The Effect of Demineralization and Remineralization Cycles on the Bond Strength of Resin Composite to Enamel.

Toothwear is a growing concern, particularly in young adults. Prevention strategies remain of utmost importance and may involve the use of remineralising agents. If restorative treatment is indicated, adhesive techniques are recommended. It is unknown whether remineralising agents affect adhesion. METHOD: 77 bovine incisors were subjected to simulated toothwear by erosion (0.3% citric acid) and abrasion (oscillating toothbrush). Samples were randomly arranged into 5 test and 2 control groups (n=11). Each test group had a remineralising agent applied. Subsequently, composite was bonded to each sample and subjected to shear bond testing. Shear bond strength, mode of failure and enamel surface changes were analysed. RESULTS: There was no statistically significant difference for bond strength between groups (p=0.262). Mode of failure was statistically significant between groups (p⟨0.0001). Qualitative analysis showed a surface layer on samples remineralised by calcium silicate and stannous fluoride. Both groups had more adhesive failures. CONCLUSION: Within the limitations of this study, it can be concluded that the remineralising agents tested do not affect shear bond strength though surface layers created on the enamel influence the mode of failure. These 'sacrificial' surface layers have the potential to protect the underlying enamel structure.

Capuchin monkey rituals: an interdisciplinary study of form and function.

Many white-faced capuchin monkey dyads in Lomas Barbudal, Costa Rica, practise idiosyncratic interaction sequences that are not part of the species-typical behavioural repertoire. These interactions often include uncomfortable or risky elements. These interactions exhibit the following characteristics commonly featured in definitions of rituals in humans: (i) they involve an unusual intensity of focus on the partner, (ii) the behaviours have no immediate utilitarian purpose, (iii) they sometimes involve 'sacred objects', (iv) the distribution of these behaviours suggests that they are invented and spread via social learning, and (v) many behaviours in these rituals are repurposed from other behavioural domains (e.g. extractive foraging). However, in contrast with some definitions of ritual, capuchin rituals are not overly rigid in their form, nor do the sequences have specific opening and closing actions. In our 9260 h of observation, ritual performance rate was uncorrelated with amount of time dyads spent in proximity but (modestly) associated with higher relationship quality and rate of coalition formation across dyads. Our results suggest that capuchin rituals serve a bond-testing rather than a bond-strengthening function. Ritual interactions are exclusively dyadic, and between-dyad consistency in form is low, casting doubt on the alternative hypothesis that they enhance group-wide solidarity. This article is part of the theme issue 'Ritual renaissance: new insights into the most human of behaviours'.

Bond Strength of Self-Adhesive Restorative Materials Affected by Smear Layer Thickness but not Dentin Desiccation.

PURPOSE: To use shear bond strength (SBS) testing to determine the effect of surface moisture and smear layer thickness on the adhesion of self-adhesive restorative materials and a universal adhesive. MATERIALS AND METHODS: One single-step self-etch universal adhesive, Prime & Bond Active (PA), was used to bond Ceram.x Spectra ST HV composite resin to dentin and enamel using the self-etching technique. Three commercially available restorative materials and one newly developed material with self-adhesive properties, Activa (A), Fuji II LC(F), Equia Forte (E), and ASAR-MP4 (S), respectively, were also bonded to enamel and dentin prepared moist and dry and to dentin prepared with a thick smear layer. Shear bond testing was performed using an Ultradent bonding apparatus. RESULTS: The universal adhesive generated the highest SBS to dentin and enamel, followed by the newly developed material. None of the materials tested were significantly affected by the moisture conditions on enamel or dentin. The thickness of smear layer significantly affected SBS to dentin for S, F, and E. However, S and F still exhibited higher shear bond strength to dentin with the thicker smear layer compared to the other self-adhesive materials. Only the universal adhesive in self-etch mode was not affected by the thicker smear layer and maintained significantly higher SBS. CONCLUSION: None of the materials tested were affected by bonding to overdried dentin or enamel. All of the self-adhesive materials exhibited lower SBS to specimens with a thicker smear layer. The newly developed material ASAR-MP4 compared favorably to the other self-adhesive materials tested under all test conditions.

Evaluation of Bond Strength of Aesthetic Type of Posts at Different Regions of Root Canal after Application of Adhesive Resin Cement.

AIM: This study aimed to evaluate the bond strength between esthetic posts and dentin at different regions of the root canal in passive mode or push-out active mode. METHODS: Twenty extracted human bicuspid single canal teeth were used in this study. Teeth were sectioned below the cement-enamel junction. The roots of teeth were endodontically treated. Glass fibre posts (Glassix plus, Harald Nordin SA, Switzerland) and zirconia posts (Zirix, Harald Nordin) were then adhesively luted with total-etch (Variolink N, Ivoclar Vivadent, Schaan, Liechtenstein) and self-adhesive (Multilink Speed, Ivoclar Vivadent) resin cement. The roots were divided into two main groups of 10 samples each, according to the type of post used. Each group subdivided into 2 subgroups of 5 samples each, according to the type of cement used. The specimens were transversally sectioned into three slices of 2 mm thickness to perform the push-out test. The push-out tests were performed at crosshead-speed, 0.5 mm/min). Failure modes were evaluated using a scanning electron microscope at magnification (x 150). RESULTS: The results revealed that push-out bond strengths were significantly affected by the type of luting agent and the type of post (P ≤ 0 .05). The mean push-out bond strength values for fibre post were significantly higher than those for zirconia post independent of the luting strategy used. The score values of total-etch adhesive resin cement were higher than those for self-adhesive resin cement irrespective of other variables. Regarding the effect of the root segment on push-out bond strength, results revealed that bond strength decreased from the coronal to the apical section. The cement-dentin interface found to be the weakest part of the root-cement-post unit. CONCLUSION: Glass fibre posts revealed better results in all root third when they adhesively luted with total-etch or self-etch adhesive resin cement and provided significantly increased bond strength compared to the zirconia posts.

Comparative evaluation of the effect of different disinfecting agents on bond strength of composite resin to dentin using two-step self-etch and etch and rinse bonding systems: An in-vitro study.

OBJECTIVE: This study was carried out to examine the effect of the application of different disinfecting agents on the shear bond strength (SBS) of an etch-and-rinse system. MATERIALS AND METHODS: A total of 120 dentin surfaces of human molars were taken. Sixty samples were taken for immediate (testing was done after 24 h) and delayed (testing was done after 6 months) each. Further, they were divided into three subgroups (n = 20) according to the disinfectant used: Group I: control (no disinfectant); Group II: chlorhexidine based; and Group III: Aloe barbadensis miller (Aloe vera) based. Dentine bonding agent was applied, and resin composite build-ups were done for SBS testing. Samples were examined under scanning electron microscope for observing the gap formation for dentin-restoration interface. SBS results were statistically analyzed. RESULTS: The results of the study showed that both chlorhexidine and aloe vera had improved bond strength as compared to the control group both for immediate and delayed SBSs. CONCLUSION: Thus, aloe vera can be used as an alternative to chlorhexidine for cavity disinfection.

In vitro and in vivo comparison of orthodontic indirect bonding resins: A prospective study.

OBJECTIVE: The aim of this study was to evaluate in vitro shear bond strength (SBS) and in vivo bond survival rates of brackets bonded using orthodontic indirect bonding resins. MATERIALS AND METHODS: For the in vitro study, the first group was direct bonding control group. In Groups II and III, bonding was performed with indirect bonding resins that were either chemically or light-cured. The SBS of each sample was examined. For the in vivo study, full-mouth brackets were placed in 20 patients using a split-mouth approach, with either chemically-cured or a light-cured indirect bonding resin. The patients were followed for 12 months. Data were statistically evaluated using analysis of variance, Tukey's tests, and Weibull survival analysis. RESULTS: The mean SBS values (MPa) were 17.6 ± 6.6, 13.1 ± 4.7, and 15.1 ± 5.9 for Group I, Groups II, and III, respectively, (P < 0.05). The adhesive remnant index scores of the groups were generally Score 3 and Score 4. In vivo follow-up showed no statistically significant differences in total bond failure rate between groups (P > 0.05). CONCLUSIONS: In vitro study showed lower SBS with chemically-cured indirect bonding resin than flowable light-cured resin and the control group, but in vivo failure rates of both indirect resins were found to be adequate for clinical usage.

Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing.

INTRODUCTION: The main objective of adhesive dentistry is to create an effective, durable union between the tooth structure and restorative material. However, degradation of adhesive dentine interface remains largely responsible for the relatively short lifetime of tooth colored resin restoration. AIM: The aim of the study is to compare the dentin collagen stabilization property of Chlorhexidine (CHX) and Aloe barbadensis Miller using shear bond strength testing. MATERIALS AND METHODS: Occlusal reduction was done in sixty extracted human mandibular molars to expose the mid coronal dentin and divided into three groups n = 20. Following the surface pretreatment (Group 1 = control, Group 2 = CHX, Group 3 = Aloevera), dentine bonding agent and composite resin were applied and cured. The specimens were then subjected to shear bond strength testing. RESULTS: From the results analyzed, it was noted that there was statistically significant difference between the groups Control and CHX and Control and A. barbadensis Miller (P < 0.05), specifically the values of Control < CHX and Control < A. barbadensis Miller (P < 0.05). However, there was no statistically significant difference between CHX and A. barbadensis Miller (P > 0.05). Hence, the following result for the shear bond strengths to dentin was obtained: Control < CHX ≈ A. barbadensis Miller. CONCLUSION: CHX and A. barbadensis Miller, as pretreatment agents of acid demineralized dentin collagen, has no adverse effect on the immediate shear bond strength of a two-step etch and rinse adhesive to dentin.

Effect of Jig Design and Assessment of Stress Distribution in Testing Metal-Ceramic Adhesion.

PURPOSE: In testing adhesion using shear bond test, a combination of shear and tensile forces occur at the interface, resulting in complex stresses. The jig designs used for this kind of test show variations in published studies, complicating direct comparison between studies. This study evaluated the effect of different jig designs on metal-ceramic bond strength and assessed the stress distribution at the interface using finite element analysis (FEA). MATERIALS AND METHODS: Metal-ceramic (Metal: Ni-Cr, Wiron 99, Bego; Ceramic: Vita Omega 900, Vita) specimens (N = 36) (diameter: 4 mm, veneer thickness: 4 mm; base diameter: 5 mm, thickness: 1 mm) were fabricated and randomly divided into three groups (n = 12 per group) to be tested using one of the following jig designs: (a) chisel (CH) (ISO 11405), (b) steel strip (SS), (c) piston (PI). Metal-ceramic interfaces were loaded under shear until debonding in a universal testing machine (0.5 mm/min). Failure types were evaluated using scanning electron microscopy (SEM). FEA was used to study the stress distribution using different jigs. Metal-ceramic bond strength data (MPa) were analyzed using ANOVA and Tukey's tests (α = 0.05). RESULTS: The jig type significantly affected the bond results (p = 0.0001). PI type of jig presented the highest results (MPa) (p < 0.05) (58.2 ± 14.8), followed by CH (38.7 ± 7.6) and SS jig type (23.3 ± 4.2) (p < 0.05). Failure types were exclusively a combination of cohesive failure in the opaque ceramic and adhesive interface failure. FEA analysis indicated that the SS jig presented slightly more stress formation than with the CH jig. The PI jig presented small stress concentration with more homogeneous force distribution compared to the CH jig where the stress concentrated in the area where the force was applied. CONCLUSION: Metal-ceramic bond strength was affected by the jig design. Accordingly, the results of in vitro studies on metal-ceramic adhesion should be evaluated with caution. CLINICAL SIGNIFICANCE: When adhesion of ceramic materials to metals is evaluated in in vitro studies, it should be noted that the loading jig type affects the results. Clinical observations should report on the location and type of ceramic fractures in metal-ceramic reconstructions so that the most relevant test method can be identified.

Effect of Resin Cement Porosity on Retention of Glass-Fiber Posts to Root Dentin: An Experimental and Finite Element Analysis

The aim of this study was to evaluate the effect of porosity of self-adhesive resin on the stress distribution, post retention and failure mode of fiber post cemented to human root dentin. Ten human central upper incisors with circular root canal were selected. They were sectioned with 15 mm and were endodontically filled. The roots were scanned using micro-CT after post space preparation for root filling remaining evaluation. Fiber posts were cemented using self-adhesive resin cement (Rely X U200, 3M-ESPE). Two 1-mm-thick slices from the cervical, medium and apical thirds were scanned for resin cement bubbles volume measurements and submitted to a push-out test (PBS). Three operators using stereomicroscopy and confocal laser microscopy classified the failure mode. Stress distributions during the push-out test were analyzed using 3D finite element analysis. PBS values (MPa) were submitted to one-way ANOVA and Tukey's post hoc tests and the failure modes using the Kappa coefficient to assess inter-operator agreement. Chi-square test was used to determine significant differences between the methods ( = 0.05). Push-out bond strength was significantly affected by the bubbles presence in all root depth (p<0.05). The stress concentration was higher when the bubbles were present. Adhesive dentin/resin cement interface failure was the most frequent type of failure. Confocal microscopy was better than stereomicroscopy for failure analysis. Bubbles generated during resin cement insertion into the root canal negatively affect the stress distribution and the bond strength. The use of confocal microscopy is recommended for failure analysis.

Resumo O objetivo deste estudo foi avaliar o efeito da integridade do cimento resinoso autoadesivo, expresso pela presença de bolhas, sobre a distribuição de tensão, resistência adesiva e modo de falha de pinos de fibra cimentados à dentina radicular humana. Dez incisivos centrais superiores humanos com canais radiculares circulares foram selecionados. Os mesmos foram seccionados com 15 mm e tratados endodonticamente. As raízes foram digitalizadas utilizando micro-CT após preparo do pino para avaliação de remanescentes de material obturador. Pinos de fibra foram cimentados utilizando cimento autoadesivo (Rely X U200, 3M-ESPE). Duas fatias de 1 mm de espessura dos terços cervical, médio e apical foram escaneados para mensuração do volume de bolhas no cimento resinoso e submetidos ao teste de push-out. Três operadores classificaram o modo de falha utilizando microscopia confocal à laser e lupa estereoscópica. Distribuição de tensão foi analisada pelo método de elementos finitos 3D. Os valores de resistência adesiva (MPa) foram submetidos ao teste ANOVA em fator único seguido do teste de Tukey. Foi utilizado o coeficiente de Kappa para avaliar a concordância entre operadores. O teste Qui-quadrado foi utilizado para determinar diferenças significativas entre os métodos (α=0,05). A resistência adesiva foi significativamente afetada pela presença de bolhas independentemente da profundidade radicular (p<0,05). A concentração de tensão foi maior na presença de bolhas. Maior frequência de falha adesiva ocorreu na interface cimento/dentina. A microscopia confocal foi melhor do que estereomicroscopia para análise de falhas. A presença de bolhas afetou negativamente a distribuição de tensão e a resistência de união. Recomenda-se uso de microscopia confocal para análise de falhas.

Investigating failure behavior and origins under supposed "shear bond" loading.

PURPOSE: This study evaluated failure behavior when resin-composite cylinders bonded to dentin fractured under traditional "shear" testing. Failure was assessed by scaling of failure loads to changes in cylinder radii and fracture surface analysis. Three stress models were examined including failure by: bonded area; flat-on-cylinder contact; and, uniformly-loaded, cantilevered-beam. METHODS: Nine 2-mm dentin occlusal dentin discs for each radii tested were embedded in resin and bonded to resin-composite cylinders; radii (mm)=0.79375; 1.5875; 2.38125; 3.175. Samples were "shear" tested at 1.0mm/min. Following testing, disks were finished with silicone carbide paper (240-600grit) to remove residual composite debris and tested again using different radii. Failure stresses were calculated for: "shear"; flat-on-cylinder contact; and, bending of a uniformly-loaded cantilevered beam. Stress equations and constants were evaluated for each model. Fracture-surface analysis was performed. RESULTS: Failure stresses calculated as flat-on-cylinder contact scaled best with its radii relationship. Stress equation constants were constant for failure from the outside surface of the loaded cylinders and not with the bonded surface area or cantilevered beam. Contact failure stresses were constant over all specimen sizes. Fractography reinforced that failures originated from loaded cylinder surface and were unrelated to the bonded surface area. CONCLUSIONS: "Shear bond" testing does not appear to test the bonded interface. Load/area "stress" calculations have no physical meaning. While failure is related to contact stresses, the mechanism(s) likely involve non-linear damage accumulation, which may only indirectly be influenced by the interface.