Non-destructive environmental safety assessment of threatened and endangered plants in weed biological control.

Assessing the risk of nontarget attack (NTA) for federally listed threatened and endangered (T&E) plant species confamilial to invasive plants targeted for classical biological control, is one of the most important objectives of pre-release environmental safety assessments in the United States. However, evaluating potential NTA on T&E species is often complicated by restrictive agency requirements for obtaining propagules, or the ability to propagate plants and rear agents to the appropriate phenostages synchronously for testing, or both. Here, we assessed whether plant cues associated with a host recognition can be used for testing the attractiveness of four T&E and one rare single population plant species non-destructively for a candidate biocontrol agent. We used the seed-feeding weevil, Mogulones borraginis, a candidate for the biological control of the invasive plant, Cynoglossum officinale (Boraginaceae) as the study system. We collected olfactory and visual cues in the form of flowering sprigs from T&E plant species confamilial to the invasive plant in a non-destructive manner and used them to measure behavioral responses and searching time of weevils. Female weevils preferred C. officinale to all tested plant species in dual-choice bioassays using either olfactory or visual cues in a modified y-tube device. Furthermore, female weevils were repelled by the combined olfactory and visual cues from all tested T&E plant species in a dual-choice test against controls (e.g., purified air in an empty arm), indicating that it would be extremely unlikely for the weevil to attack any of these species upon release in the United States. Principal component analysis based on 61 volatile organic compounds effectively separated the five confamilial plant species and C. officinale, corroborating the results of behavioral bioassays. We conclude that studies on pre-alighting host selection behavior and the underlying physiological mechanisms of how organisms select host plants they exploit can aid in environmental safety testing of weed biological control agents.

Prophylactic Common Peroneal Nerve Decompression Avoids Nerve Palsy in Total Knee Arthroplasty for Severe Fixed Valgus Deformity: A Report of Four Cases and Review of Literature.

Common peroneal nerve (CPN) injury is a serious complication following total knee arthroplasty (TKA). We aim to report four patients (five knees) who underwent prophylactic peroneal nerve decompression for severe rigid valgus deformity with or without associated fixed flexion deformity that was not correctable under anaesthesia. The preoperative deformity of 31.1° valgus by femorotibial angle (range 22.6-37.9°) improved to 7.1° valgus (range 4.3-9.1°) postoperatively (p < 0.05). For two knees, varus-valgus constrained was used due to medial laxity and the other three had posterior-stabilised prosthesis. All four patients had normal motor or sensory nerve function of the CPN nerve postoperatively. There was a significant improvement in the functional outcome by knee society score and knee society functional score from 17.8 ± 6.8, 25 ± 16.2 to 84 ± 8.7, 83 ± 10.3, respectively (p < 0.05). No complications were noted in the mean follow-up of 1.2 years. Prophylactic peroneal nerve decompression allows safe, adequate and optimal lateral soft-tissue release. It is effective in preventing common peroneal nerve palsy in high-risk patients like severe valgus and flexion deformity during total knee arthroplasty.

When a plant invader meets its old enemy abroad: what can be learnt from accidental introductions of biological control agents.

Accidental introductions of biological weed control (BWC) agents (i) offer opportunities to assess host use of agents with a potentially broader fundamental host-range than those approved for field release directly in target areas; (ii) urge national authorities to rapidly respond as they may threaten native species or crops, and by this (iii) help advancing post-release studies, a neglected aspect of BWC. Through detailed insights gained from studying the recent accidental introduction of the ragweed leaf beetle Ophraella communa into Europe, we derive suggestions for overcoming barriers to adoption of BWC by re-evaluating the predictive power of pre-release studies and, thus, the presently strict criteria for deciding upon their release that might exclude safe and efficient agents. By using the allergenic weed Ambrosia artemisiifolia and the accidentally introduced BWC agent O. communa as study system, we also hope to raise the awareness of authorities to consider biological control more prominently as a key approach for pest management in the 'One Health' concept, which aims to sustainably balance and optimize the health of people, animals, plants and ecosystems. © 2023 Society of Chemical Industry.

Successful acclimatization of mandrills (Mandrillus sphinx) translocated to Conkouati-Douli National Park, Republic of Congo, as measured by fecal glucocorticoid metabolites.

Translocation and reintroduction are common tools in conservation management and can be very successful. However, translocation can be stressful for the animals involved, and stress is implicated as a major cause of failure in release programs. Conservation managers should therefore seek to understand how the stages of translocation impact stress physiology in the animals involved. We quantified fecal glucocorticoid metabolites (fGCMs) as a noninvasive measure of response to potential stressors during a translocation of 15 mandrills (Mandrillus sphinx) into Conkouati-Douli National Park, Republic of Congo. The mandrills were initially housed in a sanctuary, transferred to a pre-release enclosure in the National Park and then released into the forest. We collected repeated fecal samples (n = 1101) from known individuals and quantified fGCMs using a previously validated enzyme immunoassay. Transfer from the sanctuary to the pre-release enclosure correlated with a significant 1.93-fold increase in fGCMs, suggesting that transfer was a stressor for the mandrills. fGCM values decreased over time in the pre-release enclosure, suggesting that the mandrills recovered from the transfer and acclimatized to the enclosure. Release to the forest was not linked to a significant increase in fGCMs over the final values in the enclosure. Following release, fGCMs continued to decrease, fell below sanctuary values after just over a month and were about half the sanctuary values after 1 year. Overall, our results suggest that the translocation, although initially presenting a physiological challenge to the animals, was not detrimental to the well-being of the animals over the timescale of the study and, in fact, may have been beneficial. Our findings show the value of non-invasive physiology in monitoring, evaluating and designing wildlife translocations and, ultimately, contributing to their success.

Spatio-Temporal Modelling Informing Wolbachia Replacement Releases in a Low Rainfall Climate.

Releases of Aedes aegypti carrying Wolbachia bacteria are known to suppress arbovirus transmission and reduce the incidence of vector-borne diseases. In planning for Wolbachia releases in the arid environment of Jeddah, Saudi Arabia, we collected entomological data with ovitraps across a 7-month period in four locations. Herein, we show that mosquito presence in basements does not differ from that of non-basement areas of buildings. In modelling mosquito presence across the study sites, we found the spatial structure to be statistically significant in one of the four sites, while a significant spatial structure was found for egg production data across three of the four sites. The length scales of the spatial covariance functions fitted to the egg production data ranged from 143 m to 574 m, indicating that high productivity regions can be extensive in size. Rank-correlation analyses indicated that mosquito presence tended to persist from the dry to wet season, but that egg production ranks at locations could reverse. The data suggest that, in Jeddah, the quality of the local environment for breeding can vary over time. The data support the feasibility of dry season releases but with release numbers needing to be flexible depending on local rates of invasion.

Common peroneal nerve 'pre-release' in total knee arthroplasty for severe valgus deformities.

BACKGROUND: Common peroneal nerve (CPN) palsy is a devastating complication that follows total knee arthroplasty (TKA). However, there are only a few studies on concrete measures for protecting the CPN in patients. This study aimed to put forward the CPN 'pre-release' method to protect the nerve. METHODS: A prospective study was conducted on 30 patients (34 knees) with severe valgus knees who underwent CPN pre-release. This was a two-incision approach, and required a separate dissection of about three centimeters from the TKA operation. Clinical measurements including pre- and postoperative motor and sensory nerve function of CPN, radiological evaluation, complications, and the revised data were analyzed and compared. RESULTS: The average preoperative femorotibial angle was 31.3 ± 8.0°. All patients had completely normal motor (grade 5) and sensory nerve function of CPN postoperatively, and there was no transient or late-onset CPN palsy. Patients had a routine rehabilitation with full weight bearing after recovery from anesthesia, including the knees with unconstrained extension/flexion motion. During the last follow-up visit, the visual analog scale, Knee Society Score, Hospital for Special Surgery knee-rating scale, and range of motion were 2.06 ± 1.13, 92.18 ± 5.57, 90.18 ± 3.70, and 115.59 ± 7.76°, respectively. There were no revisions for instability and recurrent valgus deformities during follow-up. Also, the femorotibial angle, hip-knee-ankle angle, condylar-hip angle, and plateau-ankle angle were 4.9 ± 2.0°, 179.09 ± 3.21°, 89.97 ± 2.41°, and 90.53 ± 1.26°, respectively. CONCLUSIONS: The CPN pre-release for severe valgus knees is an effective method for nerve protection, achieving an adequate and safe release of lateral soft tissue, and providing immediate and early functional rehabilitation with decreasing constrained implant.

Raspberry ketone supplement promotes early sexual maturation in male Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

BACKGROUND: Raspberry ketone (RK) is highly attractive to sexually mature, but not immature, males of many Bactrocera species, including Queensland fruit fly ('Qfly', Bactrocera tryoni), and acts as a metabolic enhancer in a wide diversity of animals. We considered the possibility that, as a metabolic enhancer, RK in adult diet might accelerate sexual maturation of male Qflies. RESULTS: Recently emerged adult Qfly males (0-24 h old) were exposed to RK-treated food for 48 h and were then provided only sugar and water. Four doses of RK (1.25, 2.5, 3.75 and 5%) along with control (0%) were tested with two types of food: sugar alone and sugar mixed with yeast hydrolysate (3:1). For flies tested when 4-10 days old all RK doses increased mating probability of flies fed sugar mixed with yeast hydrolysate but did not show any effect on mating probability of flies fed only sugar. No effects of RK were found for flies tested when 10-30 days old for either diet group. There was no evidence that RK affected longevity at any of the doses tested. CONCLUSION: Feeding of RK together with yeast hydrolysate to immature Qfly increases mating propensity at young ages and accordingly shows significant potential as a pre-release supplement that might increase the proportion of released flies that attain sexual maturation in Sterile Insect Technique programmes. © 2017 Society of Chemical Industry.