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1.
J Fungi (Basel) ; 10(7)2024 Jun 27.
Article in English | MEDLINE | ID: mdl-39057336

ABSTRACT

The use of arbuscular mycorrhizal fungi (AMF) offers promising benefits to agriculture in the Amazon regions, where soils are characteristically acidic and nutrient-poor. The purpose of this research was to investigate the potential effects of two recently described species of AMF (Nanoglomus plukenetiae and Rhizoglomus variabile) native to the Peruvian Amazon for improving the plant growth of Plukenetia volubilis (inka nut or sacha inchi) and protecting the roots against soil pathogens. Two assays were simultaneously conducted under greenhouse conditions in Peru. The first focused on evaluating the biofertilizer effect of AMF inoculation, while the second examined the bioprotective effect against the root knot nematode, Meloidogyne incognita. Overall, the results showed that AMF inoculation of P. volubilis seedlings positively improved their development, particularly their biomass, height, and the leaf nutrient contents. When seedlings were exposed to M. incognita, plant growth was also noticeably higher for AMF-inoculated plants than those without AMF inoculation. Nematode reproduction was significantly suppressed by the presence of AMF, in particular R. variabile, and especially when inoculated prior to nematode exposure. The dual AMF inoculation did not necessarily lead to improved crop growth but notably improved P and K leaf contents. The findings provide strong justification for the development of products based on AMF as agro-inputs to catalyze nutrient use and uptake and protect crops against pests and diseases, especially those that are locally adapted to local crops and cropping conditions.

2.
BMC Plant Biol ; 24(1): 642, 2024 Jul 08.
Article in English | MEDLINE | ID: mdl-38972980

ABSTRACT

Among the several threats to humanity by anthropogenic activities, contamination of the environment by heavy metals is of great concern. Upon entry into the food chain, these metals cause serious hazards to plants and other organisms including humans. Use of microbes for bioremediation of the soil and stress mitigation in plants are among the preferred strategies to provide an efficient, cost-effective, eco-friendly solution of the problem. The current investigation is an attempt in this direction where fungal strain PH1 was isolated from the rhizosphere of Parthenium hysterophorus which was identified as Aspergillus niger by sequence homology of the ITS 1 and ITS 4 regions of the rRNA. The strain was tested for its effect on growth and biochemical parameters as reflection of its potential to mitigate Pb stress in Zea mays exposed to 100, 200 and 500 µg of Pb/g of soil. In the initial screening, it was revealed that the strain has the ability to tolerate lead stress, solubilize insoluble phosphate and produce plant growth promoting hormones (IAA and SA) and other metabolites like phenolics, flavonoids, sugar, protein and lipids. Under 500 µg of Pb/g of soil, Z. mays exhibited significant growth retardation with a reduction of 31% in root length, 30.5% in shoot length, 57.5% in fresh weight and 45.2% in dry weight as compared to control plants. Inoculation of A. niger to Pb treated plants not only restored root and shoot length, rather promoted it to a level significantly higher than the control plants. Association of the strain modulated the physio-hormonal attributes of maize plants that resulted in their better growth which indicated a state of low stress. Additionally, the strain boosted the antioxidant defence system of the maize there by causing a significant reduction in the ascorbic acid peroxidase (1.5%), catalase (19%) and 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical scavenging activity (33.3%), indicating a lower stress condition as compared to their non-inoculated stressed plants. Based on current evidence, this strain can potentially be used as a biofertilizer for Pb-contaminated sites where it will improve overall plant health with the hope of achieving better biological and agricultural yields.


Subject(s)
Antioxidants , Aspergillus niger , Lead , Phosphates , Photosynthesis , Zea mays , Zea mays/growth & development , Zea mays/microbiology , Zea mays/drug effects , Zea mays/metabolism , Aspergillus niger/metabolism , Lead/metabolism , Antioxidants/metabolism , Photosynthesis/drug effects , Phosphates/metabolism , Soil Pollutants/metabolism , Stress, Physiological , Biodegradation, Environmental
3.
Plants (Basel) ; 13(13)2024 Jul 03.
Article in English | MEDLINE | ID: mdl-38999674

ABSTRACT

Phosphorus (P) is an important and irreplaceable macronutrient. It is central to energy and information storage and exchange in living cells. P is an element with a "broken geochemical cycle" since it lacks abundant volatile compounds capable of closing the P cycle. P fertilizers are critical for global food security, but the reserves of minable P are scarce and non-evenly distributed between countries of the world. Accordingly, the risks of global crisis due to limited access to P reserves are expected to be graver than those entailed by competition for fossil hydrocarbons. Paradoxically, despite the scarcity and value of P reserves, its usage is extremely inefficient: the current waste rate reaches 80% giving rise to a plethora of unwanted consequences such as eutrophication leading to harmful algal blooms. Microalgal biotechnology is a promising solution to tackle this challenge. The proposed review briefly presents the relevant aspects of microalgal P metabolism such as cell P reserve composition and turnover, and the regulation of P uptake kinetics for maximization of P uptake efficiency with a focus on novel knowledge. The multifaceted role of polyPhosphates, the largest cell depot for P, is discussed with emphasis on the P toxicity mediated by short-chain polyPhosphates. Opportunities and hurdles of P bioremoval via P uptake from waste streams with microalgal cultures, either suspended or immobilized, are discussed. Possible avenues of P-rich microalgal biomass such as biofertilizer production or extraction of valuable polyPhosphates and other bioproducts are considered. The review concludes with a comprehensive assessment of the current potential of microalgal biotechnology for ensuring the sustainable usage of phosphorus.

4.
J Environ Manage ; 366: 121796, 2024 Jul 14.
Article in English | MEDLINE | ID: mdl-39008925

ABSTRACT

Common wastewater treatment strategies in the food industry do not include efficient remediation strategies for nitrogen, phosphorous and organic carbon. Incorporating microalgae in water treatment plants is rising in popularity because of their high nutrient and trace element uptake driven by light. In this study, four different side streams from an Austrian potato processing company have been screened for their applicability of microalgal cultivation. The side streams were assessed for Chlorella vulgaris growth and their requirement of any additional pretreatment or media supplementation. One side stream specifically, called blanching water II, a stream generated by boiling the potatoes for ease of peeling, turned out very useful to cultivate Chlorella vulgaris and concomitantly remedy the wastewater. Compared to a state-of-the-art cultivation in BG11, cultivating Chlorella vulgaris in blanching water II led to a 45 % increase in specific growth rate of 1.29 day-1 and a 48% increase in biomass productivity to 294.6 mg/L/day, while all nitrogen and phosphate present in the side stream were metabolized. Overall, the results demonstrate that the water remediation process for blanching water II shows vast potential in regard to water purification and waste to value approaches.

5.
Plants (Basel) ; 13(14)2024 Jul 11.
Article in English | MEDLINE | ID: mdl-39065437

ABSTRACT

Currently, different strategies, including the application of bio-fertilizers, are used to ameliorate the adverse effects posed by salinity stress as the major global problem in plants. Fish waste is suggested as a novel bio-fertilizer to mitigate the effects of biotic and abiotic stresses. In this investigation, an experiment was conducted to investigate the effects by applying different concentrations (0, 5, 10, and 15% (v/v)) of fish waste bio-fertilizer on stevia plants grown under salt stress conditions (0, 20, 40, and 60 mM of NaCl). Results showed that salinity negatively affected growth parameters, the photosynthetic pigments, the relative water content, and the chlorophyll fluorescence parameters while increased the activity of antioxidant enzymes, total phenol, hydrogen peroxide (H2O2), malondialdehyde (MDA), proline, and total carbohydrates compared with control samples. On the other hand, the application of fish waste bio-fertilizer mitigated the effects of salinity stress by enhancing growth and mitigating stress-relative markers, especially at the highest salinity level (60 mM). Overall, fish waste bio-fertilizer could be considered a sustainable, innovative approach for the alleviation of salinity stress effects in plants and, in addition, fish waste bio-fertilizer did not cause more salinity issues, at least with the applied doses and experiment time, which is an imperative aspect.

6.
Bioresour Technol ; 408: 131165, 2024 Jul 26.
Article in English | MEDLINE | ID: mdl-39069142

ABSTRACT

This study explores the enhancement of phosphate rock (PR) solubilization through solid-state fermentation (SSF) by optimizing oxalic acid production using Aspergillus niger. Key process parameters, including the use of agro-industrial by-products (sugarcane bagasse (SCB), wheat bran (WB), soybean bran (SB)), pH levels, sucrose supplementation, and methanol addition, were systematically evaluated through sequential experimental designs. The results identified SCB and SB in a 1:1 ratio as the most effective substrate. Remarkably, the inclusion of methanol (7 %) and sucrose (0.5 %) resulted in a 3-fold increase in oxalic acid production. Under these optimized conditions, significant phosphorus solubilization of Bayóvar, Itafós, and Registro PRs was achieved, with Bayóvar rock releasing up to 12.1 g/kgds of soluble P (63.8 % efficiency). Additionally, the SSF process effectively released organic phosphorus from the agro-industrial substrates. These findings hold promise for advancing the bio-based economy and developing future industrial biofertilizers.

7.
Bioresour Technol ; 408: 131163, 2024 Jul 28.
Article in English | MEDLINE | ID: mdl-39079573

ABSTRACT

Microalgal biomass is gaining increasing attention to produce high-value co-products. This study proposes integrating Chlorella microalgal biomass into a zero-waste biorefining system, aiming to produce biodiesel and biofertilizer. It investigates optimal conditions for ultrasound-assisted deep eutectic solvent (DES) pretreatment and lipid recovery to enhance the extraction of lipids. Optimal DES pretreatment was identified as a 1.6:1 acetic acid-to-choline chloride molar ratio, 0.36 g biomass loading, and 2.50 min of pretreatment. Lipid recovery succeeded with a 10-minute extraction time and a 1:3 methanol-to-butanol volume ratio. These conditions yielded biodiesel-quality lipids at 139.52 mg/g microalgal biomass with superior fuel characteristics. The de-oiled microalgal biomass residue exhibited promise as a lettuce biofertilizer, enhancing photosynthetic pigments but potentially reducing yields by 40 %. The study also notes changes in rhizosphere microbial communities, indicating both stimulatory and inhibitory effects on beneficial microbes. This study has the potential to enhance sustainability in energy, agriculture, and the environment.

8.
Microbiol Res ; 287: 127855, 2024 Jul 25.
Article in English | MEDLINE | ID: mdl-39079269

ABSTRACT

Potato is an important crop due to its high contents of starch, protein, and various vitamins and minerals. Biofertilizers are composed of plant growth promoting microbes (PGPMs) which are essential for improving the growth and resistance of potato. However, little information has focused on the modes of inoculation of biofertilizers on plant growth and microecology. This study aims to reveal the response mechanism of the potato to three modes of inoculation of biofertilizers all containing PGPM Bacillus amyloliquefaciens EZ99, i.e. scattered mode of 5 kg/ha biofertilizer (M5), soaking seed tubers with dissolved 5 kg/ha biofertilizer (MZG), and scattered mode of 3 kg/ha biofertilizer + 2 kg/ha sucrose (MY34) in alkaline loess field through multi-omics analysis of transcriptome, metabolome and microbiome. The physiological result revealed that two application modes of equal amount of biofertilizer M5 and MZG significantly improved the growth and yield of potatoes. Furthermore, the transcriptome of potato exhibited sets of differentially expressed genes enriched in photosynthesis, sugar metabolism, and phenylpropanoid biosynthesis among the three modes, with the M5 mode exhibiting overall up-regulation of 828 genes. Based on the untargeted metabolomic analysis of potato tuber, M5 mode significantly accumulated sucrose, while MZG and MY34 mode significantly accumulated the stress metabolites euchrenone b6 and mannobiose, respectively. Besides, the microbial structure of potato rhizosphere showed that the diversity of bacteria and fungi was similar in all soils, but their abundances varied significantly. Specifically, beneficial Penicillium was enriched in M5 and MZG soils, whereas MY34 soil accumulated potential pathogens Plectosphaerella and saccharophilic Mortierella. Collectively, these e findings highlight that MZG is the most effective mode to promote potato growth and stimulate rhizosphere effect. The present study not only encourages sustainable agriculture through agroecological practices, but also provides broad prospects for the application of PGPM biofertilizer in staple foods.

9.
Front Microbiol ; 15: 1408359, 2024.
Article in English | MEDLINE | ID: mdl-39027097

ABSTRACT

Chinese cabbage (Brassica campestris L. syn. B. rapa), a widely cultivated leafy vegetable, faces significant challenges in annual production due to high-temperature stress, which adversely affects plant weight and quality. The need for an effective solution to mitigate these impacts is imperative for sustainable horticulture. This study explored the effects of a novel biofertilizer, natural soil biotin (NSB), on Chinese cabbage under high-temperature conditions. NSB, rich in organic matter-degrading enzymes, was applied to assess its impact on crop yield, growth, nutrient use efficiency, product quality, and safety. The study also examined the soil microbial community response to NSB application, particularly the changes in the rhizosphere soil's fungal population. The application of NSB led to an increase in the abundance of Oleomycetes, which was associated with a decrease in the diversity and abundance of harmful fungi in the rhizosphere soil. This microbial shift promoted the growth of Chinese cabbage, enhancing both plant weight and quality by fostering a more favorable growth environment. Furthermore, NSB was found to reduce lipid peroxidation in Chinese cabbage leaves under high-temperature stress (40°C/30°C, 16 h/8 h, 24 h) by boosting antioxidant enzyme activity and osmoregulatory substance content. The findings suggest that the NSB application offers a promising approach to environmentally friendly cultivation of Chinese cabbage during high-temperature seasons. It contributes to improving the crop's adaptation to climate change and soil degradation, supporting the development of sustainable agricultural practices. The integration of NSB into agricultural practices presents a viable strategy for enhancing the resilience of Chinese cabbage to high-temperature stress, thereby potentially increasing yield and improving the quality of the produce, which is crucial for the advancement of sustainable horticulture.

10.
Article in English | MEDLINE | ID: mdl-39078554

ABSTRACT

The issue of heavy metal pollution such as nickel poses a significant environmental concern, exerting detrimental effects on the growth and viability of plant life. Plants have various mechanisms to effectively manage heavy metal stress, including the ability to modify their amino acid type and content. This adaptive response allows plants to mitigate the detrimental effects caused by excessive heavy metal accumulation. The aim of this study was to investigate the effect of biofertilizers on nickel accumulation, nitrogen metabolism and amino acid profile of corn (Zea mays L.) cv. 'PL438' exposed to Ni stress. After disinfecting and soaking in water for 24 h, corn seeds were primed with bacterial biofertilizers (T2: NPK + FZ), fungal biofertilizers (T3: Arbuscular mycorrhizal fungi (AMF) + Trichoderma (T)), or a combination of them (T4: NPK + FZ + AMF + T) and were cultured by the hydroponic method in completely controlled conditions. Then, they were simultaneously exposed to nickel chloride at various rates (0, 75, or 150 µM) at the three-leaf stage. They were harvested two weeks later and were subjected to the measurement of Ni content, nitrate and nitrite content, nitrate reductase activity, and amino acid profile by high-performance liquid chromatography. The results showed that the application of Ni at higher rates increased Ni, nitrate, and nitrite contents and nitrate reductase activity. The study of Ni accumulation and TF revealed that Ni accumulated in the roots to a greater extent than in the shoots and TF was < 1 in all treatments. The shoot amino acid profile showed that the treatment of Ni+2 increased som amino acids such as aspartic acid, asparagine, serine, histidine, and glycine versus the control, whereas T4 Ni+2 increased aspartic acid, glutamic acid, threonine and arginine. The change in amino acids in Ni-treated plants may play a key role in their adaptation to Ni stress. The findings indicate that biofertilizers played a crucial role in mitigating the negative impacts of Ni on corn plants through alterations in amino acid composition and decreased absorption and translocation of Ni.

11.
Curr Res Microb Sci ; 7: 100259, 2024.
Article in English | MEDLINE | ID: mdl-39076833

ABSTRACT

Strain CMT1 was isolated from nodules of non-inoculated Roundup Ready (RR) soybean plants (Glycine max L. Merrill), which were collected in fields in Itauguá, Paraguay. The genome of this strain had 338,984,909 bp; 59.2 % G + C content; 377648 bp N50; 5 L50; 55 contigs; 51 RNAs and 5,272 predicted coding DNA sequences (CDS) distributed in 327 subsystems. Based on overall genome-relatedness indices (OGRIs), this strain was taxonomically affiliated with Agrobacterium pusense. Based on genome mining, strain CMT1 is a promising plant growth-promoting bacterium that could be validated in agricultural fields for increasing soybean yield and quality, diminishing the economic, environmental, and health costs of non-sustainable food production.

12.
Appl Microbiol Biotechnol ; 108(1): 370, 2024 Jun 11.
Article in English | MEDLINE | ID: mdl-38861018

ABSTRACT

Members of the genus Lysinibacillus attract attention for their mosquitocidal, bioremediation, and plant growth-promoting abilities. Despite this interest, comprehensive studies focusing on genomic traits governing plant growth and stress resilience in this genus using whole-genome sequencing are still scarce. Therefore, we sequenced and compared the genomes of three endophytic Lysinibacillus irui strains isolated from Canary Island date palms with the ex-type strain IRB4-01. Overall, the genomes of these strains consist of a circular chromosome with an average size of 4.6 Mb and a GC content of 37.2%. Comparative analysis identified conserved gene clusters within the core genome involved in iron acquisition, phosphate solubilization, indole-3-acetic acid biosynthesis, and volatile compounds. In addition, genome analysis revealed the presence of genes encoding carbohydrate-active enzymes, and proteins that confer resistance to oxidative, osmotic, and salinity stresses. Furthermore, pathways of putative novel bacteriocins were identified in all genomes. This illustrates possible common plant growth-promoting traits shared among all strains of L. irui. Our findings highlight a rich repertoire of genes associated with plant lifestyles, suggesting significant potential for developing inoculants to enhance plant growth and resilience. This study is the first to provide insights into the overall genomic signatures and mechanisms of plant growth promotion and biocontrol in the genus Lysinibacillus. KEY POINTS: • Pioneer study in elucidating plant growth promoting in L. irui through comparative genomics. • Genome mining identified biosynthetic pathways of putative novel bacteriocins. • Future research directions to develop L. irui-based biofertilizers for sustainable agriculture.


Subject(s)
Bacillaceae , Genome, Bacterial , Genomics , Bacillaceae/genetics , Bacillaceae/metabolism , Base Composition , Multigene Family , Arecaceae/microbiology , Plant Development , Whole Genome Sequencing , Bacteriocins/genetics , Bacteriocins/metabolism , Bacteriocins/biosynthesis , Phylogeny , Plant Growth Regulators/metabolism , Indoleacetic Acids/metabolism , Stress, Physiological
13.
Microbiol Res ; 285: 127776, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38820701

ABSTRACT

Applying beneficial microorganisms (BM) as bioinoculants presents a promising soil-amendment strategy while impacting the native microbiome, which jointly alters soil-plant performance. Leveraging the untapped potential of native microbiomes alongside bioinoculants may enable farmers to sustainably regulate soil-plant systems via natural bioresources. This review synthesizes literature on native microbiome responses to BMs and their interactive effects on soil and plant performance. We highlight that native microbiomes harbor both microbial "helpers" that can improve soil fertility and plant productivity, as well as "inhibitors" that hinder these benefits. To harness the full potential of resident microbiome, it is crucial to elucidate their intricate synergistic and antagonistic interplays with introduced BMs and clarify the conditions that facilitate durable BM-microbiome synergies. Hence, we indicate current challenges in predicting these complex microbial interactions and propose corresponding strategies for microbiome breeding via BM bioinoculant. Overall, fully realizing the potential of BMs requires clarifying their interactions with native soil microbiomes and judiciously engineering microbiome to harness helpful microbes already present within agroecosystems.


Subject(s)
Agriculture , Microbiota , Soil Microbiology , Soil , Microbiota/physiology , Soil/chemistry , Agriculture/methods , Bacteria/classification , Bacteria/metabolism , Bacteria/isolation & purification , Bacteria/genetics , Crops, Agricultural/microbiology , Plants/microbiology , Microbial Interactions
14.
Microb Pathog ; 192: 106690, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38759935

ABSTRACT

The soil comprising organic matter, nutrients, serve as substrate for plant growth and various organisms. In areas where there are large plantations, there is a huge leaf litter fall. The leaf litter upon decomposition releases nutrients and helps in nutrient recycling, for which the soil engineers such as earthworms, ants and termites are important key players. In this context, the present study was conducted to assess the characteristics of the vermicast obtained by vermicomposting neem leaf litter in terms of microbial flora, plant growth promoting properties and antagonistic activities of the vermicast against phytopathogens. Vermicomposting of neem leaf litter was done using two epigeic earthworm species Eisenia fetida and Eudrilus eugeniae. The vermicast exhibited antagonistic potential against plant pathogens. Out of the four vermiwash infusions studied, the 75 % formulation reduced the disease incidence against mealybug by 82 % in the tree Neolamarkia cadamba. The result of the study suggests that vermicast made from neem leaf litter may be a potent combination of a biofertilizer and a pesticide.


Subject(s)
Azadirachta , Fertilizers , Oligochaeta , Pesticides , Plant Leaves , Azadirachta/chemistry , Animals , Oligochaeta/microbiology , Plant Leaves/microbiology , Pesticides/pharmacology , Composting , Soil Microbiology , Soil/chemistry , Plant Diseases/prevention & control , Plant Diseases/microbiology
15.
J Microbiol Methods ; 222: 106952, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38740286

ABSTRACT

The present study was carried out to valorise cereal (rice and wheat) bran for the development of low-cost liquid consortium bioformulation. Different concentrations of bran-based liquid media formulations were evaluated for the growth of consortium biofertilizer cultures (Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.). Among the bran-based formulations, wheat bran-based formulation WB5, exhibited the highest viable cell of 10.68 ± 0.09 Log10 CFU/ml and 12.63 ± 0.04 Log10 CFU/ml for Azotobacter chroococcum and Bacillus subtilis whereas for Pseudomonas sp., rice bran based bioformulation RB5 recorded maximum viability (12.71 ± 0.05 Log10 CFU/ml) after 72 h of incubation. RB51 and WB52liquid formulations were further optimized for enhanced shelf life using 5, 10 and 15 mM of trehalose, 0.05 and 0.1% carboxymethyl cellulose, and 0.5 and 1.0% glycerol. Following the peak growth at 72 h of incubation, a gradual decrease in the viable population of consortium biofertilizer cultures was observed in all the liquid formulations. The WB5 and RB5 formulations with 15 mM trehalose and 0.1% CMC, not only recorded significantly highest cell count of consortium biofertilizer cultures, but also maximally supported multi-functional traits i.e., phosphate and zinc solubilization, ammonia and IAA production up to 150 days. Further evaluation of seedling emergence and growth of wheat (PBW 826) under axenic conditions recorded WB5 amended with 15 mM trehalose-based consortium bioformulation to exhibit maximum emergence and growth of wheat seedlings. This low-cost liquid formulation can be used for large-scale biofertilizer production as a cost-effective liquid biofertilizer production technology.


Subject(s)
Azotobacter , Bacillus subtilis , Culture Media , Dietary Fiber , Fertilizers , Pseudomonas , Bacillus subtilis/growth & development , Pseudomonas/growth & development , Azotobacter/growth & development , Culture Media/chemistry , Oryza/growth & development , Oryza/microbiology , Edible Grain/microbiology , Edible Grain/growth & development , Microbial Viability
16.
Plants (Basel) ; 13(9)2024 Apr 23.
Article in English | MEDLINE | ID: mdl-38732394

ABSTRACT

Soil salinity hampers durum wheat plant growth and development at various stages. The detrimental effects of salinity on plant cellular and physiological processes necessitate strategies to alleviate its negative impact and improve overall crop yield. This study investigates the efficacy of plant growth-promoting rhizobacteria (PGPR) bacteria inoculation in mitigating salinity stress on two durum wheat genotypes with contrasting degrees of salinity tolerance (Tamaroi, salt-sensitive and Line 5004, salt-tolerant) under greenhouse and field conditions. For this purpose, two halotolerant-PGPR strains, Pseudomonas jordanii strain G34 and Oceanobacillus jordanicus strain GSFE11, were utilized for the inoculation. For the greenhouse experiment, the two selected genotypes were subjected to salinity at the flag leaf stage with continuous irrigation with a Hoagland solution supplemented with 50 mM NaCl. Field experiments were conducted across two locations with contrasting salinity levels over two growing seasons. At the end of both experiments, various parameters including total weight, spike weight, grain weight, spike number, spikelet number, grains per spike and thousand kernel weight were measured. The halotolerant PGPRs, P. jordanii strain G34 and O. jordanicus strain GSFE11, proved effective in alleviating salinity-induced adverse effects and enhancing growth under greenhouse and field conditions. However, bacterial inoculation significantly improved growth in the salt-sensitive genotype and such effects were not observed in the tolerant genotype, emphasizing genotype-specific responses. Notably, inoculation with O. jordanicus increased Na+ and Ca2+ uptake in the salt-tolerant "Line 5004" without hindering growth, suggesting one of its potential mechanisms for salt tolerance. This research demonstrates the potential of halotolerant-PGPR inoculation in enhancing durum wheat production in saline environments, but also underscores the importance of understanding genotype-specific responses for tailored interventions.

17.
Article in English | MEDLINE | ID: mdl-38755473

ABSTRACT

Fermented forest litter (FFL) is a bioproduct used as biofertilizer for several decades in Eastern Asia and Latin America. It is locally handcrafted by farmers in anaerobic conditions by fermenting forest litter added with agricultural by-products such as whey, cereal bran, and molasses. The aim of this study was to characterize the FFL process and product through gas and liquid chromatography analyses. It also provides some highlights on the influence of O2 on this solid-state culture. Under anoxic condition, a maximum CO2 production rate (CDPR) of 0.41 mL/h∙g dry matter (dm) was reached after 8 days. The main volatile organic compounds (VOCs) were ethanol and ethyl acetate, with a production rate profile similar to CDPR. After 21 days of culture, no residual sucrose nor lactose was detected. Lactic and acetic acids reached 58.8 mg/g dm and 10.2 mg/g dm, respectively, ensuring the acidification of the matrix to a final pH of 4.72. A metabarcoding analysis revealed that heterolactic acid bacteria (Lentilactobacillus, Leuconostoc), homolactic acid bacteria (Lactococcus), and yeasts (Saccharomyces, Clavispora) were predominant. Predicted genes in the microbiome confirmed the potential link between detected bacteria and acids and VOCs produced. When O2 was fed to the cultures, final pH reached values up to 8.5. No significant amounts of lactic nor acetic acid were found. In addition, a strong shift in microbial communities was observed, with a predominance of Proteobacteria and molds, among which are potential pathogens like Fusarium species. This suggests that particular care must be brought to maintain anoxic conditions throughout the process.

18.
Biol Futur ; 75(2): 251-257, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38578587

ABSTRACT

Arbuscular mycorrhizal fungi (AMF) have been known to enhance plant growth and nutrient uptake. In this study, we investigated the effects of Funneliformis mosseae, Rhizophagus intraradices, and their co-inoculation on the growth and biochemical composition of tomato (Solanum lycopersicum L.) plants. The findings demonstrated that the inoculation of AMF significantly enhanced shoot and root length, shoot and root dry weight, number of fruits per plant, as well as concentrations of anthocyanin, phenolic compounds, and antioxidants in tomato plants. Both individual and co-inoculation of AMF also significantly increased nitrogen, phosphorus, and potassium concentrations in tomato plants. Our findings suggest that AMF can be used as a potential biofertilizer to enhance the growth and biochemical composition of tomato plants.


Subject(s)
Mycorrhizae , Solanum lycopersicum , Solanum lycopersicum/microbiology , Solanum lycopersicum/metabolism , Solanum lycopersicum/growth & development , Mycorrhizae/physiology
19.
Pest Manag Sci ; 80(8): 4110-4124, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38578650

ABSTRACT

BACKGROUND: Bacillus, as a plant-growth-promoting rhizobacteria, can enhance the resistance of plants to phytopathogens. In our study, Bacillus strains showing excellent biocontrol were screened and used to control ginkgo leaf blight (Alternaria tenuissima). RESULTS: Four biocontrol Bacillus strains-Bsa537, Bam337, Bso544, and Bsu503-were selected from 286 isolates based on their capacity to inhibit pathogens and promote plant growth. The four Bacillus strains significantly improved the resistance of ginkgo to leaf blight. This was especially the case when the four strains were used as a mixture, which contributed to a decrease in lesion area of >40%. Hence, a mixture of Bacillus strains was used to control ginkgo leaf blight in the field. Treatment efficiency varied from 30% to 100% (average 81.5%) and was higher than that of the control (-2% to -18%, average - 8.5%); the antioxidant capacity of the treated ginkgo was also stronger. In addition, ginkgo biomass increased as a result of treatment with the Bacillus mixture, including leaf weight, area, thickness, number of lateral roots and root weight. Furthermore, the Bacillus mixture improved the ginkgo rhizosphere soil by boosting the number of beneficial microorganisms, lowering the number of pathogens and hastening soil catabolism. CONCLUSION: The Bacillus mixture improved the health status of ginkgo by protecting it from pathogen attack, promoting its growth and improving the microorganism community in the rhizosphere. This work closes a technological gap in the biological control of ginkgo leaf blight, investigates application methods for compound Bacillus biofertilizers and establishes a framework for the popularity and commercialization of these products. © 2024 Society of Chemical Industry.


Subject(s)
Alternaria , Bacillus , Ginkgo biloba , Plant Diseases , Ginkgo biloba/microbiology , Bacillus/physiology , Plant Diseases/microbiology , Plant Diseases/prevention & control , Alternaria/physiology , Alternaria/drug effects , Disease Resistance , Plant Leaves , Pest Control, Biological/methods
20.
Ying Yong Sheng Tai Xue Bao ; 35(3): 847-857, 2024 Mar 18.
Article in English | MEDLINE | ID: mdl-38646773

ABSTRACT

Crop health directly affects yields and food security. At present, agrochemicals such as fertilizers and pesticides are mainly used in agricultural production to promote crop health. However, long-term excessive utilization of agrochemicals will damage the ecological environment of farmlands and increase the safety risk of agricultural products. It is urgent to explore efficient and environment-friendly agricultural products. Rhizosphere microbiome are considered as the second genome of plants, which are closely related to crop health. Understanding the key functional microbes, microbe-microbe interactions, and plant-microbe interactions are fundamental for exploring the potential of beneficial microbes in promoting crop health. However, due to the heterogeneity and complexity of the natural environment, stimulating the function of indigenous microorganisms remains uncertain. Synthetic microbial community (SynCom) is an artificial combination of two or more different strain isolates of microorganisms, with different taxonomic, genetic, or functional characteristic. Because of the advantages of maintaining species diversity and community stability, SynCom has been widely applied in the fields of human health, environmental governance and industrial production, and may also have great potential in promoting crop health. We summarized the concept and research status of SynCom, expounded the principles and methods of constructing SynCom, and analyzed the research on the promotion of crop health by exploring the mechanism of plant-microbe interactions, promoting plant growth and development, and improving stress resistance. Finally, we envisaged the future prospects to guide the using SynCom to improve crop health.


Subject(s)
Crops, Agricultural , Microbiota , Rhizosphere , Crops, Agricultural/growth & development , Crops, Agricultural/microbiology , Soil Microbiology , Synthetic Biology/methods , Agriculture/methods
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