Phytochemical Screening and chemical investigation of lipoidal matter of the leaves of Latania verschaffeltii Lem. Family Arecaceae cultivated in Egypt

Abstract This study presents the first preliminary phytochemical screening and investigation of the lipoidal matter of Latania verschaffeltii Lem. leaves, belonging to the Arecaceae family. Gas chromatography coupled with mass spectroscopy (GC/MS) was used to analyze and identify compounds of saponifiable and unsaponifiable content. The preliminary phytochemical screening of total methanolic extract of Latania verschaffeltii Lem. leaves revealed the presence of unsaturated sterols and/or triterpenes, carbohydrates and/or glycosides, flavonoids, tannins, saponins, and phenolic acids in the leaves. However, cardenolides, cyanogenic compounds, alkaloids, and iridoids were not detected. The results of the gas chromatography/mass spectrometry (GC/MS) analysis indicated that the percentage of saturated fatty acids (83.82%) is higher than that of unsaturated fatty acids (9.42%). The predominant methyl ester of a saturated fatty acid detected in the sample was hexadecanoic acid methyl ester, accounting for 41.68% of the total. The composition of the unsaponifiable matter consisted of hydrocarbons (5.66%), fatty alcohols (0.96%), terpenes (85.97%), and sterols (2.18%). The major terpenes observed were phytol (43.62%) and squalene (39.27%).

Levantamento das espécies frutíferas em áreas de agricultura familiar no município de Santarém, Pará / Fruit species cultivated in family farms in the municipality of Santarém, Pará

As espécies frutíferas se apresentam como estratégia importante para garantia da segurança e soberania alimentar de populações tradicionais. Diante disso, o objetivo do trabalho foi estimar a riqueza e registrar as espécies frutíferas cultivadas em quintais de agricultura familiar no município de Santarém, Pará. O levantamento das espécies frutíferas foi realizado em 13 quintais das famílias visitadas, com registros de 520 indivíduos pertencentes a 39 espécies, 29gêneros e 16 famílias botânicas. Dentre os indivíduos avaliados, destacaram-se as famílias Arecaceae (6 spp.) e Myrtaceae (5 spp.). Em média, foram encontradas 14 espécies frutíferas por quintal, evidenciando que o cultivo dessas espécies está fortemente associado ao suprimento dademanda por alimentos para o consumo familiar. Das espécies frutíferas, as mais frequentes foram a Persea americana e Cocos nucifera com 100% de representatividade, seguidas pela Theobroma grandiflorum com 92%. A riqueza de espécies frutíferas encontradosnos quintais das famílias visitadas revela a importância desses ambientes na soberania e segurança alimentar e nutricional. Além disso, os quintais destacam-se pelo seu papel estratégico na introdução de árvores nas paisagens agrícolas. Diante disso, recomenda-se a execução de trabalhos futuros com foco no papel dos quintais na geração de alimentos de qualidade, visando subsidiar o fomento de políticas públicas com incentivo à produção de alimentos nesses ambientes.(AU)

Fruit species are an important strategy to guarantee food security and sovereignty for traditional populations. Therefore, the objective of this work was to estimate the richness and record the fruit species cultivated in family farms in the municipality of Santarém, Pará. The survey of fruit species was carried out in 13 homegardens of the families visited, with records of 520 individuals belonging to 39 species, 29 genera and 16 botanical families. Among the evaluated individuals, the families Arecaceae (6 spp.) and Myrtaceae (5 spp.) stood out. On average, 14 fruit species were found per yard, showing that the cultivation of these species is strongly associated with supplying the demand for food for family consumption. Of the fruit species, the most frequent were Persea americanaand Cocos nuciferawith 100% representation, followed by Theobroma grandiflorumwith 92%. The richness of fruit species found in the homegardens of the families visited revealsthe importance of these environments in food and nutrition sovereignty and security. In addition, homegardens stand out for their strategic role in introducing trees into agricultural landscapes. In view of this, it is recommended to carry out future workfocusing on the role of homegardens in the generation of quality food, aiming to subsidize the promotion of public policies to encourage the production of food in these environments.(AU)

Development and characterization of photoprotective nanoemulsions containing Babassu (Orbignya phalerata Mart. ) lipophilic extract

Abstract Oil-in-water photoprotective nanoemulsions (NEs) were developed using Babassu (BBS) lipophilic extract, nonionic surfactants, and low concentrations of organic sunscreens by ultrasonic processing. BBS extract was chosen due to its suitable physicochemical properties (acidity index, peroxide index, refraction index, and relative density) and predominance of saturated fatty acids, identified by gas chromatography-mass spectrometry (GC-MS), which promote biological activities and high oxidative stability. NEs were characterized by mean droplet size, morphology, polydispersity index (PdI), pH, and organoleptic properties, and the physical stability of the NEs was evaluated for 120 days at room temperature. The sun protection factor (SPF) was determined, and the photostability and in vitro cytotoxicity assays were performed for NEs. All NEs remained stable for 120 days, with a droplet size <150 nm and a monomodal distribution profile. The pH values were compatible with the skin's pH. NE3 showed a spherical morphology, with a mean droplet size of 125.15 ± 0.16 nm and PdI of 0.145 ± 0.032. NE3 containing BBS extract and sunscreens presented an SPF of 35.5 ± 3.0, was photostable after 6 h of radiation and was non-cytotoxic to fibroblast cells. Thus, NE3 could be considered a promising formulation for developing synergic plant-extract sunscreen photoprotective products for the market

Diversity and genetic structure of natural populations of the palm tree Euterpe precatoria (Arecaceae) / Diversidad y estructura genética de poblaciones naturales de la palmera Euterpe precatoria (Arecaceae)

Abstract Introduction: The natural ecosystems of northern Mato Grosso, Brazil, are in process of fragmentation, mainly due to population growth and the expansion of agriculture. This endangers the palm Euterpe precatoria (locally known as açaí), used for construction, palm hearts, juices and ice cream. Objective: To evaluate the local diversity and genetic structure in native populations of E. precatoria. Methods: We collected leaves from 106 fruiting palms from five populations in Mato Grosso State, for analysis of microsatellite markers with Polymerase Chain Reaction. Results: The five SSR loci revealed a total of 30 alleles, ranging from 5 (EE23 and EE43) to 7 (EE2 and EE15), with an average of 6 alleles per locus. The mean PIC was 0.74 and confirmed low heterozygosity and inbreeding. The UPGMA dendrogram produced two groups and molecular variance revealed greater genetic differentiation within populations. The high levels of homozygous microsatellite loci indicate low genetic diversity. Conclusions: These populations have low gene diversity, high average number of alleles per locus, and rare and exclusive alleles. We recommend the establishment of permanent conservation units with corridors among them.

Resumen Introducción: Los ecosistemas naturales del norte de Mato Grosso, Brasil, están en proceso de fragmentación, principalmente debido al crecimiento de la población y la expansión de la agricultura. Esto pone en peligro la palma Euterpe precatoria (localmente conocida como açaí), utilizada para la construcción, extracción de palmito, preparación de jugos y helados. Objetivo: Evaluar la diversidad local y estructura genética en poblaciones nativas de E. precatoria. Métodos: Recolectamos hojas de 106 palmas fructíferas de cinco poblaciones en el estado de Mato Grosso, para análisis de marcadores microsatélites con el método de Reacción en Cadena de la Polimerasa (PCR). Resultados: Los cinco loci SSR revelaron un total de 30 alelos, que van desde 5 (EE23 y EE43) hasta 7 (EE2 y EE15), con un promedio de 6 alelos por locus. El PIC medio fue de 0.74 y confirmó baja heterocigosidad y endogamia en las poblaciones. El dendrograma UPGMA produjo dos grupos y la varianza molecular reveló una mayor diferenciación genética dentro de las poblaciones. Los loci de microsatélites presentaron un alto nivel de homocigotos lo que indica una baja diversidad genética. Conclusiones: Estas poblaciones tienen baja diversidad genética, alto promedio de alelos por locus y alelos raros y únicos. Recomendamos el establecimiento de unidades de conservación permanentes con corredores entre ellas.

The plastome sequence of Bactris gasipaes and evolutionary analysis in tribe Cocoseae (Arecaceae).

The family Arecaceae is distributed throughout tropical and subtropical regions of the world. Among the five subfamilies, Arecoideae is the most species-rich and still contains some ambiguous inter-generic relationships, such as those within subtribes Attaleinae and Bactridineae. The hypervariable regions of plastid genomes (plastomes) are interesting tools to clarify unresolved phylogenetic relationships. We sequenced and characterized the plastome of Bactris gasipaes (Bactridinae) and compared it with eight species from the three Cocoseae sub-tribes (Attaleinae, Bactridinae, and Elaeidinae) to perform comparative analysis and to identify hypervariable regions. The Bactris gasipaes plastome has 156,646 bp, with 113 unique genes. Among them, four genes have an alternative start codon (cemA, rps19, rpl2, and ndhD). Plastomes are highly conserved within tribe Cocoseae: 97.3% identity, length variation of ~2 kb, and a single ~4.5 kb inversion in Astrocaryum plastomes. The LSC/IR and IR/SSC junctions vary among the subtribes: in Bactridinae and Elaeidinae the rps19 gene is completely contained in the IR region; in the subtribe Attaleinae the rps19 gene is only partially contained in the IRs. The hypervariable regions selected according to sequence variation (SV%) and frequency of parsimony informative sites (PIS%) revealed plastome regions with great potential for molecular analysis. The ten regions with greatest SV% showed higher variation than the plastid molecular markers commonly used for phylogenetic analysis in palms. The phylogenetic trees based on the plastomes and the hypervariable regions (SV%) datasets had well-resolved relationships, with consistent topologies within tribe Cocoseae, and confirm the monophyly of the subtribes Bactridinae and Attaleinae.

Plastid genome evolution in Amazonian açaí palm (Euterpe oleracea Mart.) and Atlantic forest açaí palm (Euterpe edulis Mart.).

KEY MESSAGE: The plastomes of E. edulis and E. oleracea revealed several molecular markers useful for genetic studies in natural populations and indicate specific evolutionary features determined by vicariant speciation. Arecaceae is a large and diverse family occurring in tropical and subtropical ecosystems worldwide. E. oleracea is a hyperdominant species of the Amazon forest, while E. edulis is a keystone species of the Atlantic forest. It has reported that E. edulis arose from vicariant speciation after the emergence of the belt barrier of dry environment (Cerrado and Caatinga biomes) between Amazon and Atlantic forests, isolating the E. edulis in the Atlantic forest. We sequenced the complete plastomes of E. edulis and E. oleracea and compared them concerning plastome structure, SSRs, tandem repeats, SNPs, indels, hotspots of nucleotide polymorphism, codon Ka/Ks ratios and RNA editing sites aiming to investigate evolutionary traits possibly affected by distinct environments. Our analyses revealed 303 SNPs, 91 indels, and 82 polymorphic SSRs among both species. Curiously, the narrow correlation among localization of repetitive sequences and indels strongly suggests that replication slippage is involved in plastid DNA mutations in Euterpe. Moreover, most non-synonymous substitutions represent amino acid variants in E. edulis that evolved specifically or in a convergent manner across the palm phylogeny. Amino acid variants observed in several plastid proteins in E. edulis were also identified as positive signatures across palm phylogeny. The higher incidence of specific amino acid changes in plastid genes of E. edulis in comparison with E. oleracea probably configures adaptive genetic variations determined by vicariant speciation. Our data indicate that the environment generates a selective pressure on the plastome making it more adapted to specific conditions.

Evaluating insect-host interactions as a driver of species divergence in palm flower weevils.

Plants and their specialized flower visitors provide valuable insights into the evolutionary consequences of species interactions. In particular, antagonistic interactions between insects and plants have often been invoked as a major driver of diversification. Here we use a tropical community of palms and their specialized insect flower visitors to test whether antagonisms lead to higher population divergence. Interactions between palms and the insects visiting their flowers range from brood pollination to florivory and commensalism, with the latter being species that feed on decaying-and presumably undefended-plant tissues. We test the role of insect-host interactions in the early stages of diversification of nine species of beetles sharing host plants and geographical ranges by first delimiting cryptic species and then using models of genetic isolation by environment. The degree to which insect populations are structured by the genetic divergence of plant populations varies. A hierarchical model reveals that this variation is largely uncorrelated with the kind of interaction, showing that antagonistic interactions are not associated with higher genetic differentiation. Other aspects of host use that affect plant-associated insects regardless of the outcomes of their interactions, such as sensory biases, are likely more general drivers of insect population divergence.

Higher evolutionary rates in life-history traits in insular than in mainland palms.

Isolated islands, due to the reduced interspecific competition compared to mainland habitats, present ecological opportunities for colonizing lineages. As a consequence, island lineages may be expected to experience higher rates of trait evolution than mainland lineages. However, island effects on key life-history traits of vascular plants remain underexplored at broad spatiotemporal scales, even for emblematic island clades such as palms. Here, we used phylogenetic comparative methods to evaluate potential differences in size and macroevolutionary patterns of height and fruit diameter among mainland, continental, and volcanic island palms. Further, phylogenetic beta-diversity was used to determine if lineage turnover supported an adaptive radiation scenario on volcanic islands. Volcanic island palms were taller than their continental island and mainland counterparts, whereas continental island palms exhibited smaller fruit size. Height and fruit size of palms evolved under evolutionary constraints towards an optimal value. However, scenarios of adaptive radiation and niche conservatism were not supported for the height and fruit size of volcanic and mainland palm clades, respectively, as expected. Instead, continental island palms exhibited higher evolutionary rates for height and fruit size. Insular palm assemblages (continental and volcanic) are composed of unique lineages. Beyond representing evolutionary sources of new palm lineages, our results demonstrate that insular habitats are important in shaping palm trait diversity. Also, the higher phenotypic evolutionary rates of continental island palms suggest disparate selection pressures on this habitat type, which can be an important driver of trait diversification over time. Taken together, these results stress the importance of insular habitats for conservation of functional, phylogenetic, and taxonomic diversity of palms.

First Study on the Oxidative Stability and Elemental Analysis of Babassu (Attalea speciosa) Edible Oil Produced in Brazil Using a Domestic Extraction Machine.

Interest in edible oil extraction processes is growing interest because the final nutritional quality of the extracted oil depends on the procedure used to obtain ir. In this context, a domestic cold oil press machine is a valuable tool that avoids the use of chemicals during oil extraction, in an environmentally friendly way. Although babassu (Attalea speciosa) oil is economically important in several Brazilian regions due to its nutritional and healthy features, few studies have been conducted on the chemical composition and stability of babassu oils extracted by cold pressing. Babassu oil's major constituents are saturated fatty acids (~86.42%), with the most prevalent fatty acids being lauric (~47.40%), myristic (15.64%), and oleic (~11.28%) acids, respectively, within the recommended range by Codex Alimentarius, presenting atherogenicity and thrombogenicity indexes favorable for human consumption. Peroxide value, Rancimat, and TGA/DSC results indicated that babassu oil is stable to oxidation. Also, macro- (Na, K, Ca, Mg, P) and micro-elements (Fe, Mn, Cr, Se, Al, and Zn) of babassu oil were determined, revealing levels below the tolerable upper intake level (ULs) for adults. These findings demonstrated that cold-press extraction using a domestic machine yielded a high-quality oil that kept oil chemical composition stable to oxidation with natural antioxidants.

Phylogeny, historical biogeography and diversification rates in an economically important group of Neotropical palms: Tribe Euterpeae.

Tribe Euterpeae is an economically and ecologically important group of Neotropical palms (Arecaceae). Some species are hyperdominant in the Neotropics, and many constitute a good source of revenue. To reconstruct the biogeographical history and diversification of the Euterpeae, we inferred a robust dated molecular phylogenetic hypothesis including 82% of the species sequenced for five DNA regions (trnD-trnT, CISP4, WRKY6, RPB2, and PHYB). Ancestral range was estimated using all models available in BioGeoBEARS and Binary State Speciation and Extinction analysis was used to evaluate the association of biome and inflorescence type with diversification rates. All intergeneric relationships were resolved providing insight on the taxonomic controversy of Jessenia, Euterpe and Prestoea. Three widely distributed Neotropical species were non-monophyletic, inviting a revision of species circumscriptions. The Euterpeae started its diversification in the mid Eocene (40 Mya), with most species-level divergence events occurring in the last 10 million years. Four colonization events from Central to South America were inferred. Different diversification rates were associated with biomes. Lowland rainforest was inferred as the ancestral biome of Euterpeae, attesting to the importance of lowland adapted lineages on the assembly of the montane flora. The two-fold higher speciation rate for montane taxa (compared with lowland rainforest taxa) was contemporaneous to the Andean orogenic uplift. The specialized beetle pollination of Oenocarpus with its hippuriform (horsetail shape) inflorescence was not associated with diversification rates in Euterpeae.

Soil fertility and flood regime are correlated with phylogenetic structure of Amazonian palm communities.

BACKGROUND AND AIMS: Identifying the processes that generate and maintain biodiversity requires understanding of how evolutionary processes interact with abiotic conditions to structure communities. Edaphic gradients are strongly associated with floristic patterns but, compared with climatic gradients, have received relatively little attention. We asked (1) How does the phylogenetic composition of palm communities vary along edaphic gradients within major habitat types? and (2) To what extent are phylogenetic patterns determined by (a) habitat specialists, (b) small versus large palms, and (c) hyperdiverse genera? METHODS: We paired data on palm community composition from 501 transects of 0.25 ha located in two main habitat types (non-inundated uplands and seasonally inundated floodplains) in western Amazonian rain forests with information on soil chemistry, climate, phylogeny and metrics of plant size. We focused on exchangeable base concentration (cmol+ kg-1) as a metric of soil fertility and a floristic index of inundation intensity. We used a null model approach to quantify the standard effect size of mean phylogenetic distance for each transect (a metric of phylogenetic community composition) and related this value to edaphic variables using generalized linear mixed models, including a term for spatial autocorrelation. KEY RESULTS: Overall, we recorded 112 008 individuals belonging to 110 species. Palm communities in non-inundated upland transects (but not floodplain transects) were more phylogenetically clustered in areas of low soil fertility, measured as exchangeable base concentration. In contrast, floodplain transects with more severe flood regimes (as inferred from floristic structure) tended to be phylogenetically clustered. Nearly half of the species recorded (44 %) were upland specialists while 18 % were floodplain specialists. In both habitat types, phylogenetic clustering was largely due to the co-occurrence of small-sized habitat specialists belonging to two hyperdiverse genera (Bactris and Geonoma). CONCLUSIONS: Edaphic conditions are associated with the phylogenetic community structure of palms across western Amazonia, and different factors (specifically, soil fertility and inundation intensity) appear to underlie diversity patterns in non-inundated upland versus floodplain habitats. By linking edaphic gradients with palm community phylogenetic structure, our study reinforces the need to integrate edaphic conditions in eco-evolutionary studies in order to better understand the processes that generate and maintain tropical forest diversity. Our results suggest a role for edaphic niche conservatism in the evolution and distribution of Amazonian palms, a finding with potential relevance for other clades.

Anatomical modifications of Butia capitata propagated under colored shade nets.

The jelly palm plant [Butia capitata (Martius) Beccari] is a native palm of the Cerrado biome used for many purposes in northern Minas Gerais State, Brazil. Dormancy is common in palm seeds, resulting in slow and uneven germination that may take years to complete. Modification in the growth pattern, anatomical parameters, physiological and biochemical characteristics of the plant can be verified due to changes in the light spectrum transmitted through colored shade nets used. Therefore, the objective of this study is to evaluate the effect of colored shade nets on the leaf and root anatomy of the jelly palm plant. The experiment was performed in a completely randomized design, with five treatments, ten replicates and eight plants per replicate, totaling 400 plants. Four colored photo-converter nets with 50% shading and different radiation proportions were employed: white (985 µmol.m-2.s-1), red (327 µmol.m-2.s-1), black (433 µmol.m-2.s-1) and silver (405 µmol.m-2.s-1). The plants cultivated under direct sunlight (1000 µmol.m-2.s-1) were considered as the control group. Leaf and root anatomical analysis was performed on 10 plants per treatment. It is possible to conclude that the colored shade nets caused changes in leaf and root anatomy of the jelly palm plant (Butia capitata).

The road to evolutionary success: insights from the demographic history of an Amazonian palm.

Evolutionary success, as demonstrated by high abundance and a wide geographical range, is related to genetic variation and historical demography. Here we assess how climatic change during the Quaternary influenced the demography and distribution of the Neotropical swamp palm Mauritia flexuosa. Using microsatellite loci and coalescent analyses we examined how demographical dynamics affected genetic diversity, effective population size and connectivity through time and space. Mauritia flexuosa presents significant genetic differentiation between the Amazonian and Cerrado biomes and among different river basins. Amazonian lineages are ancient compared to lineages from the Cerrado, a pattern corroborated using the fossil pollen record, where the species was absent from the Cerrado during the cold and dry periods of the last glacial cycles, then returned during the wet, interglacial phases. Coalescent simulations show that the pattern of observed genetic diversity for M. flexuosa is most likely due to a range retraction during the Last Glacial Maximum, leading to multiple refugia and resulting in high differentiation between Amazonian and Cerrado biomes. Isolation-by-distance and by-environment also shaped the distribution and evolutionary success of M. flexuosa. Our study provides new insights into the historical factors that affected geographical distribution and structure genetic diversity, contributing to long-term evolutionary success.

Population genetic structure of three species in the genus Astrocaryum G. Mey. (Arecaceae).

We assessed the level and distribution of genetic diversity in three species of the economically important palm genus Astrocaryum located in Pará State, in northern Brazil. Samples were collected in three municipalities for Astrocaryum aculeatum: Belterra, Santarém, and Terra Santa; and in two municipalities for both A. murumuru: Belém and Santo Antônio do Tauá and A. paramaca: Belém and Ananindeua. Eight microsatellite loci amplified well and were used for genetic analysis. The mean number of alleles per locus for A. aculeatum, A. murumuru, and A. paramaca were 2.33, 2.38, and 2.06, respectively. Genetic diversity was similar for the three species, ranging from HE = 0.222 in A. aculeatum to HE = 0.254 in A. murumuru. Both FST and AMOVA showed that most of the genetic variation was found within populations for all three species, but high genetic differentiation among populations was found for A. aculeatum. Three loci were not in Hardy-Weinberg equilibrium, with populations of A. paramaca showing a tendency for the excess of heterozygotes (FIS = -0.144). Gene flow was high for populations of A. paramaca (Nm = 19.35). Our results suggest that the genetic diversity within populations followed the genetic differentiation among populations due to high gene flow among the population. Greater geographic distances among the three collection sites for A. aculeatum likely hampered gene flow for this species.

Tocopherols, Tocomonoenols, and Tocotrienols in Oils of Costa Rican Palm Fruits: A Comparison between Six Varieties and Chemical versus Mechanical Extraction.

Palm oil is one of the richest sources of tocotrienols and may contain other non-tocopherol vitamin E congeners. The vitamin E profiles of fully ripened fruit mesocarp of three Elaeis guineensis, two Elaeis oleifera, and one hybrid O × G palm fruit genotypes from Costa Rica were analyzed by high-performance liquid chromatography with fluorescence detection and gas chromatography-mass spectrometry after mechanical extraction by a screw press and chemical extraction with hexane. γ-Tocotrienol, α-tocotrienol, and α-tocopherol were the most abundant tocochromanols, while other tocopherols (ß-tocopherol, γ-tocopherol, and δ-tocopherol) and α-tocomonoenol were detected at minor concentrations. Significant differences in vitamin E profiles between genotypes were observed, and the variety E. oleifera Quepos (CB9204) had by far the highest content of total tocotrienols (890 µg/g of oil) and total vitamin E (892 µg/g of oil). Chemical extraction with hexane afforded up to 2.5-fold higher vitamin E yields than screw press extraction. α-Tocomonoenol co-eluted with γ-tocopherol in reversed-phase high-performance liquid chromatography analyses and is a possible source of error in the quantification of γ-tocopherol in foods.

Transferability of microsatellite markers in Syagrus coronata (Mart.) Becc. (Arecaceae), an iconic palm tree from the Brazilian semiarid region.

The licuri palm Syagrus coronata plays a key role in the ecology and economy of Brazilian semiarid region. Nonetheless, genetic data about populations of this species are absent even though the intensive and uncontrolled exploitation since colonial periods has threatened the sustainability and viability of licuri populations. Therefore, we attempted to test the efficacy of transferability of microsatellite loci isolated from three palm tree species to S. coronata to analyze the population of this species throughout their range. A set of 19 heterologous microsatellite loci was tested in three native populations of S. coronata from the State of Bahia, northeastern Brazil, which amplified using distinct annealing temperatures (50°-60°C). Based on the 10 most polymorphic loci, the selected populations exhibited a mean number of alleles per locus of 9.8, and high genetic diversity values since the expected heterozygosity ranged from 0.573 to 0.754, while the observed heterozygosity varied from 0.785 to 1.000. In conclusion, the tested loci are transferrable and highly efficient to population studies in S. coronata, thus minimizing the lack of species-specific loci to the genetic monitoring of licuri populations.

Foreign Plastid Sequences in Plant Mitochondria are Frequently Acquired Via Mitochondrion-to-Mitochondrion Horizontal Transfer.

Angiosperm mitochondrial genomes (mtDNA) exhibit variable quantities of alien sequences. Many of these sequences are acquired by intracellular gene transfer (IGT) from the plastid. In addition, frequent events of horizontal gene transfer (HGT) between mitochondria of different species also contribute to their expanded genomes. In contrast, alien sequences are rarely found in plastid genomes. Most of the plant-to-plant HGT events involve mitochondrion-to-mitochondrion transfers. Occasionally, foreign sequences in mtDNAs are plastid-derived (MTPT), raising questions about their origin, frequency, and mechanism of transfer. The rising number of complete mtDNAs allowed us to address these questions. We identified 15 new foreign MTPTs, increasing significantly the number of those previously reported. One out of five of the angiosperm species analyzed contained at least one foreign MTPT, suggesting a remarkable frequency of HGT among plants. By analyzing the flanking regions of the foreign MTPTs, we found strong evidence for mt-to-mt transfers in 65% of the cases. We hypothesize that plastid sequences were initially acquired by the native mtDNA via IGT and then transferred to a distantly-related plant via mitochondrial HGT, rather than directly from a foreign plastid to the mitochondrial genome. Finally, we describe three novel putative cases of mitochondrial-derived sequences among angiosperm plastomes.

Papel de los mamíferos en los procesos de dispersión y depredación de semillas de Mauritia flexuosa (Arecaceae) en la Amazonía colombiana / Role of mammals on seed dispersal and predation processes of Mauritia flexuosa (Arecaceae) in the Colombian Amazon

ResumenLos mamíferos y las palmas son importantes elementos de la fauna y flora en el neotrópico. Por lo tanto, las interacciones entre ellos son una de las relaciones ecológicas más significativas en estos ecosistemas. Con el fin de identificar la importancia de los mamíferos que consumen los frutos de la palma Mauritia flexuosa y determinar su participación en la dispersión y depredación de sus semillas, se instalaron trampas cámara en frente de cúmulos de semillas caídas y de racimos con frutos. Con los videos obtenidos se preparó un catálogo de especies. Además, se clasificaron y cuantificaron los comportamientos de forrajeo exhibidos. También se utilizaron dos tratamientos de exclusión con tres repeticiones. En el tratamiento abierto se permitió el libre acceso de mamíferos pequeños, medianos y grandes, mientras que en el tratamiento semi-abierto se cercó la parcela con malla metálica dejando cuatro aberturas en la base para permitir el acceso y evaluar la remoción de semillas por mamíferos pequeños y medianos. Se registraron 19 especies de mamíferos, de las cuales nueve se alimentaron de los frutos de la palma y cinco de éstas fueron dispersoras de semillas. Se reporta por primera vez el consumo de los frutos de M. flexuosa por pare de Atelocynus microtis. La especie con mayor importancia relativa fue Dasyprocta fuliginosa, que mostro el mayor porcentaje de dispersión de semillas (63.5%) en comparación con las otras especies. Tayassu pecari fue identificado como consumidor in situ, porque consume hasta el 45.3 % de las semillas, sin dispersarlas. El número de semillas consumidas in situ en el tratamiento abierto mostró diferencias significativas con respecto al tratamiento semi-abierto, lo que sugiere una mayor participación de los mamíferos grandes en este proceso. En conclusión, los frutos de M. flexuosa son una fuente alimenticia importante para la comunidad de mamíferos. Además, el consumo de las semillas bajo la copa de la palma madre, el cual favorece la mortalidad de las mismas por efectos densodependientes, es proporcionalmente mayor que la dispersión. Generalmente la presión ejercida por las especies frugívoras sobre las semillas puede moldear las estrategias reproductivas de las especies de plantas. Sin embargo se deben realizar investigaciones sobre dispersión efectiva, distancias de dispersión y efectos demográficos, para determinar la función específica de los mamíferos medianos y grandes en la ecología de esta palma.

AbstractMammals and palms are important elements of fauna and flora in the Neotropics, and their interactions, such as fruit consumption and seed dispersal, are one of the most important ecological relationships in these ecosystems. The main objective of this study was to identify the relative importance of mammals in the dispersal and predation of Mauritia flexuosa palm fruits. We installed camera-traps in front of palm fallen seeds and clusters with fruits. A catalog of species was prepared with the recorded videos and the foraging behaviors exhibited were classified and identified. In addition, two exclusion treatments with three repetitions each were used. In the semi-open treatment, a plot was fenced with metal mesh leaving four openings in order to allow access only to small and medium sized mammals, while in the open treatment, the small, medium and large sized mammals had free access. In both cases, seed removal was evaluated. We recorded a total of 19 species of mammals, nine of which fed on palm fruits and the other five were seed dispersers. We reported for the first time the consumption of Mauritia flexuosa fruits by Atelocynus microtis. The species with the highest relative importance was Dasyprocta fuliginosa, which showed the highest percentage of seed dispersal (63.5%) compared to the other species. Tayassu peccary was identified as an in situ consumer, eating 45.3% of seeds without dispersing them. The number of seeds consumed in situ in the open treatment showed significant differences regarding the semi-open treatment, suggesting greater involvement of large mammals in this process. In conclusion, the fruits of M. flexuosa are an important food source for the local mammal community. Additionally, the consumption of seeds under the canopy of the mother palm is proportionally greater than their dispersion. Generally, the pressure of frugivorous species over seeds may determine the reproductive strategies of plants. However, research on effective dispersion, dispersal distances and demographic impact should be conducted to determine the specific role of medium and large sized mammals in the ecology of this palm. Rev. Biol. Trop. 64 (1): 5-15. Epub 2016 March 01.

[Role of mammals on seed dispersal and predation processes of Mauritia flexuosa (Arecaceae) in the Colombian Amazon]. / Papel de los mamíferos en los procesos de dispersión y depredación de semillas de Mauritia flexuosa (Arecaceae) en la Amazonía colombiana.

Mammals and palms are important elements of fauna and flora in the Neotropics, and their interactions, such as fruit consumption and seed dispersal, are one of the most important ecological relationships in these ecosystems. The main objective of this study was to identify the relative importance of mammals in the dispersal and predation of Mauritia flexuosa palm fruits. We installed camera-traps in front of palm fallen seeds and clusters with fruits. A catalog of species was prepared with the recorded videos and the foraging behaviors exhibited were classified and identified. In addition, two exclusion treatments with three repetitions each were used. In the semi-open treatment, a plot was fenced with metal mesh leaving four open- ings in order to allow access only to small and medium sized mammals, while in the open treatment, the small, medium and large sized mammals had free access. In both cases, seed removal was evaluated. We recorded a total of 19 species of mammals, nine of which fed on palm fruits and the other five were seed dispersers. We reported for the first time the consumption of Mauritia flexuosa fruits by Atelocynus microtis. The species with the highest relative importance was Dasyprocta fuliginosa, which showed the highest percentage of seed dispersal (63.5%) compared to the other species. Tayassu peccary was identified as an in situ consumer, eating 45.3% of seeds without dispersing them. The number of seeds consumed in situ in the open treatment showed significant differences regarding the semi-open treatment, suggesting greater involvement of large mammals in this process. In conclusion, the fruits of M. flexuosa are an important food source for the local mammal com- munity. Additionally, the consumption of seeds under the canopy of the mother palm is proportionally greater than their dispersion. Generally, the pressure of frugivorous species over seeds may determine the reproductive strategies of plants. However, research on effective dispersion, dispersal distances and demographic impact should be conducted to determine the specific role of medium and large sized mammals in the ecology of this palm.

Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers.

Genetic diversity and patterns of population structure of the 94 oil palm lines were investigated using species-specific simple sequence repeat (SSR) markers. We designed primers for 63 SSR loci based on their flanking sequences and conducted amplification in 94 oil palm DNA samples. The amplification result showed that a relatively high level of genetic diversity was observed between oil palm individuals according a set of 21 polymorphic microsatellite loci. The observed heterozygosity (Ho) was 0.3683 and 0.4035, with an average of 0.3859. The Ho value was a reliable determinant of the discriminatory power of the SSR primer combinations. The principal component analysis and unweighted pair-group method with arithmetic averaging cluster analysis showed the 94 oil palm lines were grouped into one cluster. These results demonstrated that the oil palm in Hainan Province of China and the germplasm introduced from Malaysia may be from the same source. The SSR protocol was effective and reliable for assessing the genetic diversity of oil palm. Knowledge of the genetic diversity and population structure will be crucial for establishing appropriate management stocks for this species.