Andean Crops Germination: Changes in the Nutritional Profile, Physical and Sensory Characteristics. A Review.

Andean crops such as quinoa, amaranth, cañihua, beans, maize, and tarwi have gained interest in recent years for being gluten-free and their high nutritional values; they have high protein content with a well-balanced essential amino acids profile, minerals, vitamins, dietary fiber, and antioxidant compounds. During the germination bioprocess, the seed metabolism is reactivated resulting in the catabolism and degradation of macronutrients and some anti-nutritional compounds. Therefore, germination is frequently used to improve nutritional quality, protein digestibility, and availability of certain minerals and vitamins; furthermore, in specific cases, biosynthesis of new bioactive compounds could occur through the activation of secondary metabolic pathways. These changes could alter the technological and sensory properties, such as the hardness, consistency and viscosity of the formulations prepared with them. In addition, the flavor profile may undergo improvement or alteration, a critical factor to consider when integrating sprouted grains into food formulations. This review summarizes recent research on the nutritional, technological, functional, and sensory changes occur during the germination of Andean grains and analyze their potential applications in various food products.

Biostimulated-sesame sprout extracts as potential agents against Leishmania mexicana.

Leishmania mexicana is one of the causal agents of cutaneous leishmaniasis. Current antileishmanial chemotherapeutics have demonstrated adverse side effects; thus, alternative treatments are needed. In this study, we performed in silico and in vitro analyses of the leishmanicidal potential of the most abundant phenolic compounds identified in black sesame sprouts biostimulated with Bacillus clausii. The molecular docking analysis showed strong interactions (binding free energies between -6.5 and -9.5 kcal/mol) of sesaminol 2-O-triglucoside, pinoresinol dihexoside, isoverbascoside, and apigenin with the arginase, leishmanolysin, cysteine peptidase B, and pyruvate kinase leishmanial enzymes. Furthermore, almost all phenolic compounds interacted with the active site residues of L. mexicana enzymes. In vitro, the B. clausii-biostimulated sprout phenolic extracts and apigenin inhibited the growth of promastigotes with IC50 values of 0.08 mg gallic acid equivalent/mL and 6.42 µM (0.0017 mg/mL), respectively. Additionally, in the macrophage infection model, cells treated with B. clausii-biostimulated sprout phenolic extracts and infected with L. mexicana exhibited significantly (P < 0.05) reduced nitric oxide production and decreased parasite burden. Altogether, our study provides important data related to high efficacy and less toxic natural antileishmanial candidates against promastigotes of L. mexicana.

Changes in phenolics and flavonoids in amaranth and soybean sprouts after UV-C treatment.

Sprouts, mainly from cereals, legumes, and some pseudo-cereals, are rich in nutrients and contain biocompounds, making them attractive for consumption. This research study aimed to develop treatments with UV-C light in soybean and amaranth sprouts and evaluate their effect on biocompounds content, compared with chlorine treatments. UV-C treatments were applied at distances of 3 and 5 cm and times of 2.5, 5, 10, 15, 20, and 30 min, whereas chlorine treatments were applied as immersion in solutions at 100 and 200 ppm for 15 min. Phenolics and flavonoid content were higher in UV-C-treated sprouts than in those treated with chlorine solutions. Ten biocompounds were identified in soybean sprouts, with increasing in apigenin C-glucoside-rhamnoside (105%), apigenin 7-O-glucosylglucoside (237%), and apigenin C-glucoside malonylated (70%) due to UV-C application (3 cm, 15 min); for amaranth sprouts, five biocompounds were identified, with higher contents of p-coumaroylquinic acid (17.7%) after UV-C treatment (3 cm, 15 min). The best treatment to achieve the highest bioactive compounds concentration was UV-C at a distance of 3 cm for 15 min, without significant modification on the color parameters, Hue and chroma. PRACTICAL APPLICATION: UV-C can be used to increase the biocompound content in amaranth and soybean sprouts. Nowadays, there is UV-C equipment available for industrial applications. In this way, sprouts may be maintained as fresh through this physical technology, and they will retain or increase the concentration of health-related compounds.

Characterization of yellow pitahaya (Selenicereus megalanthus Haw) genotypes under two productive systems in Colombia / Caracterização de genótipos de pitaia amarela (Selenicereus megalanthus Haw) a partir de dois sistemas produtivos na Colômbia

Selenicereus megalanthus Haw. It is an exotic fruit tree, with productive and nutritional potential. In Colombia, there is a great phenotypic and genotypic diversity, but its genetic studies are scarce. The objective was to characterize morphologically 15 selected yellow pitahaya genotypes, under two productive systems in the open field and under cover, in the municipalities of Miraflores and Zetaquira, in Boyacá, Colombia. Quantitative characters were evaluated: plant height (PH), number of vegetative sprouts (NVS), sub-sprouts (SS), longest sprouts length (LSL), distance between areoles (DBA), width of the ribs in the apical region (WRA), width of the ribs in the middle region (WRM), width of the ribs in the basal region (WRB), height of undulations between successive areoles in a rib (HUA), number of spines per areole (NSA) and longest spine length (LSP). The results showed under the two productive systems and the evaluated localities that the variables with the highest coefficient of variation (greater than 90%) were the number of sub-sprouts, height of the undulations between successive areoles (HUA) and the longest spine length (LSP). High positive correlations were obtained between the distances areoles, the width of the ribs and the length of the spines (r>0.7). The conglomerate showed that the characteristics that define the groupings are height of the plant, the texture of the cladodes, the width of the ribs and the height of the undulations. Characters associated with the shoots and cladodes were identified, which directly influence the vegetative propagation and therefore the yield of the yellow pitahaya.

Selenicereus megalanthus Haw é uma fruteira exótica, com potencial produtivo e nutricional. Na Colômbia existe uma grande diversidade fenotípica e genotípica, mas seus estudos genéticos são escassos. O objetivo deste trabalho foi caracterizar morfologicamente 15 genótipos selecionados de pitaya amarela, a partir de dois sistemas produtivos em campo aberto e sob cobertura, nos municípios de Miraflores e Zetaquira, em Boyacá, Colômbia. Foram avaliados os caracteres quantitativos: altura da planta (PH), número de brotos vegetativos (NVS), sub-brotos (SS), maior comprimento dos brotos (LSL), distância entre aréolas (DBA), largura das nervuras na região apical (WRA), largura das costelas na região média (WRM), largura das costelas na região basal (WRB), altura das ondulações entre aréolas sucessivas em uma costela (HUA), número de espinhos por aréola (NSA) e maior espinha comprimento (LSL). Os resultados mostraram nos dois sistemas produtivos e nas localidades avaliadas que as variáveis com maior coeficiente de variação (maior que 90%) foram o número de sub-brotos, altura das ondulações entre aréolas sucessivas (HUA) e o maior comprimento do espinho (LSL). Altas correlações positivas foram obtidas entre as distâncias entre as aréolas, a largura das costelas e o comprimento dos espinhos (r > 0.07). O conglomerado mostrou que as características que definem os agrupamentos são a altura da planta, a textura dos cladódios, a largura das nervuras e a altura das ondulações. Foram identificados caracteres associados à brotação e aos cladódios, que influenciam diretamente na propagação vegetativa e, consequentemente, na produção da pitaya-amarela.

Maximization of Sulforaphane Content in Broccoli Sprouts by Blanching.

Broccoli sprouts are a recognized source of health-promoting compounds, such as glucosinolates, glucoraphanin, and sulforaphane (SFN). Maximization of SFN content can be achieved by technological processing. We investigated the effect of blanching conditions to determine the optimal treatment that maximizes sulforaphane content in broccoli sprouts. Broccoli seeds (cv. Traditional) grown under controlled conditions were harvested after 11 days from germination and subjected to different blanching conditions based on a central composite design with temperature and time as experimental factors. Results were analyzed by ANOVA followed by a Tukey test. The optimum conditions were identified through response surface methodology. Blanching increased sulforaphane content compared with untreated sprouts, agreeing with a decrease in total glucosinolates and glucoraphanin content. Temperature significantly affected SFN content. Higher temperatures and shorter immersion times favor glucoraphanin hydrolysis, thus increasing SFN content. The optimum conditions were blanching at 61 °C for 4.8 min, resulting in 54.3 ± 0.20 µmol SFN/g dry weight, representing a 3.3-fold increase with respect to untreated sprouts. This is the highest SFN content reported for sprouts subjected to any treatment so far. The process described in this work may contribute to developing functional foods and nutraceuticals that provide sulforaphane as an active principle.

Selenium, Sulfur, and Methyl Jasmonate Treatments Improve the Accumulation of Lutein and Glucosinolates in Kale Sprouts.

Kale sprouts contain health-promoting compounds that could be increased by applying plant nutrients or exogenous phytohormones during pre-harvest. The effects of selenium (Se), sulfur (S), and methyl jasmonate (MeJA) on lutein, glucosinolate, and phenolic accumulation were assessed in kale sprouts. Red Russian and Dwarf Green kale were chamber-grown using different treatment concentrations of Se (10, 20, 40 mg/L), S (30, 60, 120 mg/L), and MeJA (25, 50, 100 µM). Sprouts were harvested every 24 h for 7 days to identify and quantify phytochemicals. The highest lutein accumulation occurred 7 days after S 120 mg/L (178%) and Se 40 mg/L (199%) treatments in Red Russian and Dwarf Green kale sprouts, respectively. MeJA treatment decreased the level of most phenolic levels, except for kaempferol and quercetin, where increases were higher than 70% for both varieties when treated with MeJA 25 µM. The most effective treatment for glucosinolate accumulation was S 120 mg/L in the Red Russian kale variety at 7 days of germination, increasing glucoraphanin (262.4%), glucoerucin (510.8%), 4-methoxy-glucobrassicin (430.7%), and glucoiberin (1150%). Results show that kales treated with Se, S, and MeJA could be used as a functional food for fresh consumption or as raw materials for different industrial applications.

Chia seeds and chemical-elicited sprouts supplementation ameliorates insulin resistance, dyslipidemia, and hepatic steatosis in obese rats.

Chia seeds (CS) and sprouts are rich in bioactive compounds. This study aimed to assess the effects of germination and chemical elicitation (salicylic acid [SA]; hydrogen peroxide [H2 O2 ]) on proximate chemical, total phenolics compounds (TPC), non-extractable proanthocyanidins (NEPA), and carotenoids content of chia sprouts; besides, the effects of their supplementation on obesity-associated complications in rats fed with high-fat and fructose diet (HFFD) were evaluated. Protein, carbohydrate, TPC, NEPA, and carotenoids content were higher in sprouts than CS; elicitation enhanced TPC and carotenoids compared to non-elicited (NE) sprouts. CS, NE, and elicited chia sprouts ameliorated insulin resistance and dyslipidemia at the same level in HFFD-fed rats. NE and SA-chia sprouts exerted the biggest reduction in hepatic triglycerides, which could be partially related to inhibition of pancreatic lipase activity. In addition, SA elicitation induced the greatest effect on insulin levels and corporal weight. CS and their sprouts decreased obesity and its complication, mainly SA-elicited sprouts. PRACTICAL APPLICATIONS: The growing epidemic of non-communicable diseases such as diabetes and obesity has led to the search for prevention and treatment through lifestyle changes, including the consumption of foods rich in bioactive compounds, such as seeds and their sprouts. Since sprouts contain higher concentrations of bioactive compounds and nutrients than seed, germination is a natural alternative to produce ready-to-eat functional foods. Chemical elicitation is a strategy to increase even more the bioactivity of sprouts. CS has been recognized for its beneficial health effects ameliorating dyslipidemia and insulin resistance. This study demonstrates that elicitation, with SA and H2 O2 , during germination of CS, increases the nutrient and phytochemical content of sprouts, with beneficial effects on body weight gain, insulin resistance, dyslipidemia, and prevention of NAFLD progression in diet-induced obese rats. Therefore, chia sprouts, natural and elicited, may be used as potential nutraceutical foods for the prevention and treatment of obesity and its complications.

Amaranth Seeds and Sprouts as Functional Ingredients for the Development of Dietary Fiber, Betalains, and Polyphenol-Enriched Minced Tilapia Meat Gels.

There is an increasing interest in the development of meat processed products enriched with antioxidant dietary fiber to augment the consumption of these health beneficial compounds. This study aimed to evaluate the nutritional, nutraceutical, and antioxidant potential, as well as the physicochemical properties of minced tilapia fillets (meat) gels with added amaranth seed or sprout flours (0%, 2%, 4%, 8%, and 10% w/w). Dietary fiber content was significantly increased with the addition of amaranth seed (1.25-1.75-fold) and sprout flours (1.99-3.21-fold). Tilapia gels with added 10% amaranth seed flour showed a high content of extractable dihydroxybenzoic acid and cinnamic acid, whereas the addition of 10% amaranth sprout flour provided a high and wide variety of bioactive compounds, mainly amaranthine and bound ferulic acid. The addition of amaranth seed and sprout flours increased hardness (1.01-1.73-fold) without affecting springiness, decreased luminosity (1.05-1.15-fold), and increased redness and yellowness. Therefore, amaranth seed and sprout flours could be used as functional ingredients for the development of fish products rich in bioactive compounds.

Comparative analysis of trophoblasts and angiogenesis in human placental compartments / Análisis comparativo de trofoblastos y angiogénesis en compartimentos placentarios humanos

SUMMARY: Trophoblasts perform different functions depending on their location. This study aimed to obtain structural clues about the functions of villous and extravillous trophoblasts by using light and electron microscopy. Term placenta samples were obtained from 10 healthy pregnant women following cesarean sections. Frozen sections were stained with hematoxylin-eosin, semi- thin sections were stained with toluidine blue and examined with a light microscope, while thin sections were contrasted using uranyl acetate-lead citrate and evaluated under an electron microscope. Fine structural features of villous trophoblasts overlapped some villous stromal cells. In addition to the usual appearance of mature capillaries in villous stroma, we demonstrated and reported maturational stages of angiogenetic sprouts in term placenta. Extravillous trophoblasts were classified according to their location: fibrinoid, chorion, trophoblastic, column, maternal vascular endothelium, or decidua. All of these trophoblasts shared some ultrastructural features but also were distinct from each other. In decidua, it was noted that the endothelial lining of some vessels was invaded by a few endovascular trophoblasts with irregular microvilli. These cells shared some ultrastructural properties with both villous trophoblasts and stromal cells. Examination showed that angiogenesis was still present in term placentas and that trophoblasts, endothelial and stromal cells have very similar properties ultrastructurally, suggesting they represent transformational forms.

RESUMEN: Los trofoblastos dependiendo de su ubicación realizan diferentes funciones. Este estudio tuvo como objetivo obtener pistas estructurales sobre las funciones de los trofoblastos vellosos y extravellosos mediante el uso de microscopía óptica y electrónica. Se obtuvieron muestras de placenta a término de 10 mujeres embarazadas sanas después de cesáreas. Las secciones congeladas se tiñeron con hematoxilina-eosina, las secciones semidelgadas se tiñeron con azul de toluidina y se examinaron con un microscopio óptico, mientras que las secciones delgadas se contrastaron con acetato de uranilo-citrato de plomo y se evaluaron con un microscopio electrónico. Las finas características estructurales de los trofoblastos vellosos se superponen a algunas células estromales vellosas. Además de la apariencia habitual de capilares maduros en el estroma velloso, demostramos e informamos etapas de maduración de brotes angiogenéticos en la placenta a término. Los trofoblastos extravellosos se clasificaron según su localización: fibrinoide, corion, trofoblástico, columna, endotelio vascular materno o decidua. Todos estos trofoblastos compartían algunas características ultraestructurales, pero también eran distintos entre sí. En decidua se observó que el revestimiento endotelial de algunos vasos estaba invadido por unos pocos trofoblastos endovasculares con microvellosidades irregulares. Estas células compartían algunas propiedades ultraestructurales tanto con los trofoblastos vellosos como con las células del estroma. El examen mostró que la angiogénesis todavía estaba presente en las placentas a término y que los trofoblastos, las células endoteliales y estromales tienen propiedades ultraestructurales muy similares, lo que sugiere que representan formas de transformación.

Chia Sprouts Elicitation with Salicylic Acid and Hydrogen Peroxide to Improve their Phenolic Content, Antioxidant Capacities In Vitro and the Antioxidant Status in Obese Rats.

Elicitation is a biotechnological approach to improve phenolic compounds content and antioxidant properties of ready-to-eat functional foods. This study aimed to evaluate the chemical elicitation effects using salicylic acid (SA) and hydrogen peroxide (H2O2) in optimized-germination conditions on seedling vigor, phenolic content, and their antioxidant capacities in vitro and serum and urine of Wistar obese rats. Optimized-germination conditions of 26.5 °C and 178 h produced a 64% of germination and a sprout length of 56 mm. Only, the elicitation with H2O2 (20 mM) enhanced the germination (75%) and H2O2 (10 and 20 mM) the sprout length (69 and 59 mm, respectively). In contrast, both elicitors enhanced phenolic contents, being more significant total phenolic compounds content for SA (1 and 2 mM), up to 65.5-73.5%. SA and H2O2 improved total flavonoids content (36.5-64.1%), ABTS (19.3-61.1%), and DPPH capacities (51-86%), depending on SA and H2O2 concentration, compared with non-elicited chia sprouts. The QUENCHER antioxidant capacities of elicited chia sprouts increased up to three times more than extracts capacities, principally Q-ABTS, which could be attributed to phenolic bounds to dietary fiber. Rats fed with a high-fat and fructose diet (HFFD) and supplemented with chia sprouts, especially 1-mM SA, improve the obesity-related oxidative stress through an increase of antioxidant capacities, using DPPH and ABTS test, on serum (70-118%) and urine samples (80-116%). These results suggest that chia sprouts elicited with 1-mM SA are a source of antioxidant compounds that can be used to decrease obesity related oxidative stress.

Improving Polyphenolic Compounds: Antioxidant Activity in Chickpea Sprouts through Elicitation with Hydrogen Peroxide.

Elicitation appears to be a promising alternative to enhance the bioactive compound content and biological activities of legume sprouts. Multi-response optimization by response surface methodology (RSM) with desirability function (DF) was used to optimize the elicitor concentration (hydrogen peroxide (H2O2)) and germination time in order to maximize total phenolic content (TPC), total flavonoids content (TFC), and antioxidant activity (AOX) of chickpea sprouts. Chemical, antinutritional, and nutraceutical properties of optimized chickpea sprouts (OCS) were also determined. The predicted regression models developed were efficiently fitted to the experimental data. The results of the desirability function revealed that optimum attributes in chickpea sprouts can be achieved by the application of 30 mM H2O2 and 72 h of germination time, with global desirability value D = 0.893. These OCS had higher (p < 0.05) TPC (7.4%), total iso-flavonoids (16.5%), AOX (14.8%), and lower phytic acid (16.1%) and saponins (21.8%) compared to H2O2 non-treated chickpea sprouts. Optimized germination conditions slightly modified the flavonoid profile in chickpea; eight iso-flavonoids were identified in OCS, including formononetin and biochanin A, which were identified as the major compounds. Results from this study support elicitation with H2O2 as an effective approach to improve phytochemical content and antioxidant activity in chickpea sprouts.

UVA, UVB Light Doses and Harvesting Time Differentially Tailor Glucosinolate and Phenolic Profiles in Broccoli Sprouts.

Broccoli sprouts contain health-promoting glucosinolate and phenolic compounds that can be enhanced by applying ultraviolet light (UV). Here, the effect of UVA or UVB radiation on glucosinolate and phenolic profiles was assessed in broccoli sprouts. Sprouts were exposed for 120 min to low intensity and high intensity UVA (UVAL, UVAH) or UVB (UVBL, UVBH) with UV intensity values of 3.16, 4.05, 2.28 and 3.34 W/m², respectively. Harvest occurred 2 or 24 h post-treatment; and methanol/water or ethanol/water (70%, v/v) extracts were prepared. Seven glucosinolates and 22 phenolics were identified. Ethanol extracts showed higher levels of certain glucosinolates such as glucoraphanin, whereas methanol extracts showed slight higher levels of phenolics. The highest glucosinolate accumulation occurred 24 h after UVBH treatment, increasing 4-methoxy-glucobrassicin, glucobrassicin and glucoraphanin by ~170, 78 and 73%, respectively. Furthermore, UVAL radiation and harvest 2 h afterwards accumulated gallic acid hexoside I (~14%), 4-O-caffeoylquinic acid (~42%), gallic acid derivative (~48%) and 1-sinapoyl-2,2-diferulolyl-gentiobiose (~61%). Increases in sinapoyl malate (~12%), gallotannic acid (~48%) and 5-sinapoyl-quinic acid (~121%) were observed with UVBH Results indicate that UV-irradiated broccoli sprouts could be exploited as a functional food for fresh consumption or as a source of bioactive phytochemicals with potential industrial applications.

Survival of Salmonella Typhimurium on soybean sprouts following treatments with gaseous chlorine dioxide and biocontrol Pseudomonas bacteria.

Control of Salmonella Typhimurium on sprouts is crucial for food and consumer safety. In this study, natural microflora on soybean seed was assessed and effects of gaseous chlorine dioxide (ClO2) and biocontrol Pseudomonas on the survival of S. Typhimurium on soybean sprouts were evaluated. Sprouts were dip-inoculated with S. Typhimurium prior to the application of the biocontrol (P. chlororaphis and P. fluorescens). After inoculation with S. Typhimurium, the sprouts were treated with ClO2 at 0.4 mg/L for 1 h (90% R.H., 13°C). Pseudomonas strains and Salmonella were recovered on Pseudomonas Agar F (PAF) and xylose lysine tergitol-4 (XLT-4) media, respectively. Pseudomonas strains reduced Salmonella by <1 log colony forming units (CFU)/g of sprouts, whereas S. Typhimurium on soybean sprouts was reduced from 2.55 to 5.35 logs CFU/g by ClO2. Gaseous ClO2 treatment reduced S. Typhimurium by 3.90 (0 h), 4.47 (24 h), and 3.61 log CFU/g (168 h). It was concluded that ClO2 and biocontrol treatment can enhance sprout safety.

Effect of chemical stress on germination of cv Dalia bean (Phaseolus vularis L.) as an alternative to increase antioxidant and nutraceutical compounds in sprouts.

The aim of this study was to determine the effect of chitosan (CH), salicylic acid (SA) and hydrogen peroxide (H2O2) at different concentrations on the antinutritional and nutraceutical content, as well as the antioxidant capacity of bean sprouts (cv Dalia). All elicitors at medium and high concentrations reduced the antinutritional content of lectins (48%), trypsin inhibitor (57%), amylase inhibitor (49%) and phytic acid (56%). Sprouts treated with CH, SA and H2O2 (7µM; 1 and 2mM, and 30mM respectively) increased the content of phenolic compounds (1.8-fold), total flavonoids (3-fold), saponins (1.8-fold) and antioxidant capacity (37%). Furthermore, the UPLC-ESI-MS/MS analysis showed an increase of several nutraceutical compounds in bean sprouts treated with SA such as coumaric (8.5-fold), salicylic (115-fold), gallic (25-fold) and caffeic (1.7-fold) acids, as well as epigallocatechin (63-fold), rutin (41-fold) and quercetin (16.6-fold) flavonoids. The application of elicitors in bean seed during sprouting enhances their nutraceutical properties.

Low temperature conditioning of garlic (Allium sativum L.) "seed" cloves induces alterations in sprouts proteome.

Low-temperature conditioning of garlic "seed" cloves substitutes the initial climatic requirements of the crop and accelerates the cycle. We have reported that "seed" bulbs from "Coreano" variety conditioned at 5°C for 5 weeks reduces growth and plant weight as well as the crop yields and increases the synthesis of phenolic compounds and anthocyanins. Therefore, this treatment suggests a cold stress. Plant acclimation to stress is associated with deep changes in proteome composition. Since proteins are directly involved in plant stress response, proteomics studies can significantly contribute to unravel the possible relationships between protein abundance and plant stress acclimation. The aim of this work was to study the changes in the protein profiles of garlic "seed" cloves subjected to conditioning at low-temperature using proteomics approach. Two sets of garlic bulbs were used, one set was stored at room temperature (23°C), and the other was conditioned at low temperature (5°C) for 5 weeks. Total soluble proteins were extracted from sprouts of cloves and separated by two-dimensional gel electrophoresis. Protein spots showing statistically significant changes in abundance were analyzed by LC-ESI-MS/MS and identified by database search analysis using the Mascot search engine. The results revealed that low-temperature conditioning of garlic "seed" cloves causes alterations in the accumulation of proteins involved in different physiological processes such as cellular growth, antioxidative/oxidative state, macromolecules transport, protein folding and transcription regulation process. The metabolic pathways affected include protein biosynthesis and quality control system, photosynthesis, photorespiration, energy production, and carbohydrate and nucleotide metabolism. These processes can work cooperatively to establish a new cellular homeostasis that might be related with the physiological and biochemical changes observed in previous studies.

Profile of the contents of different forms of soybean isoflavones and the effect of germination time on these compounds and the physical parameters in soybean sprouts.

The aim of this study was to evaluate the profile of the contents of different forms of soybean isoflavones and the effect of germination time on these compounds and the physical parameters in BRS 284 soybean sprouts. Soybean seeds were germinated for 168 h, and the sprouts were collected every 24 h. The physical parameters and contents of different forms of isoflavones of the seeds and soybean sprouts were evaluated, and the data were subjected to regression analysis. The soybean seeds contained 26.0% ß-glucosides, 72.9% malonylglucosides and 1.2% aglycones. The yield of soybean sprouts was 632.4%. The effect of germination time was quadratic on the length, moisture and on the daidzin, genistin and genistein content; linear on the fresh weight and on the malonyldaidzin content. The dry matter and malonylglycitin content was constant, and glycitin and glycitein were not detected in the soybean sprouts.

Isoflavones of the soybean components and the effect of germination time in the cotyledons and embryonic axis.

The aim of this study was to evaluate the content of different forms of isoflavones of BRS 284 soybean components and the effect of germination time in the cotyledons, radicle, and hypocotyl. Seeds were germinated until 168 h at 35 °C and collected each 24 h. The isoflavone content was determined by ultraperformance liquid chromatography, and the data were subjected to regression analysis. In cotyledons, germination time had a quadratic effect on daidzin and genistin contents and a linear effect on malonyldaidzin and malonylgenistin contents. In radicles, germination time had a quadratic effect on daidzin, glycitin, malonylgenistin, and malonylglycitin contents in addition to a linear effect on malonyldaidzin content. In hypocotyls, germination time showed a cubic effect on daidzin and genistin contents, a quadratic effect on malonyldaidzin, malonylgenistin, and malonylglycitin contents, and a linear effect on genistein content; glycitin, daidzein and glycitein were detected in a few germination times.

Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts.

BACKGROUND: Broccoli sprouts are a good source of secondary metabolites, exhibiting biological activity, such as polyphenols, whose concentration is affected by the exposure to exogenous elicitors. The aim of this work was to investigate the effect of sodium selenate, chitosan and methyl jasmonate, applied directly to the seeds or through irrigation, on the content and profile of phenolic compounds in broccoli sprouts. The effect on antioxidant activity was also investigated. RESULTS: Methyl jasmonate and chitosan decreased antioxidant capacity. Methyl jasmonate significantly decreased total polyphenols content in comparison with control sprouts, while chitosan significantly increased it. Sodium selenate had no statistical effect on antioxidant capacity and total polyphenols concentration. The polyphenols profile in sprouts was composed by quercetin, morine, genisteine, luteoline and sinapic acid. Elicitor type and concentration affected the synthesis of these compounds. Chitosan stimulated the synthesis of quercetin, sinapic acid and morine, whereas methyl jasmonate stimulated the synthesis of luteoline. Sodium selenate had no effect on polyphenols synthesis. CONCLUSION: The exposure of broccoli to the elicitors produced changes in the phenolic compounds profile of broccoli sprouts. Besides, the stimulation of phenolic compounds synthesis was elicitor-specific, thus opening the possibility of managing culture conditions to increase the content of a specific phenolic compound.

Herbicidas e danos físicos em tocos de teca para controle de brotos após o desbaste / Herbicides and physical damages in teak stumps to control of sprouts after thinning

Tocos de árvores desbastadas de Tectona grandis apresentam rebrota intensa que compete com as árvores remanescentes. O objetivo deste trabalho foi avaliar a eficácia da aplicação isolada ou combinada de diferentes concentrações dos herbicidas picloram e triclopyr, associados ou não à aplicação de danos físicos, no controle dessas brotações. Em um povoamento com quatro anos de idade, os tocos foram tratados imediatamente após o desbaste. Em outro povoamento com seis anos, foram tratadas as brotações presentes nos tocos desbastados no ano anterior. Foi registrada a porcentagem de tocos mortos, o número de brotações por toco e as respectivas alturas. No primeiro ensaio, a aplicação combinada de picloram a 0,48% com triclopyr a 0,96%, associada a 20 rachas com machado matou todos os tocos. No segundo ensaio, a maior eficácia, 21,7%, foi registrada com roçada prévia das brotações e a aplicação de picloram a 0,96%. Após o desbaste, a aplicação isolada de picloram ou combinada com triclopyr associada ou não aos danos físicos é eficiente para controlar os brotos de teca. A aplicação nas rebrotas de tocos desbastados no ano anterior apresenta alguma eficiência, mas com menor percentual de tocos mortos em relação à aplicação após o desbaste.

The stumps of thinning trees of Tectona grandis L.f. present intense sprouts that compete with the remaining trees. The efficacy of the control of sprouts with the herbicides picloram and triclopyr, associated or no it applications of physical damages, were evaluated. Immediately after thinning, in plantation with four years old, the stumps were treated, and in other plantation, with age of six, the sprouts of stumps thinned in the previous year, were treated. The percentage of died stumps, the number of sprouts by stump and the respective heights were registered. In the first trial, the combined application of picloram at 0.48% with triclopyr at 0.96%, associated a 20 cracks with axe killed all stumps. In the second trial, the best efficacy, 21.7%, was obtained with the previous mowing of sprouts and application of picloram at 0.96%. After thinning, the isolated application of picloram or combined with triclopyr, associates or not a physical damage is efficient to control sprouts of teak. The application in the sprouts of stumps thinned in the last year present any efficiency, with least percentage of dead stumps in relation to application after thinning.

Herbicidas e danos físicos em tocos de teca para controle de brotos após o desbaste

The stumps of thinning trees of Tectona grandis L.f. present intense sprouts that compete with the remaining trees. The efficacy of the control of sprouts with the herbicides picloram and triclopyr, associated or no it applications of physical damages, were evaluated. Immediately after thinning, in plantation with four years old, the stumps were treated, and in other plantation, with age of six, the sprouts of stumps thinned in the previous year, were treated. The percentage of died stumps, the number of sprouts by stump and the respective heights were registered. In the first trial, the combined application of picloram at 0.48% with triclopyr at 0.96%, associated a 20 cracks with axe killed all stumps. In the second trial, the best efficacy, 21.7%, was obtained with the previous mowing of sprouts and application of picloram at 0.96%. After thinning, the isolated application of picloram or combined with triclopyr, associates or not a physical damage is efficient to control sprouts of teak. The application in the sprouts of stumps thinned in the last year present any efficiency, with least percentage of dead stumps in relation to application after thinning.

Tocos de árvores desbastadas de Tectona grandis apresentam rebrota intensa que compete com as árvores remanescentes. O objetivo deste trabalho foi avaliar a eficácia da aplicação isolada ou combinada de diferentes concentrações dos herbicidas picloram e triclopyr, associados ou não à aplicação de danos físicos, no controle dessas brotações. Em um povoamento com quatro anos de idade, os tocos foram tratados imediatamente após o desbaste. Em outro povoamento com seis anos, foram tratadas as brotações presentes nos tocos desbastados no ano anterior. Foi registrada a porcentagem de tocos mortos, o número de brotações por toco e as respectivas alturas. No primeiro ensaio, a aplicação combinada de picloram a 0,48% com triclopyr a 0,96%, associada a 20 rachas com machado matou todos os tocos. No segundo ensaio, a maior eficácia, 21,7%, foi registrada com roçada prévia das brotações e a aplicação de picloram a 0,96%. Após o desbaste, a aplicação isolada de picloram ou combinada com triclopyr associada ou não aos danos físicos é eficiente para controlar os brotos de teca. A aplicação nas rebrotas de tocos desbastados no ano anterior apresenta alguma eficiência, mas com menor percentual de tocos mortos em relação à aplicação após o desbaste.