ABSTRACT
Recently, a novel antiviral compound (K22) that inhibits replication of a broad range of animal and human coronaviruses was reported to interfere with viral RNA synthesis by impairing double-membrane vesicle (DMV) formation (Lundin et al., 2014). Here we assessed potential antiviral activities of K22 against a range of viruses representing two (sub)families of the order Nidovirales, the Arteriviridae (porcine reproductive and respiratory syndrome virus [PRRSV], equine arteritis virus [EAV] and simian hemorrhagic fever virus [SHFV]), and the Torovirinae (equine torovirus [EToV] and White Bream virus [WBV]). Possible effects of K22 on nidovirus replication were studied in suitable cell lines. K22 concentrations significantly decreasing infectious titres of the viruses included in this study ranged from 25 to 50⯵M. Reduction of double-stranded RNA intermediates of viral replication in nidovirus-infected cells treated with K22 confirmed the anti-viral potential of K22. Collectively, the data show that K22 has antiviral activity against diverse lineages of nidoviruses, suggesting that the inhibitor targets a critical and conserved step during nidovirus replication.
Subject(s)
Antiviral Agents/pharmacology , Arterivirus/drug effects , Benzamides/pharmacology , Coronaviridae/drug effects , Equartevirus/drug effects , Piperidines/pharmacology , Porcine respiratory and reproductive syndrome virus/drug effects , Torovirus/drug effects , Animals , Arterivirus/genetics , Arterivirus/growth & development , Arterivirus/metabolism , Carps , Cell Line , Chlorocebus aethiops , Coronaviridae/genetics , Coronaviridae/growth & development , Coronaviridae/metabolism , Epithelial Cells/drug effects , Epithelial Cells/virology , Equartevirus/genetics , Equartevirus/growth & development , Equartevirus/metabolism , Mesocricetus , Porcine respiratory and reproductive syndrome virus/genetics , Porcine respiratory and reproductive syndrome virus/growth & development , Porcine respiratory and reproductive syndrome virus/metabolism , RNA, Double-Stranded/antagonists & inhibitors , RNA, Double-Stranded/biosynthesis , RNA, Double-Stranded/genetics , RNA, Viral/antagonists & inhibitors , RNA, Viral/biosynthesis , RNA, Viral/genetics , Torovirus/genetics , Torovirus/growth & development , Torovirus/metabolism , Virus Replication/drug effectsABSTRACT
UNLABELLED: Simian hemorrhagic fever (SHF) is a highly lethal disease in captive macaques. Three distinct arteriviruses are known etiological agents of past SHF epizootics, but only one, simian hemorrhagic fever virus (SHFV), has been isolated in cell culture. The natural reservoir(s) of the three viruses have yet to be identified, but African nonhuman primates are suspected. Eleven additional divergent simian arteriviruses have been detected recently in diverse and apparently healthy African cercopithecid monkeys. Here, we report the successful isolation in MARC-145 cell culture of one of these viruses, Kibale red colobus virus 1 (KRCV-1), from serum of a naturally infected red colobus (Procolobus [Piliocolobus] rufomitratus tephrosceles) sampled in Kibale National Park, Uganda. Intramuscular (i.m.) injection of KRCV-1 into four cynomolgus macaques (Macaca fascicularis) resulted in a self-limiting nonlethal disease characterized by depressive behavioral changes, disturbance in coagulation parameters, and liver enzyme elevations. In contrast, i.m. injection of SHFV resulted in typical lethal SHF characterized by mild fever, lethargy, lymphoid depletion, lymphoid and hepatocellular necrosis, low platelet counts, increased liver enzyme concentrations, coagulation abnormalities, and increasing viral loads. As hypothesized based on the genetic and presumed antigenic distance between KRCV-1 and SHFV, all four macaques that had survived KRCV-1 injection died of SHF after subsequent SHFV injection, indicating a lack of protective heterotypic immunity. Our data indicate that SHF is a disease of macaques that in all likelihood can be caused by a number of distinct simian arteriviruses, although with different severity depending on the specific arterivirus involved. Consequently, we recommend that current screening procedures for SHFV in primate-holding facilities be modified to detect all known simian arteriviruses. IMPORTANCE: Outbreaks of simian hemorrhagic fever (SHF) have devastated captive Asian macaque colonies in the past. SHF is caused by at least three viruses of the family Arteriviridae: simian hemorrhagic fever virus (SHFV), simian hemorrhagic encephalitis virus (SHEV), and Pebjah virus (PBJV). Nine additional distant relatives of these three viruses were recently discovered in apparently healthy African nonhuman primates. We hypothesized that all simian arteriviruses are potential causes of SHF. To test this hypothesis, we inoculated cynomolgus macaques with a highly divergent simian arterivirus (Kibale red colobus virus 1 [KRCV-1]) from a wild Ugandan red colobus. Despite being only distantly related to red colobuses, all of the macaques developed disease. In contrast to SHFV-infected animals, KRCV-1-infected animals survived after a mild disease presentation. Our study advances the understanding of an important primate disease. Furthermore, our data indicate a need to include the full diversity of simian arteriviruses in nonhuman primate SHF screening assays.
Subject(s)
Arterivirus Infections/veterinary , Arterivirus/isolation & purification , Arterivirus/pathogenicity , Colobus/virology , Hemorrhagic Fevers, Viral/veterinary , Macaca fascicularis/virology , Monkey Diseases/virology , Animals , Arterivirus/genetics , Arterivirus/growth & development , Arterivirus Infections/immunology , Arterivirus Infections/physiopathology , Arterivirus Infections/virology , Cell Line , Hemorrhagic Fevers, Viral/immunology , Hemorrhagic Fevers, Viral/physiopathology , Hemorrhagic Fevers, Viral/virology , Liver/chemistry , Liver/enzymology , Male , Monkey Diseases/immunology , Monkey Diseases/physiopathology , Uganda , Viral LoadABSTRACT
We developed an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against PRRS virus in swine sera. The ELISA antigen was prepared from MARC-145 cells infected with PRRS virus. The results from serial serum samples from experimentally infected and random swine indicated that the ELISA was more sensitive than indirect fluorescent and immunoperoxidase monolayer assays. Since the ELISA enables many sera to be simultaneously and rapidly tested, it was useful for detection antibodies against PRRS virus in swine sera.
Subject(s)
Antibodies, Viral/blood , Arterivirus Infections/veterinary , Arterivirus/immunology , Swine Diseases , Animals , Antigens, Viral/immunology , Arterivirus/growth & development , Arterivirus Infections/diagnosis , Arterivirus Infections/immunology , Cell Line , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique, Indirect , Immunoenzyme Techniques , Predictive Value of Tests , Respiratory Tract Diseases/diagnosis , Respiratory Tract Diseases/immunology , Respiratory Tract Diseases/veterinary , Sensitivity and Specificity , Swine , SyndromeABSTRACT
OBJECTIVE: To determine whether porcine reproductive and respiratory syndrome virus plaque variants vary in their pathogenicity in causing late-term reproductive failure. DESIGN: Four groups of 2 sows each at 86 days of gestation were inoculated intranasally with PRRSV small (MN-Hs) and large (MN-HI) plaque variants, field isolate, and cell culture medium, respectively. In addition, 2 sows each at 86 days of gestation were inoculated intranasally or IM with MN-Hs virus. ANIMALS: 14 pregnant sows. PROCEDURE: Inoculated sows were allowed to deliver at term, and each litter was examined for clinical abnormality and presence of virus. RESULTS: Two sows infected with MN-Hs virus delivered 14 live and 5 dead pigs, whereas 2 sows infected with MN-HI virus delivered 0 live and 25 dead pigs. Two sows inoculated with a field isolate (MN-W) delivered 10 live and 20 dead pigs. Two control sows had 26 normal fetuses at slaughter at 107 days of gestation. Virus was isolated from 16 (66.7%) of 24 liveborn pigs, 9 (64.3%) of 14 stillborn pigs, and 3 (12.0%) of 25 mummified fetuses of the 6 infected sows. Subsequently, 4 MN-Hs-infected sows delivered 40 live and 11 dead pigs. CONCLUSIONS: Marked difference in the pathogenicity in pregnant sows between porcine reproductive and respiratory syndrome virus strains was documented. The MN-Hs virus is considered to be of low pathogenicity, but the other viruses are highly pathogenic for late-term pregnant sows.
Subject(s)
Arterivirus Infections/veterinary , Arterivirus/genetics , Pregnancy Complications, Infectious/veterinary , Respiratory Tract Infections/veterinary , Swine Diseases , Animals , Arterivirus/growth & development , Arterivirus/isolation & purification , Cell Line , Chlorocebus aethiops , Female , Genetic Variation , Kidney , Pregnancy , Pregnancy Complications, Infectious/virology , Respiratory Tract Infections/virology , Species Specificity , Swine , Syndrome , Viral Plaque AssayABSTRACT
Infection of porcine alveolar macrophages by the porcine reproductive and respiratory syndrome virus (PRRSV) was significantly enhanced in vitro by antibody raised against the PRRSV isolate ISU-P (p < 0.01). Increased yields and infection rates were highly correlated (r = 0.95) and the ratio of yield to infection rate was greater than 1.4, suggesting that more than one mechanism was responsible for enhanced infection. Antibody-dependent enhancement (ADE) of infection was also demonstrated in vivo using a completely randomized block design (n = 16). The mean level and duration of viremia were greater (p < 0.05) in pigs injected with subneutralizing amounts of PRRSV-specific IgG prior to virus challenge than in control pigs injected with normal IgG. In contrast, virus replication was significantly (p < 0.01) inhibited in pigs with neutralizing antibody titers of 4 log2. The period of time that subneutralizing levels of antibody can persist and contribute to ADE of PRRSV infection was estimated in 4 pigs injected with PRRSV-specific IgG to yield an initial neutralizing antibody titer of 3.8 log2. Neutralizing activity declined to undetectable levels by day 37 postinjection (PI). ADE activity was first detected in undiluted sera on day 20 PI and persisted through day 62 PI. Western immunoblot analysis of sera collected between days 37 and 62 PI detected antibodies specific for the 15-kDa nucleocapsid and 26-kDa glycosylated envelope proteins. These results strongly suggest that ADE has the potential to contribute to the pathogenesis of PRRSV infection and is mediated by antibody specific for the 26-kDa envelope protein.
Subject(s)
Antibodies, Viral/immunology , Arterivirus Infections/veterinary , Arterivirus/immunology , Swine Diseases/virology , Animals , Arterivirus/drug effects , Arterivirus/growth & development , Arterivirus Infections/immunology , Arterivirus Infections/virology , Cells, Cultured , Macrophages, Alveolar/cytology , Macrophages, Alveolar/immunology , Neutralization Tests , Staphylococcal Protein A/pharmacology , Swine , Swine Diseases/immunology , Viremia/immunology , Viremia/veterinaryABSTRACT
Lelystad virus (LV) is an enveloped positive-stranded RNA virus, which causes abortions and respiratory disease in pigs. The complete nucleotide sequence of the genome of LV has been determined. This sequence is 15.1 kb in length and contains a poly(A) tail at the 3' end. Open reading frames that might encode the viral replicases (ORFs 1a and 1b), membrane-associated proteins (ORFs 2 to 6) and the nucleocapsid protein (ORF7) have been identified. Sequence comparisons have indicated that LV is distantly related to the coronaviruses and toroviruses and closely related to lactate dehydrogenase-elevating virus (LDV) and equine arteritis virus (EAV). A 3' nested set of six subgenomic RNAs is produced in LV-infected alveolar lung macrophages. These subgenomic RNAs contain a leader sequence, which is derived from the 5' end of the viral genome. Altogether, these data show that LV is closely related evolutionarily to LDV and EAV, both members of a recently proposed family of positive-stranded RNA viruses, the Arteriviridae.