of an aqueous mixture of thirteen antibiotics (i.e., ampicillin, cefuroxime, ciprofloxacin, flumequine, metronidazole, ofloxacin, oxytetracycline, sulfadimethoxine, sulfamethoxazole, sulfamethazine, tetracycline, trimethoprim and tylosin) by batch UVC and UVC/H2O2 processes has been modeled in this work. First, molar absorption coefficients (ε), direct quantum yields (Φ) and the rate constants of the reaction of antibiotics with hydroxyl radical (kHO•) (model inputs) were determined for each antibiotic and compared with literature data. The values of these parameters range from 0.3 to 21.8 mM-1 cm-1 for ε, < 0.01 to 67.8 mmol·E-1 for Φ and 3.8 × 109 to 1.7 × 1010 M-1 s-1 for kHO•. Second, a regression model was developed to compute the rate constants of the reactions of the antibiotics with singlet oxygen (k1O2) from experimental data obtained in batch UVC experiments treating a mixture of the antibiotics. k1O2 values in the 1-50 × 106 M-1 s-1 range were obtained for the antibiotics studied. Finally, a semi-empirical kinetic model comprising a set of ordinary differential equations was solved to simulate the evolution of the residual concentration of antibiotics and hydrogen peroxide (model outputs) in a completely mixed batch photoreactor. Model predictions were reasonably consistent with the experimental data. The kinetic model developed might be combined with computational fluid dynamics to predict process performance and energy consumption in UVC and UVC/H2O2 applications at full scale.

Rootstock selection for 'Swatow' Mandarin trees grown at different locations throughout the Brazilian subtropics.

Evaluating citrus rootstocks is of paramount importance in determining their suitability for a certain region and promoting resilience in orchards by increasing the genetic pool, thereby potentially contributing to a more strategic establishment of new plantings. This long-term field study (2000-2013) aimed to evaluate different rootstocks for 'Swatow' mandarin grown at two locations (Paranavaí and Londrina) in the Brazilian subtropics. Nine rootstocks were evaluated, including 'Rangpur' lime, 'Swingle' citrumelo, 'Volkamer' lemon, 'Caipira DAC' sweet orange, 'Cleopatra' and 'Sunki' mandarins, 'Trifoliate' orange, 'Carrizo', and 'Fepagro C-13' citranges. Trees were assessed for vegetative growth, yield, fruit quality, density, and yield estimates. The experimental design was a randomized block arranged in a 9 × 2 setting (rootstock × location) with 6 replicates and 4 trees per plot. 'Swatow' trees grew more vigorously in Londrina than Paranavaí, particularly for 'Cleopatra' and 'Sunki' pairings. Tree vigor was reduced with 'Trifoliate', resulting in higher tree density estimates and yield efficiency. This rootstock, along with 'Rangpur', 'Swingle', and 'Carrizo' provided superior yield to the scion. All tested rootstocks conferred good fruit quality. Fruits were larger and heavier in 'Sunki' pairings, showing higher soluble solids (SS) content, along with 'Caipira DAC', 'Trifoliate', 'Swingle', and 'Carrizo' at both locations. Our findings confirm the suitability of 'Trifoliate' orange, 'Carrizo' citrange, or 'Caipira DAC' orange rootstocks as promising candidates for 'Swatow' mandarin cultivation in humid subtropical and analogous regions. Further investigations are invoked to improve the horticultural performance of 'Swatow' mandarin trees grafted onto these rootstocks.

The impact of salinization on soil bacterial diversity, yield and quality of Glycyrrhiza uralensis Fisch.

Soil salinization seriously affects soil microbial diversity, and crop yield and quality worldwide. Microorganisms play a vital role in the process of crop yield and quality. Traditional Chinese medicine Glycyrrhiza uralensis Fisch. (licorice) can grow tenaciously in the heavily salinized land. However, the relationship between licorice plants and soil microorganisms is not clear. A field experiment was carried out to explore the effects of three different degrees of salinized soils on (i) licorice crop performance indicators, (ii) soil physical and chemical properties, and (iii) the changes in soil bacterial community structure and functional diversity in a semi-arid area of northwest China. The results showed that with the aggravation of soil salinization, the licorice yield, soil nutrients, and the bacterial abundance of Gemmatimonadetes and Myxococcota showed a downward trend, while the concentration of glycyrrhizic acid and liquiritin, and the bacterial abundance of Actinobacteria and Firmicutes showed an upward trend. The change of licorice yield mainly depended on the soil physical and chemical properties (e.g., EC and alkaline hydrolysable nitrogen). The change of licorice quality was more closely related to the change of bacterial diversity. The effect of bacterial diversity on liquiritin was greater than that on glycyrrhizic acid. Among them, Gemmatimonadetes were significantly negatively correlated with liquiritin and glycyrrhizic acid. These findings suggest that the increased soil Actinobacteria and Firmicutes or reduced Gemmatimonadetes and Myxococcota may provide a healthy and suitable living condition for the sustainable development of medicinal plant crops in a salinized soil ecosystem.

Enhancing rice yield prediction: a deep fusion model integrating ResNet50-LSTM with multi source data.

Rice production is pivotal for ensuring global food security. In Pakistan, rice is not only the dominant Kharif crop but also a significant export commodity that significantly impacts the state's economy. However, Pakistan faces challenges such as abrupt climate change and the COVID-19 pandemic, which affect rice production and underscore the need for predictive models for informed decisions aimed at improving productivity and ultimately the state's economy. This article presents an innovative deep learning-based hybrid predictive model, ResNet50-LSTM, designed to forecast rice yields in the Gujranwala district, Pakistan, utilizing multi-modal data. The model incorporates MODIS satellite imagery capturing EVI, LAI, and FPAR indices along with meteorological and soil data. Google Earth Engine is used for the collection and preprocessing of satellite imagery, where the preprocessing steps involve data filtering, applying region geometry, interpolation, and aggregation. These preprocessing steps were applied manually on meteorological and soil data. Following feature extraction from the imagery data using ResNet50, the three LSTM model configurations are presented with distinct layer architectures. The findings of this study exhibit that the model configuration featuring two LSTM layers with interconnected cells outperforms other proposed configurations in terms of prediction performance. Analysis of various feature combinations reveals that the selected feature set (EVI, FPAR, climate, and soil variables) yields highly accurate results with an R2 = 0.9903, RMSE = 0.1854, MAPE = 0.62%, MAE = 0.1384, MRE = 0.0062, and Willmott's index of agreement = 0.9536. Moreover, the combination of EVI and FPAR is identified as particularly effective. Our findings revealed the potential of our framework for globally estimating crop yields through the utilization of publicly available multi-source data.

Genomic prediction of yield-related traits and genome-based establishment of heterotic pattern in maize hybrid breeding of Southwest China.

When genomic prediction is implemented in breeding maize (Zea mays L.), it can accelerate the breeding process and reduce cost to a large extent. In this study, 11 yield-related traits of maize were used to evaluate four genomic prediction methods including rrBLUP, HEBLP|A, RF, and LightGBM. In all the 11 traits, rrBLUP had similar predictive accuracy to HEBLP|A, and so did RF to LightGBM, but rrBLUP and HEBLP|A outperformed RF and LightGBM in 8 traits. Furthermore, genomic prediction-based heterotic pattern of yield was established based on 64620 crosses of maize in Southwest China, and the result showed that one of the parent lines of the top 5% crosses came from temp-tropic or tropic germplasm, which is highly consistent with the actual situation in breeding, and that heterotic pattern (Reid+ × Suwan+) will be a major heterotic pattern of Southwest China in the future.

Sodium stress-induced oxidative damage and antioxidant responses during grain filling in Indica rice.

KEY MESSAGE: Sodium treatment caused the sodium ion accumulation at the milk stage of immature rice grains which in turn triggered the overproduction of reactive oxygen species and oxidative damage. The tolerant cultivar showed an enhanced antioxidative response and induced expressions of OsNHX and OsHKT ion-transporters. Sodium chloride-(NaCl) induced soil salinity is a major constraint hindering global rice production. Amongst its constituent ions, sodium (Na+) is known to be the main driver of toxicity under salt stress. The present investigation aims to measure the impacts of excess Na+ during rice grain filling using two Indica rice cultivars with opposite tolerances to salt (salt tolerant: Panvel-3, salt-sensitive: Sahyadri-3) mainly via oxidative and responsive antioxidative pathways. Plants were treated with Na+-specific treatments and NaCl with equimolar Na+ levels (100 mM) at the initiation of the reproductive phase. Stressed and control plants were harvested at three different grain-filling stages- early milk, milk, and dough and assessed for ion accumulation and oxidative damage/antioxidant responses under Na+ stress. Na+ toxicity triggered reactive oxygen species (ROS) production and upregulated the responsive enzymatic antioxidants. Na+ stress also increased the nitric oxide (NO) levels and the activity of nitrate reductase in immature grains. Differential expression levels of OsNHX and OsHKT transporters were observed in response to Na+ stress. Mature grains displayed a high accumulation of Na+ along with reduced K+ content and elevated Na+/K+ under high Na+ availability. The alterations in mature grains' sugar, starch, and protein content were also observed in response to the Na+ stress. Overall, the salt-tolerant cultivar displayed higher antioxidant activities and a lower rate of ROS generation in response to the Na+ stress. Results suggested a link between Na+ accumulation, Na+-mediated stress responses via anti/-oxidant pathways, and the grain-filling process in both rice cultivars.

Common cocklebur (Xanthium strumarium L.) interference in grain sorghum [Sorghum bicolor (L.) Moench]: the influence of weed and crop density.

BACKGROUND: The common cocklebur (Xanthium strumarium L.) is an invasive weed species in the Asteraceae family that probably originated in Central or South America but has now spread worldwide, where it infests numerous crop fields, including sorghum. It is also a significant invasive weed in various parts of Ethiopia, including the eastern region. In this study field experiments were conducted to investigate the effect of various densities of sorghum and X. strumarium on their growth and reproductive output at Haramaya and Babile Research Stations of Haramaya University in Eastern Ethiopia during the 2022/2023 growing seasons. RESULTS: Sorghum yield loss was greatly affected by X. strumarium density, reaching maximum yield losses of 79.2% and 93.1% at the maximum weed density at Haramaya and Babile, respectively. The presence of X. strumarium in sorghum resulted in reduced aboveground dry matter and leaf area index (LAI). The extent of this reduction depended on the density of X. strumarium. As crop density increased, X. strumarium dry matter, LAI, and bur production m-2 decreased. The highest bur production per unit area for X. strumarium was observed at its highest density (16 plants m-2) with 1097 and 869 burs per unit area at Haramaya and Babile, respectively. CONCLUSION: These results indicated that higher densities of sorghum were effective in suppressing the bur production of this weed, leading to reduced yield loss. Therefore, sorghum competitiveness against X. strumarium can be improved using higher crop densities. This could play a key role in weed management by reducing the use of herbicides and mechanical controls, thereby forming an important part of integrated weed management. © 2024 Society of Chemical Industry.

Insect frass fertilizer as a regenerative input for improved biological nitrogen fixation and sustainable bush bean production.

Bush bean (Phaseolus vulgaris L.) production is undermined by soil degradation and low biological nitrogen fixation (BNF) capacity. This study evaluated the effect of black soldier fly frass fertilizer (BSFFF) on bush bean growth, yield, nutrient uptake, BNF, and profitability, in comparison with commercial organic fertilizer (Phymyx, Phytomedia International Ltd., Kiambu, Kenya), synthetic fertilizer (NPK), and rhizobia inoculant (Biofix, MEA Fertilizers, Nairobi, Kenya). The organic fertilizers were applied at rates of 0, 15, 30, and 45 kg N ha-1 while the NPK was applied at 40 kg N ha-1, 46 kg P ha-1, and 60 kg K ha-1. The fertilizers were applied singly and in combination with rhizobia inoculant to determine the interactive effects on bush bean production. Results showed that beans grown using BSFFF were the tallest, with the broadest leaves, and the highest chlorophyll content. Plots treated with 45 kg N ha-1 BSFFF produced beans with more flowers (7 - 8%), pods (4 - 9%), and seeds (9 - 11%) compared to Phymyx and NPK treatments. The same treatment also produced beans with 6, 8, and 18% higher 100-seed weight, compared to NPK, Phymyx, and control treatments, respectively. Beans grown in soil amended with 30 kg N ha-1 of BSFFF had 3-14-fold higher effective root nodules, fixed 48%, 31%, and 91% more N compared to Phymyx, NPK, and rhizobia, respectively, and boosted N uptake (19 - 39%) compared to Phymyx and NPK treatments. Application of 45 kg N ha-1 of BSFFF increased bean seed yield by 43%, 72%, and 67% compared to the control, NPK and equivalent rate of Phymyx, respectively. The net income and gross margin achieved using BSFFF treatments were 73 - 239% and 118 - 184% higher than the values obtained under Phymyx treatments. Our findings demonstrate the high efficacy of BSFFF as a novel soil input and sustainable alternative for boosting BNF and improving bush bean productivity.

Methane-derived microbial biostimulant reduces greenhouse gas emissions and improves rice yield.

Introduction: More than half of the world's population consumes rice as their primary food. The majority of rice production is concentrated in Asia, with the top 10 rice-growing countries accounting for 84% of the world's total rice cultivation. However, rice production is also strongly linked to environmental changes. Among all the global sources of greenhouse gas (GHG) emissions, paddy cultivation stands out as a significant contributor to global methane (CH4) and nitrous oxide (N2O) emissions. This contribution is expected to increase further with the projected increase of 28% in global rice output by 2050. Hence, modifications to rice management practices are necessary both to increase yield and mitigate GHG emissions. Methods: We investigated the effect of seedling treatment, soil application, and foliar application of a methane-derived microbial biostimulant on grain yield and GHG emissions from rice fields over three seasons under 100% fertilizer conditions. Further, microbial biostimulant was also tested under 75% nitrogen (N) levels to demonstrate its effect on grain yield. To understand the mechanism of action of microbial biostimulant on crop physiology and yield, a series of physiological, transcript, and metabolite analyses were also performed. Results: Our three-season open-field studies demonstrated a significant enhancement of grain yield, up to 39%, with a simultaneous reduction in CH4 (31%-60%) and N2O (34%-50%) emissions with the use of methane-derived microbial biostimulant. Under 75% N levels, a 34% increase in grain yield was observed with microbial biostimulant application. Based on the physiological, transcript, and metabolite analyses data, we were further able to outline the potential mechanisms for the diverse synergistic effects of methane-derived microbial biostimulant on paddy, including indole-3-acetic acid production, modulation of photosynthesis, tillering, and panicle development, ultimately translating to superior yield. Conclusion: The reduction in GHG emission and enhanced yield observed under both recommended and reduced N conditions demonstrated that the methane-derived biostimulant can play a unique and necessary role in the paddy ecosystem. The consistent improvements seen across different field trials established that the methane-derived microbial biostimulant could be a scalable solution to intensify rice productivity with a lower GHG footprint, thus creating a win-win-win solution for farmers, customers, and the environment.

Investigating the impact of long-term bristlegrass coverage on rhizosphere microbiota, soil metabolites, and carbon-nitrogen dynamics for pear agronomic traits in orchards.

Background: Grass coverage (GC) under no-tillage systems in orchards signifcantly infuences underground carbon (C) and nitrogen (N) sequestration, primarily through promoting mineral nutrient utilization by rhizospheric microorganisms. However, the comprehensive impact of GC on microbial communities and plant responses using soil metabolomics remains inadequately recognized. Methods: We investigated two rhizosphere types established since 2002: bristlegrass (Setaria viridis (L.) P. Beauv.) coverage (SC) and clean cultivation (CC) to assess their efects on soil parameters, enzyme activities, and key pear agronomic traits, including yield (single fruit weight (SFW)) and qualities (soluble solids content (SSC), and total soluble sugar (TSS)). We combined microbiological analysis (16S rRNA sequencing) and non-targeted metabolomics (UPLC-MS/MS and GC-MS) to explore how microbial communities infuence fruit agronomic traits and soil nutrient dynamics in pear orchards under SC conditions. Results: Our fndings indicate that SC signifcantly enhances soil organic carbon (SOC), soil organic nitrogen (SON), the C:N ratio, and available nitrogen (AN). Moreover, SC leads to pronounced increases in soil enzyme activities involved in the C cycle and storage, including soil sucrase, ß-glucosidase, polyphenol oxidase and cellulase. Microbiome analysis revealed substantial diferences in microbial community composition and diversity indices between SC and CC rhizosphere soils within the 0-40 cm depth. Metabolomic analysis demonstrated significant alterations in metabolite profiles across both the 0-20 cm and 20-40 cm layers under SC conditions. The identifed metabolites primarily involve sugar and amino acid-related metabolic pathways, refecting perturbations in C and N metabolism consistent with shifts in bacterial community structure. Several plant growth-promoting rhizobacteria (PGPRs) taxa (e.g., Haliangium, Bacteroides, mle1-7, Subgroup_22, Ellin6067, MND1, Flavobacterium, and Cellvibrio) were enriched under SC, associated with metabolites such as sucrose, N-acetyl-D-glucosamine, N-acetyl-L-glutamic acid, rhamnose, UDP-GlcNAc and D-maltose. These fndings suggest their roles in promoting C and N sequestration processes through sucrose synthesis and glycolytic pathways in the soil, which was signifcantly correlated with the formation of agronomic traits such as fruit yield, SFW SSC and TSS (p<0.05), and SC treatments signifcantly increased yields by 35.40-62.72% and sucrose content in TSS by 2.43-3.96 times than CC treatments. Conclusion: This study provides valuable insights into the efects of SC on soil microbial communities and plant physiology, enhancing our understanding of their implications for sustainable orchard management.

Simultaneous improvement of fiber yield and quality in upland cotton (Gossypium hirsutum L.) by integration of auxin transport and synthesis.

Cotton is a widely planted commercial crop in the world. Enhancing fiber yield and quality is a long-term goal for cotton breeders. Our previous work has demonstrated that fine promotion of auxin biosynthesis in ovule epidermis, by overexpressing FBP7pro::iaaM, has a significant improvement on lint yield and fiber fineness. Lately, transgenic cottons overexpressing GhROP6 variants modify mature fiber length by controlling GhPIN3a-mediated polar auxin transport in ovules. Here, this study showed that all these GhROP6-related cottons displayed unsatisfactory agronomic performance in field conditions. Yet extra auxin supply could promote their fiber development, suggesting inadequate auxin supply in the ovules. Thus, these cottons were integrated with enhanced auxin synthesis by crossing with FBP7pro::iaaM cotton. All the transgene-stacked cottons exhibited synergetic effects on cotton yield (seedcotton yield, lint yield, and lint percentage) and quality (length, strength, and micronaire). Notably, comparing to the FBP7pro::iaaM background, the transgene-stacked cotton co-expressing FBP7pro::iaaM and CA-ghrop6 (constitutively active GhROP6) exhibited a 12.6% increase in seedcotton yield and a 19.0% increase in lint yield over a three-year field trial, and simultaneously resulted in further improvement on fiber length, strength, and micronaire. Collectively, our data provide a potential strategy for genetic improvement on cotton fiber yield and quality. Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-024-01500-w.

Higher sensitivity of pericardial fluid cytology than biopsy in malignant effusions with potential explanation of false-negative cytology: A multi-institutional analysis.

OBJECTIVE: Malignant pericardial effusions are associated with a poor prognosis. Pericardial fluid cytology and pericardial biopsy are the primary methods for diagnosis. This study aimed to conduct a multi-institutional analysis to compare the diagnostic sensitivity of cytology and biopsy, and to investigate potential explanations for false-negative results in cytology. METHODS: A retrospective review of pericardial fluid cytology cases with concurrent biopsy was conducted across four different institutions. Results were compared using standard statistical methods with attention to sensitivity and histologic distribution. False-negative cytology cases were investigated for further exploration. RESULTS: A total of 309 cases were collected, of which 99 (32.0%) were confirmed malignant through repeat sampling or clinical history. Pericardial fluid cytology and biopsy identified 84 and 64 malignant cases, respectively. Our findings confirmed significantly higher sensitivity of cytology compared to biopsy (84.8% vs 65.7%). The most common sites of origin were lung, breast, and gastrointestinal, with adenocarcinoma being the most prevalent histologic subtype. Histologic review of 12 false-negative cytology cases revealed three key explanations; lymphoma was the most common missed diagnosis (33.3%); fibrinous pericarditis obscures neoplastic cells on the pericardial surface; and pericardial involvement can be seen without extension into the pericardial space. CONCLUSION: This study demonstrated diagnostic superiority of pericardial fluid cytology over biopsy in the evaluation of malignant pericardial effusions. We identified several limitations in fluid cytology causing false negatives. In the context of an underlying malignancy with pericardial effusion, pathologists should consider immunohistochemistry studies to aid on the diagnosis.

Biochar improves the nutrient cycle in sandy-textured soils and increases crop yield: a systematic review.

BACKGROUND: Biochar is a relatively new development in sustainable agricultural management that can be applied to ameliorate degraded and less fertile soils, especially sandy-textured ones, to improve their productivity with respect to crop production through improved nutrient availability. However, as the literature has shown, the response of sandy-textured soils to biochar varies in terms of effect size and direction. Therefore, the present study systematically reviewed the available evidence to synthesize the impact of biochar amendments on aspects of the nutrient cycle of sandy-textured soils. METHODS: Both peer-reviewed and gray literature were searched in English in bibliographic databases, organizational web pages, and Internet search engines. Articles underwent a two-stage screening (title and abstract, and full-text) based on predefined criteria, with consistency checks. Validity assessments were conducted, utilizing specifically designed tools for study validity. Data extraction involved categorizing the various properties of the nutrient cycle into nine main Soil and Plant Properties (SPPs), each of which was studied independently. Nine meta-analyses were performed using a total of 1609 observations derived from 92 articles. Comparing meta-averages with and without correction for publication bias suggests that publication bias plays a minor role in the literature, while some indication for publication bias is found when accounting for heterogeneity by means of meta-regressions. REVIEW FINDINGS: According to the results, soil total and available nitrogen [N], phosphorous [P] and potassium [K], plant nutrient level, and potential cation exchange capacity (CEC) increased by 36% (CI [23%, 50%]), 34% (CI [15%, 57%]), 15% (CI [1%, 31%]), and 18% (CI [3%, 36%), respectively, and N2O emission and mineral nutrient leaching decreased by 29% (CI [- 48%, - 3%]) and 38% (CI [- 56%, - 13%). On average, however, biochar had no effect on soil mineral nitrogen and nutrient use efficiency. Publication bias was identified in the response of effective CEC. After corrections for publication bias, the response shifted from 36% to a negative value of - 34% (CI [- 50%, - 14%]). Meta-regression found that the effect modifiers experimental continent, biochar application rate, and soil pH, explain result heterogeneity. Stronger responses came from the continent of South America, higher application rates, and higher pH soils. Overall, biochar is found useful for many SPPs of nutrient cycling of sandy-textured soils, thereby contributing to increased crop yields in such soils.

Understanding the impact of elevated CO2 and O3 on growth and yield in Indian wheat cultivars: Implications for food security in a changing climate.

The pressing issue of increasing tropospheric ozone (O3) levels necessitates the development of effective stress management strategies for plant protection. While considerable research has elucidated the adverse impacts of O3, understanding the combined effects of O3 and CO2 requires further investigation. This study focuses on assessing the response of stomatal O3 flux under various O3 and CO2 treatments, individually and in combination, and their repercussions on physiological, growth, and yield attributes in two Indian wheat cultivars, HUW-55 and PBW-550, which exhibit varying levels of sensitivities against elevated O3. Results indicated significant alterations in stomatal O3 flux in both O3-sensitive and tolerant wheat cultivars across different treatments, influencing the overall yield outcomes. Particularly, the ECO2+EO3 treatment demonstrated more positive yield protection in the O3-sensitive cultivar PBW-550, compared to HUW-55 indicating enhanced allocation of photosynthates towards reproductive development in PBW-550, compared to the tolerant cultivar HUW-55, as evidenced by higher harvest index (HI). Furthermore, the study revealed a stronger correlation between yield response and stomatal O3 flux in PBW-550 (R2 = 0.88) compared to HUW-55 (R2 = 0.79), as indicated by a steeper regression slope for PBW-550. The research also confirmed the role of elevated CO2 in reducing stomatal O3- flux in the tested cultivars, with discernible effects on their respective yield responses. Further experimentation is necessary to confirm these results across different cultivars exhibiting varying sensitivities to O3. These findings can potentially revolutionize agricultural productivity in regions affected by O3 stress. The criteria for recommending cultivars for agricultural practices should not be based only on their sensitivity/tolerance to O3. Still, they should also consider the effect of CO2 fertilization in the growing area. This experiment offers hope to sustain global food security, as the O3-sensitive wheat cultivar also showed promising results at elevated CO2. In essence, this research could pave the way for more resilient agricultural systems in the era of changing climate under elevated O3 and CO2 conditions.

Integration of ultrasound and microwave pretreatments with solid-state fermentation enhances the release of sugars, organic acids, and phenolic compounds in wheat bran.

Wheat bran (WB), a byproduct of milling, is rich in bioactive compounds with significant health benefits. This study aimed to enhance the release of phenolic compounds, sugars, and organic acids from WB by integrating ultrasound (UsP) and microwave (MWP) pretreatments with solid-state fermentation (SSF). UsP and MWP disrupted WB cell walls, followed by SSF with Aspergillus niger. UsP increased total phenolic content by 21.30 % on day 1 of SSF. UsP and MWP boosted the availability of bound phenolic compounds like vanillic acid and dihydroxybenzoic acid. Both pretreatments enhanced antioxidant activity compared to untreated fermented WB, with peak activity on day 5 of fermentation at 1411 ± 5.156 µM Trolox/100 g DW for UsP WB and 291.6 ± 1.092 µM Trolox/100 g DW for MWP WB. This integrated approach improved the extraction efficiency of fermentable monosaccharides, particularly glucose and xylose, offering a sustainable bioprocessing strategy for WB valorization and supporting the circular bioeconomy.

Reducing nitrogen fertilizer usage coupled with organic substitution improves soil quality and boosts tea yield and quality in tea plantations.

BACKGROUND: The utilization of chemical fertilizers is a key measure for maintaining tea yield and quality, but excessive use has negative environmental impacts. The substitution of chemical fertilizer with organic fertilizer has been promoted to sustain crop yield and soil quality. However, knowledge gaps regarding the effects of organic substitution on soil quality and tea yield in tea plantations still exist. RESULTS: A field experiment was conducted to investigate the influence of organic substitution treatments (i.e. 25% partial substitution: biogas slurry + green manure + formula fertilizer, BFG; sheep manure + formula fertilizer, OFF; 100% complete substitution: sheep manure + green manure, OG) on the soil quality, tea yield and quality, and nitrogen utilization efficiency in southwestern China. Results showed that all organic substitution treatments slightly increased soil pH, and significantly increased soil organic matter by 13.22-14.88% compared to conventional fertilization (CF). The BFG treatment was the most effective in enhancing the soil quality index, showing increases of 16.80%, 8.37% and 24.87% higher than the CF, OFF and OG treatments, respectively. Tea yield significantly increased under the BFG, OFF and OG treatments by 11.97%, 13.58% and 5.90% compared to CF, respectively. The BFG treatment increased the amino acid content by 7.78% and decreased the tea polyphenol/amino acid ratio by 6.87%. Additionally, the BFG, OFF and OG treatments greatly increased the nitrogen utilization efficiency of young sprouts by 70.71%, 82.54% and 34.28%, respectively. CONCLUSION: Overall, partial organic substitution could effectively improve soil quality while maintaining tea yield. © 2024 Society of Chemical Industry.

Cascade Enhancement and Efficient Collection of Single Photon Emission under Topological Protection.

High emission rate, high collection efficiency, and immunity to defects are the requirements of implementing on-chip single photon sources. Here, we theoretically demonstrate that both cascade enhancement and high collection efficiency of emitted photons from a single emitter can be achieved simultaneously in a topological photonic crystal containing a resonant dielectric nanodisk. The nanodisk excited by a magnetic emitter can be regarded as a large equivalent magnetic dipole. The near-field overlapping between this equivalent magnetic dipole and edge state enables achieving a cascade enhancement of single-photon emission with a Purcell factor exceeding 4 × 103. These emitted photons are guided into edge states with a collection efficiency of more than 90%, which is also corresponding to quantum yield due to topological antiscattering and the absence of absorption. The proposed mechanism under topological protection has potential applications in on-chip light-matter interactions, quantum light sources, and nanolasers.