ABSTRACT
Aphanomyces euteiches is an oomycete pathogen that causes the pea root rot. We investigated the potential role of early belowground defense in pea (susceptible plant) and faba bean (tolerant plant) at three days after inoculation. Pea and faba bean were inoculated with A. euteiches zoospores. Root colonization was examined. Root exudates from pea and faba bean were harvested and their impact on A. euteiches development were assessed by using in vitro assays. A. euteiches root colonization and the influence of the oomycete inoculation on specialized metabolites patterns and arabinogalactan protein (AGP) concentration of root exudates were also determined. In faba bean root, A. euteiches colonization was very low as compared with that of pea. Whereas infected pea root exudates have a positive chemotaxis index (CI) on zoospores, faba bean exudate CI was negative suggesting a repellent effect. While furanoacetylenic compounds were only detected in faba bean exudates, AGP concentration was specifically increased in pea.This work showed that early in the course of infection, host susceptibility to A. euteiches is involved via a plant-species specific root exudation opening new perspectives in pea root rot disease management.
Subject(s)
Aphanomyces/drug effects , Aphanomyces/growth & development , Pisum sativum/microbiology , Plant Exudates/pharmacology , Plant Roots/microbiology , Vicia faba/chemistry , Vicia faba/microbiology , Virulence/drug effects , Crops, Agricultural/microbiology , Host-Pathogen Interactions/drug effects , Plant Immunity/drug effectsABSTRACT
Crayfish plague, a devastating disease of freshwater crayfish, is caused by an oomycete organism, Aphanomyces astaci. Currently five genotypes of A. astaci are known, but variable features between the strains or genotypes have not been studied extensively. This study analysed 28 isolates of the As genotype and 25 isolates of the Ps1 genotype and reveals that the radial growth rate is significantly (P < 0.001) different between these two genotypes, although highly variable inside the genotype As. Two Ps1 genotype isolates and two As genotype isolates with different radial growth rates were tested in an infection trial. Clear differences were detected in the development of mortality in the test groups. The representatives of the Ps1 genotype caused total mortality within a short time span. The As genotype isolates were much less virulent. The slow-growing As isolate showed higher virulence than the As isolate with a high growth capacity. Although slow growth could be one survival strategy of the pathogen, several other mechanisms are involved in the pathogenicity and warrant further studies.
Subject(s)
Aphanomyces/physiology , Astacoidea/microbiology , Host-Pathogen Interactions , Animals , Aphanomyces/genetics , Aphanomyces/growth & development , Aphanomyces/pathogenicity , GenotypeABSTRACT
Isothiocyanates (ITCs) hydrolyzed from glucosinolates (GSLs) in Brassicaceae tissue are toxic to soil organisms. In this study, the effect of aliphatic and aromatic ITCs from hydrated dry Brassicaceae shoot tissues on the mycelium and oospores of the pea root rot pathogen Aphanomyces euteiches was investigated. The profile and concentrations of GSLs in two test Brassicaceae species, Sinapis alba and Brassica juncea, and the ITCs from the dominant hydrolyzed parent GSLs were monitored. The concentrations of dominant ITCs and pathogen exposure time were evaluated in in vitro experiments. The greatest effect on the pathogen was observed from aliphatic ITCs hydrolyzed from B. juncea tissue, and the effect depended on the ITC concentration and exposure time. ITCs were more effectively hydrolyzed from B. juncea GSLs than from S. alba GSLs; i.e., the ITC/GSL ratio was higher in B. juncea than in S. alba tissue, giving a different release pattern. The release of phenylethyl isothiocyanate, which was common to both species, followed a pattern similar to that of the dominant ITC in each crop species. This suggests that traits other than GSL content, e.g., plant cell structure, may affect the release of ITCs and should therefore influence the choice of species used for biofumigation purposes.
Subject(s)
Aphanomyces/drug effects , Brassicaceae/chemistry , Fungicides, Industrial/pharmacology , Isothiocyanates/pharmacology , Pisum sativum/microbiology , Plant Diseases/microbiology , Plant Extracts/pharmacology , Plant Shoots/chemistry , Aphanomyces/growth & development , Brassicaceae/metabolism , Dose-Response Relationship, Drug , Fungicides, Industrial/analysis , Fungicides, Industrial/metabolism , Isothiocyanates/analysis , Isothiocyanates/metabolism , Mycelium/drug effects , Mycelium/growth & development , Plant Extracts/analysis , Plant Extracts/metabolism , Plant Shoots/metabolismABSTRACT
Two polyketides, cryptosporiopsin A (1) and hydroxypropan-2',3'-diol orsellinate (3), and a natural cyclic pentapeptide (4), together with two known compounds were isolated from the culture of Cryptosporiopsis sp., an endophytic fungus from leaves and branches of Zanthoxylum leprieurii (Rutaceae). The structures of these metabolites were elucidated on the basis of their spectroscopic and spectrometric data. Cryptosporiopsin A and the other metabolites exhibited motility inhibitory and lytic activities against zoospores of the grapevine downy mildew pathogen Plasmopara viticola at 10-25µg/mL. In addition, the isolated compounds displayed potent inhibitory activity against mycelial growth of two other peronosporomycete phytopathogens, Pythium ultimum, Aphanomyces cochlioides and a basidiomycetous fungus Rhizoctonia solani. Weak cytotoxic activity on brine shrimp larvae was observed.
Subject(s)
Aphanomyces/drug effects , Artemia/drug effects , Ascomycota/chemistry , Peptides, Cyclic/pharmacology , Polyketides/pharmacology , Pythium/drug effects , Zanthoxylum/chemistry , Animals , Aphanomyces/growth & development , Ascomycota/metabolism , Molecular Conformation , Peptides, Cyclic/chemistry , Peptides, Cyclic/metabolism , Plant Leaves/chemistry , Plant Leaves/metabolism , Polyketides/chemistry , Polyketides/metabolism , Pythium/growth & development , Structure-Activity Relationship , Zanthoxylum/metabolismABSTRACT
Root tips of many plant species release a number of border, or border-like, cells that are thought to play a major role in the protection of root meristem. However, little is currently known on the structure and function of the cell wall components of such root cells. Here, we investigate the sugar composition of the cell wall of the root cap in two species: pea (Pisum sativum), which makes border cells, and Brassica napus, which makes border-like cells. We find that the cell walls are highly enriched in arabinose and galactose, two major residues of arabinogalactan proteins. We confirm the presence of arabinogalactan protein epitopes on root cap cell walls using immunofluorescence microscopy. We then focused on these proteoglycans by analyzing their carbohydrate moieties, linkages, and electrophoretic characteristics. The data reveal (1) significant structural differences between B. napus and pea root cap arabinogalactan proteins and (2) a cross-link between these proteoglycans and pectic polysaccharides. Finally, we assessed the impact of root cap arabinogalactan proteins on the behavior of zoospores of Aphanomyces euteiches, an oomycetous pathogen of pea roots. We find that although the arabinogalactan proteins of both species induce encystment and prevent germination, the effects of both species are similar. However, the arabinogalactan protein fraction from pea attracts zoospores far more effectively than that from B. napus. This suggests that root arabinogalactan proteins are involved in the control of early infection of roots and highlights a novel role for these proteoglycans in root-microbe interactions.
Subject(s)
Aphanomyces/cytology , Aphanomyces/growth & development , Brassica napus/metabolism , Chemotaxis/drug effects , Mucoproteins/pharmacology , Pisum sativum/metabolism , Plant Root Cap/metabolism , Aphanomyces/drug effects , Brassica napus/cytology , Brassica napus/drug effects , Brassica napus/microbiology , Cell Wall/drug effects , Cell Wall/metabolism , Chemical Precipitation , Electrophoresis, Polyacrylamide Gel , Glucosides/metabolism , Microscopy, Fluorescence , Monosaccharides/chemistry , Monosaccharides/metabolism , Mucoproteins/chemistry , Pisum sativum/cytology , Pisum sativum/drug effects , Pisum sativum/microbiology , Phloroglucinol/analogs & derivatives , Phloroglucinol/metabolism , Plant Proteins/chemistry , Plant Proteins/pharmacology , Plant Root Cap/cytology , Plant Root Cap/drug effectsABSTRACT
Epizootic ulcerative syndrome (EUS) is a disease affecting both wild and farmed fish in freshwater and estuarine environments. After it was first described in Japan in 1971, the disease has spread widely across Asia and to some regions of Australia, North America and Africa. In Asia and Africa, the spread of the disease has substantially affected livelihoods of fish farmers and fishermen. No reports are yet published showing the presence of the disease in Europe or South America. Given its epizootic nature and its broad susceptible fish species range, it would appear that the disease has the potential for further spread. This study provides a review of the scientific literature on several biological factors of the pathogen, Aphanomyces invadans, associated with the disease EUS and aspects of the disease that are relevant to undertaking import risk assessments (IRA) covering (i) Life cycle and routes of transmission; (ii) Minimum infectious dose; (iii) Tissue localization and pathogen load; (iv) Predisposing factors for infection and factors influencing expression of disease; (v) Carrier state in fish; (vi) Diagnostic methods; (vii) Survival in the environment; (viii) Permissive temperature range; (ix) Stability of the agent in aquatic animal products; (x) Prevalence of infection; and (xi) Affected life stages. Much of the biological information presented is relevant to a broad range of risk questions. Areas where data are lacking were identified, and the information provided is put into context with other aspects that need to be addressed in an IRA.
Subject(s)
Aphanomyces/pathogenicity , Fish Diseases , Infections/veterinary , Animals , Aphanomyces/growth & development , Fish Diseases/diagnosis , Fish Diseases/epidemiology , Fish Diseases/microbiology , Fish Diseases/transmission , Fishes , Infections/diagnosis , Infections/epidemiology , Infections/microbiology , Infections/transmission , Risk AssessmentSubject(s)
Aphanomyces/growth & development , Astacoidea/microbiology , Infections/veterinary , Animals , Aphanomyces/genetics , Conservation of Natural Resources , DNA, Fungal/chemistry , DNA, Fungal/genetics , Hungary , Infections/microbiology , Introduced Species , Polymerase Chain Reaction , RiversABSTRACT
Sterols are isoprenoid-derived molecules that have essential functions in eukaryotes but whose metabolism remains largely unknown in a large number of organisms. Oomycetes are fungus-like microorganisms that are evolutionarily related to stramenopile algae, a large group of organisms for which no sterol metabolic pathway has been reported. Here, we present data that support a model of sterol biosynthesis in Aphanomyces euteiches, an oomycete species causing devastating diseases in legume crops. In silico analyses were performed to identify genes encoding enzymes involved in the conversion of the isoprenoid precursor 3-hydroxy-3-methylglutaryl coenzyme A to isoprenoids. Several metabolic intermediates and two major sterol end-products were identified by gas chromatography-mass spectroscopy. We show that A. euteiches is able to produce fucosterol (a sterol initially identified in brown algae) and cholesterol (the major animal sterol). Mycelium development is inhibited by two sterol demethylase inhibitors used as fungicides, namely tebuconazole and epoxiconazole. We propose the first sterol biosynthetic pathway identified in a stramenopile species. Phylogenetic analyses revealed close relationships between A. euteiches enzyme sequences and those found in stramenopile algae, suggesting that part of this pathway could be conserved in the Stramenopila kingdom.
Subject(s)
Aphanomyces/metabolism , Fabaceae/microbiology , Plant Roots/microbiology , Sterols/metabolism , Aphanomyces/genetics , Aphanomyces/growth & development , Cholesterol/metabolism , Fabaceae/metabolism , Gas Chromatography-Mass Spectrometry , Lanosterol/metabolism , Phylogeny , Plant Roots/metabolism , Sterols/biosynthesis , Stigmasterol/analogs & derivatives , Stigmasterol/metabolismABSTRACT
A comprehensive proteomic approach was applied to investigate molecular events occurring upon inoculation of Medicago truncatula cell-suspension cultures with the oomycete root pathogen Aphanomyces euteiches. Establishment of an inoculation assay in the cell cultures allowed a direct comparison between proteins induced by elicitation with a crude culture extract of the oomycete and by inoculation with A. euteiches zoospores representing the natural infection carrier. Oxidative burst assays revealed responsiveness of the cell cultures for perception of elicitation and inoculation signals. The plant "elicitation proteome" resembles the "inoculation proteome" in early incubation stages and includes proteins induced following initial oxidative burst and defense reactions, but also proteins involved in the antioxidative system. However, approximately 2 days after incubation, the inoculation proteome differs drastically from the proteome of elicited cultures, where a cessation of responses assignable to A. euteiches elicitation occurred. The specific protein induction patterns of zoospore-inoculated cells appeared consistent with the protein induction identified in recent studies for an A. euteiches infection in planta and consist of three functional groups: i) pathogenesis-related proteins, ii) proteins associated with secondary phenylpropanoid or phytoalexin metabolism, and, particularly, iii) proteins assigned to carbohydrate metabolism and energy-related cellular processes. Phosphoproteomic analyses revealed consistent and specific activation of these defense-related pathways already at very early timepoints of inoculation, providing evidence that the identified protein profiles are representative for an established A. euteiches infection of M. truncatula.
Subject(s)
Aphanomyces/growth & development , Medicago truncatula/metabolism , Plant Diseases/genetics , Plant Proteins/metabolism , Cells, Cultured , Electrophoresis, Gel, Two-Dimensional , Gene Expression Regulation, Plant , Hydrogen Peroxide/metabolism , Medicago truncatula/genetics , Medicago truncatula/microbiology , Phosphorylation , Plant Diseases/microbiology , Plant Proteins/genetics , Plant Roots/genetics , Plant Roots/metabolism , Plant Roots/microbiology , Proteome/metabolism , ProteomicsABSTRACT
Molecular phylogenetic relationships among 12 species of Aphanomyces de Bary (Oomycetes) were analyzed based on 108 ITS sequences of nuclear rDNA. Sequences used in the analyses belonged to the major species currently available in pure culture and GenBank. Bayesian, maximum likelihood, and maximum parsimony analyses support that Aphanomyces constitutes a monophyletic group. Three independent lineages were found: (i) plant parasitic, (ii) animal parasitic, and (iii) saprotrophic or opportunistic parasitic. Sexual reproduction appeared to be critical in plant parasites for survival in soil environments while asexual reproduction seemed to be advantageous for exploiting specialization in animal parasitism. Repeated zoospore emergence seems to be an advantageous property for both plant and animal parasitic modes of life. Growth in unspecific media was generally faster in saprotrophs compared with parasitic species. A number of strains and GenBank sequences were found to be misidentified. It was confirmed molecularly that Aphanomyces piscicida and Aphanomyces invadans appear to be conspecific, and found that Aphanomyces iridis and Aphanomyces euteiches are closely related, if not the same, species. This study has shown a clear evolutionary separation between Aphanomyces species that are plant parasites and those that parasitize animals. Saprotrophic or opportunistic species formed a separate evolutionary lineage except Aphanomyces stellatus whose evolutionary position has not yet been resolved.
Subject(s)
Aphanomyces/classification , Aphanomyces/isolation & purification , Animals , Aphanomyces/genetics , Aphanomyces/growth & development , Cluster Analysis , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Molecular Sequence Data , Parasitic Diseases, Animal/microbiology , Phylogeny , Plants/parasitology , Sequence Analysis, DNA , Soil Microbiology , Spores, Fungal/growth & developmentABSTRACT
Aphanomyces euteiches is an oomycete pathogen that causes seedling blight and root rot of legumes, such as alfalfa and pea. The genus Aphanomyces is phylogenically distinct from well-studied oomycetes such as Phytophthora sp., and contains species pathogenic on plants and aquatic animals. To provide the first foray into gene diversity of A. euteiches, two cDNA libraries were constructed using mRNA extracted from mycelium grown in an artificial liquid medium or in contact to plant roots. A unigene set of 7,977 sequences was obtained from 18,864 high-quality expressed sequenced tags (ESTs) and characterized for potential functions. Comparisons with oomycete proteomes revealed major differences between the gene content of A. euteiches and those of Phytophthora species, leading to the identification of biosynthetic pathways absent in Phytophthora, of new putative pathogenicity genes and of expansion of gene families encoding extracellular proteins, notably different classes of proteases. Among the genes specific of A. euteiches are members of a new family of extracellular proteins putatively involved in adhesion, containing up to four protein domains similar to fungal cellulose binding domains. Comparison of A. euteiches sequences with proteomes of fully sequenced eukaryotic pathogens, including fungi, apicomplexa and trypanosomatids, allowed the identification of A. euteiches genes with close orthologs in these microorganisms but absent in other oomycetes sequenced so far, notably transporters and non-ribosomal peptide synthetases, and suggests the presence of a defense mechanism against oxidative stress which was initially characterized in the pathogenic trypanosomatids.
Subject(s)
Aphanomyces/genetics , Gene Expression Profiling , Genes, Fungal , Metabolic Networks and Pathways , Oomycetes/metabolism , Proteome/analysis , Virulence Factors/metabolism , Aphanomyces/growth & development , Aphanomyces/metabolism , Expressed Sequence Tags , Gene Library , Membrane Proteins/metabolism , Phytophthora/metabolism , Plant Roots/growth & development , Plant Roots/metabolismABSTRACT
The aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. For this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in Europe. The analysis of microbial communities by Single Strand Conformation Polymorphism (SSCP) of 16S/18S rRNA clearly revealed the existence of discrete microenvironment- and site-specific patterns. A total of 1952 bacterial and 1344 fungal isolates screened by dual testing for antagonism toward the pathogens Aphanomyces cochlioides, Phoma betae, Pythium ultimum, and Rhizoctonia solani resulted in 885 bacterial (=45%) and 437 fungal (=33%) antagonists. In general, the indigenous antagonistic potential was very high and influenced by (a) the location, (b) the plant developmental stage, and (3) the microenvironment. Furthermore, we showed for the first time that the antagonistic potential was highly specific for each target pathogen. The majority of antagonistic microorganisms suppressed only one pathogen (bacteria: 664 = 75%; fungi: 256 = 59%), whereas the minority showed a broad host range (bacteria: 4 = 0.5%; fungi: 7 = 1.6%). The bacterial communities harbored the highest antagonistic potential against P. ultimum, whereas the fungal communities contained more antagonists against A. cochlioides and R. solani. In contrast to their high proportion, only a low diversity of antagonists at genotypic and species level was found. Novel antagonistic species, e.g., Subtercola pratensis or Microbacterium testaceum were found in the internal part of the sugar beet body.
Subject(s)
Antibiosis , Bacteria , Beta vulgaris/microbiology , Ecosystem , Fungi , Plant Diseases/microbiology , Aphanomyces/growth & development , Aphanomyces/pathogenicity , Ascomycota/growth & development , Ascomycota/pathogenicity , Bacteria/classification , Bacteria/genetics , Bacteria/growth & development , Bacteria/isolation & purification , Fungi/classification , Fungi/genetics , Fungi/growth & development , Fungi/isolation & purification , Molecular Sequence Data , Plant Leaves/microbiology , Plant Roots/microbiology , Polymorphism, Single-Stranded Conformational , Pythium/growth & development , Pythium/pathogenicity , RNA, Ribosomal, 16S/genetics , Rhizoctonia/growth & development , Rhizoctonia/pathogenicity , Sequence Analysis, DNAABSTRACT
Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was efficiently knocked down in two MtPr10-1i lines. Moreover, five additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as compared to that in roots of the transformation controls. In addition, MtPr10-1 silencing led to the induction of a new set of PR proteins after infection with A. euteiches including the de novo induction of two isoforms of thaumatin-like proteins (PR-5b), which were not detectable in A. euteiches-infected control roots. Thus, antagonistic induction of other PR proteins, which are normally repressed due to PR-10 expression, is supposed to cause an increased resistance of M. truncatula upon an A. euteiches in vitro infection. The results were also further confirmed by detecting increased PR-5b induction levels in 2-D gels of a previously analyzed M. truncatula line (F83.005-9) exhibiting increased A. euteiches tolerance associated with reduced PR-10 induction levels.
Subject(s)
Aphanomyces/growth & development , Medicago truncatula/microbiology , RNA Interference , Electrophoresis, Gel, Two-Dimensional , Mass Spectrometry , Medicago truncatula/genetics , Phenotype , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Roots/chemistry , Plants, Genetically Modified , Root Nodules, Plant/microbiologyABSTRACT
To investigate the molecular mechanisms underlying susceptibility of legumes to the root pathogen Aphanomyces euteiches (oomycota), comparative proteomic studies have been carried out. In a first approach, we have analysed two Medicago truncatula lines of the French CORE collection (F83.005-5 (R2002) and F83.005-9 (R2002)), which showed either increased or decreased susceptibility to A. euteiches as compared to the widely adopted line A17. Several proteins were identified to be differentially induced after pathogen challenge in the two M. truncatula accessions with altered disease susceptibility, whereof proteins with increased abundances in the more resistant line F83.005-9 could be involved in mechanisms that lead to an improved disease resistance. Among these proteins, we identified two proteasome alpha subunits, which might be involved in defense response. To broaden our studies on A. euteiches-tolerance of M. truncatula, we investigated two other phenomena that lead to an either increased A. euteiches-resistance or to an enhanced susceptibility. The topic of an enhanced plant resistance to A. euteiches was studied in plants showing a bioprotective effect of a pre-established arbuscular mycorrhiza (AM) symbiosis. Evaluation of root fresh weights and pathogen spreading in the root system clearly indicate that mycorrhizal plants show increased A. euteiches-resistance as compared to non-mycorrhizal plants. Proteome analyses revealed the induction of similar protein patterns as in the M. truncatula accessions with comparatively high resistance level to A. euteiches. In a third approach, increased A. euteiches susceptibility was effected by exogenous abscisic acid (ABA) application prior to root infection. Evaluation of the abundance levels of a group of pathogenesis related class 10 (PR10)-like proteins, which were previously identified to be regulated after A. euteiches infection, revealed a correlation between the abundance levels of these proteins and the A. euteiches infection level or severity.
Subject(s)
Aphanomyces/growth & development , Medicago truncatula/metabolism , Plant Proteins/analysis , Proteome/analysis , Abscisic Acid/pharmacology , Electrophoresis, Gel, Two-Dimensional , Immunity, Innate , Mass Spectrometry/methods , Medicago truncatula/drug effects , Medicago truncatula/microbiology , Mycorrhizae/growth & development , Plant Diseases/microbiology , Plant Growth Regulators/pharmacology , Plant Roots/drug effects , Plant Roots/metabolism , Plant Roots/microbiology , Species SpecificityABSTRACT
The legume root rot disease caused by the oomycete pathogen Aphanomyces euteiches is one major yield reducing factor in legume crop production. A comparative proteomic approach was carried out in order to identify proteins of the model legume Medicago truncatula which are regulated after an infection with A. euteiches . Several proteins were identified by two dimensional gel electrophoresis to be differentially expressed after pathogen challenge. Densitometric evaluation of expression values showed different regulation during the time-course analysed. Proteins regulated during the infection were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Among the differentially expressed proteins, two encoded putative cell wall proteins and two were designated as small heat shock proteins. Furthermore, an isoform of the chalcone-O-methyltransferase was found to be increased in infected roots. The majority of induced proteins belonged to the family of class 10 of pathogenesis related proteins (PR10). Previously, various PR10-like proteins have been shown to be regulated by general stress or abscisic acid (ABA). Therefore, these proteins were further investigated concerning their regulation in response to drought stress and exogenous ABA-application. Complex regulation patterns were identified: three of the A. euteiches -induced PR10-like proteins were also induced by exogenous ABA- but none of them is induced after drought stress. In contrast, three of these proteins are down-regulated by drought stress. Hence, the strong expression of different PR10-family members and their regulation profiles indicates that this set of proteins plays a major role during root adaptations to various stress conditions.
Subject(s)
Aphanomyces/growth & development , Medicago truncatula/metabolism , Plant Proteins/analysis , Plant Roots/metabolism , Proteome/analysis , Abscisic Acid/pharmacology , Amino Acid Sequence , Down-Regulation/drug effects , Electrophoresis, Gel, Two-Dimensional , Medicago truncatula/genetics , Medicago truncatula/microbiology , Molecular Sequence Data , Plant Proteins/genetics , Plant Roots/genetics , Plant Roots/microbiology , Proteome/genetics , Proteomics/methods , Sequence Homology, Amino Acid , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
A diagnostic procedure, based on a polymerase chain reaction method (PCR) was developed to detect infection of crayfish with the Oomycete Aphanomyces astaci. A set of oligonucleotide primers was designed to specifically amplify A. astaci DNA in the ITS region surrounding the 5.8S rDNA gene. The PCR amplifies a 115bp amplicon. The specificity of the primers was demonstrated by testing on 27 A. astaci strains and against 20 non-A. astaci Oomycetes and 5 fungal species. Most of the non-A. astaci Oomycete or fungal species included in the study are either known parasites of freshwater crayfish cuticle or can be found in their natural environment. Specificity was also tested against crayfish tissue and some known parasites and bacteria infecting crayfish. A protocol for the extraction of A. astaci DNA from infected crayfish tissue was developed. The optimised method allows the detection of two genome equivalents of purified A. astaci genomic DNA. The method was tested on noble crayfish (Astacus astacus), artificially infected with A. astaci. Detection of A. astaci was possible at the very first time of sampling, which was 2 days after the beginning of spore exposure.
Subject(s)
Aphanomyces/genetics , Astacoidea/microbiology , Animals , Aphanomyces/growth & development , Base Sequence , DNA/chemistry , DNA/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Male , Molecular Sequence Data , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/veterinary , RNA, Ribosomal, 5.8S/chemistry , RNA, Ribosomal, 5.8S/genetics , Sensitivity and Specificity , Sequence AlignmentABSTRACT
Aphanomyces invadans (Saprolegniaceae) is a peronosporomycete fungus associated with the serious fish disease, epizootic ulcerative syndrome (EUS), also known as mycotic granulomatosis. In this study, interspecific relationships were examined between A. invadans isolates and other aquatic animal pathogenic Saprolegniaceae, and saprophytic Saprolegniaceae from EUS-affected areas. Restriction fragment length polymorphisms and sequences of ribosomal DNA confirmed that A. invadans is distinct from all other species studied. A sequence from the internal transcribed spacer region ITS1, unique to A. invadans, was used to design primers for a PCR-based diagnostic test. Intraspecific relationships were also examined by random amplification of polymorphic DNA using 20 isolates of A. invadans from six countries. The isolates showed a high degree of genetic homogeneity using 14 random ten-mer primers. This provides evidence that the fungus has spread across Asia in one relatively rapid episode, which is consistent with reports of outbreaks of EUS. Physiological distinctions between A. invadans and other Aphanomyces species based on a data set of 16 growth parameters showed remarkable taxonomic congruence with the molecular phylogeny.