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1.
J Neuroimmunol ; 332: 112-125, 2019 07 15.
Article in English | MEDLINE | ID: mdl-31005712

ABSTRACT

Traumatic brain injury (TBI) is the leading cause of death and disability in young adults in the developed world. The accuracy of early outcome-prediction remains poor even when all known prognostic factors are considered, suggesting important currently unidentified variables. In addition, whilst survival and neurological outcomes have improved markedly with the utilisation of therapies that optimise physiology, no treatments specifically modulate the underlying pathophysiology. The immunological response to TBI represents both a potential contributor to outcome heterogeneity and a therapeutically tractable component of the acute disease process. Furthermore, chronic inflammation has been linked with neurodegeneration, and may mark a bridge between acute brain injury and the subsequent neurodegenerative process seen in a proportion of patients following TBI. Given the complexity of the immune response and its varying functions ranging from repair of injury to bystander damage of healthy tissue, attempts at immunomodulatory intervention must necessarily be highly targeted towards the maladaptive facets of the inflammatory process. In this review we aim to provide an integrated description of the immunological processes triggered by TBI in both humans and animal models, in particular considering the interplay between the innate immune system, danger-associated molecular patterns and loss of self-tolerance leading to adaptive autoimmunity.


Subject(s)
Brain Injuries, Traumatic/immunology , Adaptive Immunity , Alarmins/immunology , Animals , Astrocytes/immunology , Autoantibodies/biosynthesis , Autoantibodies/immunology , Autoimmune Diseases of the Nervous System/etiology , Autoimmune Diseases of the Nervous System/immunology , Autoimmune Diseases of the Nervous System/prevention & control , Brain Damage, Chronic/etiology , Brain Damage, Chronic/prevention & control , Brain Injuries, Traumatic/complications , Cytokines/antagonists & inhibitors , Cytokines/biosynthesis , Cytokines/immunology , Humans , Immunity, Innate , Immunomodulation , Lymphocyte Subsets/immunology , Mice , Mice, Knockout , Microglia/immunology , Neurodegenerative Diseases/etiology , Neurodegenerative Diseases/immunology , Neurodegenerative Diseases/prevention & control , Neutrophils/immunology , Pattern Recognition, Automated , Rats , Time Factors
2.
J Neuroimmunol ; 286: 48-58, 2015 Sep 15.
Article in English | MEDLINE | ID: mdl-26298324

ABSTRACT

Multiple sclerosis (MS) is a neurodegenerative disease resulting from an autoimmune attack on the axon-myelin unit. A female MS bias becomes evident after puberty and female incidence has tripled in the last half-century, implicating a female sex hormone interacting with a modifiable environmental factor. These aspects of MS suggest that many female MS cases may be preventable. Mechanistic knowledge of this hormone-environment interaction is needed to devise strategies to reduce female MS risk. We previously demonstrated that vitamin D3 (D3) deficiency increases and D3 supplementation decreases experimental autoimmune encephalomyelitis (EAE) risk in a female-biased manner. We also showed that D3 acts in an estrogen (E2)-dependent manner, since ovariectomy eliminated and E2 restored D3-mediated EAE protection. Here we probed the hypothesis that E2 and D3 interact synergistically within CD4(+) T cells to control T cell fate and prevent demyelinating disease. The E2 increased EAE resistance in wild-type (WT) but not T-Vdr(0) mice lacking Vdr gene function in CD4(+) T cells, so E2 action depended entirely on Vdr(+)CD4(+) T cells. The E2 levels were higher in WT than T-Vdr(0) mice, suggesting the Vdr(+)CD4(+) T cells produced E2 or stimulated its production. The E2 decreased Cyp24a1 and increased Vdr transcripts in T cells, prolonging the calcitriol half-life and increasing calcitriol responsiveness. The E2 also increased CD4(+)Helios(+)FoxP3(+) T regulatory (Treg) cells in a Vdr-dependent manner. Thus, CD4(+) T cells have a cooperative amplification loop involving E2 and calcitriol that promotes CD4(+)Helios(+)FoxP3(+) Treg cell development and is disrupted when the D3 pathway is impaired. The global decline in population D3 status may be undermining a similar cooperative E2-D3 interaction controlling Treg cell differentiation in women, causing a breakdown in T cell self tolerance and a rise in MS incidence.


Subject(s)
Autoimmune Diseases of the Nervous System/prevention & control , CD4-Positive T-Lymphocytes/metabolism , DNA-Binding Proteins/metabolism , Estrogens/administration & dosage , Forkhead Transcription Factors/metabolism , Receptors, Calcitriol/metabolism , T-Lymphocytes, Regulatory/metabolism , Transcription Factors/metabolism , Vitamin D/administration & dosage , Animals , Autoimmune Diseases of the Nervous System/chemically induced , Autoimmune Diseases of the Nervous System/genetics , Autoimmune Diseases of the Nervous System/pathology , CD4-Positive T-Lymphocytes/drug effects , DNA-Binding Proteins/genetics , Disease Models, Animal , Drug Synergism , Female , Forkhead Transcription Factors/genetics , Gene Expression Regulation, Developmental/drug effects , Gene Expression Regulation, Developmental/genetics , In Vitro Techniques , Male , Mice , Mice, Inbred C57BL , Mice, Transgenic , Myelin-Oligodendrocyte Glycoprotein/toxicity , Ovariectomy , Peptide Fragments/toxicity , Pregnancy , Receptors, Calcitriol/genetics , T-Lymphocytes, Regulatory/drug effects , Time Factors , Transcription Factors/genetics , Uterus/pathology
3.
Am J Alzheimers Dis Other Demen ; 30(3): 225-7, 2015 May.
Article in English | MEDLINE | ID: mdl-25100747

ABSTRACT

The Alzheimer's Association recently reported that a woman's estimated lifetime risk of developing Alzheimer's at age 65 is 1 in 6, compared to nearly 1 in 11 for a man (ie, female to male ratio 1.8). Based on female to male ratio, Alzheimer's disease could well be an autoimmune disorder. Like Alzheimer's, multiple sclerosis, an autoimmune inflammation of the central nervous system, has a female to male ratio of 2.3. Also based on female to male ratio, Alzheimer's resembles the autoimmune inflammatory disease rheumatoid arthritis, which has a female to male ratio of 2.7. The reasons for the female preponderance in autoimmune disease are unclear, but nonsteroidal anti-inflammatory drugs (NSAIDs) are widely and successfully employed to treat autoimmune anti-inflammatory disease and dramatically relieve symptoms. Moreover, oral NSAIDs consistently reduce the risk of Alzheimer's disease, although they have been totally ineffective as a treatment in multiple failed clinical trials. A basis for this failure might well be that the brain dose after oral administration is too small and not sufficiently early in the pathogenesis of the disorder. But NSAID brain dose could be significantly increased by delivering the NSAIDs intranasally.


Subject(s)
Administration, Intranasal , Alzheimer Disease/prevention & control , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Autoimmune Diseases of the Nervous System/prevention & control , Brain/drug effects , Aged , Alzheimer Disease/epidemiology , Autoimmune Diseases of the Nervous System/epidemiology , Female , Humans , Male
5.
J Neuroimmunol ; 263(1-2): 98-107, 2013 Oct 15.
Article in English | MEDLINE | ID: mdl-24029580

ABSTRACT

We report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (IRBP) 201-216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (EAU) in A/J mice. One mimicry epitope from Ehrlichia canis (EHC), designated EHC 44-59, induced cross-reactive T cells for IRBP 201-216 capable of producing T helper (Th)1 and Th17 cytokines, but failed to induce EAU in A/J mice. In addition, animals first primed with suboptimal doses of IRBP 201-216 and subsequently immunized with EHC 44-59 did not develop EAU; rather, the mimicry epitope prevented the disease induced by IRBP 201-216. However, alteration in the composition of EHC 44-59 by substituting alanine with valine at position 49, similar to the composition of IRBP 201-216, enabled the mimicry epitope to acquire uveitogenicity. The data provide new insights as to how microbes containing mimicry sequences for retinal antigens can prevent ocular inflammation by acting as naturally occurring altered peptide ligands.


Subject(s)
Autoimmune Diseases of the Nervous System/prevention & control , Ehrlichia canis/immunology , Ehrlichiosis/prevention & control , Molecular Mimicry/immunology , Retinitis/prevention & control , Uveitis/prevention & control , Amino Acid Sequence , Animals , Autoimmune Diseases of the Nervous System/immunology , Autoimmune Diseases of the Nervous System/microbiology , Cattle , Ehrlichia canis/genetics , Ehrlichiosis/immunology , Ehrlichiosis/microbiology , Eye Proteins/administration & dosage , Eye Proteins/genetics , Eye Proteins/metabolism , Female , Ligands , Mice , Mice, Inbred A , Molecular Sequence Data , Retinitis/immunology , Retinitis/microbiology , Retinol-Binding Proteins/administration & dosage , Retinol-Binding Proteins/genetics , Retinol-Binding Proteins/metabolism , Uveitis/immunology , Uveitis/microbiology
7.
J Clin Invest ; 118(10): 3271-3, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18802483

ABSTRACT

The liver has several unique immunological properties that affect T cell activation and immune regulation. Recent studies have uncovered opportunities for the treatment of genetic disease by directing expression of the functional therapeutic protein to hepatocytes. In a new study in this issue of the JCI, Lüth and colleagues demonstrate that hepatic expression of a brain protein is protective against neuroinflammatory disease in a mouse model of human MS (see the related article beginning on page 3403). Suppression of autoimmunity was dependent on transgene expression in the liver and was mediated by induction of antigen-specific CD4+CD25+Foxp3+ Tregs. These findings suggest that the introduction of antigens to the liver may have potential as a preventative or therapeutic intervention for autoimmune disease.


Subject(s)
Autoimmune Diseases of the Nervous System/prevention & control , Brain/immunology , Encephalomyelitis, Autoimmune, Experimental/immunology , Encephalomyelitis, Autoimmune, Experimental/therapy , Liver/immunology , Animals , Antigens/immunology , Autoimmune Diseases of the Nervous System/immunology , Disease Models, Animal , Encephalomyelitis, Autoimmune, Experimental/prevention & control , Gene Expression Regulation/immunology , Gene Transfer Techniques , Humans , Inflammation/immunology , Liver/metabolism , Mice , Mice, Transgenic , T-Lymphocytes, Regulatory/immunology
8.
Glia ; 56(15): 1625-36, 2008 Nov 15.
Article in English | MEDLINE | ID: mdl-18551629

ABSTRACT

T cell mediated immunotherapies are proposed for many cancers including malignant astrocytoma. As such therapies become more potent, but not necessarily more tumor-specific, the risk of collateral autoimmune damage to normal tissue increases. Tumors of the brain present significant challenges in this respect, as autoimmune destruction of brain tissue could have severe consequences. To investigate local immune reactivity toward a tumor-associated antigen in the brain, transgenic mice were generated that express a defined antigen (CW3 170-179) in astroglial cells. The resulting six transgenic mouse lines expressed the transgenic self-antigen in cells of the gastrointestinal tract and CNS compartments, or in the CNS alone. By challenging transgenic mice with tumor cells that express CW3, self/tumor-specific immune responses were visualized within a normal polyclonal T cell repertoire. A large expansion of the endogenous CW3 170-179-specific CD8 T cell population was observed in nontransgenic mice after both subcutaneous and intracerebral implantation of tumor cells. In contrast, CW3 170-179-specific immune responses were not observed in transgenic mice that exhibited extracerebral transgene expression. Importantly, in certain groups of mice in which transgene expression was restricted to the CNS, antigen-specific immune responses occurred when tumor was implanted subcutaneously, but not intracerebrally. This local immune tolerance in the brain was induced via peripheral (extrathymic) rather than central (thymic) tolerance mechanisms. Thus, this study highlights the role of regional immune regulation in the prevention of autoimmunity in the brain, and the potential impact of these mechanisms for brain tumor immunotherapy.


Subject(s)
Antigens, Neoplasm/immunology , Astrocytes/immunology , Astrocytoma/immunology , Brain Neoplasms/immunology , Brain/immunology , CD8-Positive T-Lymphocytes/immunology , Immune Tolerance/immunology , Animals , Antigens, Neoplasm/genetics , Astrocytes/pathology , Astrocytoma/physiopathology , Autoantigens/genetics , Autoantigens/immunology , Autoimmune Diseases of the Nervous System/genetics , Autoimmune Diseases of the Nervous System/immunology , Autoimmune Diseases of the Nervous System/prevention & control , Autoimmunity/genetics , Autoimmunity/immunology , Brain/pathology , Brain/physiopathology , Brain Neoplasms/physiopathology , Brain Tissue Transplantation , Immunotherapy/adverse effects , Immunotherapy/methods , Mice , Mice, Transgenic , Tumor Cells, Cultured
9.
J Exp Med ; 205(4): 811-23, 2008 Apr 14.
Article in English | MEDLINE | ID: mdl-18347102

ABSTRACT

The ELR(+) CXC chemokines CXCL1 and CXCL2 are up-regulated in the central nervous system (CNS) during multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). However, their functional significance and the pathways regulating their expression are largely unknown. We show that transfer of encephalitogenic CD4(+) Th17 cells is sufficient to induce CXCL1 and CXCL2 transcription in the spinal cords of naive, syngeneic recipients. Blockade or genetic silencing of CXCR2, a major receptor for these chemokines in mice, abrogates blood-brain barrier (BBB) breakdown, CNS infiltration by leukocytes, and the development of clinical deficits during the presentation as well as relapses of EAE. Depletion of circulating polymorphonuclear leukocytes (PMN) had a similar therapeutic effect. Furthermore, injection of CXCR2(+) PMN into CXCR2(-/-) mice was sufficient to restore susceptibility to EAE. Our findings reveal that a Th17-ELR(+) CXC chemokine pathway is critical for granulocyte mobilization, BBB compromise, and the clinical manifestation of autoimmune demyelination in myelin peptide-sensitized mice, and suggest new therapeutic targets for diseases such as MS.


Subject(s)
Autoimmune Diseases of the Nervous System/immunology , Autoimmune Diseases of the Nervous System/pathology , Central Nervous System/immunology , Central Nervous System/pathology , Chemokine CXCL1/immunology , Chemokine CXCL2/immunology , T-Lymphocytes, Helper-Inducer/immunology , Adoptive Transfer , Animals , Autoimmune Diseases of the Nervous System/prevention & control , Blood-Brain Barrier/immunology , Blood-Brain Barrier/pathology , Bone Marrow Cells/immunology , Inflammation , Leukocytes, Mononuclear/immunology , Mice , Mice, Inbred BALB C , Myelin Sheath/immunology , Receptors, Interleukin-8B/deficiency , Recurrence
12.
Neurosci Lett ; 368(1): 63-7, 2004 Sep 16.
Article in English | MEDLINE | ID: mdl-15342135

ABSTRACT

Various ganglioside immunization protocols were examined to refine the procedure for establishing an animal model of acute motor axonal neuropathy. The most effective was subcutaneous injection of an emulsion of 2.5mg of bovine brain ganglioside mixtures, keyhole lympet hemocyanin, and complete Freund's adjuvant to Japanese white rabbits, repeated at 3-week intervals. Under that protocol, all the rabbits developed marked flaccid paralysis associated with plasma anti-GM1 IgG antibody. This acute motor axonal neuropathy rabbit model also could be reproduced by the use of incomplete Freund's adjuvant, methylated bovine serum albumin, and New Zealand white rabbits. These results provide useful information for the confirmation of and further research on the model.


Subject(s)
Autoimmune Diseases of the Nervous System/prevention & control , Axons/immunology , Immunization , Motor Neurons/immunology , Acute Disease , Animals , Autoimmune Diseases of the Nervous System/immunology , BCG Vaccine/therapeutic use , Cattle , Dose-Response Relationship, Drug , Female , Gangliosides/immunology , Gangliosidosis, GM1/immunology , Gangliosidosis, GM1/prevention & control , Hemocyanins/immunology , Male , Rabbits , Rats , Rats, Inbred Lew , Serum Albumin, Bovine/immunology
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