Subject(s)
Alveolitis, Extrinsic Allergic/etiology , Asthma, Occupational/etiology , Allergens/adverse effects , Alveolitis, Extrinsic Allergic/immunology , Alveolitis, Extrinsic Allergic/physiopathology , Animals , Asthma, Occupational/immunology , Asthma, Occupational/physiopathology , Avian Proteins/adverse effects , Columbiformes , Flour/adverse effects , Humans , Immunoglobulin E/immunology , Male , Middle Aged , Respiratory Function Tests , Zea mays/adverse effectsSubject(s)
Allergens/adverse effects , Avian Proteins/adverse effects , Egg Hypersensitivity/diagnosis , Eggs/adverse effects , Enterocolitis/diagnosis , Allergens/immunology , Animals , Avian Proteins/immunology , Chickens , Child, Preschool , Egg Hypersensitivity/immunology , Egg Hypersensitivity/pathology , Eggs/analysis , Enterocolitis/chemically induced , Enterocolitis/immunology , Enterocolitis/pathology , Female , Humans , Quail , Skin TestsABSTRACT
Ovalbumin (OVA), one of the major allergens in hen egg white, and has widespread use in experimental models of allergy. The aim of this research was to assess the effect of glycation and heat treatment on the potential allergenicity of OVA prepared from hen egg white. Secondary and tertiary structures of OVA were also characterised to show the relationship between potential allergenicity and the conformation of OVA after heating and glycation. Glycation significantly reduced the potential allergenicity of OVA tested with egg allergy patients' sera, which was caused by conformation changes. An increased IgG reactivity was measured using rabbit anti-OVA and was supposed to be caused by protein unfolding which exposed hidden epitopes. Heating reduced the potential allergenicity of OVA at the expense of increased IgG reactivity. It is suggested that conformational changes of OVA induced by glycation and controlled heating significantly reduced its potential allergenicity.
Subject(s)
Allergens/chemistry , Allergens/immunology , Avian Proteins/chemistry , Avian Proteins/immunology , Ovalbumin/chemistry , Ovalbumin/immunology , Allergens/adverse effects , Animals , Antibody Specificity , Avian Proteins/adverse effects , Chickens , Egg Hypersensitivity/immunology , Glycosylation , Hot Temperature , Humans , Immunoglobulin E/blood , Ovalbumin/adverse effects , Protein Conformation , Protein Structure, Secondary , Protein Structure, Tertiary , RabbitsABSTRACT
The objective of this study was to evaluate and compare the biocompatibility profiles of hyaluronic acid (HA) viscosupplements from avian and non-mammalian sources. Inflammatory and immune reactions were assessed in models of both clinically relevant and stringent immunological exposure conditions. Experiments were conducted to evaluate tissue reactions and immunological responses and assess antibody formation with the capacity to bind directly to and cross-react with the different viscosupplements. Mice were exposed to viscosupplements using the air pouch inflammation model and specific immunization using Freund's complete adjuvant (FCA). Murine pouch membrane tissue reactions revealed generally mild to moderate responses, with cellular infiltration and cytokine profiles of pouch tissue characteristic of predominantly fibroblastic responses rather than marked inflammatory reactions. In vitro testing indicated that pouch injections did not elicit detectable T-cell proliferative responses, while antibody assays revealed that mice immunized with viscosupplements in FCA and subsequently boosted were capable of mounting an antibody response with a range of specificities. High reactivity to avian serum albumin was seen in sera from mice injected with HA from an avian source, while low positive reactivity to the bacterial antigen Staphylococcal Lipotechoic Acid was observed in sera from mice injected with HA from bacterial sources. These specificities did not indicate any propensity to cross-react, suggesting that patients with adverse immune responses to HA from an avian source should be unresponsive to subsequent injection with HA from a non-avian source. Overall, the findings demonstrate that viscosupplements exhibit good biocompatibility profiles in the murine air pouch, but when provoked to elicit immunological reactions exhibit unique antigenic spectra. These findings suggest that an immunologically mediated immune reaction directed against avian proteins should not necessarily be a contraindication for the administration of non-avian viscosupplements.