Evaluating the reliability of DNA Barcoding for Central American Pacific shallow water echinoderms identification: a molecular taxonomy and database accuracy analysis / Evaluando la fiabilidad del Código de Barras de ADN para la identificación de equinodermos en aguas poco profundas del Pacífico de América Central: un análisis de taxonomía molecular y precisión de bases de datos

Abstract Introduction: Molecular divergence thresholds have been proposed to distinguish recently separated evolutive units, often displaying more accurate putative species assignments in taxonomic research compared to traditional morphological approaches. This makes DNA barcoding an attractive identification tool for a variety of marine invertebrates, especially for cryptic species complexes. Although GenBank and the Barcode of Life Data System (BOLD) are the major sequence repositories worldwide, very few have tested their performance in the identification of echinoderm sequences. Objective: We use COI echinoderm sequences from local samples and the molecular identification platforms from GenBank and BOLD, in order to test their accuracy and reliability in the DNA barcoding identification for Central American shallow water echinoderms, at genus and species level. Methods: We conducted sampling, tissue extraction, COI amplification, sequencing, and taxonomic identification for 475 specimens. The 348 obtained sequences were individually enquired with BLAST in GenBank as well as using the Identification System (IDS) in BOLD. Query sequences were classified depending on the best match result. McNemar's chi-squared, Kruskal-Wallis's and Mann-Whitney's U tests were performed to prove differences between the results from both databases. Additionally, we recorded an updated list of species reported for the shallow waters of the Central American Pacific. Results: We found 324 echinoderm species reported for Central American Pacific shallow waters. Only 118 and 110 were present in GenBank and BOLD databases respectively. We proposed 325 solved morphology-based identities and 21 provisional identifications in 50 putative taxa. GenBank retrieved 348 molecular-based identifications in 58 species, including twelve provisional identifications in tree taxa. BOLD recovered 170 COI identifications in 23 species with one provisional identification. Nevertheless, 178 sequences retrieved unmatched terms (in 34 morphology-based taxa). Only 86 sequences (25 %) were retrieved as correct identifications and 128 (37 %) as identification errors in both platforms. We include 84 sequences for eleven species not represented in GenBank and 65 sequences for ten species in BOLD Echinoderm COI databases. The identification accuracy using BLAST (175 correct and 152 incorrect identifications) was greater than with IDS engine (110 correct and 218 identification errors), therefore GenBank outperforms BOLD (Kruskal-Wallis = 41.625, df = 1, p < 0.001). Conclusions: Additional echinoderm sample references are needed to improve the utility of the evaluated DNA barcoding identification tools. Identification discordances in both databases may obey specific parameters used in each search algorithm engine and the available sequences. We recommend the use of barcoding as a complementary identification source for Central American Pacific shallow water echinoderm species.

Resumen Introducción: Se han propuesto los umbrales de divergencia molecular para distinguir unidades evolutivas recientemente separadas, que a menudo muestran asignaciones de especies putativas más precisas en la investigación taxonómica en comparación con los enfoques morfológicos tradicionales. Esto hace que los Códigos de Barras de ADN sean una herramienta de identificación atractiva para una variedad de invertebrados marinos, especialmente para complejos de especies crípticas. Aunque GenBank y Barcode of Life Data System (BOLD) son los principales repositorios de secuencias en todo el mundo, muy pocos han probado su desempeño en la identificación de secuencias de equinodermos. Objetivo: Utilizamos secuencias de equinodermos COI de muestras locales y las plataformas de identificación molecular de GenBank y BOLD, para probar su precisión y confiabilidad en la implementación de códigos de barras de ADN para equinodermos de aguas someras de Centroamérica, a nivel de género y especie. Métodos: Realizamos muestreo, extracción de tejido, amplificación de COI, secuenciación e identificación taxonómica de 475 especímenes. Las 348 secuencias obtenidas fueron consultadas individualmente con BLAST en GenBank así como utilizando el Sistema de Identificación (IDS) en BOLD. Las secuencias consultadas se clasificaron según el mejor resultado de coincidencia. Se realizaron las pruebas chi-cuadrado de McNemar, Kruskal-Wallis y U de Mann-Whitney para comprobar diferencias entre los resultados de ambas bases de datos. Además, registramos una lista actualizada de especies reportadas para las aguas someras del Pacífico Centroamericano. Resultados: Encontramos 324 especies de equinodermos reportadas para aguas someras (< 200 m) del Pacífico centroamericano. Sólo 118 y 110 estaban presentes en las bases de datos GenBank y BOLD respectivamente. Propusimos 325 identidades resueltas basadas en morfología y 21 identificaciones provisionales en 50 taxones putativos. GenBank recuperó 348 identificaciones de base molecular en 58 especies, incluidas doce identificaciones provisionales en tres taxones. BOLD recuperó 170 identificaciones de COI en 23 especies con una identificación provisional. Sin embargo, 178 secuencias recuperaron términos no coincidentes (en 34 taxones basados en morfología). Sólo 86 secuencias (25 %) se recuperaron como identificaciones correctas y 128 (37 %) como errores de identificación en ambas plataformas. Incluimos 84 secuencias para once especies no representadas en GenBank y 65 secuencias para diez especies ausentes en las bases de datos BOLD Echinoderm COI. La precisión de la identificación usando BLAST (175 identificaciones correctas y 152 incorrectas) fue mayor que con el motor IDS (110 correctas y 218 errores de identificación), por lo tanto, GenBank supera a BOLD (Kruskal-Wallis = 41.625, df = 1, p < 0.001). Conclusiones: Se necesitan muestras adicionales de equinodermos de referencia para mejorar la utilidad de las herramientas de identificación de códigos de barras de ADN evaluadas. Las discordancias de identificación en ambas bases de datos pueden obedecer a parámetros específicos utilizados en cada algoritmo de búsqueda y a las secuencias disponibles. Recomendamos el uso de códigos de barras como fuente de identificación complementaria para las especies de equinodermos de aguas someras del Pacífico centroamericano.

Contribution to the knowledge about mammal collections in Brazil

We provide a brief introduction on the importance and use of scientific collections and a general overview of the articles that are part of the special issue of the Brazilian Journal of Mammalogy on Brazilian mammal collections. The 19 articles that comprise this issue refer to collections distributed across eight Brazilian states. Most of the collections hold specimens of living mammals, and two of them are paleontological collections. Two articles address specific orders of mammals, while the rest present data for all the orders. We hope that this issue represents a significant contribution to efforts to preserve Brazilian scientific collections, providing a more comprehensive view of their importance and the current and potential use of the specimens preserved therein.

Apresentamos uma breve introdução sobre a importância e uso de coleções científicas e um panorama geral dos artigos que fazem parte do número especial do Brazilian Journal of Mammalogy sobre coleções brasileiras de mamíferos. Os 19 artigos abrangem coleções distribuídas em oito estados da federação. Em sua maioria tratam de mamíferos viventes, sendo dois referentes a coleções paleontológicas. Dois artigos abordam ordens específicas de mamíferos e os demais apresentam dados para todas as ordens. Esperamos que este número represente uma contribuição significativa aos esforços para preservar as coleções científicas nacionais, fornecendo uma visão mais abrangente acerca das coleções e do uso atual e potencial dos espécimes nelas preservados.

Revisión histórica, distribución potencial y estado de conservación de la rata endémica de México Xenomys nelsoni (Rodentia: Cricetidae) / Historical review, potential distribution, and conservation of the Mexican endemic rat Xenomys nelsoni (Rodentia: Cricetidae)

Resumen Introducción: La Rata de Magdalena, Xenomys nelsoni, es un roedor endémico de México, de distribución restringida a las selvas bajas caducifolias densas, en una pequeña región de la costa del Pacífico mexicano. Es una especie poco conocida, catalogada como "En Peligro" de acuerdo con la Unión Internacional para la Conservación de la Naturaleza (IUCN). Este desconocimiento unido a la alta tasa de deforestación de su hábitat hace que su conservación sea prioritaria. Objetivo: Realizar un recuento histórico de los registros depositados en las colecciones científicas, generar mapas de distribución potencial y analizar el estado de conservación de la especie. Método: Los datos de ocurrencia de las especies se obtuvieron de la literatura y bases de datos digitales y se analizaron por décadas. Se utilizaron los programas GARP y MaxEnt para generar los modelos de nicho ecológico. La importancia de las variables en el modelo se estimó mediante un análisis Jackknife. Resultados: A lo largo de 129 años 19 recolectores registraron 69 ejemplares, de los cuales 65 están depositados en siete colecciones internacionales y una nacional. Aunque la especie sólo se ha recolectado en Jalisco y Colima, la distribución potencial de X. nelsoni incluye también el estado de Michoacán. De esta área estimada, sólo el 1.5 % se encuentra dentro de un Área Natural Protegida. Conclusiones: Los resultados de la distribución potencial podrían ser utilizados para verificar la presencia de la especie en lugares donde no ha sido recolectada como el norte de la Reserva de la Biosfera Chamela-Cuixmala y en algunas zonas de la provincia fisiográfica Costas del Sur en el estado de Michoacán. Es necesario incrementar los muestreos en regiones poco estudiadas predichas por el modelo y aumentar el área de protección.

Abstract Introduction: The Magdalena Rat, Xenomys nelsoni, is a rodent endemic to Mexico, whose distribution is restricted to dense tropical dry forests in a small region on the Pacific coast of Mexico. It is a poorly known species categorized as "Endangered" by the International Union for Conservation of Nature (IUCN). This lack of knowledge and the high rates of deforestation of its habitat makes its conservation a priority. Objective: To summarize the historical records deposited in scientific collections, to create potential distribution maps, and to analyze the conservation status of the species. Methods: We obtained species occurrence data from literature and digital databases, analyzing them by the decade. We used GARP and MaxEnt software to generate the ecological niche models. The importance of the variables in the model was estimated using the Jackknife technique. Results: Over 129 years, 19 collectors registered 69 specimens, of which 65 are deposited in one national and seven international collections. Although the species has only been collected in Jalisco and Colima, the potential distribution for X. nelsoni also includes the state of Michoacán. Of this estimated area, only 1.5 % is in a Protected Natural Area. Conclusions: The results of the potential distribution could be used to verify the presence of the species in places where it has not been collected, such as the northern part of the Chamela-Cuixmala Biosphere Reserve and in some areas of the physiographic province Costas del Sur in the state of Michoacán. It is needed to increase samplings in the least studied regions predicted by the model and expand the area of protection.

Citellinema (Nematoda: Heligmosomidae) from North America with descriptions of 2 new species from the red squirrel Tamiasciurus hudsonicus and 1 from the Canadian woodchuck, Marmota monax.

Citellinema Hall, 1918 includes 6 valid species of gastrointestinal nematodes of sciurids. Two species occur in the Palearctic and 4 in the Nearctic, 3 of which occur minimally across Colorado, Idaho and Oregon and 1, Citellinema bifurcatum, has a wide distribution across North America. Members of the genus are didelphic, possess a cephalic vesicle, a terminal spine-like process in females and feature robust spicules, consisting of a proximal end fused and semicylindrical shaft connected to a lamina supported by 2 terminal filiform processes. Typically, the size of the spicules is used to differentiate species. As part of the Beringian Coevolution Project, specimens provisionally identified as C. bifurcatum were collected through intensive field sampling of mammals and associated parasites from across localities spanning the Holarctic. These specimens revealed considerable genetic variability at both mitochondrial and nuclear loci, supporting the identification of deeply divergent clades. Examination of these new specimens, along with the holotypes of C. bifurcatum and Citellinema quadrivittati indicates that Citellinema monacis (previously synonymized with C. bifurcatum) should be resurrected and 3 additional species described. We suggest that the apparent bifurcated nature of the spicule should be considered a generic diagnostic trait, while the proportional length of the lamina relative to that of the spicule is used as a specific character. We demonstrate the critical need for continued inventory of often poorly known assemblages of hosts and parasites, contributing to a growing baseline of archival specimens, collections and information that make explorations of faunal structure and diversity possible.

Italian natural history museums need specimen digitization and much more: a reply to Benvenuti et al.

We reply to the comments made by Benvenuti et al. (2022) about our paper on the Italian natural history museums and scientific collections and the need of a centralized hub and repository. While agreeing that digitization is a useful tool to valorize each museum and collection, we still believe that the suggestion of a centralized hub is valid and necessary. This would largely help in boosting coordination among museums, sharing personnel and resources, and in providing a place to deposit scientific collections that do not fit the scope of smaller museums.

The Herbaria of the State of São Paulo / Os Herbários do Estado de São Paulo

Abstract This article compiles information on FAPESP's support for improving environmental conditions (temperature and relative humidity), expansion (acquisition of conventional and compacted cabinets) and conservation of botanical collections deposited in São Paulo's herbaria. Aspects related to the qualification of these collections and repatriation of data on Flora Paulista are also highlighted.

Resumo Neste trabalho são compiladas informações sobre o apoio da FAPESP à melhoria das condições ambientais (temperatura e umidade relativa), ampliação (aquisição de armários convencionais e compactados) e conservação das coleções botânicas depositadas nos herbários paulistas. Também são destacados, aspectos relacionados à qualificação dessas coleções e repatriação de dados sobre a Flora Paulista.

Las aves del departamento de Loreto, Perú / The birds from Department of Loreto, Peru

Resumen Presentamos una lista actualizada de las especies de aves del departamento de Loreto. La lista está basada en la revisión de ejemplares de colecciones científicas, registros históricos, publicaciones y observaciones de campo. Reportamos 1040 especies de aves para Loreto, 72 de ellas en alguna categoría de amenaza, 72 migratorias y 13 endémicas de Perú. Percnostola arenarum, Pithys castaneus y Polioptila clementsi son endémicas de Loreto. Las familias con mayor número de especies son Tyrannidae, Thamnophilidae y Thraupidae. La lista presentada tiene alta confiabilidad debido al respaldo de cerca del 81% de las especies en especímenes de colecciones científicas y apenas el 1.5% solo por reportes de avistamientos realizados por ornitólogos. Las especies de aves de Loreto representan alrededor del 55% del total reportado para el Perú y en Passeriformes el 52% de los estimado para la Amazonia. La distribución de los registros muestra que es necesario explorar áreas cercanas a las fronteras con Colombia y Brasil, así como las cordilleras que comparten con Amazonas y San Martín. En base a la distribución de la avifauna amenazada y endémica se propone priorizar el estudio y protección de áreas montanas y de bosques de arena blanca en particular.

Abstract We present an updated list of bird species from Department of Loreto. The list is based on the review of specimens from scientific collections, historical records, publications, and field observations. We report 1040 species of birds in Loreto, 72 in some threat category, 72 migratory and 13 endemics to Peru. Percnostola arenarum, Pithys castaneus and Polioptila clementsi are endemics to Loreto. The families with the highest numbers of species are Tyrannidae, Thamnophilidae and Thraupidae. The list presented is highly reliable due to the support of about 81% of the species on specimens from scientific collections and only 1.5% from sighting reports by ornithologists. Bird species from Loreto represent about 55% of the total reported for Peru and in Passeriformes 52% of those estimated for the Amazonia. The distribution of reports shows that it is necessary to explore areas close to the region's borders with Colombia and Brazil, as well as the mountain ranges shared with Amazonas and San Martin. Based on the distribution of threatened and endemic avifauna, we propose to prioritize the study and protection of montane areas and white-sand forests.

Estudios ornitológicos en Loreto, Perú: notas sobre su historia / Ornithological studies in Loreto, Peru: Notes on its history

Resumen En 1542 Orellana llega al Amazonas e inicia una historia de exploración continua de Loreto. Sin embargo, los estudios de las aves de este departamento recién empiezan en el siglo XIX con las exploraciones de Johann Baptist von Spix, Johann Jakob Von Tschudi, Francis-Louis de Castelnau, Emile Deville, Edward Bartlett, John Hauxwell y Henry Walter Bates quienes colectan para museos europeos y estadounidenses. En 1850 llega Antonio Raimondi al Perú incorporándose a la Universidad Nacional Mayor de San Marcos e iniciando los estudios nacionales de aves loretanas en 1859 y 1869. Raimondi colaboró activamente con Władysław Taczanowski, asistidos por Konstanty Jelski y Jan Stolzmann, cuyas colectas en Loreto fueran usadas en Ornithologie du Pérou, primer tratado de la avifauna peruana, conservándose algunos especímenes en el Museo de Historia Natural de la UNMSM (MHN). A inicios del siglo XX, Malcolm Anderson y Wilfred Osgood y posteriormente los hermanos Olalla colectaron para museos estadounidenses, marcando el inicio del predominio norteamericano en la ornitología en Perú. El investigador peruano Javier Ortiz de la Puente, primer jefe de la Sección Aves del MHN, realizó colectas en Loreto en 1948 y 1952, antes de su prematura muerte. María Koepcke, siguiente responsable de la Sección Aves del MHN, colecta en Pacaya-Samiria en 1967. John Patton O'Neill visita Loreto en 1963, iniciándose un interés que lo llevaría a impulsar la ornitología en Perú por varias décadas. La investigación continua de la avifauna loretana convirtió a esta región en un punto clave para entender los patrones de diversificación de las aves amazónicas.

Abstract In 1542 Orellana arrived in the Amazon and began a history of continuous exploration of Loreto. However, the birds' study of Loreto began in the 19th century with the explorations of Johann Baptist von Spix, Johann Jakob von Tschudi, Francis-Louis de Castelnau, Emile Deville, Edward Bartlett, John Hauxwell and Henry Walter Bates, who collected for European and American museums. In 1850 Antonio Raimondi arrived in Peru, joining the Universidad Nacional Mayor de San Marcos, and starting national studies of birds of Loreto in 1859 and 1869. Raimondi actively collaborated with Władysław Taczanowski, assisted by Konstanty Jelski and Jan Stolzmann, whose collections in Loreto were used in Ornithologie du Pérou, the first treatise on Peruvian avifauna. Some of these specimens are preserved in the Museo de Historia Natural of the UNMSM (MHN). At the beginning of the 20th century, Malcolm Anderson and Wilfred Osgood and later the Olalla brothers collected for American museums, marking the beginning of American predominance in ornithology in Peru. The Peruvian researcher Javier Ortiz de la Puente, the first head of the Bird Section of the MHN, collected in Loreto in 1948 and 1952, before his unfortunate death. María Koepcke, next head of the MHN Bird Section, collected in Pacaya-Samiria in 1967. John Patton O'Neill visited Loreto in 1963, initiating an interest that would lead him to promote ornithology in Peru for several decades. The continuous research of Loreto's avifauna turned this region into a key point to understand the diversification patterns of Amazonian birds.

Trypanosoma cruzi and Incidental Sarcocystis spp. in Endangered Ocelots (Leopardus pardalis) of South Texas, USA.

The federally endangered ocelot (Leopardus pardalis) population of south Texas, USA is declining; fewer than an estimated 80 ocelots remain. South Texas has robust transmission of Trypanosoma cruzi, the protozoan parasite causing Chagas disease in humans and various mammals. This parasite's impact in ocelots is unknown. Blood from live-trapped ocelots was collected by US Fish and Wildlife Service personnel in an annual monitoring program; additionally, tissues were obtained from carcasses collected from 2010 to 2017 around Laguna Atascosa National Wildlife Refuge in south Texas and placed in scientific collections. Variable samples were available from 21 ocelots: skeletal muscle (n=15), heart tissue (n=5), lung (n=1), kidney (n=1), spleen (n=1), liver (n=1), blood clot (n=9), and serum (n=3). Overall, 3/21 (14.3%) ocelots showed evidence of T. cruzi infection or exposure, with T. cruzi PCR-positive samples of skeletal muscle, heart, and blood clot, respectively. All three were infected with the T. cruzi discrete taxonomic unit "TcI"; one of these ocelots also had anti-T. cruzi antibodies. Lymphoplasmacytic inflammation was noted in the PCR-positive heart tissue and in some PCR-negative tissues from this and other individuals. Incidentally, Sarcocystis spp. were noted histologically in five ocelots. Trypanosoma cruzi infection and associated cardiac lesions suggest that this parasite should be further investigated in vulnerable populations.

Two new species of Smicridea (Rhyacophylax) Müller 1879 (Trichoptera: Hydropsychidae) from Atlantic Forest, Brazil.

Smicridea (Rhyacophylax) Müller 1879 contains 106 species, 41 of which occur in Brazil, mainly in the Atlantic Forest biome. Despite its diversity, it is estimated that many undescribed species are believed to be housed in scientific collections, waiting to be described. Smicridea specimens housed in two Brazilian insect collections (Coleção Entomológica Professor José Alfredo Pinheiro Dutra, Rio de Janeiro and Museu de Zoologia da Universidade de São Paulo, São Paulo) were examined and two new species of S. (Rhyacophylax) were detected. The objective of this study is to describe and illustrate these species. Smicridea (Rhyacophylax) boraceia sp. nov. and Smicridea (Rhyacophylax) dumasi sp. nov. inhabit the Atlantic forest biome and can be recognized mainly by the morphology of tergum X and the phallic apparatus. Six species groups are recognized in S. (Rhyacophylax); however, the two new species described here cannot be placed in any of these groups.

Área de conservación Guanacaste Echinoderms, North Pacific of Costa Rica / Equinodermos del Área de Conservación Guanacaste, Pacífico Norte de Costa Rica

Introduction: The study of the marine diversity of the North Pacific of Costa Rica began with isolated foreign expeditions in the 1930s and was systematically developed in the mid-1990s by the Center for Research in Marine Sciences and Limnology, Universidad de Costa Rica, as consequence there are now a total of 1 479 reported species in this region. Objective: Present an update to the echinoderm richness of the Guanacaste Conservation Area. Methods: We sampled 25 localities exhaustively and estimated similarity between sites based on the family richness and environmental heterogeneity. Results: We found 61 taxa, which represent 26 % of the echinoderm reported species for the country's Pacific coast. Of these, 43 species are new records for the Guanacaste Conservation Area, and seven for Costa Rica and Central American Pacific coasts. We found three morpho-species that do not match to available descriptions of the Eastern Tropical Pacific echinoderm species. We also found the holothuroid Epitomapta tabogae, and the ophiuroid Ophioplocus hancocki, previously thought endemic to Panama and the Galapagos Islands, respectively. The proximity of the sampled sites and the redundancy of certain families may explain why we did not find important differences among localities. Conclusions: The echinoderm richness of this conservation area is at least 20 % higher than previously reported, reaching similar levels to those in other high diversity sites of the Eastern Tropical Pacific.

Introducción: El estudio de la diversidad marina del Pacífico Norte de Costa Rica inició con expediciones extranjeras aisladas en la década de 1930, y fue desarrollado sistemáticamente a mediados de la década de 1990 por el Centro de Investigaciones en Ciencias del Mar y Limnología de la Universidad de Costa Rica, como consecuencia ahora se reporta un total de 1 479 especies en esta región. Objetivo: Presentar una actualización de la riqueza de equinodermos del Área de Conservación Guanacaste. Métodos: Realizamos muestreos exhaustivos en 25 localidades y estimamos la similitud entre sitios con base en la riqueaza de familias y la heterogeneidad ambiental. Resultados: Encontramos 61 taxa, que representan el 26% de las especies reportadas para la costa pacífica del país. De estas, 43 especies son nuevos registros para el Área de Conservación Guanacaste y siete para las costas de Costa Rica y el Pacífico centroamericano. Tres morfoespecies no coinciden con las descripciones disponibles para las especies del Pacífico Tropical Oriental. Por último, hallamos un ejemplar del holoturoideo Epitomapta tabogae y otro del ofiuroideo Ophioplocus hancocki, considerados endémicos para Panamá y las Islas Galápagos respectivamente. La proximidad entre los sitios muestreados y la redundancia de ciertas familias pueden explicar por qué no se encontraron diferencias entre las localidades. Conclusiones: La riqueza de equinodermos de esta área de conservación es al menos 20% mayor que la reportada anteriormente, alcanzando niveles similares a los de otros sitios de alta diversidad del Pacífico Tropical Oriental.

Body mass data set for 1,317 bird and 270 mammal species from Colombia.

Body mass is one of the most important phenotypic attributes in animal ecology and life history. This trait is widely used in the fields of ecology and macroevolution, since it influences physiology, morphological functions, and a myriad of ecological and social interactions. In this data set, our aim was to gather a comprehensive bird and mammal body mass data set from northern South America. We report body mass, discriminated by sex, for 42,022 individual birds and 7,441 mammals representing 1,317 bird species (69% of Colombia's avifauna) and 270 mammal species (51% of Colombian mammals) from the Neotropics. The data were sourced from vouchers collected between 1942 and 2020 and from individuals captured and released at banding stations over the last two decades for birds (2000-2020) and the last decade for mammals (2010-2020), by 10 research groups and institutions in Colombia. This data set fills gaps identified in other similar databases, as it focuses on northern South America, a highly diverse Neotropical region often underrepresented in morphological data sets. We provide wide taxonomic coverage for studies interested in information both at regional and local scales. There are no copyright restrictions; the present data paper should be appropriately cited when data are used for publication. The authors would appreciate learning about research projects, teaching exercises, and other uses given to this data set and are open to contribute in further collaborations using these data.

Catalogue, distribution, taxonomic notes, and conservation of the Western Palearctic endemic hunchback beetles (Tenebrionidae, Misolampus).

Hunchback darkling beetles of the Ibero-Maghrebian genus Misolampus Latreille, 1807 (Tenebrionidae, Stenochiinae) encompass six species: M. gibbulus (Herbst, 1799), M. goudotii Guérin-Méneville, 1834, M. lusitanicus Brême, 1842, M. ramburii Brême, 1842, M. scabricollis Graells, 1849, and M. subglaber Rosenhauer, 1856. Previously known distribution ranges of the species were delineated using many old records, the persistence of such populations being questionable under the current situation of global biodiversity loss. Additionally, the status of geographically isolated populations of the genus have been the subject of taxonomic controversy. An exhaustive bibliographical revision and field search was undertaken, and the Misolampus collection of the Museo Nacional de Ciencias Naturales (MNCN-CSIC) was revised. The aims are to (i) provide an updated geographic distribution range for the species of Misolampus; (ii) to determine the taxonomic status of controversial populations; (iii) to provide a catalogue for Misolampus; and (iv) to discuss the conservation status of these saproxylic beetles. As a result, a catalogue including synonymies and type localities, geographical records, diagnoses, and information on natural history for all species of Misolampus is presented. The results reveal that the distribution ranges of the species of Misolampus have not undergone a reduction in the last century, and indicate the presence of the genus in areas where it had never been recorded before. The morphological variability of M. goudotii drove the proposal of different taxa that are here formally synonymised as follows: M. goudotii Guérin-Méneville, 1834 = M. erichsoni Vauloger de Beaupré, 1900, syn. nov. = M. peyerimhoffi Antoine, 1926, syn. nov.

Documentation of the Expansion of the Gulf Coast Tick (Amblyomma maculatum) and Rickettsia parkeri: First Report in Illinois.

The Gulf Coast tick, Amblyomma maculatum, is of public and veterinary health concern, as it is the primary vector of Rickettsia parkeri and Hepatozoon americanum, causative agents of Rickettsiosis and American canine hepatozoonosis. The Gulf Coast tick's range has expanded over the last 50 yr into the mid-Atlantic states, and its expansion is expected to continue northward. We are reporting the presence of A. maculatum for the first time in Illinois, including a total of 18 specimens collected at 6 different sites during surveys in 2013 and 2019. Fourteen of these specimens were screened for Rickettsia parkeri, which resulted in the detection of this bacteria in 8 samples from 4 counties. By depositing these specimens in scientific collections, we provide materialistic evidence of their establishment in 2 counties. We urge health officials to rely on and use scientific collections to document the expansion of these and other vectors across the country. Additionally, we recommend that health practitioners become aware of the clinical similarities between Rocky Mountain Spotted Fever (caused by Rickettsia rickettsii) and "tidewater" fever (caused by R. parkeri).

Rediscovering a forgotten scientific collection in the Rio Grande do Norte State, Brazil: The herpetological collection of the zoologist José Santiago Lima-Verde / Redescobrindo uma coleção científica esquecida no Estado do Rio Grande do Norte, Brasil: A coleção herpetológica do zoólogo José Santiago Lima-Verde

Abstract: The zoologist Dr. José Santiago Lima-Verde (1945-2019) made a number of major contributions to the field of animal biology in the northeastern Brazil, in particular through his research with snakes. While employed as a professor at Escola Superior de Agricultura de Mossoró, currently the Universidade Federal Rural do Semi-Árido, Prof. Lima-Verde established a herpetological collection which remained forgotten for a number of decades. In the present study, we describe the revitalization of this collection, which included the updating of the identification of the species and the compilation of the metadata on the collection, which is now named the Coleção Herpetológica Lima-Verde. We catalogued 80 specimens representing 30 species, including 23 species of snakes, five lizards, and two amphisbaenians. One third (30%) of the specimens had information on dates and sites, with the majority of these specimens being collected in the Brazilian State of Rio Grande do Norte during the 1970's. We also catalogued 315 eggs of seven snake species, from 22 clutches laid in captivity. The collection presents unpublished data and will constitute a reference for future herpetological research on the species found in western region of State of Rio Grande do Norte. Our findings reinforce the importance of zoological collections for the understanding of patterns of biodiversity, and we would recommend the creation of more regional scientific collections and the broader recognition of their value as a basic source of biological data.

Resumo: O zoólogo Dr. José Santiago Lima-Verde (1945-2019) fez uma série de importantes contribuições para o campo da biologia animal no nordeste do Brasil, em particular através de sua pesquisa com serpentes. Enquanto professor da Escola Superior de Agricultura de Mossoró, atualmente Universidade Federal Rural do Semi-Árido, o Prof. Lima-Verde estabeleceu uma coleção herpetológica que permaneceu esquecida por várias décadas. No presente estudo, descrevemos a revitalização dessa coleção, que incluiu a atualização da identificação das espécies e a compilação dos metadados da coleção, que agora é denominada Coleção Herpetológica Lima-Verde. Nós catalogamos 80 espécimes representando 30 espécies, incluindo 23 espécies de serpentes, cinco lagartos e duas anfisbenas. Um terço (30%) dos exemplares possuíam informações sobre datas e locais de coleta, sendo a maioria desses espécimes coletados no Estado do Rio Grande do Norte durante a década de 1970. Também catalogamos 315 ovos de sete espécies de serpentes, provenientes de 22 ninhadas obtidas em cativeiro. A coleção apresenta dados inéditos e constituirá uma referência para futuras pesquisas herpetológicas sobre as espécies encontradas na região oeste do Estado do Rio Grande do Norte. Nossos achados reforçam a importância das coleções zoológicas para a compreensão dos padrões de biodiversidade, e nós recomendamos a criação de mais coleções científicas regionais e o reconhecimento mais amplo de seu valor como fonte básica de dados biológicos.

Geographical distribution of Lasaia Bates, 1868 (Lepidoptera: Riodinidae) across the biogeographical provinces of Mexico.

Riodinidae are one of the most diverse families of Lepidoptera, mainly in the Neotropical region; however, their biology, ethology, taxonomy, systematics, and biogeography are poorly known. In Mexico, the regional and local distributions of the family are still incomplete. We review the distributional data of the genus Lasaia Bates (Lepidoptera: Riodinidae), based on records from four national and seven international collections. We record five species and seven subspecies in Mexico, with 2722 records, distributed in 314 localities of 24 states. The states with higher species richness are Chiapas, Oaxaca, and Veracruz; also, the genus was recorded in 11 of the 14 biogeographical provinces of Mexico. The tropical semi-deciduous and deciduous forests, below of 1000 m a.s.l., contain most of the diversity of Lasaia. Historical data are crucial for the study of local and regional diversity and ecological patterns at large temporal scales. Data presented here show the morphological and ecological variation of Lasaia over the last 80 years, mostly from the XX century when anthropogenic disturbances were intensified. This kind of studies is the first step in recording the historical distribution of these taxa, which will lead to more complex analyses on distribution range shifts, their causes and consequences.

Tapping into technology and the biodiversity informatics revolution: updated terrestrial mammal list of Angola, with new records from the Okavango Basin.

Using various sources, including the Global Biodiversity Information Facility (GBIF), published literature, recent (2015-2017) collections, as well as bat detector and camera trap surveys with opportunistic sightings and live capture in the upper Okavango catchment in central Angola, we present an updated mammal checklist of 275 species from 15 different orders for Angola (including the Cabinda region). Recent surveys (captures and bat detectors) of small mammals from the upper Okavango catchment yielded 46 species (33 species of bats, ten species of rodents and three species of shrews). One bat (Pipistrellusrusticus, rusty pipistrelle); two rodents (Mussetzeri, Setzer's mouse and Zelotomyswoosnami, Woosnam's broad-faced mouse) and one shrew (Suncusvarilla, lesser dwarf shrew) were captured for the first time, in Angola. While our species lists of bats conformed to predicted totals, terrestrial small mammals were under sampled, with only 13 species recorded by our trapping survey compared to a total of 42 shrew and rodent species expected based on GBIF records for the central Angolan highlands. Seven terrestrial small mammal species (one shrew and six rodents) are endemic to the central and western Angolan highlands but none of these were captured in our survey. The bat detector surveys added three further bat species to the country list: Pipistrellushesperidus, Kerivoulaargentata, and Mopsmidas. Camera trap surveys and opportunistic sightings in the upper Okavango catchment in 2016 yielded a total of 35 species of medium-large mammals, from 17 families, although all of these had been reported previously in Angola. GBIF proved to be an excellent source of biodiversity data for Angolan mammals, most importantly for documenting dramatic historical range changes of larger mammals such as the sable (Hippotragusnigerniger), Kirk's sable (H.nigerkirkii) and the giant sable (H.nigervariani).

Catalogue of Lygistorrhinidae (Diptera: Bibionomorpha) types housed in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil.

This paper provides a catalogue of the type specimens of Lygistorrhinidae (Diptera: Bibionomorpha) held in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil (MZUSP). Label information and type condition of the six type specimens (three holotypes and three paratypes) of four species are provided, along with high resolution images of the types and their labels.

An eighteenth century travelling theodolite / Um teodolito viajante do século XVIII

Abstract An old topographic compass displayed in a showroom of the Museu de Astronomia e Ciências Afins (MAST), in Rio de Janeiro, Brazil, took our curiosity namely because of its resemblance to a theodolite, described by J.H. de Magellan. Not many things were known about its previous history. From the different documents studied, and the characteristics of this singular theodolite, it must have belonged to the collections of instruments acquired for the Brazilian border demarcations undertaken after the Santo Ildefonso Treaty, agreed to by the Portuguese and Spanish courts in 1777. Several instruments were bought in London, and supervised and chosen by Magellan, the Portuguese instruments expert. We present arguments in favour of this conclusion.

Resumo Um antigo compasso topográfico em exibição no Museu de Astronomia e Ciências Afins (Mast), no Rio de Janeiro, chamou-nos a atenção por sua semelhança com um teodolito descrito por João Jacinto de Magalhães. Pouco era conhecido sobre sua história prévia. De acordo com os diversos documentos estudados e as caracteríticas desse único teodolito, ele deve ter pertencido às coleções de instrumentos adquiridos para a demarcação das fronteiras brasileiras realizadas após o Tratato de Santo Ildefonso, acordado entre as cortes portuguesa e espanhola em 1777. Vários instrumentos foram comprados em Londres, supervisionados e escolhidos por Magalhães, o perito português em instrumentos. Apresentamos argumentos que corroboram essa conclusão.

Catalogue of Anisopodidae (Diptera, Bibionomorpha) types housed in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil

ABSTRACT Following a recommendation of the International Code of Zoological Nomenclature, this paper provides a catalogue of the type specimens of Anisopodidae (Diptera: Bibionomorpha) held in the collection of the Museu de Zoologia da Universidade de São Paulo, Brazil (MZUSP). Information on labels and type conditions, on 54 type specimens (including 21 primary types) of 24 Neotropical species are provided.