ABSTRACT
The high silicon content in rice plant waste, specifically rice husks, makes this waste by-product attractive for the extraction and valorization of silicon oxide, which is widely used as an inert support in catalysis, drug delivery and molecular sieving. The procedures currently used for the treatment of plant biomass make extensive use of mineral acids (HCl, H2SO4, HNO3), which, besides them being potential environmental pollutants, reduce the yield and worsen the chemical-physical properties of the product. In this study, an evaluation of the easy treatment of rice husks by benchmarking different, more eco-friendly carboxylic acids in order to obtain a mesoporous SiO2 with an alveolar structure and a relatively high surface area and pore volume (300-420 m2/g, 0.37-0.46 cm3/g) is presented. The obtained mesoporous silicas are characterized by worm-like pores with a narrow size distribution and a maximum in the range of 3.4-3.5 nm. The mesoporous structure of the obtained materials was also confirmed by TEM. The complete removal of the organic part of the rice husks in the final materials was evidenced by thermogravimetric analysis. The high purity of the obtained mesoporous silica was detected using ICP analysis (98.8 wt. %). The structure peculiarities of the obtained mesoporous silicas were also characterized by solid-state NMR and ATR-FTIR spectroscopies. The morphology of the mesoporous silica was investigated by SEM.
Subject(s)
Oryza , Silicon Dioxide , Oryza/chemistry , Silicon Dioxide/chemistry , Porosity , Spectroscopy, Fourier Transform Infrared , ThermogravimetryABSTRACT
Solid-state fermentation represents a sustainable approach for the conversion of agro-industrial wastes into high-added-value feed ingredients. The present study aimed to evaluate the effects of the dietary addition of a solid-state-fermented mixture of olive mill stone waste (OMSW) and Lathyrus clymenum husks (LP) on the antioxidant blood parameters of weaned piglets. Two hundred 35-day-old weaned piglets were allotted into two groups and fed either a control (C) diet or a diet containing 50 g of OMSW-LP per kg (OMSW-LP) for 40 days. Blood samples were collected at 35 and 75 days of age to assess the free radical scavenging activity (FRSA), reduced glutathione (GSH) levels, catalase activity (CAT), protein carbonyls (CARBs), and thiobarbituric acid reactive species (TBARS). The OMSW-LP diet reduced the TBARS (p = 0.049) and CARB contents (p = 0.012) and increased the levels of FRSA (p = 0.005), GSH (p = 0.040), and CAT activity (p = 0.012) in the piglets' blood, likely due to the synergistic action of the antioxidants and bioactive compounds present in the OMSW-LP mixture. Overall, the dietary inclusion of solid-state-fermented OMSW-LP at 50 g/kg could potentially serve a bio-functional purpose since it enhanced the antioxidant blood parameters in this study, a crucial factor for the health and growth of piglets post-weaning.
ABSTRACT
Rice husks are a low value byproduct, even though it possesses molecules with great potential, such as arabinoxylans, proteins, and silica. These molecules can be used to improve mechanical and physicochemical properties of materials for food packaging. In this work, hydrothermal treatment was used for a sustainable extraction of the valuable molecules present in rice husks. Various extraction temperatures (180, 200, and 220 °C) were performed targeting to extract fractions with distinct compositions. The water extract obtained at 220 °C demonstrated the highest extraction yield, 3 times superior to conventional hot water extraction. These extracts exhibited high content of proteins, phenolic compounds, and carbohydrates, particularly arabinoxylans. This extract was incorporated in chitosan-based films in different ratios, 1:0.1, 1:0.3, and 1:0.5 (chitosan:extract, w:v). The film with the lowest extract ratio presented the highest flexibility (higher elongation and lower Young's modulus) when compared to the pristine chitosan film. The antioxidant capacity was also increased, achieving an antioxidant capacity of >10-fold in comparison to control film. The results revealed that hydrothermal extraction emerges as an environmentally friendly and sustainable methodology for extracting valuable compounds from rice industry byproducts. This method exhibits significant potential to impart flexible and antioxidant properties to biobased materials.
Subject(s)
Antioxidants , Chitosan , Oryza , Oryza/chemistry , Chitosan/chemistry , Antioxidants/chemistry , Antioxidants/isolation & purification , Phenols/chemistry , Phenols/isolation & purification , Water/chemistry , Temperature , Plant Extracts/chemistry , Chemical Fractionation/methodsABSTRACT
Rice husks are rich in xylan, which can be hydrolyzed by xylanase to form xylooligosaccharides (XOS). XOS are a functional oligosaccharide such as improving gut microbiota and antioxidant properties. In this study, the structure and functional characteristics of XOS were studied. The optimal xylanase hydrolysis conditions through response surface methodology (RSM) were: xylanase dosage of 3000â¯U/g, hydrolysis time of 3â¯h, hydrolysis temperature of 50⯰C. Under this condition, the yield of XOS was 150.9â¯mg/g. The TG-DTG curve showed that XOS began to decompose at around 200⯰C. When the concentration of XOS reached 1.0â¯g/L, the clearance rate of DPPH reached 65.76â¯%, and the scavenging rate of OH reached 62.10â¯%, while the clearance rate of ABTS free radicals reached 97.70â¯%, which was equivalent to the clearance rate of VC. XOS had a proliferative effect on four probiotics: Lactobacillus plantarum, Lactobacillus brucelli, Lactobacillus acidophilus, and Lactobacillus rhamnosus. However, the further experiments are needed to explore the improvement effect of XOS on human gut microbiota, laying a foundation for the effective utilization of XOS. XOS have a wide range of sources, low price, and broad development prospects. The reasonable utilization of XOS can bring greater economic benefits.
Subject(s)
Antioxidants , Glucuronates , Oligosaccharides , Oryza , Probiotics , Oligosaccharides/pharmacology , Oligosaccharides/chemistry , Oryza/chemistry , Glucuronates/pharmacology , Glucuronates/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Hydrolysis , Endo-1,4-beta Xylanases/metabolism , LactobacillusABSTRACT
This study presents a novel approach for biorefining oat husks into furfural, leveraging a unique pilot-scale setup. Unlike conventional furfural manufacturing processes, which often result in substantial cellulose degradation and environmental concerns associated with sulfuric acid usage, our method utilizes phosphoric acid as a catalyst to achieve high furfural yield while minimizing cellulose destruction. Drawing on our research conducted in a distinctive pilot-scale environment, we successfully developed and implemented a tailored biorefining process for oat husks. Through meticulous experimentation, we attained a remarkable furfural yield of 11.84% from oven-dried mass, accompanied by a 2.64% yield of acetic acid. Importantly, our approach significantly mitigated cellulose degradation, preserving 88.31% of the cellulose content in oat husks. Existing catalytic (H2SO4) furfural manufacturing processes often lead to substantial cellulose degradation (40-50%) in lignocellulosic leftover during the pretreatment stage. As a result of the research, it was also possible to reduce the destruction of cellulose in the lignocellulose leftover to 11.69% of the output (initial) cellulose of oat husks. This research underscores the feasibility and sustainability of utilizing oat husks as a valuable feedstock for furfural production, highlighting the potential of phosphoric acid as a catalyst in biorefining processes. By showcasing our unique pilot-scale methodology, this study contributes to advancing the field of environmentally friendly biorefining technologies.
ABSTRACT
Coffee processing generates large amounts of residues of which a portion still has bioactive properties due to their richness in phenolic compounds. This study aimed to obtain a coffee husks extract (CHE) and to encapsulate it (ECHE) with polyvinylpyrrolidone using a one-step procedure of solid dispersion. The extraction and encapsulation yields were 9.1% and 92%, respectively. Thermal analyses revealed that the encapsulation increased the thermal stability of CHE and dynamic light scattering analyses showed a bimodal distribution of size with 81% of the ECHE particles measuring approximately 711 nm. Trigonelline and caffeine were the main alkaloids and quercetin the main phenolic compound in CHE, and the encapsulation tripled quercetin extraction. The total phenolics content and the antioxidant activity of ECHE, assayed with three different procedures, were higher than those of CHE. The antioxidant activity and the bioaccessibility of the phenolic compounds of ECHE were also higher than those of CHE following simulated gastrointestinal digestion (SGID). Both CHE and ECHE were not toxic against Alliumcepa cells and showed similar capacities for inhibiting the pancreatic α-amylase in vitro. After SGID, however, ECHE became a 1.9-times stronger inhibitor of the α-amylase activity in vitro (IC50 = 8.5 mg/mL) when compared to CHE. Kinetic analysis revealed a non-competitive mechanism of inhibition and in silico docking simulation suggests that quercetin could be contributing significantly to the inhibitory action of both ECHE and CHE. In addition, ECHE (400 mg/kg) was able to delay by 50% the increases of blood glucose in vivo after oral administration of starch to rats. This finding shows that ECHE may be a candidate ingredient in dietary supplements used as an adjuvant for the treatment of diabetes.
Subject(s)
Antioxidants , Coffea , Rats , Animals , Antioxidants/analysis , Quercetin , Povidone , Coffea/chemistry , KineticsABSTRACT
The study investigated the effect of modifying rapeseed husks with ammonia and epichlorohydrin on their sorption capacity against anionic reactive dyes: Reactive Black 5 (RB5) and Reactive Yellow 84 (RY84). Its scope included sorbents characterization (FTIR, pHPZC), determination of pH influence on the sorption effectiveness of dyes, the adsorption kinetics of dyes, as well as the maximum sorption capacity. The study proved that the reaction of rapeseed husk biomass with ammonia can lead to its amination, namely to the introduction of amine functional groups into the material's structure. The sorption effectiveness of RB5 and RY84 on the tested sorbents was the highest in the pH range of 2-3. The dye sorption kinetics was well described by the pseudo-second-order model. The sorption equilibrium time ranged from 90 to 180 min, and depended on the initial concentration of dyes and the number of amino groups on the sorbent's surface. The most efficient of the sorbents tested were rapeseed husks pre-activated with epichlorohydrin and then aminated with ammonia. Their sorption capacity determined for RB5 and RY84 was 135.83 mg/g and 114.23 mg/g, respectively, which was 794% and 737% higher than that of the non-modified husks.
ABSTRACT
Agricultural by-products like rice husk, bran, and spray corn husks, often utilized as feed, are considered less desirable. This study aims to enhance the utilization rate of these materials by subjecting then to liquid hot water (LHW) pretreatment, followed by enzymatic hydrolysis to produce fermentable sugars. We investigated the production of L-lactic acid using two methods: simultaneous saccharification fermentation (SSF) and separate hydrolysis fermentation (SHF), following varying intensities of LHW pretreatment. The results showed that the optimal enzymatic hydrolysis efficiency was achieved from spray corn husks under the pretreatment conditions of 155 °C and 15 min. SHF was generally more effective than SSF. The glucose L-lactic acid conversion rate in SHF using spray corn husks can reach more than 90 %. Overall, this work proposed a novel, environmental-friendly strategy for efficient and for L- lactic acid production from spray corn husks.
Subject(s)
Cellulose , Zea mays , Zea mays/metabolism , Cellulose/metabolism , Lactic Acid , Fermentation , Water , HydrolysisABSTRACT
OBJECTIVE: This study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet. METHODS: Five healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed. RESULTS: Supplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea. CONCLUSION: Supplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.
ABSTRACT
BACKGROUND: Rice, a pivotal global food staple, annually accumulates vast amounts of rice husks, resulting in substantial environmental impact. Exploiting the high silica content in rice husk, our research aimed to recycle this agricultural byproduct to synthesize mesoporous silica nanoparticles (rMSNs). These nanoparticles were further modified to evaluate their potential as effective carriers for cancer drug delivery. RESULTS: rMSNs showed high biocompatibility, large surface area and porous structure as MSNs, making them excellent drug carriers. Further modifications were applied to rMSNs, such as the incorporation of the lanthanides europium and gadolinium into rMSNs, making them fluorescent and magnetic for detection and tracking using confocal fluorescence microscopy and magnetic resonance imaging. Additionally, folic acid and aptamer AS1411 were conjugated with rMSNs to enhance the targeting of cancer cells. HeLa cells exhibited higher uptake of camptothecin (CPT)-loaded rMSNs compared to normal fibroblast cells (L929). The linkage of disulfide bonds to rMSNs also allowed CPT to be carried by rMSNs and released intracellularly in the presence of the abundant reducing agent glutathione. The validation of rMSNs in vitro and in vivo proved their practical feasibility. CONCLUSION: Our findings indicate that low-cost rMSNs, derived from recycled agricultural waste, can replace highly valuable MSNs. Functionalized rMSNs exhibit promising capabilities in transporting clinical drugs to specific aberrant tissues and offering dual-targeting and dual-imaging functionalities for enhanced cancer therapy. © 2023 Society of Chemical Industry.
Subject(s)
Antineoplastic Agents , Nanoparticles , Neoplasms , Oryza , Humans , Silicon Dioxide/chemistry , HeLa Cells , Drug Delivery Systems , Drug Carriers/chemistry , Nanoparticles/chemistry , Porosity , Neoplasms/diagnostic imaging , Neoplasms/drug therapyABSTRACT
A huge amount of textile dyes are released as industrial waste into the environment each year, which alters the water's natural appearance and causes toxicity and carcinogenicity in the human body. Peanut husk is considered an agro-waste and contains many valuable compounds, such as cellulose. Different concentrations of cellulose were extracted from peanut husk and then loaded with bio-silver nanoparticles, which were fabricated using neem leaves (Azadirachta indica) as a reducing agent to form Ag-cellulose nanocomposites (Ag-Cell-NCMs). Different devices were used to characterize Ag-Cell-NCMs. The TEM images displayed that the size of Ag-Cell-NCMs ranged between 13.4 and 17.4 nm after dye adsorption. The Ag-Cell-NCMs were used to adsorb toxic dyes such as crystal violet (CV). Different parameters were applied, such as the ratio of cellulose to Ag-NPs, pH, contact time, adsorbent dose, dye concentration, and the temperature required to reach the optimization conditions to remove CV dye from the aqueous solution. Different kinetics and isotherm models were applied to the experimental data to explain the mechanism of the adsorption process. The adsorption of CV on Ag-Cell-NCMs follows the pseudo-second order, and the best-fit isotherm was the Langmuir isotherm. The new composite was tested for the possibility of dye desorption and ability to be reused several times, and we found that the new nanocomposite can be reused for multiple adsorptions and there is a possibility of dye desorption.
ABSTRACT
The effects of steam explosion (SE) pretreatment on the structural properties of lignin isolated from coconut husk (CH) biomass via soda pulping were investigated in this work. The isolated SE lignin was classified as dilute acid impregnation SE lignin (ASEL), water impregnation SE lignin (WSEL), and 2-naphthol impregnation SE lignin (NSEL). The various types of functional groups isolated from SE lignin were characterized and compared using a variety of complementary analyses: FTIR spectroscopy, NMR spectroscopy, GPC chromatography, HPAEC-PAD chromatography and thermal analyses. It was revealed that ASEL has the highest solid recovery with 55.89 % yield as well as the highest sugars content compared to WSEL (45.66 % yield) and NSEL (49.37 % yield). Besides, all isolated SE lignin contain a significant quantity of non-condensed G-type and S-type units but less amount of H-type units as supported by previous research. The SE lignin produced lignin with higher molecular weight (Mw ASEL: 72725 g mol-1 > Mw WSEL: 13112 g mol-1 > Mw NSEL: 6891 g mol-1) seems to influence the success of the synthesis reaction of phenolic resins. Because of the large variances in the physicochemical properties of SE lignin polymers, their structural properties were increased toward numerous alternative techniques in lignin-based applications.
Subject(s)
Lignin , Steam , Lignin/chemistry , Cocos , Phenol , Magnetic Resonance Spectroscopy , HydrolysisABSTRACT
The area of sunflower crops is steadily increasing. A beneficial way of managing sunflower waste biomass could be its use as a feedstock for biochar production. Biochar is currently being considered as an additive for improving soil parameters, including the ability to oxidise methane (CH4) - one of the key greenhouse gases (GHG). Despite the high production of sunflower husk, there is still insufficient information on the impact of sunflower husk biochar on the soil environment, especially on the methanotrophy process. To fill this knowledge gap, an experiment was designed to evaluate the effects of addition of sunflower husk biochar (produced at 450-550 °C) at a wide range of doses (1-100 Mg ha-1) to Haplic Luvisol. In the presented study, the CH4 oxidation potential of soil with and without sunflower husk biochar was investigated at 60 and 100% water holding capacity (WHC), and with the addition of 1% CH4 (v/v). The comprehensive study included GHG exchange (CH4 and CO2), physicochemical properties of soil (pH, soil organic carbon (SOC), dissolved organic carbon (DOC), nitrate nitrogen (NO3--N), WHC), and the structure of soil microbial communities. That study showed that even low biochar doses (5 and 10 Mg ha-1) were sufficient to enhance pH, SOC, DOC and NO3--N content. Importantly, sunflower husk biochar was significant source of NO3--N, which soil concentration increased from 9.40 ± 0.09 mg NO3--N kg-1 for the control to even 19.40 ± 0.26 mg NO3--N kg-1 (for 100 Mg ha-1). Significant improvement of WHC (by 11.0-12.4%) was observed after biochar addition at doses of 60 Mg ha-1 and higher. At 60% WHC, application of biochar at a dose of 40 Mg ha-1 brought significant improvements in CH4 oxidation rate, which was 4.89 ± 0.37 mg CH4-C kg-1 d-1. Higher biochar doses were correlated with further improvement of CH4 oxidation rates, which at 100 Mg ha-1 was seventeen-fold higher (8.36 ± 0.84 mg CH4-C kg-1 d-1) than in the biochar-free control (0.48 ± 0.28 mg CH4-C kg-1 d-1). CO2 emissions were not proportional to biochar doses and only grew circa (ca.) twofold from 3.16 to 6.90 mg CO2-C kg-1 d-1 at 100 Mg ha-1. Above 60 Mg ha-1, the diversity of methanotrophic communities increased, with Methylobacter becoming the most abundant genus, which was as high as 7.45%. This is the first, such advanced and multifaceted study of the wide range of sunflower husk biochar doses on Haplic Luvisol. The positive correlation between soil conditions, methanotroph abundance and CH4 oxidation confirmed the multifaceted, positive effect of sunflower husk biochar on Haplic Luvisol. Sunflower husk biochar can be successfully used for Haplic Luvisol supplementation. This additive facilitates soil protection against degradation and has the potential to mitigate GHG emissions.
Subject(s)
Greenhouse Gases , Helianthus , Soil/chemistry , Carbon , Methane/analysis , Nitrogen/analysis , Carbon Dioxide/analysis , Charcoal/chemistry , Greenhouse Gases/analysis , Nitrous Oxide/analysisABSTRACT
Rice husk is one of the most plentiful agriculture by-products in rice producing areas, which harbors a substantial proportion of biological metabolites, however, it has not been well studied. As an attempt to utilize it as a productive manner, phytochemical investigation on rice husk has performed and led to the isolation of three undescribed (1, 2, and 7), along with twelve known components (3-6, and 8-15). Those chemical structures were elucidated based on massive spectroscopic methods. Among them, compounds 4, 6-8, and 10-13 have been shown to act as α-glucosidase inhibitors. Notably, the most active compounds, 10/11, demonstrated comparable α-glucosidase inhibitory effect (IC50 = 1.83 ± 0.11 µg/mL) to that of 1-deoxynojirimycin (IC50 = 1.02 ± 0.16 µg/mL). For the molecular docking simulation studies, compounds 10/11 showed relative binding interactions with α-glucosidase enzyme (PDB ID: 3A4A) that similar to those reference inhibitors. Additionally, the crude extract of O. sativa demonstrated better α-glucosidase inhibitory effect to that of isolated components, with the IC50 value at 1.25 ± 0.07 µg/mL.
Subject(s)
Glycoside Hydrolase Inhibitors , Oryza , Oryza/chemistry , Molecular Structure , alpha-Glucosidases/metabolism , Molecular Docking Simulation , Plant Extracts/chemistryABSTRACT
Rice husk is an abundant agricultural waste generated from rice production, but its application is limited. Considering its complex components, the rice husk was hydrolyzed by different enzymes to enhance its saccharification. In this study, saccharification of the rice husk by cellulase, xylosidase, and xylanase was first investigated. The synergistic effect of LPMO on the above hydrolases and different enzyme combinations in the saccharification process was then explored. Thereafter, the formulation of the enzyme cocktail and the degradation conditions were optimized to obtain the highest saccharification efficiency. The results showed that the optimum enzyme cocktail consists of Celluclast 1.5 L (83.3 mg/g substrate), the key enzymes in the saccharification process, worked with BpXyl (20 mg/g substrate), BpXyn11 (24 mg/g substrate), and R17L/N25G (4 mg/g substrate). The highest reducing sugar concentration (1.19 mg/mL) was obtained at pH 6.0 and 60 â for 24 h. Fourier transform infrared spectroscopy and scanning electron microscopy were employed to characterize the structural changes in the rice husk after degradation. The results showed that the key chemical bonds in cellulose and hemicellulose were broken. This study illuminated the concept of saccharifying lignocellulose from rice husk using LPMO synergistically assisted combined-hydrolase including cellulase, xylosidase, and xylanase, and provided a theoretical basis for lignocellulose biodegradation.
Subject(s)
Cellulase , Oryza , Xylosidases , Mixed Function Oxygenases/metabolism , Oryza/metabolism , Polysaccharides/metabolism , Cellulase/metabolism , Xylosidases/metabolismABSTRACT
Physicochemical characteristics of dietary fibers may modulate digesta transit behavior. The present study was conducted to clarify the effect of level and particle size (PS) of insoluble fibers on digesta mean retention time (MRT) in the proximal gastrointestinal tract (mouth-ileocecal junction). Six ileal-cannulated pigs (26.8â ±â 2.08 kg) were assigned to 3 dietary treatments in a 3â ×â 3 replicated Latin-square design. Finely ground (1 mm screen) or coarse (intact) oat husks (OH) and soybean hulls (SBH) were added (50:50, w/w) to a maize-whey protein-wheat-based diet at 50 (low) or 250 g/kg (high) inclusion levels to obtain a low-fine fiber (LF), high-fine fiber (HF), and high-coarse fiber (HC) diet. Markers to follow liquids (Co-EDTA), fine solids (Y3O2), or fibrous particles (Yb-mordanted OH and Cr-mordanted SBH) were given as a single pulse dose and marker concentrations were subsequently measured hourly in digesta for 13 h after administration. Mean retention time values were obtained from the concentration of markers in digesta observed over time by fitting a generalized Michaelis-Menten equation and calculating the time of peak. Fiber addition and fiber particle size neither affected the MRT of liquid nor solid digesta phases (Pâ =â 0.903). Segregation between solid and liquid digesta phases was observed for all diets (Pâ <â 0.0001), although the extent of segregation was greater for LF compared with HF and HC (Pâ =â 0.0220). The MRT of SBH particles, but not of OH-particles was longer for coarse vs fine PS (96 min, Pâ <â 0.05). In conclusion, digesta MRT was influenced by the dietary concentration but not by PS of insoluble fibers. The addition of insoluble fibers reduces digesta phase segregation from mouth to distal ileum in growing pigs.
The velocity with which feed passes through the gastrointestinal tract affects the processes of digestion and absorption of nutrients. There is evidence that the presence of insoluble fibers, as well as their physical and chemical characteristics, can modify digesta transit. A better understanding of the impact of insoluble fibers on digestive processes may lead to better feeding strategies for pigs. In this study, we investigated the effect of addition of insoluble fibers, and their particle size, on digesta transit behavior from ingestion to the end of the small intestine of growing pigs. The results showed that there is less segregation between solid and liquid digesta phases when adding fibers, regardless their particle size. Changes in digesta transit behavior mean that the kinetics of digestion and absorption of nutrients, and consequently, the metabolic use of the nutrients absorbed may be altered.
Subject(s)
Digestion , Upper Gastrointestinal Tract , Swine , Animals , Particle Size , Gastrointestinal Tract , Ileum , Diet/veterinary , Dietary Fiber/pharmacology , Mouth , Animal Feed/analysis , Gastrointestinal TransitABSTRACT
In this study, the sound absorption coefficients of rice and buckwheat husks were estimated. Computed tomography (CT) images were processed to determine the circumference and surface area of voids in the granular material, and the normal incident sound absorption coefficients were derived. In addition, the tortuosity, which expresses the complexity of the sound wave propagation through the structure, was measured for each material. The theoretical sound absorption coefficients were then compared to the measured sound absorption coefficients with and without consideration of the tortuosity. A correction factor was used to bring the surface area of the granular material closer to the actual surface area and observed that the tortuosity obtained theoretical values that matched the trend of the measured values. These results indicate that using CT images to estimate the sound absorption coefficient is a viable approach.
ABSTRACT
The brightly colored synthetic dyes used in the textile industry are discharged at high concentrations-for example, various azo dyes including Methylene Blue (MB) and Methyl Orange (MO)-which is a matter of global concern, as such dyes are harmful to humans and the environment. Microbial degradation is considered an efficient alternative for overcoming the disadvantages of conventional physical and chemical dye removal methods. In this study, we investigated the potential of multiple types of the enzyme-producing extremophilic bacteria Bacillus FW2, isolated from food waste leachate, for the decolorization and bioremediation of artificial synthetic dyes. The screening of enzyme production and assaying of bacterial strain enzymes are essential for enhancing the breakdown of azo bonds in textile azo dyes. The degradation efficiencies of the water-soluble dyes MB and MO were determined at different concentrations using rice husk, which is an efficient substrate. Using the rice husks, the MO was removed completely within 20 h, and an estimated 99.8% of MB was degraded after 24 h by employing shaking at 120 rpm at 40 °C-whereas a removal efficiency of 98.9% was achieved for the combination of MB + MO. These results indicate the possibility of applying an extremophilic bacterial strain, Bacillus sp., for large-scale dye degradation in the future.
ABSTRACT
In this study, conventional and Graphene Oxide-engineered biochars were produced and thoroughly characterized, in order to investigate their potential as adsorptive materials. Two types of biomass, Rice Husks (RH) and Sewage Sludge (SS), two Graphene Oxide (GO) doses, 0.1% and 1%, and two pyrolysis temperatures, 400 °C and 600 °C were investigated. The produced biochars were characterized in physicochemical terms and the effect of biomass, GO functionalization and pyrolysis temperature on biochar properties was studied. The produced samples were then applied as adsorbents for the removal of six organic micro-pollutants from water and treated secondary wastewater. Results showed that the main factors affecting biochar structure was biomass type and pyrolysis temperature, while GO functionalization caused significant changes on biochar surface by increasing the available C- and O- based functional groups. Biochars produced at 600 °C showed higher C content and Specific Surface Area, presenting more stable graphitic structure, compared to biochars produced at 400 °C. Micro-pollutant adsorption rates were in the range of 39.9%-98.3% and 9.4%-97.5% in table water and 28.3%-97.5% and 0.0%-97.5% in treated municipal wastewater, for the Rice Husk and Sewage Sludge biochars respectively. The best biochars, in terms of structural properties and adsorption efficiency were the GO-functionalized biochars, produced from Rice Husks at 600 °C, while the most difficult pollutant to remove was 2.4-Dichlorophenol.
Subject(s)
Environmental Pollutants , Sewage , Sewage/chemistry , Adsorption , Wastewater , Charcoal/chemistry , Water/chemistryABSTRACT
Coffee processing is a major contributor to the creation of food and product waste. Using coffee co-products can play an essential role in addressing environmental problems and issues with nutritionally unbalanced foods, population growth, and food-related diseases. This research aimed to determine the quality and sensory parameters (aw, pH, dry matter, TAC, TPC, fat, fatty acids profile, fiber, caffeine, chlorogenic acids, color, and sensory analysis) of different botanical origins of cascara (coffee husks) and silverskin (thin layer). The results of this study show that silverskin and cascara are a good source of TAC (1S 58.17 ± 1.28%, 2S 46.65 ± 1.20%, 1C 36.54 ± 1.84%, 2C 41.12 ± 2.11%). Cascara showed the presence of polyphenols (2C 49.135 g GAE·kg-1). Coffee co-products are good sources of fiber. Silverskin had higher values of caffeine than cascara. Palmitic, stearic, oleic, linoleic, and arachidic acids were the most represented acids in the samples. Given the obtained results, cascara can be considered "low-fat" (1C 4.240 g·kg-1 and 2C 5.4 g·kg-1). Based on the sensory evaluation, no sample reached the acceptable index value of 70%. Understanding the link between the character, identification properties, and composition of coffee co-products of different botanical origins can enable their application in the food industry.