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1.
J Neurovirol ; 22(3): 327-35, 2016 06.
Article in English | MEDLINE | ID: mdl-26567012

ABSTRACT

Despite the recent advances in antiretroviral therapy, human immunodeficiency virus type 1 (HIV-1) remains a global health threat. HIV-1 affects the central nervous system by releasing viral proteins that trigger neuronal death and neuroinflammation, and promotes alterations known as HIV-associated neurocognitive disorders (HAND). This disorder is not fully understood, and no specific treatments are available. Recently, we demonstrated that the HIV-1 envelope protein gp120IIIB induces a functional upregulation of the α7-nicotinic acetylcholine receptor (α7) in neuronal cells. Furthermore, this upregulation promotes cell death that can be abrogated with receptor antagonists, suggesting that α7 may play an important role in the development of HAND. The partial duplication of the gene coding for the α7, known as CHRFAM7A, negatively regulates α7 expression but its role in HIV infection has not been studied. Hence, we studied both CHRNA7 and CHRFAM7A regulation patterns in various gp120IIIB in vitro conditions. In addition, we measured CHRNA7 and CHRFAM7A expression levels in postmortem brain samples from patients suffering from different stages of HAND. Our results demonstrate the induction of CHRNA7 expression accompanied by a significant downregulation of CHRFAM7A in neuronal cells when exposed to pathophysiological concentrations of gp120IIIB. Our results suggest a dysregulation of CHRFAM7A and CHRNA7 expressions in the basal ganglia from postmortem brain samples of HIV+ subjects and expand the current knowledge about the consequences of HIV infection in the brain.


Subject(s)
AIDS Dementia Complex/genetics , Brain/virology , HIV Envelope Protein gp120/genetics , HIV-1/genetics , Host-Pathogen Interactions , alpha7 Nicotinic Acetylcholine Receptor/genetics , AIDS Dementia Complex/metabolism , AIDS Dementia Complex/pathology , AIDS Dementia Complex/virology , Adult , Autopsy , Basal Ganglia/metabolism , Basal Ganglia/pathology , Basal Ganglia/virology , Brain/metabolism , Brain/pathology , Cell Death/drug effects , Cell Line, Tumor , Female , Gene Expression Regulation , HIV Envelope Protein gp120/metabolism , HIV Envelope Protein gp120/pharmacology , HIV-1/metabolism , HIV-1/pathogenicity , Humans , Male , Middle Aged , Neurons/cytology , Neurons/drug effects , Neurons/metabolism , Severity of Illness Index , Signal Transduction , alpha7 Nicotinic Acetylcholine Receptor/metabolism
2.
J Biol Chem ; 287(5): 3079-86, 2012 Jan 27.
Article in English | MEDLINE | ID: mdl-22084248

ABSTRACT

Approximately 30-50% of the >30 million HIV-infected subjects develop neurological complications ranging from mild symptoms to dementia. HIV does not infect neurons, and the molecular mechanisms behind HIV-associated neurocognitive decline are not understood. There are several hypotheses to explain the development of dementia in HIV(+) individuals, including neuroinflammation mediated by infected microglia and neuronal toxicity by HIV proteins. A key protein associated with the neurological complications of HIV, gp120, forms part of the viral envelope and can be found in the CSF of infected individuals. HIV-1-gp120 interacts with several receptors including CD4, CCR5, CXCR4, and nicotinic acetylcholine receptors (nAChRs). However, the role of nAChRs in HIV-associated neurocognitive disorder has not been investigated. We studied the effects of gp120(IIIB) on the expression and function of the nicotinic receptor α7 (α7-nAChR). Our results show that gp120, through activation of the CXCR4 chemokine receptor, induces a functional up-regulation of α7-nAChRs. Because α7-nAChRs have a high permeability to Ca(2+), we performed TUNEL staining to investigate the effects of receptor up-regulation on cell viability. Our data revealed an increase in cell death, which was blocked by the selective antagonist α-bungarotoxin. The in vitro data are supported by RT-PCR and Western blot analysis, confirming a remarkable up-regulation of the α7-nAChR in gp120-transgenic mice brains. Specifically, α7-nAChR up-regulation is observed in mouse striatum, a region severely affected in HIV(+) patients. In summary, CXCR4 activation induces up-regulation of α7-nAChR, causing cell death, suggesting that α7-nAChR is a previously unrecognized contributor to the neurotoxicity associated with HIV infection.


Subject(s)
AIDS Dementia Complex/metabolism , Corpus Striatum/metabolism , HIV Envelope Protein gp120/metabolism , HIV-1/metabolism , Nerve Tissue Proteins/metabolism , Receptors, CXCR4/metabolism , Receptors, Nicotinic/metabolism , AIDS Dementia Complex/genetics , Animals , Bungarotoxins/pharmacology , Cell Death/genetics , Corpus Striatum/virology , HIV Envelope Protein gp120/genetics , HIV-1/genetics , Humans , Mice , Mice, Transgenic , Nerve Tissue Proteins/genetics , Receptors, CXCR4/genetics , Receptors, Nicotinic/genetics , Up-Regulation/drug effects , Up-Regulation/genetics , alpha7 Nicotinic Acetylcholine Receptor
3.
J Neurovirol ; 10 Suppl 1: 74-81, 2004.
Article in English | MEDLINE | ID: mdl-14982743

ABSTRACT

The emergence of a subset of circulating monocytes during human immunodeficiency virus type 1 (HIV-1) disease has been shown to correlate with cognitive impairment. Thus, it is hypothesized that diagnostic protein profiles may be obtained from these cells from patients with or at risk for HIV-1-associated dementia (HAD). To address this possibility, we used ProteinChip assays to define a unique monocyte-derived macrophage (MDM) protein fingerprint during HAD and whether it is affected by highly active antiretroviral therapy (HAART). The study included five Hispanic women, one with HAD, two HIV-1-infected without cognitive impairment, and two seronegative controls. All patients were matched by age and immune status. Monocytes were recovered from the peripheral blood leukocytes by Percoll gradient centrifugation and allowed to differentiate in vitro for 7 days. Cell lysates and supernatants were collected from the MDM and analyzed by surface enhanced laser desorption/ionization-time of flight ProteinChip assays. Seven unique protein peaks between 3.0 and 20.0 kDa were found in the HAD MDM sample. Each of these proteins were abrogated after HAART. Additional studies extending this one time point determination would serve to confirm the general utility of MDM protein profiling for the diagnosis and monitoring of HAD.


Subject(s)
AIDS Dementia Complex/genetics , AIDS Dementia Complex/immunology , HIV-1 , Macrophages/physiology , Macrophages/virology , Protein Array Analysis , AIDS Dementia Complex/diagnosis , Adult , Biomarkers , Female , Humans , Macrophages/cytology , Middle Aged , Monocytes/cytology , Proteomics
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