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1.
J Leukoc Biol ; 106(3): 653-664, 2019 09.
Article in English | MEDLINE | ID: mdl-31329326

ABSTRACT

Blomia tropicalis mite is highly prevalent in tropical and subtropical regions and it is associated with allergic diseases such as rhinitis and asthma. By using an OVA-model of allergic lung disease, we have previously shown that sensitization in the presence of toll like receptors (TLRs) agonists attenuates subsequent OVA-induced allergic responses. Here, we evaluated the effect of CpG-ODN, a specific synthetic TLR-9 agonist, on the development of experimental asthma induced by Blomia tropicalis extract, a relevant source of aeroallergens. Among different protocols of Blomia tropicalis extract sensitization, the subcutaneous sensitization in the presence of alum adjuvant induced the highest Th2 responses, including high IgE levels. Adsorption of CpG to Blomia tropicalis extract/Alum attenuated the airway hyperreactivity, the infiltration of inflammatory cells including eosinophils, and the IL-5 content in BAL. In addition, lung peribronchial inflammatory infiltrate, mucus production and IL-5-producing CD3+ CD4+ T cells were significantly reduced in the Blomia tropicalis extract/Alum+CpG group. Importantly, CpG inhibited total IgE production as well as active systemic or cutaneous anaphylaxis reactions. Inhibition of pulmonary Th2 responses was associated with increased IL-10 production but not with IFN-γ production. Notably, in IL-10-deficient mice, sensitization with OVA/Alum+CpG resulted in intense lung neutrophilia and IFN-γ production, indicating that IL-10 is necessary to inhibit subsequent Th1 immunity. Our work highlights the mechanisms of allergy attenuation by CpG and it indicates the potential use of Alum-based formulation with CpG to treat allergic processes.


Subject(s)
Adjuvants, Immunologic/therapeutic use , Alum Compounds/chemistry , Asthma/prevention & control , Asthma/parasitology , Pyroglyphidae/physiology , Toll-Like Receptor 9/agonists , Adjuvants, Immunologic/pharmacology , Adsorption , Anaphylaxis/complications , Anaphylaxis/immunology , Anaphylaxis/parasitology , Animals , Asthma/complications , Cytokines/biosynthesis , Disease Models, Animal , Eosinophils/pathology , Female , Hypersensitivity/complications , Hypersensitivity/immunology , Hypersensitivity/parasitology , Immunity/drug effects , Immunization , Interleukin-10/metabolism , Interleukin-4/biosynthesis , Lymph Nodes/drug effects , Lymph Nodes/immunology , Lymph Nodes/pathology , Lymphocyte Subsets/drug effects , Lymphocyte Subsets/immunology , Mice, Inbred C57BL , Neutrophils/pathology , Oligodeoxyribonucleotides/pharmacology , Pyroglyphidae/drug effects , Th1 Cells/drug effects , Th1 Cells/immunology , Th2 Cells/drug effects , Th2 Cells/immunology , Toll-Like Receptor 9/metabolism
2.
Rev Alerg Mex ; 66(4): 499-503, 2019.
Article in Spanish | MEDLINE | ID: mdl-32105432

ABSTRACT

BACKGROUND: Oral anaphylaxis due to food intake contaminated with mites (OMA, oral mite anaphylaxis) is an allergic reaction that can represent life risk for patients. There are reports of cases from different parts of the world and they involve mainly processed foods based on wheat flour contaminated with mites of several families of the Astigmatina cohort. However, it remains as a little known and difficult to diagnose syndrome. The objective of this study was to describe the first clinical case of OMA due to the consumption of oat flakes in Panama City, Panama. CLINICAL CASE: Female patient of 39-years-old was entered an emergency room with allergic cutaneous and respiratory symptoms one hour after ingesting granola and oatmeal with milk at breakfast. After antiallergic treatment, the cause of allergy was determined by applying skin prick tests for ingested food and house mites. Results indicated a negative reaction for food and positive for mites. The mite analysis of the food samples directed in the identification of the species Blattisocius keegani and Suidasia pontifica, with a high density of the latter in flaked oats. CONCLUSION: This work is the first worldwide report of anaphylaxis due to consumption of oats contaminated with mites.


Antecedentes: La anafilaxia oral por ingesta de alimentos contaminados con ácaros (OMA) es una reacción alérgica que puede representar riesgo de vida para los pacientes. Aun cuando se tienen reportes de casos de diversas partes del mundo que involucran principalmente alimentos elaborados con harina de trigo contaminada con ácaros de varias familias de la cohorte Astigmatina, OMA permanece como un síndrome poco conocido y de difícil diagnóstico. El objetivo del estudio fue describir el primer caso de OMA por consumo de avena en hojuelas ocurrido en la ciudad de Panamá, Panamá. Caso clínico: Mujer de 39 años que ingresó a urgencias de un hospital con cuadro alérgico cutáneo y respiratorio una hora después de ingerir granola y avena con leche en el desayuno. Después del tratamiento antialérgico se procedió a determinar la causa de la alergia mediante aplicación de pruebas de punción cutánea para los alimentos ingeridos y ácaros domésticos. Los resultados indicaron reacción negativa para alimentos y positiva para ácaros. El análisis acarológico de las muestras de alimentos resultaron en la identificación de las especies Blattisocius keegani y Suidasia pontifica, con alta densidad de la última en la muestra de avena. Conclusiones: El presente reporte constituye el primero en el mundo de anafilaxia por consumo de avena contaminada con ácaros.


Subject(s)
Anaphylaxis/parasitology , Avena/parasitology , Mites , Adult , Animals , Female , Humans , Panama
3.
Braz J Med Biol Res ; 50(7): e6071, 2017 Jul 03.
Article in English | MEDLINE | ID: mdl-28678921

ABSTRACT

Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. granulosus cyst fluid (EgCF). Crude antigen for EgCF was prepared from E. granulosus-infected sheep liver. Antigens were separated and identified by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE), two-dimensional gel electrophoresis (2-DE), and immunoblotting. Results of 1D SDS-PAGE and immunoblotting showed that 40.5 kDa protein was the major antigen of sIgE, and 35.5 kDa protein was the major antigen of sIgG1 in EgCF. Results of 2-DE and immunoblotting showed that main antigens of sIgE in EgCF were four proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 40.5 kDa. Main antigens of sIgG1 in EgCF were five proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 35.5 kDa. The antigens identified for sIgE and sIgG1 can provide critical insights into cellular and molecular mechanisms underlying anaphylactic shock induced by CE.


Subject(s)
Anaphylaxis/parasitology , Antigens, Helminth/immunology , Echinococcosis/complications , Echinococcus granulosus/immunology , Immunoglobulin E/blood , Immunoglobulin G/blood , Adolescent , Adult , Anaphylaxis/immunology , Animals , Antigens, Helminth/blood , Case-Control Studies , Child , Echinococcosis/immunology , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoblotting , Male , Young Adult
4.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;50(7): e6071, 2017. tab, graf
Article in English | LILACS | ID: biblio-951705

ABSTRACT

Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. granulosus cyst fluid (EgCF). Crude antigen for EgCF was prepared from E. granulosus-infected sheep liver. Antigens were separated and identified by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE), two-dimensional gel electrophoresis (2-DE), and immunoblotting. Results of 1D SDS-PAGE and immunoblotting showed that 40.5 kDa protein was the major antigen of sIgE, and 35.5 kDa protein was the major antigen of sIgG1 in EgCF. Results of 2-DE and immunoblotting showed that main antigens of sIgE in EgCF were four proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 40.5 kDa. Main antigens of sIgG1 in EgCF were five proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 35.5 kDa. The antigens identified for sIgE and sIgG1 can provide critical insights into cellular and molecular mechanisms underlying anaphylactic shock induced by CE.


Subject(s)
Humans , Animals , Male , Female , Child , Adolescent , Adult , Young Adult , Immunoglobulin E/blood , Immunoglobulin G/blood , Echinococcus granulosus/immunology , Echinococcosis/complications , Anaphylaxis/parasitology , Antigens, Helminth/immunology , Enzyme-Linked Immunosorbent Assay , Immunoblotting , Case-Control Studies , Echinococcosis/immunology , Electrophoresis, Polyacrylamide Gel , Anaphylaxis/immunology , Antigens, Helminth/blood
5.
APMIS ; 106(7): 709-16, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9740510

ABSTRACT

Ws/Ws rats are deficient in both mucosal- and connective tissue-type mast cells. To study the role of mast cells in active anaphylaxis, changes in vascular permeability in the trachea upon intravenous antigen challenge with Evans blue dye were examined in Ws/Ws, heterogenic Ws/+, and normal +/ + rats sensitized with the nematode Nippostrongylus brasiliensis. Antigen challenge resulted in fatal anaphylactic shock in some +/+ and Ws/+ rats, but not in Ws/Ws rats. Marked dye leakage developed within 30 min in the trachea of +/+ and Ws/+ rats, while Ws/Ws rats showed no substantial increases in the levels of vascular permeability. Ex vivo stimulation of sensitized lung fragments from +/+ animals with specific antigen induced significant releases of histamine and leukotriene (LT) C4, while sensitized Ws/Ws rat-lung fragments did not. In Ws/Ws rats, levels of nematode-specific IgE, IgG1 and IgG2a antibodies as well as levels of lung eosinophilia were not significantly different from those in +/+ rats. These results show that mast cell-deficient Ws/Ws rats fail to develop active anaphylaxis, and this is mediated probably by the lack of mast cell-derived mediators required for initiation of the reaction.


Subject(s)
Anaphylaxis/immunology , Anaphylaxis/parasitology , Lung/immunology , Lung/parasitology , Mast Cells/immunology , Mast Cells/parasitology , Nippostrongylus/immunology , Animals , Antibodies, Helminth/biosynthesis , Antibodies, Helminth/blood , Antigens, Helminth/immunology , Capillary Permeability , Cell Count , Histamine Release , Immunization , Leukotriene C4/metabolism , Lung/metabolism , Lung/pathology , Mast Cells/pathology , Rats , Rats, Mutant Strains , Strongylida Infections/immunology , Strongylida Infections/metabolism , Strongylida Infections/pathology , Trachea/blood supply
6.
Rev. mex. anestesiol ; 21(2): 138-40, abr.-jun. 1998. ilus
Article in Spanish | LILACS | ID: lil-248378

ABSTRACT

La enfermedad hidatidica (Equinococosis) existe en todo el mundo: Su frecuencia en el ser humano depende de la incidencia de la enfermedad en huéspedes intermediarios. El quiste hidatidico más frecuente es el causado por el Echinococcus Granulosus, estos pueden alojarse en cualquier órgano o tejido pero la mayoría de las veces se aloja en el hígado y pulmones. El goteo del líquido del quiste produce sensibilización y origina eosinofilia; si el goteo es importante, ya sea espontáneo, traumático o por intervención quirúrgica, puede presentar una reacción anafilactica grave o hasta mortal. Presentamos un caso de reacción anafilactica en un paciente con quiste hidatidico el cual fue tratado quirúrgicamente. Se presenta la revisión de la literatura y se sugiere un manejo transoperatorio


Subject(s)
Humans , Female , Adult , Anaphylaxis/etiology , Anaphylaxis/parasitology , Echinococcosis, Hepatic/surgery , Echinococcosis, Hepatic/complications , Echinococcosis, Hepatic/diagnosis , Tomography
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