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1.
J Cell Biol ; 223(8)2024 Aug 05.
Article in English | MEDLINE | ID: mdl-38913027

ABSTRACT

Numerous biomedically important cargoes depend on adaptor protein complex-1 (AP-1) for their localization. However, controversy surrounds whether AP-1 mediates traffic from or to the Golgi. Robinson et al. (https://www.doi.org/10.1083/jcb.202310071) present compelling evidence that AP-1 mediates recycling to the Golgi.


Subject(s)
Adaptor Protein Complex 1 , Golgi Apparatus , Protein Transport , Golgi Apparatus/metabolism , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex 1/genetics , Humans , Kinetics , Animals
2.
Mol Biol Cell ; 35(5): ar61, 2024 May 01.
Article in English | MEDLINE | ID: mdl-38446634

ABSTRACT

Neurons are polarized cells that require accurate membrane trafficking to maintain distinct protein complements at dendritic and axonal membranes. The Kinesin-3 family members KIF13A and KIF13B are thought to mediate dendrite-selective transport, but the mechanism by which they are recruited to polarized vesicles and the differences in the specific trafficking role of each KIF13 have not been defined. We performed live-cell imaging in cultured hippocampal neurons and found that KIF13A is a dedicated dendrite-selective kinesin. KIF13B confers two different transport modes, dendrite- and axon-selective transport. Both KIF13s are maintained at the trans-Golgi network by interactions with the heterotetrameric adaptor protein complex AP-1. Interference with KIF13 binding to AP-1 resulted in disruptions to both dendrite- and axon-selective trafficking. We propose that AP-1 is the molecular link between the sorting of polarized cargoes into vesicles and the recruitment of kinesins that confer polarized transport.


Subject(s)
Adaptor Protein Complex 1 , Golgi Apparatus , Kinesins , trans-Golgi Network , Cells, Cultured , Golgi Apparatus/metabolism , Kinesins/metabolism , Neurons/metabolism , Protein Transport/genetics , Protein Transport/physiology , Adaptor Protein Complex 1/metabolism , trans-Golgi Network/metabolism
3.
J Biol Chem ; 300(3): 105700, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38307383

ABSTRACT

Selective retrograde transport from endosomes back to the trans-Golgi network (TGN) is important for maintaining protein homeostasis, recycling receptors, and returning molecules that were transported to the wrong compartments. Two important transmembrane proteins directed to this pathway are the Cation-Independent Mannose-6-phosphate receptor (CI-MPR) and the ATP7B copper transporter. Among CI-MPR functions is the delivery of acid hydrolases to lysosomes, while ATP7B facilitates the transport of cytosolic copper ions into organelles or the extracellular space. Precise subcellular localization of CI-MPR and ATP7B is essential for the proper functioning of these proteins. This study shows that both CI-MPR and ATP7B interact with a variant of the clathrin adaptor 1 (AP-1) complex that contains a specific isoform of the γ-adaptin subunit called γ2. Through synchronized anterograde trafficking and cell-surface uptake assays, we demonstrated that AP-1γ2 is dispensable for ATP7B and CI-MPR exit from the TGN while being critically required for ATP7B and CI-MPR retrieval from endosomes to the TGN. Moreover, AP-1γ2 depletion leads to the retention of endocytosed CI-MPR in endosomes enriched in retromer complex subunits. These data underscore the importance of AP-1γ2 as a key component in the sorting and trafficking machinery of CI-MPR and ATP7B, highlighting its essential role in the transport of proteins from endosomes.


Subject(s)
Adaptor Protein Complex 1 , Copper-Transporting ATPases , Endosomes , Protein Transport , Receptor, IGF Type 2 , trans-Golgi Network , Humans , Endosomes/metabolism , HeLa Cells , Protein Transport/genetics , Receptor, IGF Type 2/genetics , Receptor, IGF Type 2/metabolism , trans-Golgi Network/genetics , trans-Golgi Network/metabolism , Copper-Transporting ATPases/genetics , Copper-Transporting ATPases/metabolism , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex gamma Subunits/metabolism
4.
J Cell Biol ; 223(3)2024 03 04.
Article in English | MEDLINE | ID: mdl-38240799

ABSTRACT

The highly conserved HEATR5 proteins are best known for their roles in membrane traffic mediated by the adaptor protein complex-1 (AP1). HEATR5 proteins rely on fast-evolving cofactors to bind to AP1. However, how HEATR5 proteins interact with these cofactors is unknown. Here, we report that the budding yeast HEATR5 protein, Laa1, functions in two biochemically distinct complexes. These complexes are defined by a pair of mutually exclusive Laa1-binding proteins, Laa2 and the previously uncharacterized Lft1/Yml037c. Despite limited sequence similarity, biochemical analysis and structure predictions indicate that Lft1 and Laa2 bind Laa1 via structurally similar mechanisms. Both Laa1 complexes function in intra-Golgi recycling. However, only the Laa2-Laa1 complex binds to AP1 and contributes to its localization. Finally, structure predictions indicate that human HEATR5 proteins bind to a pair of fast-evolving interacting partners via a mechanism similar to that observed in yeast. These results reveal mechanistic insight into how HEATR5 proteins bind their cofactors and indicate that Laa1 performs functions besides recruiting AP1.


Subject(s)
Adaptor Proteins, Signal Transducing , Golgi Apparatus , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae , Humans , Adaptor Protein Complex 1/metabolism , Carrier Proteins/metabolism , Golgi Apparatus/metabolism , Saccharomyces cerevisiae/metabolism , Adaptor Proteins, Signal Transducing/genetics , Adaptor Proteins, Signal Transducing/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
5.
Oncogene ; 43(2): 92-105, 2024 Jan.
Article in English | MEDLINE | ID: mdl-37952080

ABSTRACT

Several studies have demonstrated the role of the oncogenic mutant p53 in promoting tumor progression; however, there is limited information on the effects of secreted oncogenic mutant p53 on the tumor microenvironment and tumor immune escape. In this study, we found that secretion of mutant p53, determined by exosome content, is dependent on its N-terminal dileucine motif via its binding to ß-adaptin, and inhibited by the CHK2-mediated-Ser 20 phosphorylation. Moreover, we observed that the mutant p53 caused downregulation and dysfunction of CD4+ T lymphocytes in vivo and downregulated the levels and activities of rate-limiting glycolytic enzymes in vitro. Furthermore, inhibition of mutant p53 secretion by knocking down AP1B1 or mutation of dileucine motif could reverse the quantity and function of CD4+ T lymphocytes and restrain the tumor growth. Our study demonstrates that the tumor-derived exosome-mediated secretion of oncogenic mutant p53 inhibits glycolysis to alter the immune microenvironment via functional suppression of CD4+ T cells, which may be the underlying mechanism for tumor immune escape. Therefore, targeting TDE-mediated p53 secretion may serve as a potential therapeutic target for cancer treatment.


Subject(s)
Neoplasms , Tumor Suppressor Protein p53 , Humans , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , Tumor Microenvironment/genetics , T-Lymphocytes/metabolism , Mutation , Neoplasms/genetics , Cell Line, Tumor , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex beta Subunits/genetics , Adaptor Protein Complex beta Subunits/metabolism
6.
Sci Rep ; 13(1): 15059, 2023 09 12.
Article in English | MEDLINE | ID: mdl-37700003

ABSTRACT

Despite being significant in various diseases, including cancers, the impact of copper metabolism on osteosarcoma (OS) remains largely unexplored. This study aimed to use bioinformatics analyses to identify a reliable copper metabolism signature that could improve OS patient prognosis prediction, immune landscape understanding, and drug sensitivity. Through nonnegative matrix factorization (NMF) clustering, we revealed distinct prognosis-associated clusters of OS patients based on copper metabolism-related genes (CMRGs), showing differential gene expression linked to immune processes. The risk model, comprising 13 prognostic CMRGs, was established using least absolute shrinkage and selection operator (LASSO) Cox regression, closely associated with the OS microenvironment's immune situation and drug sensitivity. Furthermore, we developed an integrated nomogram, combining the risk score and clinical traits to quantitatively predict OS patient prognosis. The calibration plot, timeROC, and timeROC analyses demonstrated its predictable accuracy and clinical usefulness. Finally, we identified three independent prognostic signatures for OS patients: COX11, AP1B1, and ABCB6. This study confirmed the involvement of CMRGs in OS patient prognosis, immune processes, and drug sensitivity, suggesting their potential as promising prognostic signatures and therapeutic targets for OS.


Subject(s)
Bone Neoplasms , Osteosarcoma , Humans , Copper , Osteosarcoma/genetics , Prognosis , Nomograms , Bone Neoplasms/genetics , Tumor Microenvironment/genetics , Adaptor Protein Complex 1 , Adaptor Protein Complex beta Subunits
7.
Pediatr Dev Pathol ; 26(4): 406-410, 2023.
Article in English | MEDLINE | ID: mdl-37278357

ABSTRACT

Inherited syndromes of congenital enteropathy are rare, with many genetic causes described. Mutations of the AP1S1 gene results in the syndrome of intellectual disability, enteropathy, deafness, peripheral neuropathy, ichthyosis, and keratoderma (IDEDNIK, formerly in the medical literature as MEDNIK). The clinicopathologic features of the enteropathy in IDEDNIK syndrome have not been fully explored. We describe a female infant who presented with metabolic acidosis, lethargy, and 14 watery stools per day. In the intensive care unit she required parenteral nutrition. She was found to have a novel homozygous pathogenic variant in the AP1S1 gene c.186T>G (p.Y62*). Esophagogastroduodenoscopy and colonoscopy at 6 months of age were grossly normal. However, histologic sections of the duodenum showed mild villous blunting and enterocytes with cytoplasmic vacuoles. CD10 immunostaining highlighted the disrupted brush border. MOC31 immunostaining was wild-type with a membranous pattern of expression. Electron microscopy of the duodenum showed scattered enterocytes cells with shortened and disrupted apical microvilli. Although there is a mixed gap diarrhea and disrupted brush border, there are no significant inclusions typical of microvillus inclusion disease, nor tufted enterocytes typical of tufting enteropathy, making the clinical and histopathologic features for this syndrome unique.


Subject(s)
Adaptor Protein Complex sigma Subunits , Malabsorption Syndromes , Female , Humans , Infant , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex sigma Subunits/genetics , Diarrhea/genetics , Duodenum , Malabsorption Syndromes/diagnosis , Malabsorption Syndromes/genetics , Malabsorption Syndromes/metabolism , Mutation , Syndrome
8.
J Biol Chem ; 299(3): 102979, 2023 03.
Article in English | MEDLINE | ID: mdl-36739948

ABSTRACT

The epidermal growth factor receptor (EGFR) plays important roles in cancer progression and is one of the major drug targets for targeted cancer therapy. Although fundamentally important, how newly synthesized EGFR is delivered to the cell surface to perform its cellular functions remains to be further investigated. In this study, we found using the approaches of gene knockout, siRNA knockdown, streptavidin pull-down, and co-immunoprecipitation assays that the clathrin adaptor complex-1 (AP-1) and Rab12 interact with EGFR and regulate the export of EGFR out of the trans-Golgi network (TGN). In addition, the tyrosine residue at the 998 position on human EGFR is critical to bind to AP-1, and this residue is important for TGN export of EGFR. We demonstrate that AP-1 and Rab12 are important for epidermal growth factor-induced phosphorylation of EGFR, cell elongation, and proliferation, suggesting that AP-1-mediated and Rab12-mediated post-Golgi trafficking is important for EGFR signaling. Moreover, TGN export of the constitutively activated mutant form of EGFR (EGFRL858R) is independent of AP-1 and Rab12. Our results reveal insights into the molecular mechanisms that mediate the TGN-to-cell surface delivery of EGFR and indicate that TGN export of WT EGFR and EGFRL858R depends on different cellular factors.


Subject(s)
Adaptor Protein Complex 1 , rab GTP-Binding Proteins , Humans , ErbB Receptors/genetics , ErbB Receptors/metabolism , Golgi Apparatus/metabolism , Protein Transport , rab GTP-Binding Proteins/genetics , rab GTP-Binding Proteins/metabolism , trans-Golgi Network/genetics , trans-Golgi Network/metabolism , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex 1/metabolism
9.
J Gerontol A Biol Sci Med Sci ; 78(3): 494-503, 2023 03 01.
Article in English | MEDLINE | ID: mdl-35512265

ABSTRACT

BACKGROUND: Motor resilience proteins have not been identified. This proteome-wide discovery study sought to identify proteins that may provide motor resilience. METHODS: We studied the brains of older decedents with annual motor testing, postmortem brain pathologies, and proteome-wide data. Parkinsonism was assessed using 26 items of a modified United Parkinson Disease Rating Scale. We used linear mixed-effect models to isolate motor resilience, defined as the person-specific estimate of progressive parkinsonism after controlling for age, sex, and 10 brain pathologies. A total of 8 356 high-abundance proteins were quantified from dorsal lateral prefrontal cortex using tandem mass tag and liquid chromatography-mass spectrometry. RESULTS: There were 391 older adults (70% female), mean age 80 years at baseline and 89 years at death. Five proteins were associated with motor resilience: A higher level of AP1B1 (Estimate -0.504, SE 0.121, p = 3.12 × 10-5) and GNG3 (Estimate -0.276, SE 0.068, p = 4.82 × 10-5) was associated with slower progressive parkinsonism. By contrast, a higher level of TTC38 (Estimate 0.140, SE 0.029, p = 1.87 × 10-6), CARKD (Estimate 0.413, SE 0.100, p = 3.50 × 10-5), and ABHD14B (Estimate 0.175, SE 0.044, p = 6.48 × 10-5) was associated with faster progressive parkinsonism. Together, these 5 proteins accounted for almost 25% of the variance of progressive parkinsonism above the 17% accounted for by 10 indices of brain pathologies. DISCUSSION: Cortical proteins may provide more or less motor resilience in older adults. These proteins are high-value therapeutic targets for drug discovery that may lead to interventions that maintain motor function despite the accumulation of as yet untreatable brain pathologies.


Subject(s)
Parkinson Disease , Parkinsonian Disorders , Humans , Female , Aged , Aged, 80 and over , Male , Proteome , Parkinson Disease/complications , Parkinsonian Disorders/complications , Brain/pathology , Prefrontal Cortex , Adaptor Protein Complex 1 , Adaptor Protein Complex beta Subunits
10.
Sci Signal ; 15(759): eadf6187, 2022 11 08.
Article in English | MEDLINE | ID: mdl-36346839
11.
Int J Mol Sci ; 23(19)2022 Sep 20.
Article in English | MEDLINE | ID: mdl-36232302

ABSTRACT

We assess the performance of mRNA capture sequencing to identify fusion transcripts in FFPE tissue of different sarcoma types, followed by RT-qPCR confirmation. To validate our workflow, six positive control tumors with a specific chromosomal rearrangement were analyzed using the TruSight RNA Pan-Cancer Panel. Fusion transcript calling by FusionCatcher confirmed these aberrations and enabled the identification of both fusion gene partners and breakpoints. Next, whole-transcriptome TruSeq RNA Exome sequencing was applied to 17 fusion gene-negative alveolar rhabdomyosarcoma (ARMS) or undifferentiated round cell sarcoma (URCS) tumors, for whom fluorescence in situ hybridization (FISH) did not identify the classical pathognomonic rearrangements. For six patients, a pathognomonic fusion transcript was readily detected, i.e., PAX3-FOXO1 in two ARMS patients, and EWSR1-FLI1, EWSR1-ERG, or EWSR1-NFATC2 in four URCS patients. For the 11 remaining patients, 11 newly identified fusion transcripts were confirmed by RT-qPCR, including COPS3-TOM1L2, NCOA1-DTNB, WWTR1-LINC01986, PLAA-MOB3B, AP1B1-CHEK2, and BRD4-LEUTX fusion transcripts in ARMS patients. Additionally, recurrently detected secondary fusion transcripts in patients diagnosed with EWSR1-NFATC2-positive sarcoma were confirmed (COPS4-TBC1D9, PICALM-SYTL2, SMG6-VPS53, and UBE2F-ALS2). In conclusion, this study shows that mRNA capture sequencing enhances the detection rate of pathognomonic fusions and enables the identification of novel and secondary fusion transcripts in sarcomas.


Subject(s)
Sarcoma , Soft Tissue Neoplasms , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex beta Subunits , Cell Cycle Proteins/genetics , Dithionitrobenzoic Acid , Humans , In Situ Hybridization, Fluorescence , Nuclear Proteins/genetics , Oncogene Proteins, Fusion/genetics , RNA , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sarcoma/diagnosis , Sarcoma/genetics , Sarcoma/pathology , Soft Tissue Neoplasms/pathology , Transcription Factors/genetics
12.
Nature ; 610(7933): 761-767, 2022 10.
Article in English | MEDLINE | ID: mdl-36261523

ABSTRACT

Stimulator of interferon genes (STING) functions downstream of cyclic GMP-AMP synthase in DNA sensing or as a direct receptor for bacterial cyclic dinucleotides and small molecules to activate immunity during infection, cancer and immunotherapy1-10. Precise regulation of STING is essential to ensure balanced immune responses and prevent detrimental autoinflammation11-16. After activation, STING, a transmembrane protein, traffics from the endoplasmic reticulum to the Golgi, where its phosphorylation by the protein kinase TBK1 enables signal transduction17-20. The mechanism that ends STING signalling at the Golgi remains unknown. Here we show that adaptor protein complex 1 (AP-1) controls the termination of STING-dependent immune activation. We find that AP-1 sorts phosphorylated STING into clathrin-coated transport vesicles for delivery to the endolysosomal system, where STING is degraded21. We identify a highly conserved dileucine motif in the cytosolic C-terminal tail (CTT) of STING that, together with TBK1-dependent CTT phosphorylation, dictates the AP-1 engagement of STING. A cryo-electron microscopy structure of AP-1 in complex with phosphorylated STING explains the enhanced recognition of TBK1-activated STING. We show that suppression of AP-1 exacerbates STING-induced immune responses. Our results reveal a structural mechanism of negative regulation of STING and establish that the initiation of signalling is inextricably associated with its termination to enable transient activation of immunity.


Subject(s)
Adaptor Protein Complex 1 , Clathrin , Adaptor Protein Complex 1/chemistry , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex 1/ultrastructure , Clathrin/metabolism , Cryoelectron Microscopy , DNA/metabolism , Immunity, Innate , Protein Serine-Threonine Kinases , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Membrane Proteins/ultrastructure , Amino Acid Motifs , Endosomes/metabolism , Lysosomes/metabolism , Phosphorylation
13.
Int J Mol Sci ; 23(17)2022 Sep 02.
Article in English | MEDLINE | ID: mdl-36077416

ABSTRACT

Neurofibromatosis type 2 is an autosomal dominant tumor-prone disorder mainly caused by NF2 point mutations or intragenic deletions. Few individuals with a complex phenotype and 22q12 microdeletions have been described. The 22q12 microdeletions' pathogenic effects at the genetic and epigenetic levels are currently unknown. We here report on 22q12 microdeletions' characterization in three NF2 patients with different phenotype complexities. A possible effect of the position was investigated by in silico analysis of 22q12 topologically associated domains (TADs) and regulatory elements, and by expression analysis of 12 genes flanking patients' deletions. A 147 Kb microdeletion was identified in the patient with the mildest phenotype, while two large deletions of 561 Kb and 1.8 Mb were found in the other two patients, showing a more severe symptomatology. The last two patients displayed intellectual disability, possibly related to AP1B1 gene deletion. The microdeletions change from one to five TADs, and the 22q12 chromatin regulatory landscape, according to the altered expression levels of four deletion-flanking genes, including PIK3IP1, are likely associated with an early ischemic event occurring in the patient with the largest deletion. Our results suggest that the identification of the deletion extent can provide prognostic markers, predictive of NF2 phenotypes, and potential therapeutic targets, thus overall improving patient management.


Subject(s)
Intellectual Disability , Neurofibromatosis 2 , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex beta Subunits , Humans , Intellectual Disability/genetics , Neurofibromatosis 2/genetics , Phenotype
14.
Mol Biol Cell ; 33(12): ar109, 2022 10 01.
Article in English | MEDLINE | ID: mdl-35976721

ABSTRACT

Adaptor protein 4 (AP-4) is a heterotetrameric complex composed of ε, ß4, µ4, and σ4 subunits that mediates export of a subset of transmembrane cargos, including autophagy protein 9A (ATG9A), from the trans-Golgi network (TGN). AP-4 has received particular attention in recent years because mutations in any of its subunits cause a complicated form of hereditary spastic paraplegia referred to as "AP-4-deficiency syndrome." The identification of proteins that interact with AP-4 has shed light on the mechanisms of AP-4-dependent cargo sorting and distribution within the cell. However, the mechanisms by which the AP-4 complex itself is assembled have remained unknown. Here, we report that the alpha- and gamma-adaptin-binding protein (AAGAB, also known as p34) binds to and stabilizes the AP-4 ε and σ4 subunits, thus promoting complex assembly. The physiological importance of these interactions is underscored by the observation that AAGAB-knockout cells exhibit reduced levels of AP-4 subunits and accumulation of ATG9A at the TGN like those in cells with mutations in AP-4-subunit genes. These findings demonstrate that AP-4 assembly is not spontaneous but AAGAB-assisted, further contributing to the understanding of an adaptor protein complex that is critically involved in development of the central nervous system.


Subject(s)
Adaptor Protein Complex Subunits , Membrane Proteins , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex 2/metabolism , Adaptor Protein Complex Subunits/metabolism , Adaptor Protein Complex gamma Subunits/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Adaptor Proteins, Vesicular Transport/metabolism , Membrane Proteins/metabolism , Protein Transport , trans-Golgi Network/metabolism
15.
Methods Mol Biol ; 2473: 195-212, 2022.
Article in English | MEDLINE | ID: mdl-35819768

ABSTRACT

Clathrin-coated vesicles mediate membrane cargo transportation from the plasma membrane, the trans-Golgi network, the endosome, and the lysosome. Heterotetrameric adaptor complexes 1 and 2 (AP1 and AP2) are bridges that link cargo-loaded membranes to clathrin coats. Assembly of AP2 was previously considered to be spontaneous; however, a recent study found AP2 assembly is a highly orchestrated process controlled by alpha and gamma adaptin binding protein (AAGAB). Evidence shows that AAGAB controls AP1 assembly in a similar way. Insights into the orchestrated assembly process and three-dimensional structures of assembly intermediates are only emerging. Here, we describe a protocol for reconstitution and purification of the complexes containing AAGAB and AP1 or AP2 subunits, known as AP1 and AP2 hemicomplexes. Our purification routinely yields milligrams of pure complexes suitable for structural analysis by X-ray crystallography and electron microscopy.


Subject(s)
Adaptor Protein Complex 2 , Adaptor Proteins, Vesicular Transport , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex 2/metabolism , Adaptor Proteins, Vesicular Transport/metabolism , Clathrin/metabolism , Clathrin-Coated Vesicles/metabolism
16.
Mol Cell Biol ; 42(7): e0007122, 2022 07 21.
Article in English | MEDLINE | ID: mdl-35727026

ABSTRACT

Host cell membrane-trafficking pathways are often manipulated by bacterial pathogens to gain cell entry, avoid immune responses, or to obtain nutrients. The 1,369-residue OtDUB protein from the obligate intracellular human pathogen Orientia tsutsugamushi bears a deubiquitylase (DUB) and additional domains. Here we show that OtDUB ectopic expression disrupts membrane trafficking through multiple mechanisms. OtDUB binds directly to the clathrin adaptor-protein (AP) complexes AP-1 and AP-2, and the OtDUB275-675 fragment is sufficient for binding to either complex. To assess the impact of OtDUB interactions with AP-1 and AP-2, we examined trans-Golgi trafficking and endocytosis, respectively. Endocytosis is reduced by two separate OtDUB fragments: one contains the AP-binding domain (OtDUB1-675), and the other does not (OtDUB675-1369). OtDUB1-675 disruption of endocytosis requires its ubiquitin-binding capabilities. OtDUB675-1369 also fragments trans- and cis-Golgi structures. Using a growth-based selection in yeast, we identified viable OtDUB675-1369 point mutants that also no longer caused Golgi defects in human cells. In parallel, we found OtDUB675-1369 binds directly to phosphatidylserine, and this lipid binding is lost in the same mutants. Together these results show that OtDUB contains multiple activities capable of modulating membrane trafficking. We discuss how these activities may contribute to Orientia infections.


Subject(s)
Orientia tsutsugamushi , Adaptor Protein Complex 1/metabolism , Adaptor Protein Complex 2/metabolism , Endocytosis , Golgi Apparatus/metabolism , Host-Pathogen Interactions , Humans , Orientia tsutsugamushi/metabolism , Protein Binding , Scrub Typhus/metabolism , Scrub Typhus/microbiology , Scrub Typhus/pathology
17.
Mol Biol Cell ; 33(9): ar80, 2022 08 01.
Article in English | MEDLINE | ID: mdl-35609212

ABSTRACT

Intracellular trafficking regulates the distribution of transmembrane proteins including the key determinants of epithelial polarity and adhesion. The Adaptor Protein 1 (AP-1) complex is the key regulator of vesicle sorting, which binds many specific cargoes. We examined roles of the AP-1 complex in epithelial morphogenesis, using the Drosophila wing as a paradigm. We found that AP-1 knockdown leads to ectopic tissue folding, which is consistent with the observed defects in integrin targeting to the basal cell-extracellular matrix adhesion sites. This occurs concurrently with an integrin-independent induction of cell death, which counteracts elevated proliferation and prevents hyperplasia. We discovered a distinct pool of AP-1 that localizes at the subapical adherens junctions. Upon AP-1 knockdown, E-cadherin is hyperinternalized from these junctions and becomes enriched at the Golgi and recycling endosomes. We then provide evidence that E-cadherin hyperinternalization acts upstream of cell death in a potential tumor-suppressive mechanism. Simultaneously, cells compensate for elevated internalization of E-cadherin by increasing its expression to maintain cell-cell adhesion.


Subject(s)
Adaptor Protein Complex 1 , Transcription Factor AP-1 , Adaptor Protein Complex 1/metabolism , Adherens Junctions/metabolism , Animals , Cadherins/metabolism , Cell Polarity , Drosophila/metabolism , Epithelial Cells/metabolism , Integrins/metabolism , Morphogenesis/physiology , Protein Transport/physiology , Transcription Factor AP-1/metabolism
18.
Curr Opin Cell Biol ; 76: 102079, 2022 06.
Article in English | MEDLINE | ID: mdl-35429729

ABSTRACT

The clathrin adaptor protein complex-1 (AP-1) is a central player in cell physiology and human health. It is best known for its role in linking clathrin to its cargo at the trans-Golgi network and endosomes. It participates in traffic important for the correct function of a large number of organelles, including the trans-Golgi network, endosomes, lysosomes, lysosome-related organelles, and plasma membrane. Although it was one of the first clathrin adaptors identified, new discoveries about cargo and pathways that depend on AP-1 continue to emerge. This review summarizes new research into AP-1 that further illuminates its roles in the traffic of plasma membrane proteins, in maintaining TGN content, and in human disease.


Subject(s)
Adaptor Proteins, Vesicular Transport , Transcription Factor AP-1 , Adaptor Protein Complex 1/metabolism , Adaptor Proteins, Vesicular Transport/metabolism , Clathrin/metabolism , Endosomes/metabolism , Humans , Protein Transport , Transcription Factor AP-1/metabolism , trans-Golgi Network/metabolism
19.
Eur J Med Genet ; 65(3): 104449, 2022 Mar.
Article in English | MEDLINE | ID: mdl-35144013

ABSTRACT

Inborn errors in copper metabolism result in a diverse set of abnormalities such as Wilson disease and MEDNIK syndrome. Homozygous pathogenic variants in AP1B1 lead to KIDAR (Keratitis-Ichthyosis-Deafness Syndrome). The main phenotypic features of KIDAR are ichthyosis, keratitis, erythroderma, and progressive hearing loss accompanied by developmental delay and failure to thrive. Herein, we describe a six-and-a-half-year-old boy with KIDAR caused by a novel pathogenic variant in AP1B1 (NM_001127.4:c.1263C > A, p.Tyr421*). The proband presented with ichthyosis, erythroderma, palmoplantar keratoderma, hearing loss, and corneal scarring. He also had hypotonia, global developmental delay, and photophobia. Lastly, we review all of the previously reported cases and the clinical features associated with KIDAR.


Subject(s)
Deafness , Ichthyosis , Keratitis , Adaptor Protein Complex 1/genetics , Adaptor Protein Complex beta Subunits/genetics , Child , Deafness/genetics , Humans , Ichthyosis/genetics , Ichthyosis/pathology , Keratitis/genetics , Keratitis/pathology , Male , Mutation
20.
Brain Dev ; 44(5): 329-335, 2022 May.
Article in English | MEDLINE | ID: mdl-35090779

ABSTRACT

BACKGROUND: Today, it is known that about 80 genes are involved in the etiology of hereditary spastic paraplegia. However, there are many cases whose etiology could not be determined by extensive genetic tests such as whole-exome sequencing, clinical exome. METHODS: Candidate genes were determined, since no clinically illuminating variant was detected in the whole-exome sequencing analysis of three patients, two of whom were siblings, with a complex hereditary spastic paraplegia phenotype. RESULTS: The p.Leu1202Pro variant in the SYNRG gene in the 1st and 2nd cases, and the p.Gly533* variant in the 3rd case were homozygous. DISCUSSION: We suggest that the SYNRG gene interacting with AP-1 (adaptor-related protein) from the AP complex family may cause the complex hereditary spastic paraplegia phenotype with extensive clinical spectrum. It may be important to evaluate SYNRG gene variants in patients with hereditary spastic paraplegia whose etiology has not been clarified.


Subject(s)
Adaptor Protein Complex 1/genetics , Spastic Paraplegia, Hereditary , Homozygote , Humans , Mutation/genetics , Pedigree , Phenotype , Spastic Paraplegia, Hereditary/genetics
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