Human health risks associated to trace elements and metals in commercial fish from the Brazilian Amazon.

Fish constitutes the main protein source for the Amazonian population. However, the impact of different anthropogenic activities on trace element and metal accumulation in fish and their risks for human health at a regional scale remain largely unexplored. Here we assessed exposure levels of 10 trace elements and metals (Cr, Mn, Fe, Ni, Cu, Zn, As, Cd, Pb, and Hg) in 56 samples belonging to 11 different species of fish from the Brazilian Amazon. We studied the relationship between exposure levels, fish origin, and fish feeding habits, and assessed toxicological and carcinogenic risks for the Amazonian population. No significant correlation was found between sampling site and exposure levels to the studied elements, but a significant difference was found between the accumulation of some metals and the position of the fish species in the food chain. The concentrations of Cr and Hg in fish flesh were found to exceed the Brazilian limits for human consumption. This study shows that current fish consumption patterns can lead to estimated daily intakes of Hg, As and Cr that exceed the oral reference dose, thus posing a toxicological concern. Furthermore, carcinogenic risks may be expected due to the continued exposure to Cr and As. The results of this study show that the consumption of wild caught fish in the Amazon region should be controlled. Moreover, continued monitoring of trace element and metal contamination in fish and on the health of the Amazonian population is recommended, particularly for riverine and indigenous communities.

Ecohydraulics-based environmental flow assessment in two arid North African rivers.

North Africa is among the most water-stressed regions in the world; still, the habitat requirements of its freshwater biota are largely unknown. In this study, (i) we developed habitat suitability curves (HSCs) for freshwater macroinvertebrates in two poorly studied, regulated North African rivers (Ziz and Oum Er-Rbia), and (ii) assessed environmental flows downstream of each river dam by incorporating the HSCs in two-dimensional ecohydraulic models. We demonstrate a low-cost sampling methodology combined with freely distributed ecohydraulic modeling software. The results showed that macroinvertebrates in the arid-desert Ziz River could tolerate a wide range of habitats in terms of flow velocity and water depth compared to the arid-steppe Oum Er-Rbia River, probably due to their adaptation to extreme (arid-desert) environmental conditions. Optimal environmental flows downstream of the Al Hassan Addakhil (Ziz River) and the Al Massira (Oum Er-Rbia River) dams were 1 m3/s and 2 m3/s, respectively. However, environmental flows at 0.5 m3/s and 1 m3/s, respectively, could still maintain sustainable freshwater biota downstream of the dams. The results further highlight the critical status of the Ziz River, which was completely dry, and the alarming status of the Oum Er-Rbia River due to the significant reduction in the water levels of the Al Massira Dam. In a continuously changing climate, we suggest that the proposed environmental flows should be immediately delivered to prevent droughts and ensure healthy freshwater communities downstream of the dams, within a basin-wide freshwater management framework. In this water scarce region, more research is necessary to increase ecological awareness about these understudied freshwater systems and achieve a balance between human needs and ecosystem requirements.

A global systematic map of knowledge of inland commercial navigation effects on freshwater ecosystems.

Inland navigation is one of the most sustainable transport alternatives to help decarbonise the world economy. However, the likely impacts of intensifying inland navigation on freshwater ecosystems are difficult to predict. A global map of knowledge that considers both abiotic and biotic responses to increasing shipping traffic and developing infrastructures is lacking. Deriving general evidence-based assessments is challenging, because most studies on inland navigation impacts are merely descriptive and either consist of local case studies, or address single navigation stressors or specific taxa only. We conducted a systematic mapping of the published literature (1908-2021) to provide a global synthesis of the effects of inland navigation on the biotic and abiotic components of freshwater ecosystems. We show that only half of the reported navigation-related impacts were statistically tested. Navigation itself (vessel operation) had mainly negative effects on native taxa (57%), followed by waterway management (40%), and navigation infrastructures (35%). Navigation has direct negative impacts caused by physical disturbances such as vessel-induced waves, and indirect impacts that facilitate the spread of aquatic invasive species, and altering the abiotic habitat conditions. Thirty percent of the tested relationships showed non-significant impacts on the biotic environment, while in 10% of cases impacts were context-dependent. We identified the main gaps of knowledge, namely (i) impacts of waterway management on communities, (ii) underlying processes of navigation impacts on river ecosystems; and (iii) interactions between multiple navigation factors and cascading effects on multi-taxa responses. These future research directions should improve the diagnosis, mitigate the negative impacts of navigation on rivers and provide guidelines for improving navigated river management.

Biofilms inactivate the free-living stage of Batrachochytrium dendrobatidis, the most destructive pathogen for vertebrate diversity.

Emerging infectious diseases threaten biodiversity and human health. Many emerging pathogens have aquatic life stages and all immersed substrates have biofilms on their surface, i.e., communities of microorganisms producing a gelatinous matrix. However, the outcome of the interactions between environmental biofilms and pathogens is poorly understood. Here we demonstrate that biofilms reduce the survival of the most impactful pathogen for vertebrate diversity, the invasive chytrid fungus Batrachochytrium dendrobatidis. Effects on its zoospores varied with biofilm composition in controlled settings and biofilm compositional variation also coincided with divergent impacts of chytridiomycosis on amphibian populations in nature. Our results suggest that biofilms form a biotic component of ecosystem resistance to Batrachochytrium dendrobatidis by reducing environmental transmission, and that they could be used to develop nature-based technologies to limit the impacts and spread of this invasive chytrid fungus. Our study warrants further research into the interactions between environmental biofilms and pathogenic and/or invasive micro-organisms.

Bending the curve of global freshwater biodiversity loss: what are the prospects?

Freshwater biodiversity conservation has received substantial attention in the scientific literature and is finally being recognized in policy frameworks such as the Global Biodiversity Framework and its associated targets for 2030. This is important progress. Nonetheless, freshwater species continue to be confronted with high levels of imperilment and widespread ecosystem degradation. An Emergency Recovery Plan (ERP) proposed in 2020 comprises six measures intended to "bend the curve" of freshwater biodiversity loss, if they are widely adopted and adequately supported. We review evidence suggesting that the combined intensity of persistent and emerging threats to freshwater biodiversity has become so serious that current and projected efforts to preserve, protect and restore inland-water ecosystems may be insufficient to avert substantial biodiversity losses in the coming decades. In particular, climate change, with its complex and harmful impacts, will frustrate attempts to prevent biodiversity losses from freshwater ecosystems already affected by multiple threats. Interactions among these threats will limit recovery of populations and exacerbate declines resulting in local or even global extinctions, especially among low-viability populations in degraded or fragmented ecosystems. In addition to impediments represented by climate change, we identify several other areas where the absolute scarcity of fresh water, inadequate scientific information or predictive capacity, and a widespread failure to mitigate anthropogenic stressors, are liable to set limits on the recovery of freshwater biodiversity. Implementation of the ERP rapidly and at scale through many widely dispersed local actions focused on regions of high freshwater biodiversity and intense threat, together with an intensification of ex-situ conservation efforts, will be necessary to preserve native freshwater biodiversity during an increasingly uncertain climatic future in which poorly understood, emergent and interacting threats have become more influential. But implementation of the ERP must be accompanied by measures that will improve water, energy and food security for humans - without further compromising the condition of freshwater ecosystems. Unfortunately, the inadequate political implementation of policies to arrest widely recognized environmental challenges such as climate change do not inspire confidence about the possible success of the ERP. In many parts of the world, the Anthropocene future seems certain to include extended periods with an absolute scarcity of uncontaminated surface runoff that will inevitably be appropriated by humans. Unless there is a step-change in societal awareness of - and commitment to - the conservation of freshwater biodiversity, together with necessary actions to arrest climate change, implementation of established methods for protecting freshwater biodiversity may not bend the curve enough to prevent continued ecosystem degradation and species loss.

High-altitude aquatic ecosystems offer faster aging rate of plastics.

While research on the aging behavior of plastics in aquatic systems is extensive, studies focusing on high-altitude ecosystems, characterized by higher solar radiation and lower temperatures, remain limited. This study investigated the long-term aging behavior of non-biodegradable plastics (non-BPs), namely polyethylene terephthalate (PET) and polypropylene (PP) and biodegradable plastics (BPs), specifically polylactic acid plus polybutylene adipate-co-terephthalate (PLA + PBAT) and starch-based plastic (SBP), in a tributary of the Yarlung Zangbo River on the high-altitude Tibetan Plateau. Over 84 days of field aging, all four types of plastics exhibited initial rapid aging followed by deceleration. This aging process can be divided into two phases: rapid surface oxidation aging and an aging plateau phase. Notably, PP aged at a rate comparable to BPs, contrary to expectations of faster aging for BPs. Compared to low-altitude aquatic ecosystems, plastics in this study showed a faster aging rate. This was primarily due to intense ultraviolet radiation causing severe photoaging. Furthermore, the lower temperatures contributed to the formation of thinner biofilms. These thinner biofilms exhibited a reduced capacity to block light, further exacerbating the photoaging process of plastics. Statistical analysis results indicated that temperature, total nitrogen TN, and total phosphorus TP were likely the main water quality parameters influencing plastic aging. The varying effects of water properties and nutrients underscore the complex interaction of water quality parameters in high-altitude environments. Given the delicate nature of the high-altitude environment, the environmental impact of plastics, especially BPs, warrants careful consideration.

Combined effects of heat waves and pesticide pollution on zooplankton communities: Does the timing of stressor matter?

Most studies assessing the combined effects of chemical and non-chemical stressors on aquatic ecosystems have been based on synchronous stressor applications. However, asynchronous exposure scenarios may be more common in nature, particularly for pulsed stressors such as heatwaves and pesticide concentration peaks. In this study, we investigated the single and combined effects of the insecticide chlorpyrifos (CPF) and a heatwave (HW) on a zooplankton community representative of a Mediterranean coastal wetland using synchronous (CPF+HW) and asynchronous (HW→CPF and CPF→HW) exposure scenarios. CPF was applied at a concentration of 0.8 µg/L (single pulse), and the HW was simulated by a temperature increase of 8°C above the control temperature (20°C) for 7 days in freshwater microcosms. The interaction between stressors in synchrony resulted in synergistic effects at the population level (Daphnia magna) and additive at the community level. The partial reduction of sensitive species resulted in an abundance increase of competing species that were more tolerant to the evaluated stressors (e.g. Moina sp.). The asynchronous exposure scenarios resulted in a similar abundance decline of sensitive populations as compared to the synchronous one; however, the timing of stressor resulted in different responses in the long term. In the HW→CPF treatment, the D. magna population recovered at least one month faster than in the CPF+HW treatment, probably due to survival selection and cross-tolerance mechanisms. In the CPF→HW treatment, the effects lasted longer than in the CPF+HW, and the population did not recover within the experimental period, most likely due to the energetic costs of detoxification and effects on internal damage recovery. The different timing and magnitude of indirect effects among the tested asynchronous scenarios resulted in more severe effects on the structure of the zooplankton community in the CPF→HW treatment. Our study highlights the relevance of considering the order of stressors to predict the long-term effects of chemicals and heatwaves both at the population and community levels.

Real-Time Detection of Microplastics Using an AI Camera.

Microplastics (MPs, size ≤ 5 mm) have emerged as a significant worldwide concern, threatening marine and freshwater ecosystems, and the lack of MP detection technologies is notable. The main goal of this research is the development of a camera sensor for the detection of MPs and measuring their size and velocity while in motion. This study introduces a novel methodology involving computer vision and artificial intelligence (AI) for the detection of MPs. Three different camera systems, including fixed-focus 2D and autofocus (2D and 3D), were implemented and compared. A YOLOv5-based object detection model was used to detect MPs in the captured image. DeepSORT was then implemented for tracking MPs through consecutive images. In real-time testing in a laboratory flume setting, the precision in MP counting was found to be 97%, and during field testing in a local river, the precision was 96%. This study provides foundational insights into utilizing AI for detecting MPs in different environmental settings, contributing to more effective efforts and strategies for managing and mitigating MP pollution.

Climate change vulnerability of Arctic char across Scandinavia.

Climate change is anticipated to cause species to shift their ranges upward and poleward, yet space for tracking suitable habitat conditions may be limited for range-restricted species at the highest elevations and latitudes of the globe. Consequently, range-restricted species inhabiting Arctic freshwater ecosystems, where global warming is most pronounced, face the challenge of coping with changing abiotic and biotic conditions or risk extinction. Here, we use an extensive fish community and environmental dataset for 1762 lakes sampled across Scandinavia (mid-1990s) to evaluate the climate vulnerability of Arctic char (Salvelinus alpinus), the world's most cold-adapted and northernly distributed freshwater fish. Machine learning models show that abiotic and biotic factors strongly predict the occurrence of Arctic char across the region with an overall accuracy of 89 percent. Arctic char is less likely to occur in lakes with warm summer temperatures, high dissolved organic carbon levels (i.e., browning), and presence of northern pike (Esox lucius). Importantly, climate warming impacts are moderated by habitat (i.e., lake area) and amplified by the presence of competitors and/or predators (i.e., northern pike). Climate warming projections under the RCP8.5 emission scenario indicate that 81% of extant populations are at high risk of extirpation by 2080. Highly vulnerable populations occur across their range, particularly near the southern range limit and at lower elevations, with potential refugia found in some mountainous and coastal regions. Our findings highlight that range shifts may give way to range contractions for this cold-water specialist, indicating the need for pro-active conservation and mitigation efforts to avoid the loss of Arctic freshwater biodiversity.

Strong Saharan Dust Deposition Events Alter Microbial Diversity and Composition in Sediments of High-Mountain Lakes of Sierra Nevada (Spain).

Mediterranean high-mountain lakes are being increasingly affected by strong Saharan dust deposition events. However, the ecological impacts of these severe atmospheric episodes remain largely unknown. We examined the effects of a strong Saharan dust intrusion to the Iberian Peninsula in 2022 on the physicochemical parameters and prokaryotic communities in sediments of nine high-mountain lakes of Sierra Nevada (Spain) located above 2800 m.a.s.l and in different orientations (north vs. south). A previous year (2021), with lower Saharan dust deposition with respect to 2022, was used for interannual comparisons. The strong dust deposition to the high-mountain lakes resulted in a significant increase in sediment nutrient availability which was linked to changes in the composition of prokaryotic communities. Decreases in alpha diversity and changes in beta diversity of prokaryotic communities were mainly observed in lakes located in the south compared to the north orientation likely because the former was more affected by the atmospheric dust deposition episode. Dust intrusion to the high-mountain lakes resulted in significant changes in the relative abundance of specific genera involved in important nutrient cycling processes such as phosphate solubilization, nitrogen fixation, nitrification, and denitrification. Saharan dust deposition also increased predicted microbial functionality in all lakes. Our findings show that severe atmospheric dust inputs to remote high-mountain lakes of Sierra Nevada can have significant biogeochemical and biodiversity consequences through changes in nutrient availability and prokaryotic communities in sediments of these freshwater ecosystems. This information contributes to understanding how Mediterranean high-mountain lakes of Sierra Nevada face strong intrusions of Saharan dust and their ecological consequences.

Anticipating responses to climate change and planning for resilience in California's freshwater ecosystems.

As human-caused climate changes accelerate, California will experience hydrologic and temperature conditions different than any encountered in recorded history. How will these changes affect the state's freshwater ecosystems? Rivers, lakes, and wetlands are managed as a water resource, but they also support a complex web of life, ranging from bacteria, fungi, and algae to macrophytes, woody plants, invertebrates, fish, amphibians, reptiles, birds, and mammals. In much of the state, native freshwater organisms already struggle to survive massive water diversions and dams, deteriorating water quality, extensive land cover modification for agriculture and urban development, and invasions of exotic species. In the face of climate change, we need to expand efforts to recover degraded ecosystems and to protect the resilience, health, and viability of existing ecosystems. For this, more process-based understanding of river, lake, and wetlands ecosystems is needed to forecast how systems will respond to future climate change and to our interventions. This will require 1) expanding our ability to model mechanistically how freshwater biota and ecosystems respond to environmental change; 2) hypothesis-driven monitoring and field studies; 3) education and training to build research, practitioner, stewardship, and policy capabilities; and 4) developing tools and policies for building resilient ecosystems. A goals-driven, hypothesis-informed collaboration among tribes, state (and federal) agencies, nongovernmental organizations, academicians, and consultants is needed to accomplish these goals and to advance the skills and knowledge of the future workforce of practitioners, regulators, and researchers who must live with the climate changes that are already upon us and will intensify.

Multiple antibiotic resistance and herbicide catabolic profiles of bacteria isolated from Lake Villarrica surface sediments (Chile).

Antibiotics and herbicides are contaminants of emerging concern in aquatic environments. Lake Villarrica is a relevant freshwater body in Chile and was recently designated a 'saturated nutrient zone'. Here, we investigated the occurrence of multiple antibiotic resistance (MAR) and herbicide catabolic profiles among bacteria present in the surface sediments of Lake Villarrica. The occurrence of antibiotic-resistant genes (ARGs; blaTEM, catA and tetM) and herbicide-catabolic genes (HCGs; phnJ and atzA) was investigated by qPCR. Subsequently, the presence of culturable bacteria with multiple resistance to amoxicillin (AMX), chloramphenicol (CHL) and oxytetracycline (OXT) was studied. Forty-six culturable MAR (AMX + CHL + OXT) strains were isolated and characterized with respect to their resistance to 11 antibiotics by using a disc diffusion assay and testing their ability to use herbicides as a nutrient source. qPCR analyses revealed that ARGs and HCGs were present in all sediment samples (101 to 103 gene copies g-1), with significant (P ≤ 0.05) higher values in sites near Villarrica city and cattle pastures. The plate method was used to recover MAR isolates from sediment (103-106 CFU g-1), and most of the 46 isolates also showed resistance to oxacillin (100%), cefotaxime (83%), erythromycin (96%) and vancomycin (93%). Additionally, 54 and 57% of the MAR isolates were able to grow on agar supplemented (50 mg L-1) with atrazine and glyphosate as nutrient sources, respectively. Most of the MAR isolates were taxonomically close to Pseudomonas (76.1%) and Pantoea (17.4%), particularly those isolated from urbanized sites (Pucón city). This study shows the presence of MAR bacteria with herbicide catabolic activity in sediments, which is valuable for conservation strategies and risk assessments of Lake Villarrica. However, major integrative studies on sediments as reservoirs or on the fate of MAR strains and traces of antibiotics and herbicides as a result of anthropic pressure are still needed.

Species on the move: Impacts of climate change on the spatial range of endemic fishes of the eco-sensitive semi-arid area of the Arabian Peninsula.

Climate change is one of the most significant challenges worldwide in the Anthropocene, and it is predicted to importantly affect biological diversity, especially in freshwaters. Freshwater fishes are facing considerable global threats, particularly in eco-sensitive semi-arid to arid areas such as the Arabian Peninsula, which is considered a highly stressed region in the Middle East. Endemic species are believed to display a narrow range of traits, with rarity reflecting adaptation to specific environmental regimes, and they are thus highly sensitive to environmental disturbances. This study is the first attempt to map the occurrence of endemic freshwater fish species and predict the impact of climate change on their spatial range in the semi-arid area of the Arabian Peninsula using Species Distribution Modeling (SDM). We compared the present and future (2041-2060 and 2061-2080) climate niche for the species under various climatic scenarios. All global circulation models (GCMs) performed well in predicting the species' climatic niche (AUC ranging between 0.72 and 0.92). For certain species (Cyprinion acinaces, Garra buettikeri, Carasobarbus exulatus, Arabibarbus arabicus, and Cyprinion mhalense), variables associated with precipitation were more important than those related to temperature, while for others (Carasobarbus apoensis, G. sahilia, G tibanica, and Aphaniops kruppi), temperature-related variables were most important. Precipitation in the coldest quarter and in the driest quarter was the most sensitive variable for the predictions. The species showed distinct responses to climate change; seven were predicted to lose their climatically suitable habitats (losers) and are thus threatened and highly vulnerable to the effects of climate change, while two species were predicted to expand their range (winners). Regular monitoring of fish in the Arabian Peninsula is recommended to conserve endemic species and their ecosystems.

Temperature extremes nip invasive macrophyte Cabomba caroliniana A. Gray in the bud: potential geographic distributions and risk assessment based on future climate change and anthropogenic influences.

Cabomba caroliniana A. Gray, an ornamental submerged plant indigenous to tropical America, has been introduced to numerous countries in Europe, Asia, and Oceania, impacting native aquatic ecosystems. Given this species is a popular aquarium plant and widely traded, there is a high risk of introduction and invasion into other environments. In the current study the potential global geographic distribution of C. caroliniana was predicted under the effects of climate change and human influence in an optimised MaxEnt model. The model used rigorously screened occurrence records of C. caroliniana from hydro informatic datasets and 20 associated influencing factors. The findings indicate that temperature and human-mediated activities significantly influenced the distribution of C. caroliniana. At present, C. caroliniana covers an area of approximately 1531×104 km2 of appropriate habitat, especially in the south-eastern parts of South, central and North America, Southeast Asia, eastern Australia, and most of Europe. The suitable regions are anticipated to expand under future climate scenarios; however, the dynamics of the changes vary between different extents of climate change. For example, C. caroliniana is expected to expand to higher latitudes, following global temperature increases under SSP1-2.6 and SSP2-4.5 scenarios, however, intolerance to temperature extremes may mediate invasion at higher latitudes under future extreme climate scenarios, e.g., SSP5-8.5. Owing to the severe impacts its invasion causes, early warning and stringent border quarantine processes are required to guard against the introduction of C. caroliniana especially in the invasion hotspots such as, Peru, Italy, and South Korea.

Community science for assessing the vulnerability of freshwater ecosystems: Water quality monitoring, restoration, and outreach by young naturalists in Nova Scotia, Canada.

Community science models that complement formal scientific investigations are valuable tools for addressing gaps in knowledge and engaging the community. Freshwater quality monitoring and vulnerability assessment, for example, are essential for conserving freshwater ecosystems, but often suffer from limited resources. While municipal programs focus on priority areas, community-based models can improve overall coverage both spatially and temporally. As an example of how community science initiatives centered on freshwater ecosystems enhance monitoring capacity, we present the case of the Young Naturalists Club (YNC) in Nova Scotia, Canada. The YNC's Nature Guardians program involves youth aged 10-14 in water monitoring, restoration, and outreach activities within Shubie Park, in Dartmouth, Nova Scotia. Between 2018 and 2021 the Nature Guardians collected water monitoring data at multiple park locations, and shared findings with city authorities and the Atlantic Water Network. In response to high bacteria counts, and concerns over nutrient influxes, the group's 2021-2023 restoration efforts have aimed to improve water quality, focusing on native plantings and outreach signage. This type of community-based monitoring offers several advantages, including local site selection based on community concerns, the potential for low-cost long-term monitoring, and community engagement. While a community-based monitoring model presents certain challenges including data standardization and verification, it offers a broader reach and can produce high-quality data when appropriate protocols are followed. This case underscores the potential of a community-based water quality monitoring approach and highlights the potential for community science to augment existing assessment structures, ultimately contributing to more resilient and sustainable freshwater ecosystems.

Ecological implications and drivers of emerging contaminants in Dongting Lake of Yangtze River Basin, China: A multi-substance risk analysis.

Emerging contaminants (ECs) are increasingly recognized as a global threat to biodiversity and ecosystem health. However, the cumulative risks posed by ECs to aquatic organisms and ecosystems, as well as the influence of anthropogenic activities and natural factors on these risks, remain poorly understood. This study assessed the mixed risks of ECs in Dongting Lake, a Ramsar Convention-classified Typically Changing Wetland, to elucidate the major EC classes, key risk drivers, and magnitude of anthropogenic and natural impacts. Results revealed that ECs pose non-negligible acute (30% probability) and chronic (70% probability) mixed risks to aquatic organisms in the freshwater lake ecosystem, with imidacloprid identified as the primary pollutant stressor. Redundancy analysis (RDA) and structural equation modeling (SEM) indicated that cropland and precipitation were major drivers of EC contamination levels and ecological risk. Cropland was positively associated with EC concentrations, while precipitation exhibited a dilution effect. These findings provide critical insights into the ecological risk status and key risk drivers in a typical freshwater lake ecosystem, offering data-driven support for the control and management of ECs in China.

Mercury biomagnification in the food web of Agami Pond, Kaw-Roura Nature Reserve, French Guiana.

Freshwater ecosystems are among the most important ecosystems worldwide, however, over the last centuries, anthropogenic pressures have had catastrophic effects on them. Mercury (Hg) is one of the main environmental contaminants which globally affect ecosystems and particularly freshwater wildlife. While Hg originates from natural sources, anthropogenic activities such as agriculture, biomass combustion, and gold mining increase its concentrations. Gold mining activities are the main drivers of Hg emission in tropical ecosystems and are responsible for up to 38% of global emissions. Once in its methylated form (MeHg), mercury biomagnifies through the trophic chain and accumulates in top predators. Due to the toxicity of MeHg, long-lived predators are even more subjected to chronic effects as they accumulate Hg over time. In the present study we quantified Hg contamination in two top predators, the Black caiman Melanosuchus niger and the Agami heron Agamia agami, and in their prey in the Kaw-Roura Nature Reserve in French Guiana and evaluated the biomagnification rate in the trophic chain. Our results show that despite a TMF in the range of others in the region (4.38 in our study), top predators of the ecosystem present elevated concentrations of Hg. We have found elevated Hg concentrations in the blood of adult Black caiman (2.10 ± 0.652 µg g-1 dw) and chicks of Agami heron (1.089 ± 0.406 µg g-1 dw). These findings highlight the need to better evaluate the potential impact of Hg in freshwater top predators, especially regarding reprotoxic effects.

Comparison of different macroinvertebrates bioassessment indices in a large near-natural watershed under the context of metacommunity theory.

The metacommunity theory proposes that community structure and biodiversity are influenced by both local processes (such as environmental filtering) and regional processes (such as dispersal). Despite the extensive use of traditional bioassessments based on species-environment relationships, the impact of dispersal processes on these assessments has been largely overlooked. This study aims to compare correlations between various bioassessment indices, including Shannon Weiner (H'), Biological Monitoring Working Party (BMWP), average score per taxon (ASPT), biotic index (BI), and EPT taxa index (EPT), based on macroinvertebrates collected from 147 sampling sites in a subtropical Chinese near-natural catchment. Modified indices were calculated by removing species strongly influenced by dispersal processes to address the influence of dispersal processes. Their relationship with environmental factors was then compared to the original indices. The study employed random forest regression (RFR) to compare the explanatory power of environmental factors using the two sets of indices. The spearman rank correlation analysis was conducted to examine the correlation between indices and environmental factors. The river health assessment was performed based on both modified and original indices. The results reveal significant differences between original and modified indices (especially H' and BI) providing a more accurate reflection of environmental conditions. Furthermore, the sensitivity of the different indices to various environmental factors varied, leading to differences in the bioassessment results between the modified and the original indices. Notably, original H', BMWP, and ASPT overestimated the bioassessment results, whereas the original BI underestimated them. These findings offer valuable insights into bioassessment and river health assessment evaluation within the catchment and other interconnected freshwater ecosystems, such as lakes, reservoirs, and wetlands. Our study underscores the importance of assessing and mitigating the impact of dispersal processes on bioassessment to obtain a more precise representation of the status of freshwater ecosystems.

Global natural concentrations of Rare Earth Elements in aquatic organisms: Progress and lessons from fifty years of studies.

Rare Earth Elements (REEs) consist of a coherent group of elements with similar physicochemical properties and exhibit comparable geochemical behaviors in the environment, making them excellent tracers of environmental processes. For the past 50 years, scientific communities investigated the REE concentrations in biota through various types of research (e.g. exploratory studies, environmental proxies). The extensive development of new technologies over the past two decades has led to the increased exploitation and use of REEs, resulting in their release into aquatic ecosystems. The bioaccumulation of these emerging contaminants has prompted scientific communities to explore the fate of anthropogenic REEs within aquatic ecosystems. To achieve this, it is necessary to determine the natural concentration levels of REEs in aquatic organisms and the factors controlling REE dynamics. However, knowledge gaps still exist, and no comprehensive approach currently exists to assess the REE concentrations at the ecosystem scale or the factors controlling these concentrations in aquatic organisms. Based on a database comprising 102 articles, this study aimed to: i) provide a retrospective analysis of research topics over a 50-year period; ii) establish reference REE concentrations in several representative phyla of aquatic ecosystems; and iii) examine the global-scale influences of habitat and trophic position as controlling factors of REE concentrations in organisms. This study provides reference concentrations for 16 phyla of freshwater or marine organisms. An influence of habitat REE concentrations on organisms has been observed on a global scale. A trophic dilution of REE concentrations was highlighted, indicating the absence of biomagnification. Lastly, the retrospective approach of this study revealed several research gaps and proposed corresponding perspectives to address them. Embracing these perspectives in the coming years will lead to a better understanding of the risks of anthropogenic REE exposure for aquatic organisms.

Presence of pharmaceutical contaminants of emerging concerns in two rivers of western Cuba and their relationship with the extracellular enzymatic activity of microbial communities.

In recent years, the concern derived from the presence of emerging contaminants in the environment and the possible effects on the One Health trilogy has increased. This study determined the concentration of pharmaceutical contaminants of emerging concern and their relationship with the extracellular enzymatic activity of microbial communities from two rivers in western Cuba. Two sampling stations were analyzed; one in the Almendares River (urban) and the other in the San Juan River (rural), taking into account the pollution sources that arrive at these stations and previous physicochemical characterizations. Extracellular protease, acid phosphatase, alkaline phosphatase, lipase, and catalase activities in water and sediments were determined and correlated with contaminants of emerging concern determined by liquid chromatography with mass spectrometry. This study evidenced the presence of different pharmaceutical contaminants found in the categories of antihypertensives, stimulants, anti-inflammatories, and antibiotics in both rivers. Concentrations of contaminants of emerging concern were greater in the Almendares River compared to the San Juan River. In addition, through the canonical redundancy analysis, the influence of these contaminants on the extracellular enzymatic activities of microbial communities was documented, where in most cases they inhibit protease, phosphatase, and lipase activities and enhance catalase activity in response to oxidative stress. The present investigation constitutes the first report in Cuba of the presence of pharmaceutical contaminants of emerging concern and one of the few works that exist in the Latin American region.