Integrating Whey Processing: Ultrafiltration, Nanofiltration, and Water Reuse from Diafiltration.

This work proposes an integrated production of whey protein concentrate (WPC) and lactose and the recovery of water from diafiltration (DF) steps. Whey protein and lactose can be concentrated using ultrafiltration and nanofiltration, respectively, and both can be purified using DF. However, DF uses three-fold the initial volume of whey. We propose a method to reclaim this water using reverse osmosis and adsorption by activated carbon. We produced WPC with 88% protein and purified lactose (90%), and 66% of the water can be reclaimed as drinking water. Additionally, the reclaimed water was used to produce another batch of WPC, with no decrease in product quality. Water recovery from the whey process is necessary to meet the needs of a dairy refinery.

Optimizing dsRNA engineering strategies and production in E. coli HT115 (DE3).

Producing double-stranded RNA (dsRNA) represents a bottleneck for the adoption of RNA interference technology in agriculture, and the main hurdles are related to increases in dsRNA yield, production efficiency, and purity. Therefore, this study aimed to optimize dsRNA production in E. coli HT115 (DE3) using an in vivo system. To this end, we designed a new vector, pCloneVR_2, which resulted in the efficient production of dsRNA in E. coli HT115 (DE3). We performed optimizations in the culture medium and expression inducer in the fermentation of E. coli HT115 (DE3) for the production of dsRNA. Notably, the variable that had the greatest effect on dsRNA yield was cultivation in TB medium, which resulted in a 118% increase in yield. Furthermore, lactose induction (6 g/L) yielded 10 times more than IPTG. Additionally, our optimized up-scaled protocol of the TRIzol™ extraction method was efficient for obtaining high-quality and pure dsRNA. Finally, our optimized protocol achieved an average yield of 53.3 µg/mL after the production and purification of different dsRNAs, reducing production costs by 72%.

Potentially functional lactose-free ice cream with Lacticaseibacillus casei CSL3, ginger, and honey.

AIMS: To develop and characterize a functional lactose-free ice cream with added ginger and honey, evaluate the survival of Lacticaseibacillus casei CSL3 under frozen storage and the simulated gastrointestinal tract (GIT), as well as antioxidant activity and product acceptability. METHODS AND RESULTS: The survival of Lacticaseibacillus casei CSL3 was evaluated for 180 days, under frozen storage, and GIT at 60 days. At 15 days of storage, proximal composition, antioxidant activity, color, pH, acidity, fusion, density, overrun, and sensory analysis were performed. Ice cream was an effective food matrix for maintaining the viability of CSL3, with concentrations > 7 log CFU g- 1 during storage and GIT. In addition, the analysis showed overrun and prebiotic characteristics through high values of antioxidant activity and phenolic compounds, good acceptability, and purchase intention. CONCLUSIONS: The product has satisfactory market potential (acceptance rate of 95.19% and purchase intention rate > 96%), and it could become another means of inserting probiotics in food.

Duración de la diarrea aguda infecciosa con el uso de leche deslactosada vs leche con lactosa en la población pediátrica: Revisión sistemática / Duration of acute infectious diarrhea with the use of lactose-free milk vs. lactose-containing milk in the pediatric population: A systematic review

Introducción: La diarrea aguda es una entidad frecuente en pediatría, constituyendo una de las principales causas de mortalidad en países en desarrollo y en niños menores de cinco años. Si bien la alimentación representa uno de los pilares fundamentales en el tratamiento de la misma, no existe consenso entre los profesionales en cuanto a la indicación de leche deslactosada durante el curso del cuadro. Objetivos: Realizar una revisión sistemática para estudiar el impacto del consumo de leche deslactosada vs leche regular en la duración de la diarrea aguda infecciosa en niños. Materiales y métodos: Se realizó una revisión sistemática incluyendo artículos publicados desde el año 2008 al 2023, utilizando para la búsqueda las bases de datos PubMed, Lillacs, Cochrane Library y literatura gris. Se incluyeron estudios experimentales, observacionales, revisiones, guías de atención y metaanálisis, realizados en pacientes pediátricos sin patologías de base, cursando cuadro de diarrea aguda infecciosa, que compararan el uso de leche deslactosada frente a leche regular. Resultados: Se seleccionaron doce artículos. En 9 de ellos se constató una disminución en la duración de la diarrea en los pacientes que recibieron leche deslactosada con una diferencia de medias de 18 horas (en un rango entre 4 y 32.6 horas). No se reportaron diferencias estadísticamente significativas en la mortalidad entre el uso de una u otra fórmula láctea. En relación al uso de una u otra fórmula no se objetivaron variaciones en el peso estadísticamente significativas. La necesidad de hospitalización fue similar entre ambos grupos. Solo un artículo analizó la frecuencia o volumen de deposiciones sin encontrar diferencias significativas (AU)

Introduction:Acute diarrhea is frequent in pediatrics, and constitutes one of the main causes of mortality in developing countries and in children under five years of age. Although feeding is one of the fundamental pillars in the treatment of diarrhea, there is no consensus among professionals regarding the indication of lactose-free milk during the course of the symptoms. Objectives: To conduct a systematic review to study the impact of lactose-free milk vs. regular milk consumption on the duration of acute infectious diarrhea in children. Materials and methods: A systematic review was conducted including articles published between 2008 and 2023, using PubMed, Lillacs, Cochrane Library databases, and gray literature for the search. Experimental and observational studies, reviews, care guidelines and meta-analysis were included, conducted in pediatric patients without underlying diseases, with acute infectious diarrhea, comparing the use of lactose-free milk versus regular milk. Results: Twelve articles were selected. Nine of them showed a decrease in the duration of diarrhea in patients who received lactose-free milk with a mean difference of 18 hours (ranging from 4 to 32.6 hours). No statistically significant differences in mortality were reported between the use of one or the other milk formula. Regarding the use of one or the other formula, there were no statistically significant variations in weight. The need for hospital admission was similar between the two groups. Only one article analyzed stool frequency or volume with no significant differences (AU)

Relationship Between Maternal Age and Macronutrient Content of Colostrum.

BACKGROUND: Little is known about the relationship between maternal age and the macronutrient content of colostrum. RESEARCH AIMS: This study aimed to evaluate the relationship between maternal age and human milk macronutrient content by comparing the concentrations of lactose, proteins, and lipids in the colostrum of women with younger, moderate, and advanced maternal age. METHODS: An observational, cross-sectional study was designed to compare the macronutrient concentrations in the colostrum of women aged < 20 years, 20 to 34 years, and > 34 years (younger, moderate, and advanced maternal age, respectively; n = 33 per group). For each participant, 3 ml of colostrum was collected by manual extraction from the right breast at 10 am, 39-48 hr after delivery, and analyzed using a Miris Human Milk Analyzer. Macronutrient concentrations were compared between the groups using analysis of variance. P < 0.05 was considered significant. RESULTS: Mothers with moderate maternal age had a higher colostrum lipid concentration than those with younger or advanced maternal age (2.3 mg, SD = 1.4 mg vs. 1.5 mg, SD = 1.0 mg vs. 1.6 mg, SD = 0.9 mg, respectively; p = 0.007). Lactose and protein contents in the analyzed samples did not differ among the three study groups. CONCLUSION: This study lends support to the potential variation of lipids in colostrum by maternal age and suggests individual adaptation to the nutritional components of milk to the needs of the infant may be beneficial.

Characterization of variables related to high stability of raw cow milk.

This research paper analyzes the stability of raw cow milk in the alcohol test and seeks to understand to know the factors that influence milk stability and the occurrence of unstable non-acid milk. Milk samples were collected from the cooling tanks of rural farmers in the state of Paraná twice in summer and twice in winter. The farms were classified according to the production system: pasture with supplementation and feedlot. The following variables were analyzed: stability in the alcohol test, titratable acidity, ionized calcium concentration (iCa), chemical composition of milk, somatic cell count and standard plate count. The results showed that milk stability was greater in winter vs. summer, when the milk contained higher iCa, and in the feedlot vs. pasture system. The Pearson Correlation between variables (ethanol stability, milk composition, iCa, cooling tank temperature, milk volume, number of milking, number of cows milked, fat/protein ratio, distance and travel time) were analyzed. Stability was negatively correlated with iCa concentration and positively with lactose content. Logistic regression of the risk of unstable non-acid milk at 72% alcohol (UNAM72) showed that only iCa and lactose were determinants, while evaluation of the same risk at 78% alcohol revealed iCa, titratable acidity, lactose and milk urea nitrogen as risk factors. Under the dairy farming conditions of Paraná state, the frequency of UNAM72 was low (12.16%) and was higher in summer and in pasture systems with supplementation. In conclusion, in dairy herds bred with high technological level, with adequate nutritional and health management, the frequency of UNAM is low and is related to nutritional management abnd, perhaps, heat stress, factors that alter iCa and lactose levels.

Avaliação da concordância entre os resultados da genotipagem do polimorfismo C/T-13910 e do teste de tolerância à lactose: um estudo retrospectivo populacional no Brasil / Evaluation of agreement between c/t-13910 polymorphism genotyping results and lactose tolerance test results: a retrospective population-based study in brazil

ABSTRACT Background: Lactose tolerant test (LTT) is the most broadly used diagnostic test for lactose intolerance in Brazil, is an indirect, minimally invasive and a low-cost test that is widely available in primary care and useful in clinical practice. The C/T-13910 polymorphism in lactase persistence has been well characterized in Caucasian populations, but there are no studies evaluating the concordance between C/T-13910 polymorphism genotyping results and LTT results in Brazil, where the population is highly mixed. Objective: We aimed to evaluate agreement between presence of C/T-13910 polymorphism genotyping and malabsorption in LTT results. Methods: This is a retrospective analysis of a Brazilian population whose data were collected from a single laboratory database present in several Brazilian states. Results of individuals who underwent both genetic testing for lactose intolerance (C/T-13910 polymorphism genotyping) and an LTT from April 2016 until February 2019 were analysed to evaluate agreement between tests. Groups were classified according to age (<10-year-old (yo), 10-17 yo, ≥18 yo groups) and state of residence (São Paulo or Rio Grande do Sul). Results: Among the 404 patients evaluated, there was agreement between the genotyping and LTT results in 325 (80.4%) patients and discordance in 79 (19.6%) patients (k=0.42 -moderate agreement). Regarding the genotype, 47 patients with genotype C/C (lactase nonpersistence) had normal LTT results, and 32 with genotype C/T or T/T (indicating lactase persistence) had abnormal LTT results. Neither age nor state of residence (Rio Grande do Sul or São Paulo) affected the agreement between test results. Conclusion: Considering the moderate agreement between C/T-13910 polymorphism genotyping and LTT results (κ=0.42) in the Brazilian population, we hypothesize that an analysis of other polymorphisms could be a strategy to improve the agreement between genotyping and established tests and suggest that additional studies should focus on exploring this approach.

RESUMO Contexto: O teste de tolerancia à lactose (TTL) é ampliamente utilizado por ser minimamente invasivo e de baixo custo, disponível na atenção primária e muito útil na prática clínica. Está bem estabelecido o polimorfismo C/T-13910 na persistência da lactase em populações caucasianas, mas não há estudos avaliando a concordância entre os resultados da genotipagem do polimorfismo C/T-13910 e do TTL no Brasil, onde a população é altamente miscigenada. Objetivo: Avaliar a concordância entre a presença do polimorfismo C/T-13910 e a má absorção nos resultados do TTL. Métodos: Análise retrospectiva de dados coletados de um laboratorio presente em vários estados brasileiros. Os resultados dos pacientes que realizaram um teste genético para intolerância à lactose (genotipagem do polimorfismo C/T-13910) e um TTL de abril de 2016 a fevereiro de 2019 foram analisados para avaliar a concordância entre os testes. Os grupos foram classificados de acordo com a idade (<10 anos; 10-17 anos, ≥18 anos) e estado de residência (São Paulo ou Rio Grande do Sul). Resultados: Entre os 404 pacientes avaliados, houve concordância entre os resultados de genotipagem e TTL em 325 (80,4%) pacientes e discordância em 79 (19,6%) pacientes (K=0,42 - concordância moderada). Em relação ao genótipo, 47 pacientes com genótipo C/C (não persistência de lactase) apresentaram TTL normal e 32 com genótipo C/T ou T/T (indicando persistência da lactase) apresentaram TTL anormal. A idade e o estado de residência (Rio Grande do Sul ou São Paulo) não afetaram a concordância entre os resultados dos exames. Conclusão: Considerando a concordância moderada entre a genotipagem do polimorfismo C/T-13910 e os resultados de TTL (κ=0,42) na população brasileira, sugerimos que a análise de outros polimorfismos poderia ser uma estratégia para melhorar a concordância entre os testes.

Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process.

Lyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. Bacillus cereus strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolerate 500 ppm of free cyanide, and the presence of genes such as ars, cad, ppa, dap, among others, associated with the bioremediation of toxic compounds and enterotoxins (nheA, nheB, nheC). Following lyophilization, the survival rates for Mannitol 2.5%, Mannitol 10%, and Glucose 1% were 98.02%, 97.12%, and 96.30%, respectively, with the rates being lower than 95% for other sugars. However, during storage, for the same sugars, the survival rates were 78.71%, 97.12%, and 99.97%, respectively. In the cake morphology, it was found that the lyophilized morphology showed no relationship with bacterial survival rate. The best cryoprotectant for the PBC strain was 1% glucose since it maintained constant and elevated bacterial growth rates during storage, ensuring that the unique characteristics of the bacterium were preserved over time. These findings hold significant implications for research as they report a new Bacillus cereus strain with the potential to be utilized in bioremediation processes.

Genotype Prevalence of Lactose Deficiency, Vitamin D Deficiency, and the Vitamin D Receptor in a Chilean Inflammatory Bowel Disease Cohort: Insights from an Observational Study.

Lactose intolerance (LI) and vitamin D deficiency (VDD) have been linked to inflammatory bowel disease (IBD). We conducted an observational study in 192 Chilean IBD patients to investigate the prevalence of a specific gene variant (LCT-13910 CC genotype) associated with LI and the prevalence of VDD/Vitamin D Receptor (VDR) gene variants. Blood samples were analyzed using Illumina's Infinium Global Screening Array. The LCT-13910 CC genotype was found in 61% of IBD patients, similar to Chilean Hispanic controls and lower than Chilean Amerindian controls. The frequency of the LCT-13910-C allele in Chilean IBD patients (0.79) was comparable to the general population and higher than Europeans (0.49). Regarding VDR and VDD variants, in our study, the rs12785878-GG variant was associated with an increased risk of IBD (OR = 2.64, CI = 1.61-4.32; p-value = 0.001). Sixty-one percent of the Chilean IBD cohort have a genetic predisposition to lactose malabsorption, and a significant proportion exhibit genetic variants associated with VDD/VDR. Screening for LI and VDD is crucial in this Latin American IBD population.

Difficulties in Establishing the Adverse Effects of ß-Casomorphin-7 Released from ß-Casein Variants-A Review.

ß-Casomorphin-7 (BCM-7) is a peptide released through the proteolysis of ß-casein (ß-CN), which is considered a bioactive peptide displaying evidence of promoting the binding and activation of the µ-opioid receptor located in various body parts, such as the gastrointestinal tract, the immune system and potentially the central nervous system. The possible effects of BCM-7 on health are a theme rising in popularity due to evidence found in several studies on the modulation of gastrointestinal proinflammatory responses that can trigger digestive symptoms, such as abdominal discomfort. With the advancement of studies, the hypothesis that there is a correlation of the possible effects of BCM-7 with the microbiota-gut-brain axis has been established. However, some studies have suggested the possibility that these adverse effects are restricted to a portion of the population, and the topic is controversial due to the small number of in vivo studies, which makes it difficult to obtain more conclusive results. In addition, a threshold of exposure to BCM-7 has not yet been established to clarify the potential of this peptide to trigger physiological responses at gastrointestinal and systemic levels. The proportion of the population that can be considered more susceptible to the effects of BCM-7 are evidenced in the literature review. The challenges of establishing the adverse effects of BCM-7 are discussed, including the importance of quantifying the BCM-7 release in the different ß-CN genotypes. In summary, the reviewed literature provides plausible indications of the hypothesis of a relationship between ß-CN A1/BCM-7 and adverse health effects; however, there is need for further, especially in vivo studies, to better understand and confirm the physiological effects of this peptide.

Voluntary Hydration with Skimmed Lactose-Free Milk during Exercise in the Heat: Exploring Effectiveness and Tolerance.

Replacement of fluid losses (dehydration) during sports activities in the heat has been investigated with different beverages. Bovine milk has been recommended for post-exercise rehydration, but its use during exercise may provoke gastrointestinal disorders. This study compared voluntary fluid intake, hydration, and incidence and severity of gastrointestinal (GI) disorders during exercise in the heat under three conditions: no drink (ND), water (W), and skimmed lactose-free milk (SM). Sixteen physically active university students exercised at 32 °C and 70% RH for 90 min at 60-75% HRmax while drinking W or SM ad libitum, or ND assigned at random. A questionnaire explored possible GI disorders. Ad libitum intake was higher (p < 0.05) for water (1206.2 mL) than milk (918.8 mL). Dehydration showed significant differences for SM versus W and ND (W = 0.28% BM; SM = -0.07% BM; ND = 1.38% BM, p < 0.05). Urine volume was significantly higher (p < 0.05) in the W condition (W = 220.4 mL; SM = 81.3 mL; ND = 86.1 mL). Thick saliva, belching, and abdominal pain were higher for SM, but scores were low. Skimmed lactose-free milk is a suitable, effective alternative to be consumed as a hydration beverage during moderate-intensity cycling in the heat for 90 min.

Effect of Agave Fructans on Changes in Chemistry, Morphology and Composition in the Biomass Growth of Milk Kefir Grains.

Prebiotic effects have been attributed to agave fructans through bacterial and yeast fermentations, but there are few reports on their use as raw materials of a carbon source. Kefir milk is a fermented drink with lactic acid bacteria and yeast that coexist in a symbiotic association. During fermentation, these microorganisms mainly consume lactose and produce a polymeric matrix called kefiran, which is an exopolysaccharide composed mainly of water-soluble glucogalactan, suitable for the development of bio-degradable films. Using the biomass of microorganisms and proteins together can be a sustainable and innovative source of biopolymers. In this investigation, the effects of lactose-free milk as a culture medium and the addition of other carbon sources (dextrose, fructose, galactose, lactose, inulin and fructans) in concentrations of 2, 4 and 6% w/w, coupled with initial parameters such as temperature (20, 25 and 30 °C), % of starter inoculum (2, 5 and 10% w/w) was evaluated. The method of response surface analysis was performed to determine the optimum biomass production conditions at the start of the experiment. The response surface method showed that a 2% inoculum and a temperature of 25 °C were the best parameters for fermentation. The addition of 6% w/w agave fructans in the culture medium favored the growth of biomass (75.94%) with respect to the lactose-free culture medium. An increase in fat (3.76%), ash (5.57%) and protein (7.12%) content was observed when adding agave fructans. There was an important change in the diversity of microorganisms with an absence of lactose. These compounds have the potential to be used as a carbon source in a medium culture to increase kefir granule biomass. There was an important change in the diversity of microorganisms with an absence of lactose, where the applied image digital analysis led to the identification of the morphological changes in the kefir granules through modification of the profile of such microorganisms.

Seasonal quality of milk from Creole grazing goats, stabled Saanen and French-Alpine goats / Calidad estacional de leche de cabras Criollas en pastoreo vs cabras estabuladas Saanen y Alpina-Francesa / Qualidade sazonal do leite de cabras Crioulas em pastagem vs cabras estábuladas Saanen e Franco-alpina

Abstract Background: Goat milk production has been increasing in northern Mexico; however, there is little information available about its quality. Objective: To compare goat milk quality during three seasons among creole goats in a traditional grazing system and stabled Saanen and French-Alpine goats. Methods: An experiment was performed in a completely randomized nested design with seasonality factor (fixed effects) with three levels (rain, transition, and drought) and breed factor (fixed effect with three levels (Creole, Saanen and French-Alpine)) nested within the season-of-the-year factor. Fifteen goats were randomly selected: five Creole in the extensive grazing system, five stabled Saanen, and five stabled French-Alpine. The factors assessed in milk were fat content, non-fat solids, protein, lactose, salts, density, freezing point, conductivity, and pH (using a Lactoscan® device). Results: With respect to season for all the breeds: fat, non-fat solids, protein, lactose, and salt contents were higher (p≤0.05) for goats sampled in the rainy season, followed by drought and transition seasons, respectively. The variables related to milk quality by breed and season showed that milk fat content of creole goats was greater (p≤0.05) in the drought season; salt and non-fat content (p≤0.05) in the three seasons of the year; density and protein increased (p≤0.05) in the transition and rainy seasons, while lactose was greater (p≤0.05) in drought and rainy seasons; protein was greater (p≤0.05) in transition and rainy seasons; and the freezing point was lower (p≤0.05) in the transition season. Regarding conductivity, French-Alpine milk was greater (p≤0.05) in the drought season. Conclusion: Season of the year significantly affects milk quality, with increased quality during the rainy season in creole grazing goats.

Resumen Antecedentes: La producción de leche de cabra se ha venido incrementando en el norte de México,; sin embargo, existe poca información sobre su calidad. Objetivo: Comparar la calidad de la leche de cabra durante tres épocas del año para cabras criollas en pastoreo y cabras puras de dos razas diferentes bajo estabulación. Métodos: El experimento se realizó mediante un diseño anidado completamente aleatorizado, con el factor época del año (efecto fijo) con tres niveles (lluvia, transición y sequia) y el factor raza (efecto fijo) con tres niveles (Criolla, Saanen y Alpino-Francesa) anidado dentro del factor época del año. En el sistema de pastoreo extensivo se eligieron al azar cinco cabras de raza Criolla, en el sistema estabulado se eligieron al azar cinco cabras de la raza Saanen y cinco de la raza Alpino-Francesa. La variables medidas fueron: contenido de grasa, sólidos no grasos, proteína, lactosa, sales, densidad, punto de congelación, conductividad y pH (utilizando el dispositivo Lactoscan®). Resultados: Respecto a la temporada del año para las tres razas se encontró que los contenidos en leche de grasa, sólidos no grasos, proteína, lactosa y sales fueron mayores (p≤0,05) en la época de lluvias, seguido por la leche muestreada en sequía y transición. Las variables por raza y temporada del año arrojaron que la grasa fue mayor (p≤0,05) en cabras criollas en sequía, solidos no grasos fue mayor (p≤0,05) en las tres temporadas del año en cabras criollas, la densidad aumentó (p≤0,05) en cabras criollas en transición y lluvia. La proteína fue mayor (p≤0,05) en cabras criollas en transición y lluvia, mientras que la lactosa fue mayor (p≤0,05) en cabras criollas en sequía y lluvia. El menor punto de congelación (p≤0,05) se presentó en cabras criollas en transición, la conductividad fue mayor (p≤0,05) en cabras Alpina-Francesa en sequía y la concentración de sales fue mayor (p ≤0,05) en cabras criollas durante las tres temporadas. Conclusión: Existe un marcado efecto de la temporada sobre la calidad de la leche, siendo la epoca de lluvias cuando se presenta mejor calidad en cabras criollas en pastoreo.

Resumo Antecedentes: No norte do México a produção de leite de cabra aumentou; no entanto, a qualidade do leite é desconhecida. Objetivo: Comparar a qualidade do leite de cabra durante três estações do ano com cabras crioulas em sistema de pastoreio tradicional e cabras Saanen e franco-alpinas em estábulos. Métodos: O experimento foi realizado através de um delineamento completamente casualizado com o fator de sazonalidade (efeitos fixos) com três níveis (chuva, transição e seca) e fator de raça (efeito fixo com três níveis (crioulo, Saanen e franco-alpino) aninhados dentro do fator estação do ano quinze cabras foram selecionadas aleatoriamente, cinco no sistema de pastejo extensivo, cinco Saanen e cinco Franco-Alpino no sistema estábulo. Os fatores avaliados no leite foram teor de gordura, sólidos desengordurados, proteína, lactose, sais, densidade, ponto de congelamento, condutividade e pH pelo aparelho Lactoscan®. Resultados: Em relação às estações do ano, nas três raças, mostrou que os teores de gordura, sólidos desengordurados, proteínas, lactose e sal foram maiores (p≤0,05) nas cabras cujo leite foi amostrado na ocasião um de chuvas seguido por aquele amostrado nas estações de seca e transição, respectivamente. As variáveis relacionadas à qualidade do leite de cabra por raça e ano, mostraram que nas cabras crioulas o teor de gordura foi maior (p≤0,05) na estação seca; teor de sal e gordura (p≤0,05) nas três estações do ano; a densidade aumentou significativamente e o teor de proteína foi maior (p≤0,05) nas estações de transição e chuvas, enquanto o valor de lactose foi maior (p≤0,05) nas estações seca e chuvosa; o teor de proteína foi maior (p≤0,05) nas estações de transição e chuvas; e o ponto de congelamento foi menor (p≤0,05) na estação de transição. Em relação à condutividade, o leite de cabra franco-alpino apresentou valor significativamente superior (p≤0,05) na seca. Conclusão: A qualidade do leite tem um efeito significativo por estação, sendo que a estação chuvosa aumentou a qualidade do leite em cabras crioulas em pastejo.

[Prevalence of cow´s milk allergy and lactose intolerance among Mexican late adolescents]. / Prevalencia de alergia a la leche de vaca e intolerancia a la lactosa en adolescentes tardíos mexicanos.

OBJECTIVE: To determine the prevalence of cow´s milk allergy and lactose intolerance in a sample of late adolescents. METHODS: Through a population-based study, data corresponding to students with aged 15 to 18 years were analyzed. RESULTS: A total of 1992 adolescents was analized. The prevalence of cow´s milk allergy was 1.4% (95% CI: 0.2% to 0.8%) and the prevalence of lactose intolerance was 0.5% (95% CI: 0.2% to 0.8%). Adolescents with cow´s milk allergy had fewer gastrointestinal symptoms (p = 0.036), but more skin (p < 0.001) and respiratory (p = 0.028) ailments than adolescents with lactose intolerance. CONCLUSIONS: The manifestations associated with cow's milk consumption in late adolescents seem to correspond mainly to cow´s milk allergy than to lactose intolerance.

OBJECTIVO: Determinar la prevalencia de alergia a la proteína de la leche de vaca e intolerancia a la lactosa en una muestra de adolescentes tardíos. MÉTODOS: Estudio transversal con base poblacional, en el que se analizaron los datos de pacientes adolescentes de 15 a 18 años. RESULTADOS: Se registraron 1992 estudiantes. La prevalencia de alergia a la proteína de la leche de vaca fue de 1.4% (IC95%: 0.9% a 2.0%) y de intolerancia a la lactosa de 0.5% (IC95%: 0.2% a 0.8%). Los adolescentes con alergia a la proteína de la leche de vaca tuvieron menos síntomas gastrointestinales (p = 0.036), pero más molestias cutáneas (p < 0.001) y respiratorias (p = 0.028) que los adolescentes con intolerancia a la lactosa. CONCLUSIONES: Las manifestaciones asociadas con el consumo de leche de vaca en adolescentes tardíos corresponden, principalmente, a la alergia de la proteína de la leche de vaca que a la intolerancia a la lactosa.

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor.

Agaricus bisporus mushroom biomass contains a lectin, ABL, with remarkable specificity for lactose biorecognition; in this work, this feature was explored to develop a photoelectrochemical biosensor. The high lectin activity found in saline extracts of this macrofungus (640 HU mL-1), even at critical pH values (4-10) and temperatures (20-100 °C), allowed its direct use as an ABL source. Theoretical and experimental evidence revealed favorable electrostatic and biocompatible conditions to immobilize ABL on a poly(methylene blue)/fluorine-doped tin oxide-coated glass platform, giving rise to the ABL/PMB/FTO biosensor. The conducting polymer added further photoactivity to the device, allowing the identification of lectin-carbohydrate interactions with even greater sensitivity. The dose-response curves studied by electrochemical impedance spectroscopy showed a sigmoidal profile that was well-fitted by Hill's equation, expanding the working dynamic range (15-540 nmol L-1 lactose; 20.2 pmol L-1 detection limit) and avoiding undesirable sample dilution or preconcentration procedures. Under the optimized photoelectrochemical conditions, the ABL/PMB/FTO biosensor showed remarkable signal stability, accuracy, specificity, and selectivity to analyze lactose in commercial food products. This research raises interest in ABL-based biosensors and the added value of the crude Agaricus bisporus extract toward the development of greener and more sustainable biotechnological approaches.

Determination of the lactose content in low-lactose milk using Fourier-transform infrared spectroscopy (FTIR) and convolutional neural network.

Demand for low lactose milk and milk products has been increasing worldwide due to the high number of people with lactose intolerance. These low lactose dairy foods require fast, low-cost and efficient methods for sugar quantification. However, available methods do not meet all these requirements. In this work, we propose the association of FTIR (Fourier Transform Infrared) spectroscopy with artificial intelligence to identify and quantify residual lactose and other sugars in milk. Convolutional neural networks (CNN) were built from the infrared spectra without preprocessing the data using hyperparameter adjustment and saliency map. For the quantitative prediction of the sugars in milk, a regression model was proposed, while for the qualitative assessment, a classification model was used. Raw, pasteurized and ultra-high temperature (UHT) milk was added with lactose, glucose, and galactose in six concentrations (0.1-7.0 mg mL-1) and, in total, 432 samples were submitted to convolutional neural network. Accuracy, precision, sensitivity, specificity, root mean square error, mean square error, mean absolute error, and coefficient of determination (R2) were used as evaluation parameters. The algorithms indicated a predictive capacity (accuracy) above 95% for classification, and R2 of 81%, 86%, and 92% for respectively, lactose, glucose, and galactose quantification. Our results showed that the association of FTIR spectra with artificial intelligence tools, such as CNN, is an efficient, quick, and low-cost methodology for quantifying lactose and other sugars in milk.

Associations of Secretory Activation Breast Milk Biomarkers with Breastfeeding Outcome Measures.

OBJECTIVE: The objective of this study was to explore associations between day 10 postpartum (D10) secretory activation biomarkers and the breastfeeding outcome measures. STUDY DESIGN: This prospective longitudinal descriptive study collected antepartum, D10, and day 60 postpartum (D60) questionnaire data and D10 milk samples. Protein, lactose, and citrate were analyzed with enzymatic spectrophotometric assays. Sodium and potassium were analyzed with inductively coupled plasma optical emission spectrophotometry. Group comparison data were analyzed using χ2, Fisher exact, and independent sample t tests, as appropriate, using SPSS for Mac (version 28). RESULTS: Participants (n = 92) provided a D10 breastmilk sample and completed D10 questionnaires, and 83 completed D60 questionnaires. Participants with D10 impaired secretory activation sodium (>23.0 mM) were more likely to report D10 perceived insufficient milk supply, χ2 = 7.002, P < .05; and less D10 feeding/pumping frequency a day, P < .05; and partial breastfeeding at D60, P < .05. Additionally, participants with D10 impaired secretory activation sodium-to-potassium ratio (sodium: potassium) > 0.8 were more likely to partially breastfeed at D60, P < .05. CONCLUSION: Elevated milk sodium and sodium: potassium are biomarkers related to variables indicative of low milk supply. Therefore, immediate milk testing can be useful in identifying lactation compromise and improving breastfeeding duration. Because breastfeeding affords maternal and infant health benefits, clinicians should identify ways to measure lactation compromise in conjunction with an examination and clinical history to provide early interventions to increase breastfeeding duration and exclusivity.

Retrospective evalution of non-fatty solids in samples of raw milk in the state of Rio Grande do Sul according to season, Brazil / Estudo retrospectivo dos sólidos não gordurosos em amostras de leite cru no estado do Rio Grande do Sul de acordo com as estações do ano

ABSTRACT: The present study described the chemical composition and somatic cell score (SCS) of samples of refrigerated raw milk collected from commercial farms in the state of Rio Grande do Sul in order to better understand the behavior of constituents present in non-fatty solids (NFS) in milk according to the season of the year. Means were used to describe statistical data. To estimate the probability of NFS levels meeting IN 76 (BRAZIL, 2018), binary logistic regression was used. It was reported that 18.2% (233.817) of analytical results showed NFS below 8.4%, representing the minimum required by IN 76. The highest average NFS level observed in the five-year period was registered in the micro-region of Passo Fundo (8.70%) in winter. The microregion with the lowest results was Porto Alegre (8.53%); however, it still demonstrated levels within the limits established by IN 76. The study indicates that milk constituents show differences between seasons. In autumn and winter, the constituents remained equal to or higher than those required by current legislation, while spring and summer were the periods with the lowest NFS values. The SCS was also influenced by the seasons, with the highest rates in spring, summer, and autumn.

RESUMO: O presente estudo teve como objetivo descrever os resultados de composição química e escore de células somáticas (ECS) de amostras de leite cru refrigerado coletado em fazendas comerciais no estado do Rio Grande do Sul, para melhor entendimento do comportamento dos constituintes presentes nos sólidos não gordurosos (SNG) no leite de acordo com as estações do ano. As médias foram estudadas para descrever as estatísticas dos dados. Para estimar a probabilidade de os teores de SNG atenderem à IN 76 de 2018, foi utilizada a regressão logística binária. Foi constatado que 18,2% (233.817) dos resultados analíticos apresentaram SNG abaixo de 8,4%, que representa o mínimo exigido pela IN 76 (BRASIL, 2018). A maior média de SNG observada no período de cinco anos foi registrada na microrregião de Passo Fundo (8,70%), no inverno. A microrregião com menores resultados foi a de Porto Alegre (8,53%), no entanto com teores dentro do estabelecido pela IN 76/2018. O estudo demonstrou que os constituintes do leite apresentaram diferenças entre as estações do ano. O outono e inverno foram os períodos em que os constituintes se mantiveram iguais ou superiores aos exigidos pela legislação vigente, enquanto que a primavera e o verão foram os períodos com os menores valores de SNG. O ECS também foi influenciado pelas estações do ano. Na primavera, verão e outono ocorreram os maiores índices.

Retrospective evalution of non-fatty solids in samples of raw milk in the state of Rio Grande do Sul according to season, Brazil / Estudo retrospectivo dos sólidos não gordurosos em amostras de leite cru no estado do Rio Grande do Sul de acordo com as estações do ano

The present study described the chemical composition and somatic cell score (SCS) of samples of refrigerated raw milk collected from commercial farms in the state of Rio Grande do Sul in order to better understand the behavior of constituents present in non-fatty solids (NFS) in milk according to the season of the year. Means were used to describe statistical data. To estimate the probability of NFS levels meeting IN 76 (BRAZIL, 2018), binary logistic regression was used. It was reported that 18.2% (233.817) of analytical results showed NFS below 8.4%, representing the minimum required by IN 76. The highest average NFS level observed in the five-year period was registered in the micro-region of Passo Fundo (8.70%) in winter. The microregion with the lowest results was Porto Alegre (8.53%); however, it still demonstrated levels within the limits established by IN 76. The study indicates that milk constituents show differences between seasons. In autumn and winter, the constituents remained equal to or higher than those required by current legislation, while spring and summer were the periods with the lowest NFS values. The SCS was also influenced by the seasons, with the highest rates in spring, summer, and autumn.

O presente estudo teve como objetivo descrever os resultados de composição química e escore de células somáticas (ECS) de amostras de leite cru refrigerado coletado em fazendas comerciais no estado do Rio Grande do Sul, para melhor entendimento do comportamento dos constituintes presentes nos sólidos não gordurosos (SNG) no leite de acordo com as estações do ano. As médias foram estudadas para descrever as estatísticas dos dados. Para estimar a probabilidade de os teores de SNG atenderem à IN 76 de 2018, foi utilizada a regressão logística binária. Foi constatado que 18,2% (233.817) dos resultados analíticos apresentaram SNG abaixo de 8,4%, que representa o mínimo exigido pela IN 76 (BRASIL, 2018). A maior média de SNG observada no período de cinco anos foi registrada na microrregião de Passo Fundo (8,70%), no inverno. A microrregião com menores resultados foi a de Porto Alegre (8,53%), no entanto com teores dentro do estabelecido pela IN 76/2018. O estudo demonstrou que os constituintes do leite apresentaram diferenças entre as estações do ano. O outono e inverno foram os períodos em que os constituintes se mantiveram iguais ou superiores aos exigidos pela legislação vigente, enquanto que a primavera e o verão foram os períodos com os menores valores de SNG. O ECS também foi influenciado pelas estações do ano. Na primavera, verão e outono ocorreram os maiores índices.

Virulence Profile, Antibiotic Resistance, and Phylogenetic Relationships among Escherichia coli Strains Isolated from the Feces and Urine of Hospitalized Patients.

Extra-intestinal pathogenic Escherichia coli (ExPEC) may inhabit the human gut microbiota without causing disease. However, if they reach extra-intestinal sites, common cystitis to bloodstream infections may occur, putting patients at risk. To examine the human gut as a source of endogenous infections, we evaluated the E. coli clonal diversity of 18 inpatients' guts and their relationship with strains isolated from urinary tract infection (UTI) in the same hospital. Random amplified polymorphic DNA evaluated the clonal diversity, and the antimicrobial susceptibility was determined by disk diffusion. One isolate of each clone detected was sequenced, and their virulome and resistome were determined. Overall, 177 isolates were screened, among which 32 clones were identified (mean of two clones per patient), with ExPEC strains found in over 75% of the inpatients' guts. Endogenous infection was confirmed in 75% of the cases. ST10, ST59, ST69, ST131, and ST1193 clones and critical mobile drug-resistance encoding genes (blaCTX-M-15, blaOXA-1, blaDHA-1, aac(6')-lb-cr, mcr-1.26, qnrB4, and qnrB19) were identified in the gut of inpatients. The genomic analysis highlighted the diversity of the fecal strains, colonization by lactose-negative E. coli, the high frequency of ExPEC in the gut of inpatients without infections, and the presence of ß-lactamase producing E. coli in the gut of inpatients regardless of the previous antibiotics' usage. Considering that we found more than one ExPEC clone in the gut of several inpatients, surveillance of inpatients' fecal pathogens may prevent UTI caused by E. coli in the hospital and dissemination of risk clones.