Establishment of a pMCAO model in SD rats and screening for behavioral indicators suitable for long-term monitoring.

OBJECTIVE: This study aimed to establish a permanent middle cerebral artery occlusion (pMCAO) model in rats to simulate the pathological process of stroke patients with no reperfusion. And screen highly sensitive items that could be used to detect long-term behavioral abilities in rat of intraluminal suture models. METHOD: Established the pMCAO model then tested the rats for the bilateral asymmetry, modified neurological severity score, grid-walking, cylinder, rotating, and water maze test from week 1 to week 16. RESULTS: The infarct volume of the model rats was stable (26.72% ±1.86%). The sensorimotor test of bilateral asymmetry, grid-walking, cylinder, and mNSS test showed significant differences from week 1 to week 16 after injury. The water maze test at week 16 showed significant differences in spatial exploration and learning ability between the two groups. We confirmed that there was no significant difference between MRI and TTC staining in detecting the degree of brain injury, which facilitated the diversity of subsequent detection methods. We also confirmed that at multiple time points, grid, cylinder and water maze test were significantly positively correlated with rat brain infarct volume. CONCLUSION: They are suitable for the long-term observation of behaviors in the sequela stage of stroke in rat.

Volumetric and Asymmetric Index Analysis of Subcortical Structures in Multiple Sclerosis Patients: A Retrospective Study Using volBrain Software.

Introduction Multiple sclerosis (MS) is a chronic and autoimmune disease that has a significant influence on the central nervous system, such as the brain and spinal cord, affecting millions of individuals globally. Understanding the connection between subcortical brain regions and MS is crucial for effective diagnostic and therapeutic approaches for treating this disabling disease. This study explores the relationship between volume and contours of asymmetry index of subcortical brain regions in individuals with MS using volBrain software (https://www.volbrain.net; developed by José V. Manjón (Valencia Polytechnic University, Valencia, Spain) and Pierrick Coupé (University of Bordeaux, Bordeaux, France)). Methods In our retrospective investigation, we admitted 100 Turkish individuals, comprising 50 patients diagnosed with relapsing-remitting MS (RRMS) (24 (48%) males and 26 (52%) females) and 50 healthy controls (23 (46%) males and 27 (54%) females), registered between October 2017 and February 2022 for five years and underwent assessment in the radiology department at the Teaching and Research Hospital of Kocaeli University; 1,150 Turkish patients were excluded from our study based on our exclusion criteria. We used magnetic resonance imaging with a 3-Tesla (3T) scanner and volBrain software to assess volumes (cm3) and asymmetry indexes due to asymmetry for different levels of atrophy of total intracranial, total brain, gray matter, white matter, and subcortical regions, the most affected regions in MS patients for both patient and control cohorts. Results Statistical analysis revealed a significant difference between patient and control groups (p < 0.001), with patient group mean age at 38.32 years and control group mean age at 32.88 years. Patient group exhibited lower values for total intracranial, total brain, gray matter, white matter, and cerebrospinal fluid volume compared to control group (p < 0.05). The results indicated a statistically significant decrease (p < 0.05) in the values for total intracranial and total brain volume, whereas all other values remained unchanged. We compared volumes of subcortical structures on the right and left sides and found that the putamen, thalamus, and globus pallidus had statistically lower values in the patient group than in the control group (p < 0.001), apart from the lateral ventricle. Furthermore, our retrospective investigation demonstrated a statistically significant difference in the globus pallidus asymmetry index, indicating a preference for the patient group (p < 0.05). A lower asymmetry index value signifies a larger volume for the right side of the subcortical regions of the brain when compared to the left side. Conclusion Brain atrophy, although characterized by irreversible tissue damage, is targeted by therapeutic interventions to prevent progression. It is, therefore, imperative to develop a universally accepted measurement standard for subcortical structures that also considers the inherent variability present within each structure. Our findings serve as an important basis and indicator for the determination of subcortical atrophy and asymmetry in MS, the prognosis of the disease, and the etiology of clinical symptoms. Subsequent research may benefit by adopting the novel approach of considering brain atrophy as an outcome rather than a predictor, thereby facilitating the elucidation of the intricate biological mechanisms that give rise to volume loss.

Risk Analysis of Breast Cancer by Using Bilateral Mammographic Density Differences: A Case-Control Study.

The identification of risk factors helps radiologists assess the risk of breast cancer. Quantitative factors such as age and mammographic density are established risk factors for breast cancer. Asymmetric breast findings are frequently encountered during diagnostic mammography. The asymmetric area may indicate a developing mass in the early stage, causing a difference in mammographic density between the left and right sides. Therefore, this paper aims to propose a quantitative parameter named bilateral mammographic density difference (BMDD) for the quantification of breast asymmetry and to verify BMDD as a risk factor for breast cancer. To quantitatively evaluate breast asymmetry, we developed a semi-automatic method to estimate mammographic densities and calculate BMDD as the absolute difference between the left and right mammographic densities. And then, a retrospective case-control study, covering the period from July 2006 to October 2014, was conducted to analyse breast cancer risk in association with BMDD. The study included 364 women diagnosed with breast cancer and 364 matched control patients. As a result, a significant difference in BMDD was found between cases and controls (P < 0.001) and the case-control study demonstrated that women with BMDD > 10% had a 2.4-fold higher risk of breast cancer (odds ratio, 2.4; 95% confidence interval, 1.3-4.5) than women with BMDD ≤ 10%. In addition, we also demonstrated the positive association between BMDD and breast cancer risk among the subgroups with different ages and the Breast Imaging Reporting and Data System (BI-RADS) mammographic density categories. This study demonstrated that BMDD could be a potential risk factor for breast cancer.

Bilateral asymmetry of quantitative parenchymal kinetics at ultrafast DCE-MRI predict response to neoadjuvant chemotherapy in patients with HER2+ breast cancer.

PURPOSE: To assess whether measurement of the bilateral asymmetry of semiquantitative and quantitative perfusion parameters from ultrafast dynamic contrast-enhanced MRI (DCE-MRI), allows early prediction of pathologic response after neoadjuvant chemotherapy (NAC) in patients with HER2+ breast cancer. MATERIALS AND METHODS: Twenty-eight female patients with HER2+ breast cancer treated with NAC who underwent pre-NAC ultrafast DCE-MRI (3-9 s/phase) were enrolled for this study. Four semiquantitative and two quantitative parenchymal parameters were calculated for each patient. Ipsilateral/contralateral (I/C) ratio (for four parameters) and the difference between (for two parameters) ipsi- and contra-lateral parenchymal kinetic parameters (kBPE) were compared for patients with pathologic complete response (pCR) and those having residual disease. Lasso regression with leave-one-out cross validation was used to determine the optimal combination of parameters for a regression model and multivariable logistic regression was used to identify independent predictors for pCR. Chi-squared test, two-sided t-test and Kruskal-Wallis test were used. RESULTS: The Ktrans I/C ratio cutoff value of 1.11 had a sensitivity of 83.3% and specificity of 75% for pCR. The ve I/C ratio cutoff value of 1.1 had a sensitivity of 75% and specificity of 81.3% for pCR. The area under the receiver operating characteristic curve of the three-kBPE parameter model, including initial area under the enhancement curve (AUC30) I/C ratio, KtransI/C ratio and ve I/C ratio, was 0.89 with sensitivity of 91.7% at specificity of 81.3%. CONCLUSION: Quantitative assessment of bilateral asymmetry kBPE from pre-NAC ultrafast DCE-MRI can predict pCR in patients with HER2+ breast cancer.

Horizontal jump asymmetries are associated with reduced range of motion and vertical jump performance in female soccer players.

BACKGROUND: Performance in jumping and change of direction tests are good proxies to reflect the skill level during soccer-specific actions. Greater inter-leg asymmetries have been identified as a risk factor for developing acute and overuse injuries and jeopardizing soccer performance. The aim of this study was to assess the association between asymmetry in the unilateral vertical and horizontal jump tests, ankle range of motion, linear velocity, and change of direction in a sample of highly trained adult female soccer players. METHODS: Thirty-eight highly trained female soccer players underwent a testing protocol including ankle dorsiflexion, single leg jumps for height (CMJ), distance (HJ), 40 m sprint and 180° change of direction tests. RESULTS: Within-session reliability was acceptable (CV ≤ 7.9%), and relative reliability showed good to excellent (ICC: 0.83 to 0.99). The one-way ANOVA reported higher inter-limb differences for change of direction deficit (10.9 ± 8.04%) and single leg CMJ (5.70 ± 5.22%). Pearson correlations highlighted significant relationships between horizontal jump asymmetries and ankle dorsiflexion (r = -0.41), CMJ (r = -0.36 to -0.49) and HJ (r = -0.28 to -0.56). CONCLUSIONS: Assessing inter-limb asymmetries through different methods can help scientists understand the specificity of their detrimental effects on soccer performance. Practitioners should be aware of these specificities as well as the magnitude and direction of the asymmetries when aiming to improve specific on-field skills.

Radiographic Assessment of Bilateral Asymmetry in the Upper Extremities of Living Humans.

Objective Injuries resulting from trauma or tumor resection may cause length alterations in the bones of the upper extremities (UE) requiring reconstruction. Direct contralateral bone is often used to determine the appropriate length for reconstruction but fails to account for potential asymmetry. Given the paucity of data assessing asymmetry in living populations and the need for accurate length approximation, we developed a study evaluating UE long bone asymmetry using radiographic imaging in living subjects. Methods Bilateral X-ray images previously obtained for traumatic injury or chronic osseous conditions were retrospectively collected for adult subjects (ages 18-81). After screening, 61 patients met the inclusion criteria: 28 radii, 29 ulnae, and 19 humeri. Three serial measurements were taken, and the median was used for subsequent analysis. Wilcoxon signed-rank tests were performed to assess differences in contralateral bone lengths. Bootstrapping was utilized to obtain sample sizes of 200, 500, and 1000 for each bone. Results The difference in mean absolute length was 27.0 mm for the humerus, 8.6 mm for the radius, and 7.5 mm for the ulna. Neither the left side nor the right side was significantly longer for any bone. In 57.9% (11/19) of patients, the right humerus was longer than the left; in 60.7% (17/28), the right radius was longer than the left; and in 48.3% (14/29), the right ulna was longer than the left. All other measurements showed the left was longer than the right. Wilcoxon signed-rank tests did not find significant differences between contralateral pairs in any direct measurement group. In bootstrap samples, significant differences in length (p ≤ 0.05) were seen in all samples (n = 200, 500, and 1000) for both humerus and radius but only the 1000 sample group for the ulna. Conclusions Direct contralateral measure may be an appropriate method of length estimation for the humerus, radius, and ulna in post-industrial humans.

Underwater Surface Electromyography for the Evaluation of Muscle Activity during Front Crawl Swimming: A Systematic Review.

This systematic review is aimed to provide an up-to-date summary and review on the use of surface electromyography (sEMG) in evaluating front crawl (FC) swim performance. Several online databases were searched by different combinations of selected keywords, in total 1956 articles were retrieved, and each article was assessed by a 10-item quality checklist. 16 articles were eligible to be included in this study, and most of the articles were evaluating the muscle activity about the swimming phases and focused on assessing the upper limbs muscles, only few studies have assessed the performance in starts and turns phases. Insufficient information about these two phases despite the critical contribution on final swimming time. Also, with the contribution roles of legs and trunk muscles in swimming performance, more research should be conducted to explore the overall muscle activation pattern and their roles on swimming performance. Moreover, more detailed description in participants' characteristics and more investigations of bilateral muscle activity and the asymmetrical effects on relevant biomechanical performance are recommended. Lastly, with increasing attention about the effects of muscles co-activation on swimming performance, more in-depth investigations on this topic are also highly recommended, for evaluating its influence on swimmers.

Using trunk kinematics to predict kinetic asymmetries during double-leg jump-landings in collegiate athletes following anterior cruciate ligament reconstruction.

BACKGROUND: Bilateral vertical ground reaction force (VGRF) and knee extension moment asymmetries are commonly observed during jumping and landing tasks following anterior cruciate ligament (ACL) reconstructions (ACLR) in collegiate athletes. Kinetic asymmetries during landings are associated with increased ACL re-injury risk. Efforts have been made to predict bilateral kinetic asymmetries using trunk kinematics during squats but not during jump-landings. RESEARCH QUESTION: To determine the correlations between trunk kinematics (medial-lateral shoulder positions, medial-lateral hip positions, and lateral trunk bending angles) and bilateral kinetic asymmetries (VGRF and knee extension moments) during double-leg jump-landings in collegiate athletes following ACLR. METHODS: Fifteen National Collegiate Athletic Association Division I athletes who had ACLR in the past 24 months participated. Eleven of them performed two assessments over the study period for a total of 26 assessments for data analyses. Athletes performed three double-leg countermovement jumps. Kinematics and kinetics data were collected. Medial-lateral shoulder and hip positions relative to ankle positions, lateral trunk bending angles, and kinetic asymmetries were calculated during the jumping (the lowest hip position until takeoff) and landing (the first 100 ms after initial contact) phases. RESULTS: Medial-lateral shoulder positions correlated with VGRF (r = 0.63, p < 0.001) and knee moment asymmetries (r = 0.53, p = 0.006) in the jumping phase. Medial-lateral hip positions correlated with VGRF (r = 0.61, p < 0.001; r = 0.52, p = 0.006) and knee moment asymmetries (r = 0.55, p = 0.004; r = 0.61, p < 0.001) in both jumping and landing phases. SIGNIFICANCE: Medial-lateral hip positions correlated with kinetic asymmetries during double-leg jump-landings in collegiate athletes following ACLR. A 2D assessment using a standard video camera might be used as a low-cost and clinically applicable tool to assess bilateral kinetic asymmetries by quantifying medial-lateral hip positions during jump-landings following ACLR.

Bilateral asymmetry of otoliths from Collichthys lucidus of different sizes in Haizhou Bay and Xiangshan Bay.

In recent years, the frequent occurrence of most human activities has seriously affected the structure and functioning of coastal ecosystems. The asymmetric relationship between the left and right otoliths of fish is often used to test the difference in fluctuating asymmetry (FA) reflected by the square of the coefficient of asymmetric variation (CV2 a ), which can be regarded as an important step in the study of marine environmental pressure and implementation of offshore ecological restoration. In this study, the authors tested the bilateral FA of Collichthys lucidus in the coastal waters of Haizhou Bay, Jiangsu, and Xiangshan Bay, Zhejiang, China, using four sagittal otolith characters (length, width, perimeter and area) as biological characters. The results showed that the value of CV2 a in otolith width (more sensitive to environmental pressure) of C. lucidus in Xiangshan Bay was higher than that in Haizhou Bay, indicating that the environmental pressure on Xiangshan Bay was relatively high. The authors did not find any significant differences in the FA of otoliths between different body sizes of C. lucidus, which may be related to the short-distance migration in different regions and the dietary shifts in the life history of this species. The results have conservation and management implications for this population.

Differential Repeated Sprinting Training in Youth Basketball Players: An Analysis of Effects According to Maturity Status.

The differential learning approach, which includes fluctuations that occur without movement repetitions and without corrections has received growing interest in the skill acquisition field. This study aimed to determine the effects of a 9-week training intervention involving differential repeated sprint training on a series of physical tests in youth basketball players. A total of 29 participants with different maturity statuses (pre-peak height velocity (PHV), n = 7; mid-PHV, n = 6; post-PHV, n = 16) completed 2 sessions per week of differential repeated sprint training for a period of 9 weeks. Sessions consisted of 2 × 10 repetitions sprints of 20-m whereby participants were instructed to perform various additional fluctuations for each repetition. Before and after the training intervention, participants completed jumping tests (countermovement jump (CMJ), single-leg CMJs, the modified 505 agility test, and straight sprinting tests (0-10 splits time), and maturity status was evaluated as well. Within-group analysis showed improvement in CMJ asymmetries and changes in direction asymmetries and 10-m sprint performance for the pre-, mid-, and post-PHV groups, respectively (p < 0.05), with large to very large effects. Analysis of covariance demonstrated that changes in sprint time in post-PHV players were greater than in the pre- and mid-PHV groups (p < 0.05), with moderate effect. Adding random fluctuations during repeated sprint training appear to be a suitable and feasible training strategy for maintaining and enhancing physical performance in youth basketball players, irrespective of maturity status. Furthermore, the present findings encourage practitioners to implement the present approach in youth athletes to improve their physical performance, but they should be aware that training response can vary according to maturity status.

Anterior-Posterior and Inter-Limb Lower Body Strength Asymmetry in Soccer, Basketball, Futsal, and Volleyball Players.

Background and Objectives: The purpose of this study was twofold: (1) to examine strength asymmetries in elite athletes of different sports and (2) to examine the magnitude of asymmetries between elite and u18 athletes. Materials and Methods: A total of 254 athletes participated in this study. For the first purpose of the study, the group consisted of adult professional male basketball players (n = 30), elite male (n = 30) and female soccer players (n = 20), male futsal players (n = 30), elite male goalkeepers (n = 22), and professional female volleyball players (n = 20). For the second purpose, male youth goalkeepers (n = 22), youth female (n = 20) and male soccer players (n = 30), and youth male basketball players (n = 30) were compared to the adult athletes of the same sport. Asymmetries were measured utilizing a Humac Norm and Rehabilitation device. Testing included three maximal concentric flexion and extension repetitions at an angular speed of 60°/s. The differences in asymmetries were assessed using ANOVA followed by an LS means post-hoc analysis. An independent samples t-test was used to identify the differences between adult and youth players. Results: It was indicated that none of the groups demonstrated asymmetries greater than 10%, other than the elite female soccer players and female volleyball players. Significant differences were demonstrated between youth and adult soccer players (males and females), with the adult groups demonstrating increased asymmetries. Conclusions: Special consideration should be given to female soccer players and volleyball players, as soccer and volleyball practice and competition at the professional level may induce greater lower -limb asymmetries. The isokinetic parameters can be useful for planning strength and conditioning interventions in order to reduce or prevent those imbalances. Additional unilateral and bilateral jumping testing is encouraged for the verification of imbalances.

Differences Between Ipsilateral and Contralateral Early Parenchymal Enhancement Kinetics Predict Response of Breast Cancer to Neoadjuvant Therapy.

RATIONALE AND OBJECTIVES: To determine whether kinetics measured with ultrafast dynamic contrast-enhanced magnetic resonance imaging in tumor and normal parenchyma pre- and post-neoadjuvant therapy (NAT) can predict the response of breast cancer to NAT. MATERIALS AND METHODS: Twenty-four patients with histologically confirmed invasive breast cancer were enrolled. They were scanned with ultrafast dynamic contrast-enhanced magnetic resonance imaging (3-7 seconds/frame) pre- and post-NAT. Four kinetic parameters were calculated in the segmented tumors, and ipsi- and contra-lateral normal parenchyma: (1) tumor (tSE30) or background parenchymal relative enhancement at 30 seconds (BPE30), (2) maximum relative enhancement slope (MaxSlope), (3) bolus arrival time (BAT), and (4) area under relative signal enhancement curve for the initial 30 seconds (AUC30). The tumor kinetics and the differences between ipsi- and contra-lateral parenchymal kinetics were compared for patients achieving pathologic complete response (pCR) vs those who had residual disease after NAT. The chi-squared test and two-sided t-test were used for baseline demographics. The Wilcoxon rank sum test and one-way analysis of variance were used for differential responses to therapy. RESULTS: Patients with similar pre-NAT mean BPE30, median BAT and mean AUC30 in the ipsi- and contralateral normal parenchyma were more likely to achieve pCR following NAT (p < 0.02). Patients classified as having residual cancer burden (RCB) II after NAT showed higher post-NAT tSE30 and tumor AUC30 and higher post-NAT MaxSlope in ipsilateral normal parenchyma compared to those classified as RCB I or pCR (p < 0.05). CONCLUSION: Bilateral asymmetry in normal parenchyma could predict treatment outcome prior to NAT. Post-NAT tumor kinetics could evaluate the aggressiveness of residual tumor.

Prediction of Short-Term Breast Cancer Risk with Fusion of CC- and MLO-Based Risk Models in Four-View Mammograms.

This study performed and assessed a novel program to improve the accuracy of short-term breast cancer risk prediction by using information from craniocaudal (CC) and mediolateral-oblique (MLO) views of two breasts. An age-matched dataset of 556 patients with at least two sequential full-field digital mammography examinations was applied. In the second examination, 278 cases were diagnosed and pathologically verified as cancer, and 278 were negative, while all cases in the first examination were negative (not recalled). Two generalized linear-model-based risk prediction models were established with global- and local-based bilateral asymmetry features for CC and MLO views first. Then, a new fusion risk model was developed by fusing prediction results of the CC- and MLO-based risk models with an adaptive alpha-integration-based fusion method. The AUC of the fusion risk model was 0.72 ± 0.02, which was significantly higher than the AUC of CC- or MLO-based risk model (P < 0.05). The maximum odds ratio for CC- and MLO-based risk models were 8.09 and 5.25, respectively, and increased to 11.99 for the fusion risk model. For subgroups of patients aged 37-49 years, 50-65 years, and 66-87 years, the AUCs of 0.73, 0.71, and 0.75 for the fusion risk model were higher than AUC for CC- and MLO-based risk models. For the BIRADS 2 and 3 subgroups, the AUC values were 0.72 and 0.71 respectively for the fusion risk model which were higher than the AUC for the CC- and MLO-based risk models. This study demonstrated that the fusion risk model we established could effectively derive and integrate supplementary and useful information extracted from both CC and MLO view images and adaptively fuse them to increase the predictive power of the short-term breast cancer risk assessment model.

Comparative reconstruction of the predatory feeding structures of the polyphenic nematode Pristionchus pacificus.

Pristionchus pacificus is a nematode model for the developmental genetics of morphological polyphenism, especially at the level of individual cells. Morphological polyphenism in this species includes an evolutionary novelty, moveable teeth, which have enabled predatory feeding in this species and others in its family (Diplogastridae). From transmission electron micrographs of serial thin sections through an adult hermaphrodite of P. pacificus, we three-dimensionally reconstructed all epithelial and myoepithelial cells and syncytia, corresponding to 74 nuclei, of its face, mouth, and pharynx. We found that the epithelia that produce the predatory morphology of P. pacificus are identical to Caenorhabditis elegans in the number of cell classes and nuclei. However, differences in cell form, spatial relationships, and nucleus position correlate with gross morphological differences from C. elegans and outgroups. Moreover, we identified fine-structural features, especially in the anteriormost pharyngeal muscles, that underlie the conspicuous, left-right asymmetry that characterizes the P. pacificus feeding apparatus. Our reconstruction provides an anatomical map for studying the genetics of polyphenism, feeding behavior, and the development of novel form in a satellite model to C. elegans.

The Effect of Differential Repeated Sprint Training on Physical Performance in Female Basketball Players: A Pilot Study.

This pilot study aimed to determine the effects of differential learning in sprint running with and without changes of direction (COD) on physical performance parameters in female basketball players and to determine the feasibility of the training protocol. Nine female basketball players completed 4 weeks of repeated sprint training (RST) with (COD, n = 4) or without (NCOD, n = 5) changes of direction. A battery of sprints (0-10 and 0-25 m), vertical jumps (counter movement jump (CMJ), drop jump, and single-leg CMJs), and COD tests were conducted before and after intervention. NCOD completed two sets of ten sprints of 20 m, whereas COD performed 20 m sprints with a 180 degree turn at 10 m, returning to the starting line. Before each sprint, participants were instructed to provide different fluctuations (i.e., differential learning) in terms of varying the sprint. Both groups had 30 s of passive recovery between two sprints and 3 min between sets. A significant effect of time for the 0-10 m sprint, CMJ, and single leg-CMJ asymmetries were observed. Adding "erroneous" fluctuation during RST seems to be a suitable and feasible strategy for coaches to enhance physical performance in young female basketball players. However, further studies including larger samples and controlled designs are recommended to strengthen present findings.

QUANTIFYING BILATERAL INFECTION PATTERNS IN THE TREMATODE ALLOGLOSSIDIUM RENALE.

Within-host distributions of parasites can have relevance to parasite competition, parasite mating, transmission, and host health. We examined the within-host distribution of the adult trematode Alloglossidium renale infecting the paired antennal glands of grass shrimp. There are 4 possible parasite distributions for infections of paired organs: random, uniform, biased aggregation to 1 particular organ (e.g., left vs. right), or inconsistently biased (aggregated, but does not favor 1 side). Previous work has shown that morphological asymmetries in hosts can lead to biased infections of paired organs. Apparent symmetry between the antennal glands of grass shrimp leads to the prediction that there would be no bias for 1 particular organ. However, an alternative prediction stems from the fact that A. renale is hermaphroditic: aggregation between glands would increase outcrossing opportunities and thus, avoid inbreeding via self-mating. Existing methods to test for an overall pattern did not apply to the A. renale system because of low-intensity infections as well as many 0 values for abundance per unit of the antennal gland. Hence, we used Monte Carlo simulations to determine if the observed overall patterns differed from those expected by randomly allocating parasites into groups of 2. We found that in 3 of 4 data sets, A. renale infections did not deviate from random distributions. The fourth data set had a more uniform pattern than expected by chance. As there was no aggregation between glands and the proportion of worms in single gland infections did not differ from that expected by chance alone, we found no evidence of inbreeding avoidance as might be manifested via a within-host distribution. Given the large proportion of worms in single infections, we predict as a major evolutionary outcome that populations of A. renale will be largely inbred.

Extraction of metastasis hotspots in a whole-body bone scintigram based on bilateral asymmetry.

PURPOSE: A hotspot of bone metastatic lesion in a whole-body bone scintigram is often observed as left-right asymmetry. The purpose of this study is to present a network to evaluate bilateral difference of a whole-body bone scintigram, and to subsequently integrate it with our previous network that extracts the hotspot from a pair of anterior and posterior images. METHODS: Input of the proposed network is a pair of scintigrams that are the original one and the flipped version with respect to body axis. The paired scintigrams are processed by a butterfly-type network (BtrflyNet). Subsequently, the output of the network is combined with the output of another BtrflyNet for a pair of anterior and posterior scintigrams by employing a convolutional layer optimized using training images. RESULTS: We evaluated the performance of the combined networks, which comprised two BtrflyNets followed by a convolutional layer for integration, in terms of accuracy of hotspot extraction using 1330 bone scintigrams of 665 patients with prostate cancer. A threefold cross-validation experiment showed that the number of false positive regions was reduced from 4.30 to 2.13 for anterior and 4.71 to 2.62 for posterior scintigrams on average compared with our previous model. CONCLUSIONS: This study presented a network for hotspot extraction of bone metastatic lesion that evaluates bilateral difference of a whole-body bone scintigram. When combining the network with the previous network that extracts the hotspot from a pair of anterior and posterior scintigrams, the false positives were reduced by nearly half compared to our previous model.

Bilateral asymmetry in the gait deviation index in school-aged children with the trait of developmental coordination disorder.

BACKGROUND: Although previous studies have identified asymmetry in gait parameters in children with developmental coordination disorder (DCD), they have not investigated whether these findings coexist with asymmetry in gait quality, as represented by the gait deviation index (GDI). RESEARCH QUESTION: This study sought to measure gait symmetry in children with traits of DCD (DCD trait) to elucidate the characteristic gait pattern. METHODS: This study included 172 (82 girls, 90 boys) participants with and without DCD trait (age: 6-12 years), as assessed using the Japanese version of the Developmental Coordination Disorder Questionnaire (DCDQ-J), which consists of three subscales. Three-dimensional gait analysis data were obtained during the gait trials. GDI, step length, and step time data were recorded for both the right and left legs, and symmetry ratios were calculated. RESULTS: Participants in the DCD trait group (n = 30) had a significantly lower GDI score (p < 0.0001) and a higher GDI symmetry ratio (p = 0.004) than typically developing children. Furthermore, DCD trait was related to the GDI symmetry ratio (odds ratio, 1.2; p = 0.001; 95 % confidence interval, 1.062-1.289). The control during movement, which was a DCDQ-J subscale, was negatively correlated with the GDI symmetry ratio (r=-0.257, p<0.001). SIGNIFICANCE: This study's results lend support to the hypothesis that an increase in the GDI symmetry ratio is associated with the DCD trait. A quantitative assessment of gait performance is important to help identify gait asymmetry, including gait quality, in children with a DCD trait. Children with a DCD trait have a poor ability to control the interaction of lower leg movements, which may cause bilateral asymmetry in gait quality.

Diferente asimetría craneal según sexo en el conejo toy / Different skull asymmetry according to sex in toy rabbits

RESUMEN: Los conejos denominados toy son seleccionados por su apariencia pedomórfica ('infantilizada'). El objetivo principal de este estudio fue analizar las asimetrías craneales en este tipo de conejo. Para ello, se estudiaron un total de 46 cráneos adultos (9 machos y 37 hembras), recurriéndose a técnicas de morfométrica geométrica a partir 10 hitos anatómicos pareados y 3 en el plano sagital, en la cara dorsal del cráneo. Nuestra hipótesis es que a través de la selección artificial en los machos (objetivo principal para la obtención de nuevos fenotipos) las asimetrías se expresarán como valores de asimetría más altos. El tamaño de los cráneos resultó similar entre ambos sexos, pero presentaban la asimetría fluctuante fue mayor en machos. La asimetría fluctuante se considera un indicador negativo de la capacidad de resistir pequeñas anomalías en su desarrollo, anomalías que son generalmente el resultado de estrés genético o ambiental. En otras palabras, los machos estarían sujetos a una selección más fuerte y, en consecuencia, se verían más afectados por el manejo, en comparación con las hembras. Estos resultados pueden facilitar la comprensión de los patrones y procesos subyacentes al dimorfismo sexual en fenotipos extremos, como lo son los de los conejos toy.

SUMMARY: Toy rabbits are selected for their paedomorphic ('babyish') appearance. The main objective of this study is to analyse the skull asymmetries of toy rabbits. For this purpose, a total of 46 adult skulls (9 males and 37 females) were studied. Geometric morphometric technique with five pairs of anatomical landmarks and three on sagittal plane on dorsal aspect of skull was used. Our hypothesis is that through artificial selection on males (which are the main subject for obtaining new phenotypes among toys) asymmetries will be expressed as higher asymmetrical values. Skulls' size was similar between sexes, but males presented a higher level of fluctuating asymmetry. Fluctuating asymmetry indicates a negative ability to buffer subtle developmental anomalies, normally from genetic or environmental origin. In other words, males would be under stronger directed selection and consequently be more affected by management (including selective) factors compared to females. These results can allow a better understanding of the patterns and processes underlying sexual shape dimorphism, especially with extreme phenotypes, as toy rabbits are.

Fluctuating asymmetry in the size of the otolith of Engraulis australis (Shaw, 1790) recovered from the food of the Australasian gannet, Morus serrator, Hauraki Gulf, New Zealand.

The fluctuating asymmetry values of the two otolith parameters: length and width of Engraulis australis, recovered from the food of gannet, which inhabit colonies in islands at Hauraki Gulf, New Zealand was calculated in this study. The dissimilarity value of the otolith width was higher than that of the otolith length. An inclination of upsurge in the dissimilarity values with the fish length was noted for the otolith sizes studied. The dissimilarity value in the two otolith sizes was the lowest for fish having total length of 115-154 mm, and the highest for fish with length of 151-160 mm. The conceivable reason of the dissimilarity obtained could be related to diverse contaminants and their presence in the study location.