ABSTRACT
Epilepsy, a chronic neurological disorder characterized by recurrent unprovoked seizures, presents a substantial challenge in approximately one-third of cases exhibiting resistance to conventional pharmacological treatments. This study investigated the effect of 4-allyl-2,6-dimethoxyphenol, a phenolic compound derived from various natural sources, in different models of induced seizures and its impact on animal electroencephalographic (EEG) recordings. Adult male Swiss albino mice were pre-treated (i.p.) with a dose curve of 4-allyl-2,6-dimethoxyphenol (50, 100, or 200â¯mg/kg), its vehicle (Tween), or standard antiepileptic drug (Diazepam; or Phenytoin). Subsequently, the mice were subjected to different seizure-inducing models - pentylenetetrazole (PTZ), 3-mercaptopropionic acid (3-MPA), pilocarpine (PILO), or maximal electroshock seizure (MES). EEG analysis was performed on other animals surgically implanted with electrodes to evaluate brain activity. Significant results revealed that animals treated with 4-allyl-2,6-dimethoxyphenol exhibited increased latency to the first myoclonic jerk in the PTZ and PILO models; prolonged latency to the first tonic-clonic seizure in the PTZ, 3-MPA, and PILO models; reduced total duration of tonic-clonic seizures in the PTZ and PILO models; decreased intensity of convulsive seizures in the PTZ and 3-MPA models; and diminished mortality in the 3-MPA, PILO, and MES models. EEG analysis indicated an increase in the percentage of total power attributed to beta waves following 4-allyl-2,6-dimethoxyphenol administration. Notably, the substance protected from behavioral and electrographic seizures in the PTZ model, preventing increases in the average amplitude of recording signals while also inducing an increase in the participation of theta and gamma waves. These findings suggest promising outcomes for the tested phenolic compound across diverse pre-clinical seizure models, highlighting the need for further comprehensive studies to elucidate its underlying mechanisms and validate its clinical relevance in epilepsy management.
Subject(s)
Anticonvulsants , Disease Models, Animal , Electroencephalography , Electroshock , Pentylenetetrazole , Seizures , Animals , Male , Seizures/drug therapy , Seizures/chemically induced , Seizures/physiopathology , Mice , Anticonvulsants/pharmacology , Pentylenetetrazole/toxicity , Electroencephalography/drug effects , Anisoles/pharmacology , Dose-Response Relationship, Drug , Pilocarpine/toxicity , Brain/drug effects , Brain/physiopathology , 3-Mercaptopropionic Acid/pharmacology , Convulsants/toxicityABSTRACT
Recently, solution-based surface-enhanced Raman scattering (SERS) detection technique has been widely recognized due to its cost-effectiveness, simplicity, and ease of use. However, solution-based SERS is limited for practical applications mainly because of the weak adsorption affinity of the target biomolecules to the surface of plasmonic nanoparticles. Herein, we developed a highly sensitive solution-based SERS sensing platform based on mercaptopropionic acid (MPA)-capped silver-coated gold nanostars (SGNS@MPA), which allows efficient enrichment on the nanostars surface for improved detection of an analyte: creatinine, a potential biomarker of chronic kidney disease (CKD). The SGNS@MPA exhibited high enrichment ability towards creatinine molecules in alkaline medium (pH-9) through multiple hydrogen bonding interaction, which causes aggregation of the nanoparticles and enhances the SERS signal of creatinine. The detection limit for creatinine was achieved at 0.1 nM, with a limit of detection (LOD) value of 14.6 pM. As a proof-of-concept demonstration, we conducted the first quantitative detection of creatinine in noninvasive human fluids, such as saliva and sweat, under separation-free conditions. We achieved a detection limit of up to 1 nM for both saliva and sweat, with LOD values as low as 0.136 nM for saliva and 0.266 nM for sweat. Overall, our molecular enrichment strategy offers a new way to improve the solution-based SERS detection technique for real-world practical applications in point-of-care settings and low-resource settings.
Subject(s)
Creatinine , Gold , Hydrogen Bonding , Metal Nanoparticles , Silver , Spectrum Analysis, Raman , Spectrum Analysis, Raman/methods , Gold/chemistry , Creatinine/analysis , Creatinine/chemistry , Metal Nanoparticles/chemistry , Humans , Silver/chemistry , Limit of Detection , Solutions , 3-Mercaptopropionic Acid/chemistry , Saliva/chemistryABSTRACT
This study explores the principles of resonance energy transfer and adsorption modulation using composites of Cu2S-MPA/NGODs. These composites can efficiently control the quenching process of electrochemiluminescence (ECL). Mercaptopropionic acid (MPA) was added during the synthesis of Cu2S-MPA to enhance its attachment to nitrogen-doped graphene quantum dots (NGODs). The UV absorption peaks of NGODs coincided with the emission peaks of luminol ECL, enabling resonance energy transfer and enhancing the quenching capability of Cu2S-MPA. Meanwhile, there is another quenching strategy. When the readily reducible Cu+ ions underwent partial reduction to Cu when they were bound to NGODs. This weakened the electrocatalytic effect on reactive oxygen species (ROS) and had a detrimental impact on electron transfer. Under optimal conditions, the immunosensor ECL intensity decreased linearly with the logarithm of carcinoembryonic antigen (CEA) concentration in the range of 0.00001-40 ng/mL, with a detection limit of 0.269 fg/mL. The sensor was effectively utilized for the identification of CEA in actual serum samples.
Subject(s)
Carcinoembryonic Antigen , Copper , Electrochemical Techniques , Graphite , Luminescent Measurements , Quantum Dots , Copper/chemistry , Quantum Dots/chemistry , Graphite/chemistry , Carcinoembryonic Antigen/blood , Carcinoembryonic Antigen/analysis , Luminescent Measurements/methods , Adsorption , Electrochemical Techniques/methods , Limit of Detection , 3-Mercaptopropionic Acid/chemistry , Humans , Energy Transfer , Biosensing Techniques/methods , SulfidesABSTRACT
3-mercaptopropionate (3MPA) dioxygenase (MDO) is a mononuclear nonheme iron enzyme that catalyzes the O2-dependent oxidation of thiol-bearing substrates to yield the corresponding sulfinic acid. MDO is a member of the cysteine dioxygenase family of small molecule thiol dioxygenases and thus shares a conserved sequence of active site residues (Serine-155, Histidine-157, and Tyrosine-159), collectively referred to as the SHY-motif. It has been demonstrated that these amino acids directly interact with the mononuclear Fe-site, influencing steady-state catalysis, catalytic efficiency, O2-binding, and substrate coordination. However, the underlying mechanism by which this is accomplished is poorly understood. Here, pulsed electron paramagnetic resonance spectroscopy [1H Mims electron nuclear double resonance spectroscopy] is applied to validate density functional theory computational models for the MDO Fe-site simultaneously coordinated by substrate and nitric oxide (NO), (3MPA/NO)-MDO. The enhanced resolution provided by electron nuclear double resonance spectroscopy allows for direct observation of Fe-bound substrate conformations and H-bond donation from Tyr159 to the Fe-bound NO ligand. Further inclusion of SHY-motif residues within the validated model reveals a distinct channel restricting movement of the Fe-bound NO-ligand. It has been argued that the iron-nitrosyl emulates the structure of potential Fe(III)-superoxide intermediates within the MDO catalytic cycle. While the merit of this assumption remains unconfirmed, the model reported here offers a framework to evaluate oxygen binding at the substrate-bound Fe-site and possible reaction mechanisms. It also underscores the significance of hydrogen bonding interactions within the enzymatic active site.
Subject(s)
Catalytic Domain , Dioxygenases , Models, Molecular , 3-Mercaptopropionic Acid/chemistry , Catalysis , Dioxygenases/chemistry , Dioxygenases/metabolism , Electron Spin Resonance Spectroscopy , Iron/metabolism , Nitric Oxide/metabolism , Oxygen/metabolism , Protein Structure, TertiaryABSTRACT
Biobased films were synthesized from starch oleate (DS = 2.2) cross-linked with polyethylene glycol with Mn = 2000 and 1000 g · mol-1, and ethylene glycol, all of which were esterified with either lipoic acid (LA) or 3-mercaptopropionic acid (MPA). Cross-linking was achieved through a UV-initiated thiol-ene click, and confirmed by Fourier transform infrared spectroscopy and rheometry. The films exhibit higher degradation temperatures, and an increased degree of crystallinity as cross-linker length increased. The introduction of MPA-based cross-linkers resulted in hydrophilic films, while the contact angle was barely affected by the addition of LA-based cross-linkers. A reduction in maximum strength upon introducing the cross-linkers was observed, while an increase in elongation was observed for most of the LA-based cross-linkers. Our results demonstrate the potential for tuning the mechanical and thermal properties of starch-based films through the cross-linker choice, with some formulations exhibiting increased flexibility that may be well suited for packaging applications.
Subject(s)
Starch , Sulfhydryl Compounds , Sulfhydryl Compounds/chemistry , Starch/chemistry , Oleic Acid , Polyethylene Glycols/chemistry , 3-Mercaptopropionic Acid/chemistryABSTRACT
Herein, we developed a class of functionalized silicon nanoparticles (F-SiNPs) bio-probes named thiol-conjugated F-SiNPs. They combine excellent biocompatibility with small dimensions (<10 nm) and biological usefulness with sustained and robust fluorescence (3.32% photoluminescent quantum yield). Identifying 3-Mercaptopropionic acid (3-MPA), which lowers the quantity of gamma-aminobutyric acid in the brain, and mercury (Hg2+) was a crucially important step since their excessive levels are a sign of several disorders. Using F-SiNPs as a fluorescent bio-probe, we provided an "off-on" technique for sensitively and selectively determining Hg2+ and 3-MPA in this study. The 3-(2-aminoethylamino) propyl (dimethoxymethylsilane) and basic fuchsin as precursors were hydrothermally treated to produce the F-SiNPs exhibiting green fluorescence. Our results suggest that Hg2+ reduced the fluorescence of F-SiNPs because of strong ionic interactions and metal-ligand binding among many thiols and carboxyl groupings at the surface of Hg2+ and F-SiNPs. Additionally, the resultants demonstrated that after being quenched by Hg2+, the produced F-SiNPs led to the distinctive "off-on" response to 3-MPA. Moreover, the method could detect Hg2+ and 3-MPA with limits of detection of 0.065 µM and 0.017 µM, respectively. The technique employed is quick, easy, affordable, and environmentally friendly. The sensing platform has successfully determined Hg2+ and 3-MPA in urine, water, and human serum samples.
Subject(s)
Mercury , Nanoparticles , Humans , Silicon , 3-Mercaptopropionic Acid , Fluorescent Dyes , Spectrometry, Fluorescence/methods , Sulfhydryl CompoundsABSTRACT
Procalcitonin (PCT) is considered a sepsis and infection biomarker. Herein, an interdigitated electrochemical immunosensor for the determination of PCT has been developed. The interdigitated electrode was made of the laser-engraved graphene electrode decorated with gold (LEGE/Aunano). The scanning electron microscopy indicated the LEGE/Aunano has been fabricated successfully. After that, the anti-PTC antibodies were immobilized on the surface of the electrode by using 3-mercaptopropionic acid. The electrochemical performance of the fabricated immunosensor was studied using electrochemical impedance spectroscopy (EIS). The EIS method was used for the determination of PCT in the concentration range of 2.5-800 pg/mL with a limit of detection of 0.36 pg/mL. The effect of several interfering agents such as the C reactive protein (CRP), immunoglobulin G (IgG), and human serum albumin (HSA) was also studied. The fabricated immunosensor had a good selectivity to the PCT. The stability of the immunosensor was also studied for 1 month. The relative standard deviation (RSD) was obtained to be 5.2%.
Subject(s)
Biosensing Techniques , Graphite , Metal Nanoparticles , Humans , Gold/chemistry , Graphite/chemistry , Procalcitonin , Biosensing Techniques/methods , Metal Nanoparticles/chemistry , C-Reactive Protein , 3-Mercaptopropionic Acid , Immunoassay/methods , Electrodes , Immunoglobulin G , Serum Albumin, Human , Electrochemical Techniques/methods , Limit of DetectionABSTRACT
In this study, the 3-mercaptopropionic acid (MA) was chosen to achieve the anionic intercalation into the green rust (GR) materials (MA-GR). The zeolite-rich tuff functionalized with the MA-intercalated GR (MA-GR-tuff) was subsequently synthesized and used to remove both HgII cations and CrVI anions in a binary system. MA-GR-tuff showed the best adsorption capacities to both HgII and CrVI among the adsorbent materials. The optimal combination of parameters was determined as the molar ratio of FeII to FeIII of 3.5, the molar ratio of OH- to the total iron of 3.75, the molar ratio of MA to the total iron of 2.5, and the mass ratio of the total iron to the tuff of 1.25. The pseudo-first-order kinetic model was appropriate in describing the kinetic sorption of CrVI by MA-GR-tuff. Both the pseudo-first-order kinetic model and Elovich were suitable for explaining HgII sorption. The maximum monolayer adsorption capacities of MA-GR-tuff towards CrVI and HgII were 185.19 mg/g and 72.99 mg/g, respectively. More flocs and plumes were formed in the MA-GR while the intercalation and more pores and crevices of different sizes were found in the MA-GR-tuff. Sulfhydryl complexation and the molecular sieve of tuff obviously both played a role in influencing the adsorption process. This study directly overcomes the drawback brought by the natural tuff to the treatment of a cationic-and-anionic binary system and supplies a new kind of tuff-based adsorbent for the potential use for the remediation of HM-contaminated wastewater.
Subject(s)
Mercury , Water Pollutants, Chemical , Zeolites , 3-Mercaptopropionic Acid , Ferric Compounds , Hydrogen-Ion Concentration , Chromium/analysis , Adsorption , Iron , Kinetics , Anions , Cations , Water Pollutants, Chemical/analysisABSTRACT
The aim of this work is to develop a reusable polypropylene glycol (PPG):ß-cyclodextrin (ßCD) biosensor for cortisol detection. To achieve the most stable support for ßCD, we developed two PPG surfaces. The first surface is based on a gold surface modified with SAM of 3-mercaptopropionic acid (3MPA), and the second surface is based on a glassy carbon surface grafted with 4-carboxyphenyl diazonium salt. We characterized both surfaces by EIS, XPS, and ATR-FTIR and evaluated the stability and reusability of each surface. We found the GC-carboxyphenyl-PPG:ßCD is stable for at least 1 month. We have also demonstrated the reusability of the surface up to 10 times. In detecting cortisol, we used a nonfaradaic electrochemical impedance capacitive model to interpret the surface confirmation changes. We achieved sensitive detection of cortisol in PBS buffer, urine, and saliva with limit of detection of 2.13, 1.29, and 1.33 nM, respectively.
Subject(s)
Biosensing Techniques , Cyclodextrins , beta-Cyclodextrins , 3-Mercaptopropionic Acid , Carbon/chemistry , Electrochemical Techniques , Electrodes , Gold/chemistry , HydrocortisoneABSTRACT
The reduction of vanadate (+V) in the presence of 3-mercaptopropionic acid was studied using a chromatographic method for the determination of vanadate (+V) versus vanadyl (+IV) species. Ion chromatography was combined with spectrophotometric investigation of the absorption properties of the solution. The chromatographic method for the separation of vanadate (+V) and vanadyl (+IV) was carried out with an anion exchange column. In the initial reaction mixture containing vanadate (+V) and 3-mercaptopropionic acid, ethylenediaminetetraacetic acid - EDTA was added in an excessive amount relative to the concentration of reactants in the solution. After the ligand exchange reaction, the added EDTA terminates the reduction, allowing redox speciation in the solutions. A strong pH dependence of the reduction rates in the investigated solution was observed. The vanadate reduction seems to proceed in 2 steps: 1) formation of the intermediate vanadate (+V)-thioester; 2) reduction reaction and formation of the vanadyl (+IV)-thiol complex. The obtained results strongly suggest that the reaction of vanadate (+V)-thioester formation is proton catalyzed. It was observed that the overall reduction rates are pH dependent due to the complex vanadate (+V) solution speciation and changes in the ionic form of 3-mercaptopropionic acid.
Subject(s)
3-Mercaptopropionic Acid , Vanadates , Chromatography , Edetic Acid , Oxidation-Reduction , Vanadates/chemistry , VanadiumABSTRACT
The resistance of melanoma to BRAF inhibitors remains a tough clinical challenge. In order to explore the underlying mechanism of drug resistance in melanoma, we established the resistant cell line to vemurafenib, and assessed the changes of drug-resistant cells on proliferation, apoptosis, oxidative stress and tumor stemness. Our results suggest that phosphoenolpyruvate carboxykinase1 (PCK1) is activated and inhibits the oxidative stress caused by vemurafenib in drug-resistant cells. Long term treatment of vemurafenib increases the expression of PCK1 which reduces the production of reactive oxygen species (ROS) by activating PI3K/Akt pathway. After the inhibition of PCK1 by 3-mercaptopropionic acid (3-MPA), the therapeutic sensitivity of vemurafenib is restored. In conclusion, this study disclosed that drug-resistant cells appeared to regulate their own proliferation, oxidative stress and tumor dryness by activating Akt/PCK1/ROS pathway, and shed new insights into acquiring drug resistance in melanoma.
Subject(s)
3-Mercaptopropionic Acid , Melanoma , Humans , Vemurafenib/pharmacology , 3-Mercaptopropionic Acid/pharmacology , 3-Mercaptopropionic Acid/therapeutic use , Phosphoenolpyruvate , Drug Resistance, Neoplasm , Proto-Oncogene Proteins c-akt/metabolism , Reactive Oxygen Species/metabolism , Phosphatidylinositol 3-Kinases , Indoles/pharmacology , Sulfonamides/pharmacology , Proto-Oncogene Proteins B-raf/genetics , Proto-Oncogene Proteins B-raf/metabolism , Melanoma/drug therapy , Melanoma/genetics , Melanoma/metabolism , Protein Kinase Inhibitors/pharmacology , Cell Line, TumorABSTRACT
Lignin is abundant and contains a large number of aromatic groups. Herein, CdxZn1-xS photocatalyst with tunable band gap energy was successfully synthesized by using 3-mercaptopropionic acid as a structure tuning additive. CdxZn1-xS can depolymerize alkaline lignin to vanillin by the photocatalytic process. Each gram of alkaline lignin can produce 46.5 mg of vanillin. 2-Phenoxy-1-phenylethanol (PP-ol) and other model compounds were used to understand the depolymerizing process of lignin. Fine tuned CdxZn1-xS can effectively cleave the Cß-O-4 bond existed in PP-ol under simulated sunlight. The highest conversion of PP-ol was 89.5% with phenol and acetophenone yields of 66.2% and 33.5%, respectively. The mechanism studies confirm that the Cα-H in PP-ol and lignin is firstly dehydrogenated to form Cα radical intermediates, and then the photogenerated electrons break the adjacent Cß-O bond. This research provides a new strategy to prepare valuable chemicals by virtue of renewable biomass and simulated sunlight.
Subject(s)
Benzaldehydes/chemistry , Lignin/chemistry , Sulfides/chemistry , 3-Mercaptopropionic Acid/chemistry , Cadmium Chloride/chemistry , Catalysis , Molecular Structure , Photochemical Processes , Polymerization , Zinc Acetate/chemistryABSTRACT
Herein, the quench model of the moving exchange boundary (MEB) was first created via a ligand of 5,5'-dithiobis(2-nitro-benzoic acid) (DTNB) and group of 3-mercaptopropionic acid (MPA) capped on QDs, and then the recovery model was formed via MPA and 2-nitro-5-thiobenzoic acid (TNB) capped on QDs. The theory on MEB dynamics and width was developed based on the two reversible models, the simulation was conducted for the illumination of MEB, and the protocol was described for the MEB runs. The experiments revealed that (i) the quench model could be created via DTNB and MPA capped on QDs and the recovery one could be in situ formed via MPA and TNB capped on QDs, showing the feasibility of MEB models; (ii) the simulations on MEB dynamics and width were in coincidence with the theoretic predictions, showing the validity of two models; and (iii) the experiments demonstrated the validity of models, predictions, and simulations. The models and theory have potential for development of a biosensor, nanoparticle characterization, separation science, and an affinity assay of ligand-QDs.
Subject(s)
Cadmium Compounds , Quantum Dots , 3-Mercaptopropionic Acid , Electrophoresis , LigandsABSTRACT
Thiol dioxygenases are a subset of nonheme iron oxygenases that catalyze the formation of sulfinic acids from sulfhydryl-containing substrates and dioxygen. Among this class, cysteine dioxygenases (CDOs) and 3-mercaptopropionic acid dioxygenases (3MDOs) are the best characterized, and the mode of substrate binding for CDOs is well understood. However, the manner in which 3-mercaptopropionic acid (3MPA) coordinates to the nonheme iron site in 3MDO remains a matter of debate. A model for bidentate 3MPA coordination at the 3MDO Fe-site has been proposed on the basis of computational docking, whereas steady-state kinetics and EPR spectroscopic measurements suggest a thiolate-only coordination of the substrate. To address this gap in knowledge, we determined the structure of Azobacter vinelandii 3MDO (Av3MDO) in complex with the substrate analog and competitive inhibitor, 3-hydroxypropionic acid (3HPA). The structure together with DFT computational modeling demonstrates that 3HPA and 3MPA associate with iron as chelate complexes with the substrate-carboxylate group forming an additional interaction with Arg168 and the thiol bound at the same position as in CDO. A chloride ligand was bound to iron in the coordination site assigned as the O2-binding site. Supporting HYSCORE spectroscopic experiments were performed on the (3MPA/NO)-bound Av3MDO iron nitrosyl (S = 3/2) site. In combination with spectroscopic simulations and optimized DFT models, this work provides an experimentally verified model of the Av3MDO enzyme-substrate complex, effectively resolving a debate in the literature regarding the preferred substrate-binding denticity. These results elegantly explain the observed 3MDO substrate specificity, but leave unanswered questions regarding the mechanism of substrate-gated reactivity with dioxygen.
Subject(s)
3-Mercaptopropionic Acid/metabolism , Azotobacter vinelandii/enzymology , Dioxygenases/chemistry , Dioxygenases/metabolism , Iron/chemistry , Iron/metabolism , 3-Mercaptopropionic Acid/chemistry , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Binding Sites , Catalytic Domain , Crystallography, X-Ray/methods , Kinetics , Models, Molecular , Substrate SpecificityABSTRACT
Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10-13 M with a wide detection range from 1.0 × 10-6 to 1.0 × 10-12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis.
Subject(s)
Carbon/chemistry , DNA, Bacterial/analysis , Ferric Compounds/chemistry , Mycobacterium tuberculosis/isolation & purification , 3-Mercaptopropionic Acid/chemistry , Biosensing Techniques , Cetrimonium/chemistry , Electrochemical Techniques , Electrodes , Mycobacterium tuberculosis/geneticsABSTRACT
Injectable hydrogels with pH-sensitive and self-healing properties have great application potential in the field of anti-cancer drug carriers. In this work, an injectable hydrogel is prepared using 4armPEG-benzaldehyde (4armPEGDA) and N-carboxyethyl chitosan (CEC) as a new drug carrier. The gelation time, equilibrium swelling rate, degradation time, and dynamic modulus of the injectable hydrogels can be adjusted by merely changing the concentration of 4armPEGDA. The volume of the hydrogel shrinks at pH 5.6 and expands at pH 7.4, which helps to control the release of anti-cancer drug. At pH 5.6, the hydrogels show a fast and substantial Dox release effect, which is five times higher than that at pH 7.4. In vitro cumulative drug release of all the hydrogels reached equilibrium on about the fourth day, and the hydrogel is completely degraded within five days, which contributes to the Dox-loaded hydrogel to further release the remaining Dox. Moreover, the Dox-loaded hydrogel shows a strong inhibitory effect on the growth of human hepatocellular carcinoma cells (HepG2). Finally, the anti-tumor model experiment in vivo demonstrated that the Dox-loaded hydrogel can significantly inhibit tumor growth within five days. Therefore, such injectable hydrogels are excellent carriers for the potential treatment of hepatocellular carcinoma.
Subject(s)
3-Mercaptopropionic Acid/chemistry , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/chemistry , Chitosan/analogs & derivatives , Chitosan/chemistry , Hydrogels/chemistry , Hydrogen-Ion Concentration , Polyethylene Glycols/chemistry , Animals , Antineoplastic Agents/chemical synthesis , Cell Line, Tumor , Disease Models, Animal , Doxorubicin/administration & dosage , Doxorubicin/chemistry , Drug Carriers , Drug Liberation , Humans , Mice , Molecular Structure , Xenograft Model Antitumor AssaysABSTRACT
The composition, orientation, and conformation of proteins in biomolecular coronas acquired by nanoparticles in biological media contribute to how they are identified by a cell. While numerous studies have investigated protein composition in biomolecular coronas, relatively little detail is known about how the nanoparticle surface influences the orientation and conformation of the proteins associated with them. We previously showed that the peripheral membrane protein cytochrome c adopts preferred poses relative to negatively charged 3-mercaptopropionic acid (MPA)-gold nanoparticles (AuNPs). Here, we employ molecular dynamics simulations and complementary experiments to establish that cytochrome c also assumes preferred poses upon association with nanoparticles functionalized with an uncharged ligand, specifically ω-(1-mercaptounde-11-cyl)hexa(ethylene glycol) (EG6). We find that the display of the EG6 ligands is sensitive to the curvature of the surface-and, consequently, the effective diameter of the nearly spherical nanoparticle core-which in turn affects the preferred poses of cytochrome c.
Subject(s)
Gold , Metal Nanoparticles , 3-Mercaptopropionic Acid , Cytochromes c , LigandsABSTRACT
Mercaptopropionic acid (MPA) capped CdTe quantum dots (MPA-CdTe QDs) were synthesized in aqueous medium by hydrothermal method, which modified by Fe3+ could be used as a fluorescent probe to detect ascorbic acid (AA). MPA-CdTe QDs fluorescence probe could be used as successive sensor for metal ions and AA with "on-off-on" process. The fluorescence of QDs was quenched after adding Fe3+ to MPA-CdTe QDs. Then, the fluorescence of the Fe3+@MPA-CdTe QDs can be sensitively turned on by AA to give an "on-off-on" fluorescence response according to the oxidation-reduction between Fe3+ and AA. There was a linear relationship between fluorescence intensity quenching value and the concentration of Fe3+ in the range of 2-10 µM since Fe3+ sensitively reacted with CdTe QDs. The linear detection range for AA was 0.1-1 µM with a limit of detection of 6.6 nM. The principle is proved by fluorescence emission spectroscopy, nuclear magnetic resonance spectroscopy. The proposed method is successfully used to detect the AA in human plasma sample.
Subject(s)
3-Mercaptopropionic Acid/chemistry , Ascorbic Acid/analysis , Cadmium Compounds/chemistry , Fluorescent Dyes/chemistry , Quantum Dots/chemistry , Tellurium/chemistry , Oxidation-Reduction , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform InfraredABSTRACT
In recent years, gold nanoclusters (AuNCs) have received considerable attention as optical transducers in chemo/biosensors. Herein, a facile and efficient assay for NO2- has been successfully developed based on the fluorescence quenching of AuNCs co-modified by bovine serum albumin and 3-mercaptopropionic acid (BSA/MPA-AuNCs). In the presence of NO2- under acidic conditions, Fe2+ can be readily oxidized and transformed to Fe3+, which can significantly suppress the fluorescence of BSA/MPA-AuNCs via non-radiative electron-transfer mechanism. The linear range and detection limit for this system were found to be 5-30 µM (r = 0.9975) and 0.7 µM, respectively. Other common anions and cations showed only very minor interference with the NO2- detection. Furthermore, the effectiveness of the proposed sensing strategy was validated by the demonstration of good performance in the determination of the amount of NO2- in ham samples, rendering it a powerful tool for the assessment of food security and water quality.
Subject(s)
Food Analysis/methods , Iron/chemistry , Nanostructures/chemistry , Nitrites/analysis , 3-Mercaptopropionic Acid/chemistry , Biosensing Techniques , Fluorescence , Fluorescent Dyes/chemistry , Food Analysis/instrumentation , Gold/chemistry , Limit of Detection , Nitrites/chemistry , Oxidation-Reduction , Pork Meat/analysis , Sensitivity and Specificity , Serum Albumin, Bovine/chemistry , Spectrometry, FluorescenceABSTRACT
In this work, a novel, selective and sensitive fluorescent probe (sulfur doped graphene quantum dots, SGQDs) was designed for real-time detection of quercetin in red wine samples. SGQDs were synthesized by pyrolyzing citric acid (CA) and 3-Mercaptopropionic acid (MPA) and characterized through advanced techniques. It was observed that fluorescence intensity of SGQDs could be substantially quenched by the addition of quercetin through inner filter effect (IFE) mechanism. Additionally, a visual color change (colorless to light yellow) was also noticed after addition of quercetin into a solution of SGQDs. The change in SGQDs fluorescence intensity with varying quercetin content revealed good linearity in the 0-50.0 µM range with regression coefficient of 0.9943 and a lowest detection limit of 0.006 µg/mL. To authenticate the real-time application of SGQDs as a potential fluorescent probe, red wine samples having different quercetin concentrations were used for quantitative analysis, after the optimization of several analytical parameters.